SU1058281A1 - Panax cinseng ifrj2- producer of biologically active substances of ginsong and method of obtaining biologically active substances - Google Patents

Abstract

The strain Rapach ginseng IFR2 1 (stored in the collection of plant cell cultures of the Institute of Plant Physiology named after KA Timir zev of the USSR Academy of Sciences) produces biologically active substances of ginseng. 2. A method of obtaining biologically active substances by growing a producer strain on a nutrient medium containing micro and macro salts, growth stimulus, sugar and agargar followed by the allocation of the target product, characterized in that, in order to increase the biological activity of the target product in The producer’s source is used, the strain Rapach ginseng IFR2, and growing is carried out on a medium containing, MG / L WATER

Description

The invention relates to chi pharmaceuticals can be used in the dairy industry for preparing creams and lotions, as well as in a host. A method is known for producing biochemistry products that are biologically cultured on a nutrient medium. Composition 1J. The Rapach ginse producer is known for producing biologically active substances ginseng. Also known is the method of semi biologically active substances cultivating the producer strain on a nutrient medium containing mg / l of water: 1650 1900 440 MgS04-7HgO 370 170 KH2P04 6.20 HjBO, 22.80 MnSO 8.60 0.88 0.25 Na2MoO. - 0.025 CuSO5H20 CoCl2 O, 025 27.80 FeS04. 7H 0 Na EDTA 37.30 (sodium salt of ethylenediam of tetraacetic acid), Thiamine (B) 0.40 s - Naphthlucium c on acid (NUA) 2 Kinetin 1 Inositol 80 Casein hydrolyzate 500 Sugar Sugar 310 Agar-Agar. However, technically known solutions do not provide highly logical activity of the target product. The aim of the invention is the biological activity of the product. This goal is achieved that the method does not contain biologically active substances that provide for the growth of the producer on a nutrient medium that contains micro them krosoli with growth regulators, sugar and agar, followed vscheleniem desired product. The difference is that the Rapach ginseng IGF2 strain is used as a producer, and cultivation is carried out on a medium containing mg / l of water: 1145-1850 1490-2100 420-500 350-400 150-210 4.2-8-8.0 15.6-26.7 6.0-10.3 0.53-0.90 0.25-0.30 0.03-0.075 0.03-0.075 25.0-30.0, 26.1- 40.1 Na2 EDTA Thiaminbromide (W) 0.40-0.80 2.5 3.5 Yu Sakhar 6-10 - 7 .10 Agar-agar oi-Naphthyloacetic 1.0 - 3.0 acid To use the method proposed The strain Rapach ginseng IFR2 (collection number 1) is a producer of biologically active substances that are linked together by a single inventive concept. Strain Rapach ginseng IFRZh2 obtained at the Institute of Plant Physiology. K.A. Timir of the pharynx is stored in the collection of plant cell cultures of this Institute under the number 1. The strain is characterized by the following features. The strain is a creamy white, out of water, hydrated cell mass with a specific odor and taste. The population consists of 60-90% of cells of the meristematic type (from 252 to 92.4 µm) and 2-10% of parenchymal cells (from 75.6 to 201.6 µm). Lignification is up to 20% with the formation of conductive elements. Tissue growing on a medium, the composition of which is excluded from one of the growth regulators, kinetin, casein hydrolyzate and inositol, has good pocta intensity. Growth index is 19.5-16.5, dry matter content 3.3-4.0%. The sweep cycle is characterized by an S-shaped curve with a lag-phase up to 3-5 days, an exponential growth phase (5-10 days), a linear famax (10-33 days), a growth slowdown phase (32-35 days) and a stationary phase (35 -37 days). The curve of mitotic activity has a two-vertex character with a maximum in the first and three weeks. The average number of chromosomes is 7517.1 with a variation of 41 to 136. The use of the IFRS2 strain as a producer of biologically active substances of ginseng and the method of obtaining biologically active substances is illustrated by the following example. Example 1, In terms of a 250 ml capacity of 250 ml of 50 ml of the proposed nutrient medium, the following composition, mg / l water: 1145-1850 1490-2100 420-500 CaCl 2H20 350-400 MgS04 7H20 150-210 KN3P04 4, 2-8.0 15.6-26.7 MnS04 4HjO ZnSO 7H „0 6.0-10.3 KI. 0.53-0.90 Thiamin bro MFA (B,) 0.40-0.80 2.5 10 - 3.5-10f 6 -103 - 7.10 Agar-agar about (.- Naphthyloxy 1.0 - 3, 0 acid at pH 5.8, closed with cotton plugs and sterilized at a temperature of 15 minutes, then under sterile conditions, 1 g of the tissue of the Rapach ginseng strain IFR2 is placed on the surface of the agar medium. The flasks with an inoculum are incubated at 2 ° C and relative humidity 70% of the air. The growing cycle is 37 days. At the end of the growing period, the grown biomass is separated from the agar and weighed. Comparative data on the biomass yield are given in Table 1.

Biomass yield

After the expiration of the growth cycle, the biomass weighing 18.09 g is separated from the agar and dried in a drying oven at a temperature. The dried fabric is crushed to a particle size of 0.05-0.1 1-IM and used as the target product.

Obtaining biologically active substances.

100 g of crushed dry biomass of strain IHFR2 are placed in a percolator (1.0 l), 500 ml of 35% ethanol is poured over to form a mirror layer over the raw material and left for up to 24 hours. After the end of Table 1

For a certain period of time, the extract is percalated at a rate of 5 ml / min with simultaneous intake of 35% ethanol at the same rate at the same time. Extraction is continued until 1 liter is obtained. The resulting tincture is left for 2 days, filtered and used for its intended purpose as the desired product.

Pharmacological testing of biologically active substances from the IFRG and IGF2 stags was carried out on non-linear male mice.

The results are presented in table. Comparison of the biological activity of strains

 the ratio of running time (in min.) of control and experimental animals,%. conditional stimulating unit of action, providing an increase in working capacity by 33% from 1 g of raw material.

The use of shtamma-trodutsen biomass, reduce by 39% the cost of IFRZh2 biologically active programs for obtaining 1 kg of dry biomass of the panaxoside ginseng and fitness compared to the known method of panaxoside production and improve the life expectancy of the biologists active properties of target | production of substances due to increased output. T and l and c and 2 IFRZh1 and IFRZh2

Claims (3)

The strain Panax ginseng IFR2 No. 1 (stored in the collection of plant cell cultures of the Institute of Plant Physiology named after K.A. Timiryazev of the USSR Academy of Sciences) is a producer of biologically active substances of ginseng. ₍ 2. A method of producing biologically active substances by growing a producer strain on a nutrient medium containing micro and macro salts, growth stimulants, sugar and agaragar followed by injection of the target product, characterized in that, in order to increase the biological activity of the target product as a producer use the Panax ginseng strain IFRG2, and the cultivation is carried out on a medium containing, mg / l of water: νη ₄ νο ₃ KN0 ₃ CaCl ₂ MgSO ₄ -7H ₂ O ΚΗ, ΡΟ. m ₃ in ₃ MnS0 ₄ 4H ₂ About ZnS0 ₄ -7H ₂ 0 κα Na ₂ Mo0 ₄ · 2Η.0 CuS0 ₄ · SH ^ O CoCi. · 2H ₂ O FeSO ₄ 7H ₂ O Na ₂ , EDTA. Thiamine bromide (Bj) Sugar Agar-agar ot-Naphthylacetic acid 1145-1850 1490-2100 420-500 350-400 150-210 4.2-8.0 15.6-26.7 6.0-10.3 0.53-0.90 0.25-0.30 0.03-0.075 0.03-0.075 25.0-30.0 26.1-40.1 0.40-0.80 2.5 ΊΟ * - 3.5 ΊΟ * 6 ΊΟ '- 7ΊΟ ³ 1.0-3.0