SG11202113122SA - Methods and apparatuses for patient-derived micro-organospheres - Google Patents

Methods and apparatuses for patient-derived micro-organospheres

Info

Publication number
SG11202113122SA
SG11202113122SA SG11202113122SA SG11202113122SA SG11202113122SA SG 11202113122S A SG11202113122S A SG 11202113122SA SG 11202113122S A SG11202113122S A SG 11202113122SA SG 11202113122S A SG11202113122S A SG 11202113122SA SG 11202113122S A SG11202113122S A SG 11202113122SA
Authority
SG
Singapore
Prior art keywords
organospheres
patient
cells
micro
derived micro
Prior art date
Application number
SG11202113122SA
Other languages
English (en)
Inventor
Xiling Shen
Zhaouhui Wang
Daniel Delubac
Jeffrey Motschman
David Hsu
Original Assignee
Xiling Shen
Zhaouhui Wang
Daniel Delubac
Jeffrey Motschman
David Hsu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xiling Shen, Zhaouhui Wang, Daniel Delubac, Jeffrey Motschman, David Hsu filed Critical Xiling Shen
Publication of SG11202113122SA publication Critical patent/SG11202113122SA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0012—Cell encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/01—Drops
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/08—Chemical, biochemical or biological means, e.g. plasma jet, co-culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/50—Soluble polymers, e.g. polyethyleneglycol [PEG]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Oncology (AREA)
  • Rheumatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physiology (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
SG11202113122SA 2019-05-28 2020-04-01 Methods and apparatuses for patient-derived micro-organospheres SG11202113122SA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962853219P 2019-05-28 2019-05-28
PCT/US2020/026275 WO2020242594A1 (en) 2019-05-28 2020-04-01 Methods and apparatuses for patient-derived micro-organospheres

Publications (1)

Publication Number Publication Date
SG11202113122SA true SG11202113122SA (en) 2021-12-30

Family

ID=73549270

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202113122SA SG11202113122SA (en) 2019-05-28 2020-04-01 Methods and apparatuses for patient-derived micro-organospheres

Country Status (10)

Country Link
US (2) US11555180B2 (enExample)
EP (1) EP3976064A4 (enExample)
JP (1) JP2022534778A (enExample)
CN (1) CN116157134A (enExample)
AU (1) AU2020283390A1 (enExample)
CA (1) CA3142113A1 (enExample)
IL (1) IL288370A (enExample)
MX (1) MX2021014439A (enExample)
SG (1) SG11202113122SA (enExample)
WO (1) WO2020242594A1 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114410466A (zh) * 2020-04-01 2022-04-29 沈西凌 用于个性化癌症治疗的精确药物筛选
WO2022115455A1 (en) * 2020-11-24 2022-06-02 Duke University Droplet organoid-based immuno-oncology assays and methods of using same
DE102022100146B4 (de) 2022-01-04 2023-11-30 Precomb Therapeutics Ag Verfahren zur analyse von personalisierten anti-krebs-wirkstoffen in zellkulturen
JP2025514291A (ja) * 2022-04-29 2025-05-02 ザイリス， インク． マイクロオルガノスフェアを使用して肝細胞毒性を試験するための方法及び装置
EP4519686A4 (en) * 2022-05-03 2026-05-06 Xilis Inc Patient-Derived Microorganospheres (MOS) Enabling Clinical Precision Oncology
WO2024039770A2 (en) * 2022-08-17 2024-02-22 Xilis, Inc. Imaging-based microorganosphere drug assays
JP2025532497A (ja) * 2022-09-07 2025-10-01 ザイリス， インク． 多発性骨髄腫ＭｉｃｒｏＯｒｇａｎｏＳｐｈｅｒｅ
EP4601758A1 (en) 2022-10-11 2025-08-20 Xilis, Inc. Integrated microfluidic system for generation of microorganospheres (mos)

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5266480A (en) 1986-04-18 1993-11-30 Advanced Tissue Sciences, Inc. Three-dimensional skin culture system
PT671923E (pt) 1992-10-09 2001-10-30 Advanced Tissue Sciences Inc Celulas de reserva do figado
WO2005047863A2 (en) 2003-11-10 2005-05-26 Platypus Technologies, Llc Substrates, devices, and methods for cellular assays
US7759111B2 (en) * 2004-08-27 2010-07-20 The Regents Of The University Of California Cell encapsulation microfluidic device
WO2006078814A2 (en) 2005-01-20 2006-07-27 The Regents Of The University Of California Cellular microarrays for screening differentiation factors
US20070134209A1 (en) * 2005-12-12 2007-06-14 Metafluidics, Inc. Cellular encapsulation for self-assembly of engineered tissue
US20120135875A1 (en) * 2008-02-29 2012-05-31 Bristol-Myers Squibb Company Methods for determining sensitivity to vascular endothelial growth factor receptor-2 modulators
CN103635587A (zh) 2008-04-08 2014-03-12 麻省理工学院 三维微流平台和其使用方法
WO2012018982A2 (en) * 2010-08-05 2012-02-09 Tufts Medical Center, Inc. Methods and kits for modulating tumor invasiveness and metastatic potential
GB201105226D0 (en) * 2011-03-29 2011-05-11 Univ Leiden Methods
SG10201805199RA (en) * 2011-06-02 2018-07-30 Harvard College Methods and Uses for Ex Vivo Tissue Culture Systems
US9334473B2 (en) 2012-08-17 2016-05-10 Jelena Vukasinovic Three dimensional cell culture compositions and methods of use
WO2014082096A1 (en) * 2012-11-26 2014-05-30 The Trustees Of Columbia University In The City Of New York Method for culture of human and mouse prostate organoids and uses thereof
KR102257305B1 (ko) 2013-03-28 2021-05-28 더 유니버시티 오브 브리티쉬 콜롬비아 미세유동 장치 및 분비물의 다세포 검정법에서의 그의 사용 방법
AU2014275006A1 (en) * 2013-06-04 2015-12-24 University Of Miami Assays, methods and kits for analyzing sensitivity and resistance to anti-cancer drugs, predicting a cancer patient's prognosis, and personalized treatment strategies
WO2015027086A1 (en) 2013-08-21 2015-02-26 University Of Louisville Research Foundation, Inc. Methods of making spheroids including biologically-relevant materials
US10928382B2 (en) * 2014-06-26 2021-02-23 Northeastern University Microfluidic device and method for analysis of tumor cell microenvironments
EP3242930A4 (en) 2015-01-07 2019-01-16 Dana-Farber Cancer Institute, Inc. MICROFLUIDIC CELL CULTURE OF TUMOR CELL SPHEROIDS FROM PATIENTS
WO2016145242A1 (en) * 2015-03-11 2016-09-15 Agenus Inc. Methods and compositions for high throughput screening of biomolecules using gel microdrops
AU2016346332B2 (en) * 2015-10-26 2022-03-03 University Of Wyoming Methods of generating microparticles and porous hydrogels using microfluidics
TWM516858U (zh) 2015-11-19 2016-02-11 金鼎聯合科技纖維股份有限公司 膠線改良結構
GB2564582B (en) 2016-02-01 2021-09-22 Emulate Inc Systems and methods for growth of intestinal cells in microfluidic devices
JP6967535B2 (ja) * 2016-06-15 2021-11-17 ミメタス ビー．ブイ． 細胞培養装置及び方法
EP3484487A4 (en) * 2016-07-12 2020-06-17 The University of North Carolina at Chapel Hill BIOMATRIX SCALES FOR USE IN THE DIAGNOSIS AND MODELING OF CANCER
US11180735B2 (en) * 2016-10-28 2021-11-23 The Board Of Trustees Of The Leland Stanford Junior University Methods to preserve tumor-stromal interactions in culture and therapeutic predictive applications thereof
CN110997900B (zh) * 2017-07-14 2024-06-14 多伦多大学管理委员会 用于快速产生用于化合物筛选的类器官/球状体的微流体平台
WO2019067795A1 (en) 2017-09-27 2019-04-04 Duke University PATIENT-SPECIFIC CLINICAL TRIALS AND METHODS OF TREATMENT THEREOF
US11166920B2 (en) * 2017-10-05 2021-11-09 Auburn University Microfluidics device for fabrication of large, uniform, injectable hydrogel microparticles for cell encapsulation
WO2019111998A1 (ja) * 2017-12-08 2019-06-13 京ダイアグノスティクス株式会社 癌スフェロイドの製造方法および大腸癌患者の選択方法
GB201721615D0 (en) * 2017-12-21 2018-02-07 Koninklijke Nederlandse Akademie Van Wetenschappen Immune cell organoid co-cultures
CN110004111B (zh) * 2019-04-22 2021-10-08 清华-伯克利深圳学院筹备办公室 一种类器官球体的制备方法

Also Published As

Publication number Publication date
EP3976064A1 (en) 2022-04-06
US11555180B2 (en) 2023-01-17
JP2022534778A (ja) 2022-08-03
AU2020283390A1 (en) 2021-12-23
IL288370A (en) 2022-01-01
MX2021014439A (es) 2022-05-19
US20200377861A1 (en) 2020-12-03
CN116157134A (zh) 2023-05-23
WO2020242594A1 (en) 2020-12-03
CA3142113A1 (en) 2020-12-03
US20230002737A1 (en) 2023-01-05
EP3976064A4 (en) 2023-05-31

Similar Documents

Publication Publication Date Title
US20230002737A1 (en) Methods and Apparatuses for Patient-Derived Micro-Organospheres
US20210285054A1 (en) Precision drug screening for personalized cancer therapy
US20220062791A1 (en) Methods and apparatuses for purification of gel droplets supporting biological tissue
US20230003716A1 (en) Methods and apparatuses for patient-derived microorganospheres
JP2023553838A (ja) 個人化された医薬品及び薬剤の開発で使用される有機マイクロ球状体
WO2022119966A1 (en) Precision drug screening for personalized cancer therapy
US20260055431A1 (en) Methods of delivering components to microorganospheres
US20240385173A1 (en) Methods and apparatus for testing immuno-oncology drugs and biologics using microorganospheres
US20250320462A1 (en) Methods and apparatuses for testing hepatocyte toxicity using microorganospheres
HK40074891A (en) Precision drug screening for personalized cancer therapy
US20260078348A1 (en) Multiple myeloma microorganospheres
CN116615654A (zh) 基于液滴类器官的免疫肿瘤学分析及其使用方法
EP4677359A1 (en) Cell labeling and annotation in micro-organospheres