SG11202102025RA - A method for direct reprogramming of urine cells into nueral stem cells using synthetic mrna - Google Patents

A method for direct reprogramming of urine cells into nueral stem cells using synthetic mrna

Info

Publication number
SG11202102025RA
SG11202102025RA SG11202102025RA SG11202102025RA SG11202102025RA SG 11202102025R A SG11202102025R A SG 11202102025RA SG 11202102025R A SG11202102025R A SG 11202102025RA SG 11202102025R A SG11202102025R A SG 11202102025RA SG 11202102025R A SG11202102025R A SG 11202102025RA
Authority
SG
Singapore
Prior art keywords
cells
nueral
synthetic mrna
urine
direct reprogramming
Prior art date
Application number
SG11202102025RA
Other languages
English (en)
Inventor
Seung Kwon You
Phil Jun Kang
Da Ryeon Son
Won Jun Hong
Won Jin Yun
Jang Bo Lee
Gyu Man Park
In Yong Kim
Jung Hyun Park
Jie Zheng
wei wei Gao
Ji Hoon Jang
Eun Kyoung Jun
Byung Sun Yoon
Original Assignee
Stemlab Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stemlab Inc filed Critical Stemlab Inc
Publication of SG11202102025RA publication Critical patent/SG11202102025RA/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/065—Modulators of histone acetylation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/41—Hedgehog proteins; Cyclopamine (inhibitor)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/603—Oct-3/4
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/604—Klf-4
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/73—Hydrolases (EC 3.)
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/25—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from renal cells, from cells of the urinary tract
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2510/00—Genetically modified cells
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Ophthalmology & Optometry (AREA)
  • Epidemiology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Hospice & Palliative Care (AREA)
  • Psychiatry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
SG11202102025RA 2018-08-28 2019-08-28 A method for direct reprogramming of urine cells into nueral stem cells using synthetic mrna SG11202102025RA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020180101208A KR102143320B1 (ko) 2018-08-28 2018-08-28 합성 메신저 rna를 이용하여 소변세포를 신경줄기세포로 직접 역분화하는 방법
PCT/KR2019/011014 WO2020045990A1 (ko) 2018-08-28 2019-08-28 합성 메신저 rna를 이용하여 소변세포를 신경줄기세포로 직접 역분화하는 방법

Publications (1)

Publication Number Publication Date
SG11202102025RA true SG11202102025RA (en) 2021-04-29

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202102025RA SG11202102025RA (en) 2018-08-28 2019-08-28 A method for direct reprogramming of urine cells into nueral stem cells using synthetic mrna

Country Status (12)

Country Link
US (1) US20210260132A1 (zh)
EP (1) EP3845637A4 (zh)
JP (3) JP2022503604A (zh)
KR (1) KR102143320B1 (zh)
CN (1) CN113015793A (zh)
AU (1) AU2019328089B2 (zh)
BR (1) BR112021003791A2 (zh)
CA (1) CA3111313A1 (zh)
IL (1) IL281153A (zh)
IN (1) IN202137009932A (zh)
SG (1) SG11202102025RA (zh)
WO (1) WO2020045990A1 (zh)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114736864B (zh) * 2020-12-23 2024-01-26 武汉睿健医药科技有限公司 一种神经干细胞诱导分化培养基及诱导分化方法
WO2023090268A1 (ja) * 2021-11-17 2023-05-25 武田薬品工業株式会社 細胞の製造方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5634609B2 (ja) 2010-09-16 2014-12-03 ハチソン メディファーマ リミテッド 縮合ヘテロアリールおよびその使用
WO2012074117A1 (ja) * 2010-12-03 2012-06-07 国立大学法人京都大学 効率的な人工多能性幹細胞の樹立方法
CN102604894B (zh) * 2012-02-29 2014-07-30 中国科学院广州生物医药与健康研究院 用于制备神经干细胞的培养基及其用途
SG11201407595UA (en) 2012-05-21 2014-12-30 Univerisity Of California Generation of human ips cells by a synthetic self- replicative rna
AU2013274197A1 (en) * 2012-06-13 2015-01-22 Stemgent, Inc. Methods of preparing pluripotent stem cells
US10077429B2 (en) * 2012-10-23 2018-09-18 Kyoto University Method of efficiently establishing induced pluripotent stem cells
US11047848B2 (en) * 2013-03-15 2021-06-29 Whitehead Institute For Biomedical Research Cellular discovery platform for neurodegenerative diseases
KR20140121317A (ko) * 2013-04-06 2014-10-15 서울대학교산학협력단 비신경 세포로부터 유도 신경 줄기 세포의 생산 방법 및 이에 의하여 생산되는 유도 신경 줄기 세포
CN104630136B (zh) * 2013-11-15 2019-10-01 中国科学院广州生物医药与健康研究院 一种制备诱导多能性干细胞的方法以及该方法中所使用的组合物及其应用

Also Published As

Publication number Publication date
JP2022503604A (ja) 2022-01-12
CA3111313A1 (en) 2020-03-05
US20210260132A1 (en) 2021-08-26
KR20200024478A (ko) 2020-03-09
EP3845637A1 (en) 2021-07-07
CN113015793A (zh) 2021-06-22
JP2024074958A (ja) 2024-05-31
WO2020045990A1 (ko) 2020-03-05
JP2023037004A (ja) 2023-03-14
AU2019328089A1 (en) 2021-03-18
EP3845637A4 (en) 2022-06-15
IL281153A (en) 2021-04-29
IN202137009932A (zh) 2021-04-02
AU2019328089B2 (en) 2023-08-10
BR112021003791A2 (pt) 2021-05-18
KR102143320B1 (ko) 2020-08-12

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