SU1105500A1 - Fermenter - Google Patents

Abstract

A fermentor containing a tubular body consisting of a series of sections and equipped with a degasser and nozzles for feeding the nutrient medium, air and the output of the finished product, characterized in that, in order to increase the biomass output, in each section of the tubular compartment & there is a section made of a semi-impermeable film for transporting metabolic products and mixing the culture fluid and encased in a casing to form an annular chamber for supplying cooled dialysis fluid, while the annular chamber is connected to the impulse pump to create radial movements of the film.

Description

The invention relates to apparatus for carrying out fermentation processes and can be used in the microbiological, pharmaceutical and food industry.

A horizontal type tubular fermenter is known with a translational displacement of the culture medium comprising a body consisting of a number of sections, a cooling system, input units for nutrient medium, air and output for the finished product m.

The main disadvantage of this device is the lack of radial mixing, which leads to an uneven distribution of nutrients and temperature throughout the volume of the culture medium.

The closest to the one proposed is a fermenter, comprising a tubular body consisting of a number of sections and provided with a degasser and nutrient inlets. air and the output of the finished product 2. - -.

However, the known fermenter does not provide radial mixing of the substrate, which leads to its uneven distribution over the volume of the body. Another significant disadvantage is the impossibility of dialysis. These drawbacks do not allow for a sufficiently high biomass yield.

The purpose of the invention is to increase the yield of biomass.

The goal is achieved by the fact that in the fermenter containing a tubular body consisting of a number of sections and equipped with a degasser and nozzles for feeding the nutrient medium, air and outputting the finished product c. Each section of the tubular body has a section made of a semi-permeable film for diverting products of metabolism and moving the culture fluid and encased in a casing to form an annular chamber to supply cooled dialysis fluid, while the annular chamber is connected to a pulse pump to create radial displacements of the film.

FIG. 1 shows schematically a fermenter, a general view; in fig. 2 section, the maternity cut.

The fermenter consists of a tubular body 1 consisting of a series of sections

2, connected in series. Each section 2 has an inlet 3 and an outlet 4 collectors. On the inlet manifold 3 there are nozzles for entering the nutrient medium and inoculum 5, air 6, and on the outlet 4 - a nozzle 7 for biomass output. The housing in each section 2 has a section 8 made of a semi-impermeable material, such as a dialysis film for removal of metabolic products and enclosed in a cylindrical casing 9. The annular chamber 10 formed by the section 8 and the cylindrical casing 9 serves to supply cooled dialysis fluid and is connected to a pulse pump 11, for example, a membrane pump, for creating radial movements of the film. The inlet 3 and outlet 4 collectors are connected to the shaped flange 12 of the tubular body 1 by means of flange connections 13. On the shaped flange 12 there are fittings 14 for supplying and discharging dialysis fluid.

In each section 2, there are instruments 15 and 16 for monitoring the input and output parameters of the culture medium, installed respectively on the inlet 3 and outlet 4 collectors, the barbater 17, located in the inner cavity 18 of the section 8 of the tubular body 1, the degasser 19, which is a cavity connected to the vacuum system 20.

The fermenter works as follows.

After sterilization, nutrient medium and seed are fed into the cavity of the tubular housing 1. To ensure mixing, the impulse pump 11 creates a short-term periodic pressure increase in the annular chamber 10, which causes the film of section 8 of each section 2 to pulsate and the culture fluid in it. During the movement of the mixed and oxygen-enriched culture medium, the growth and development of microorganisms occurs, the control of which is carried out by instruments 15 and 16 of the control, installed at the entrance and exit of each section.

At the exit of each section 2, the medium is degassed to remove volatile metabolic products and the exhaust gases through the degasser 19 are removed from the fermenter. The degassed culture medium at the inlet to the last section is saturated with air oxygen through the barbuter 17, and the necessary nutrient components are supplied fractionally. The passage of a series of sections that provide different conditions for the growth of microbial populations from the last section of the culture fluid is removed by pipe 21. If necessary to remove a range of metabolic products, instead of the cooled liquid, a solution can be supplied to ensure the most complete dialysis of metabolic products from the culture environment. An example is the process of obtaining a therapeutic preparation of colibacterin, which is based on viable E.Co 11 M cells. 17. The process of periodic cell cultivation lasts six hours, during which the specific growth rate from the maximum in the first hour (1.0-1 , 2) decreases in the second and third to 0.6 hours, and then to the sixth hour - to 0.15. 0.20 h, the pH is maintained at a level of 7.6-7.7 over the entire period, with a rise to 8.0 pH units in the last sixth hour; fractional glucose addition in the first three hours is 25-30%, in the next two hours - 70-75% of the total volume of added glucose, aeration with air in volume mode per unit of volume of the culture medium all six hours without changes. The cell concentration increases exponentially from (0.5-0.7) x 109 at the beginning to (60-70) X 10 cells / ml at the end of the culture. Accordingly, after the first section 2 is filled with the nutrient medium with microorganisms, it is maintained at a temperature of 37 ° C and periodic radial stirring. 1 4i Then a channel is set equal to 1 hour, and the culture medium from the first section flows into the second. After the culture medium in this section has been delayed for another 1 hour (the time for complete filling of the internal cavity), the flow from this section is set at 0.5 hours. At this stage o6b (its cultivation time will be 3 hours and so on up to the last sections with a flow of 0.25 hours, the number of which is determined by the achievement of a given cell concentration in them. The use of a tubular torus enzyme allows for complex multi-stage fermentation processes in which maximum biomass accumulation and metabolic target product do not coincide in time. The maximum biomass yield is ensured by the possibility of creating at each stage of cultivation the necessary conditions for the growth of microbial populations This means that it is possible to reject energy-intensive mixing devices, simplify the processing technology of the inner surface of the body, and unify the fermenters for growing any hydrocarbon-containing ones.

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Claims (1)

FERMENTATOR, comprising a tubular casing, consisting of a number of sections and equipped with a degasser and nozzles for introducing a nutrient medium, air and outlet of the finished product, characterized in that, in order to increase the biomass output, each section of the tubular casing ^ has a section made of a semi-impermeable film for removal of metabolic products and mixing of the culture fluid and enclosed in a casing with the formation of an annular chamber for supplying cooled dialysis fluid, while the annular chamber is connected to a pulse suck to create radial movements of the film. Figure 1 SU p „1105500 1 1105500 2