CA3142113A1 - Methods and apparatuses for patient-derived micro-organospheres - Google Patents

Methods and apparatuses for patient-derived micro-organospheres Download PDF

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Publication number
CA3142113A1
CA3142113A1 CA3142113A CA3142113A CA3142113A1 CA 3142113 A1 CA3142113 A1 CA 3142113A1 CA 3142113 A CA3142113 A CA 3142113A CA 3142113 A CA3142113 A CA 3142113A CA 3142113 A1 CA3142113 A1 CA 3142113A1
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CA
Canada
Prior art keywords
patient
organospheres
cells
micro
derived micro
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3142113A
Other languages
English (en)
French (fr)
Inventor
Xiling Shen
Zhaouhui WANG
Daniel DELUBAC
Jeffrey MOTSCHMAN
David Hsu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Duke University
Xilis Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA3142113A1 publication Critical patent/CA3142113A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/01—Drops
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/08—Chemical, biochemical or biological means, e.g. plasma jet, co-culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0012—Cell encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/50—Soluble polymers, e.g. polyethyleneglycol [PEG]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Oncology (AREA)
  • Rheumatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physiology (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA3142113A 2019-05-28 2020-04-01 Methods and apparatuses for patient-derived micro-organospheres Pending CA3142113A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962853219P 2019-05-28 2019-05-28
US62/853,219 2019-05-28
PCT/US2020/026275 WO2020242594A1 (en) 2019-05-28 2020-04-01 Methods and apparatuses for patient-derived micro-organospheres

Publications (1)

Publication Number Publication Date
CA3142113A1 true CA3142113A1 (en) 2020-12-03

Family

ID=73549270

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3142113A Pending CA3142113A1 (en) 2019-05-28 2020-04-01 Methods and apparatuses for patient-derived micro-organospheres

Country Status (10)

Country Link
US (2) US11555180B2 (enExample)
EP (1) EP3976064A4 (enExample)
JP (1) JP2022534778A (enExample)
CN (1) CN116157134A (enExample)
AU (1) AU2020283390A1 (enExample)
CA (1) CA3142113A1 (enExample)
IL (1) IL288370A (enExample)
MX (1) MX2021014439A (enExample)
SG (1) SG11202113122SA (enExample)
WO (1) WO2020242594A1 (enExample)

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CN114410466A (zh) * 2020-04-01 2022-04-29 沈西凌 用于个性化癌症治疗的精确药物筛选
WO2022115455A1 (en) * 2020-11-24 2022-06-02 Duke University Droplet organoid-based immuno-oncology assays and methods of using same
DE102022100146B4 (de) 2022-01-04 2023-11-30 Precomb Therapeutics Ag Verfahren zur analyse von personalisierten anti-krebs-wirkstoffen in zellkulturen
JP2025514291A (ja) * 2022-04-29 2025-05-02 ザイリス， インク． マイクロオルガノスフェアを使用して肝細胞毒性を試験するための方法及び装置
EP4519686A4 (en) * 2022-05-03 2026-05-06 Xilis Inc Patient-Derived Microorganospheres (MOS) Enabling Clinical Precision Oncology
WO2024039770A2 (en) * 2022-08-17 2024-02-22 Xilis, Inc. Imaging-based microorganosphere drug assays
JP2025532497A (ja) * 2022-09-07 2025-10-01 ザイリス， インク． 多発性骨髄腫ＭｉｃｒｏＯｒｇａｎｏＳｐｈｅｒｅ
EP4601758A1 (en) 2022-10-11 2025-08-20 Xilis, Inc. Integrated microfluidic system for generation of microorganospheres (mos)

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US20070134209A1 (en) * 2005-12-12 2007-06-14 Metafluidics, Inc. Cellular encapsulation for self-assembly of engineered tissue
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AU2016346332B2 (en) * 2015-10-26 2022-03-03 University Of Wyoming Methods of generating microparticles and porous hydrogels using microfluidics
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Also Published As

Publication number Publication date
EP3976064A1 (en) 2022-04-06
US11555180B2 (en) 2023-01-17
JP2022534778A (ja) 2022-08-03
AU2020283390A1 (en) 2021-12-23
IL288370A (en) 2022-01-01
MX2021014439A (es) 2022-05-19
US20200377861A1 (en) 2020-12-03
CN116157134A (zh) 2023-05-23
SG11202113122SA (en) 2021-12-30
WO2020242594A1 (en) 2020-12-03
US20230002737A1 (en) 2023-01-05
EP3976064A4 (en) 2023-05-31

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