SG11202012807YA - Methods and compositions for paired end sequencing using a single surface primer - Google Patents

Methods and compositions for paired end sequencing using a single surface primer

Info

Publication number
SG11202012807YA
SG11202012807YA SG11202012807YA SG11202012807YA SG11202012807YA SG 11202012807Y A SG11202012807Y A SG 11202012807YA SG 11202012807Y A SG11202012807Y A SG 11202012807YA SG 11202012807Y A SG11202012807Y A SG 11202012807YA SG 11202012807Y A SG11202012807Y A SG 11202012807YA
Authority
SG
Singapore
Prior art keywords
compositions
methods
single surface
end sequencing
paired end
Prior art date
Application number
SG11202012807YA
Other languages
English (en)
Inventor
Jonathan Mark Boutell
Pietro Gatti-Lafranconi
Original Assignee
Illumina Cambridge Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
Publication of SG11202012807YA publication Critical patent/SG11202012807YA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
SG11202012807YA 2018-12-18 2019-12-09 Methods and compositions for paired end sequencing using a single surface primer SG11202012807YA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862781273P 2018-12-18 2018-12-18
PCT/EP2019/084183 WO2020126602A1 (en) 2018-12-18 2019-12-09 Methods and compositions for paired end sequencing using a single surface primer

Publications (1)

Publication Number Publication Date
SG11202012807YA true SG11202012807YA (en) 2021-01-28

Family

ID=68887016

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202012807YA SG11202012807YA (en) 2018-12-18 2019-12-09 Methods and compositions for paired end sequencing using a single surface primer

Country Status (9)

Country Link
US (2) US11634765B2 (https=)
EP (1) EP3899041A1 (https=)
JP (1) JP7587425B2 (https=)
KR (1) KR102872035B1 (https=)
CN (1) CN112739830A (https=)
AU (1) AU2019411272B2 (https=)
CA (1) CA3103633A1 (https=)
SG (1) SG11202012807YA (https=)
WO (1) WO2020126602A1 (https=)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11202012762WA (en) 2018-12-05 2021-01-28 Illumina Cambridge Ltd Methods and compositions for cluster generation by bridge amplification
US12359193B2 (en) 2022-03-04 2025-07-15 Element Biosciences, Inc. Single-stranded splint strands and methods of use
US12371743B2 (en) 2022-03-04 2025-07-29 Element Biosciences, Inc. Double-stranded splint adaptors and methods of use
CN115803451A (zh) * 2020-09-11 2023-03-14 伊鲁米纳公司 用于检测核酸的无碱基位点的组合物和方法
US11859241B2 (en) 2021-06-17 2024-01-02 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
US11220707B1 (en) 2021-06-17 2022-01-11 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
WO2022266470A1 (en) * 2021-06-17 2022-12-22 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
KR20240047357A (ko) * 2021-06-17 2024-04-12 엘리먼트 바이오사이언스, 인크. 쌍별 서열분석을 위한 조성물 및 방법
CN114457146B (zh) * 2022-01-28 2024-11-19 赛纳生物科技(北京)有限公司 一种固相介质表面双端扩增测序的方法
CN118272513A (zh) * 2022-01-28 2024-07-02 赛纳生物科技(北京)有限公司 一种芯片表面双端扩增测序的方法
CA3223037A1 (en) * 2022-03-30 2023-10-05 Oliver MILLER Methods for chemical cleavage of surface-bound polynucleotides
EP4551718A1 (en) 2022-07-05 2025-05-14 Element Biosciences, Inc. Pcr-free library preparation using double-stranded splint adaptors and methods of use
AU2023325064A1 (en) 2022-08-15 2025-03-06 Element Biosciences, Inc. Spatially resolved surface capture of nucleic acids
AU2023343187A1 (en) 2022-09-12 2025-03-13 Element Biosciences, Inc. Double-stranded splint adaptors with universal long splint strands and methods of use

Family Cites Families (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8810400D0 (en) 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
US5552278A (en) 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US6023540A (en) 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
JP2002503954A (ja) 1997-04-01 2002-02-05 グラクソ、グループ、リミテッド 核酸増幅法
DE19812103A1 (de) 1998-03-19 1999-09-23 Bernauer Annette Verfahren zur Synthese von Nucleinsäuremolekülen
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US20050181440A1 (en) 1999-04-20 2005-08-18 Illumina, Inc. Nucleic acid sequencing using microsphere arrays
EP1923471B1 (en) 1999-04-20 2012-12-19 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US6913884B2 (en) 2001-08-16 2005-07-05 Illumina, Inc. Compositions and methods for repetitive use of genomic DNA
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
EP1990428B1 (en) 2000-02-07 2010-12-22 Illumina, Inc. Nucleic acid detection methods using universal priming
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
WO2002004680A2 (en) 2000-07-07 2002-01-17 Visigen Biotechnologies, Inc. Real-time sequence determination
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
EP3002289B1 (en) 2002-08-23 2018-02-28 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
DE20214753U1 (de) 2002-09-24 2002-12-12 Hando Handels Ges. M.B.H., Graz Pharmazeutische Zusammensetzung zur Behandlung und/oder Prävention einer Lichtdermatose
CA2528577C (en) 2003-06-20 2012-08-07 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
EP1701785A1 (en) 2004-01-07 2006-09-20 Solexa Ltd. Modified molecular arrays
US7315019B2 (en) 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
WO2006064199A1 (en) 2004-12-13 2006-06-22 Solexa Limited Improved method of nucleotide detection
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
DK1987159T4 (da) 2006-02-08 2020-11-16 Illumina Cambridge Ltd Fremgangsmåde til sekventering af en polynukleotidtemplate
EP3722409A1 (en) 2006-03-31 2020-10-14 Illumina, Inc. Systems and devices for sequence by synthesis analysis
US8071308B2 (en) 2006-05-04 2011-12-06 Alere San Diego, Inc. Recombinase polymerase amplification
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US7948015B2 (en) 2006-12-14 2011-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US8198023B2 (en) 2008-08-05 2012-06-12 Pacific Biosciences Of California, Inc. Prevention and alleviation of steric hindrance during single molecule nucleic acid synthesis by a polymerase
US8586310B2 (en) 2008-09-05 2013-11-19 Washington University Method for multiplexed nucleic acid patch polymerase chain reaction
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US9029103B2 (en) 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
EP2632593B1 (en) 2010-10-27 2021-09-29 Illumina, Inc. Flow cells for biological or chemical analysis
EP3564392B1 (en) 2010-12-17 2021-11-24 Life Technologies Corporation Methods for nucleic acid amplification
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
EP2718465B1 (en) 2011-06-09 2022-04-13 Illumina, Inc. Method of making an analyte array
US9139874B2 (en) * 2011-07-07 2015-09-22 Life Technologies Corporation Bi-directional sequencing compositions and methods
CA2859660C (en) 2011-09-23 2021-02-09 Illumina, Inc. Methods and compositions for nucleic acid sequencing
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
WO2014031163A1 (en) * 2012-08-24 2014-02-27 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid paired end sequencing
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
WO2016054096A1 (en) 2014-09-30 2016-04-07 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
DK3218511T3 (da) 2014-11-11 2020-07-20 Illumina Cambridge Ltd Fremgangsmåder og arrays til frembringelse og sekventering af monoklonale klynger af nukleinsyre
SG10202104816QA (en) 2015-02-10 2021-06-29 Illumina Inc Methods and compositions for analyzing cellular components
CN117822128A (zh) 2016-07-22 2024-04-05 俄勒冈健康与科学大学 单细胞全基因组文库及制备其的组合索引方法
CA3059840C (en) 2017-04-23 2022-04-26 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
FI3842545T3 (fi) 2017-04-23 2023-01-31 Koostumuksia ja menetelmiä näytteiden tunnistuksen parantamiseksi indeksoiduissa nukleiinihappokirjastoissa
EP3615671B1 (en) 2017-04-23 2021-07-21 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
EP3872187B1 (en) 2017-04-23 2022-09-21 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
SG11202012762WA (en) 2018-12-05 2021-01-28 Illumina Cambridge Ltd Methods and compositions for cluster generation by bridge amplification

Also Published As

Publication number Publication date
WO2020126602A1 (en) 2020-06-25
AU2019411272A1 (en) 2021-01-07
US20200190578A1 (en) 2020-06-18
AU2019411272B2 (en) 2025-08-14
CN112739830A (zh) 2021-04-30
JP7587425B2 (ja) 2024-11-20
KR102872035B1 (ko) 2025-10-16
CA3103633A1 (en) 2020-06-25
US20230137106A1 (en) 2023-05-04
US11634765B2 (en) 2023-04-25
EP3899041A1 (en) 2021-10-27
KR20210104554A (ko) 2021-08-25
JP2022513546A (ja) 2022-02-09

Similar Documents

Publication Publication Date Title
SG11202012807YA (en) Methods and compositions for paired end sequencing using a single surface primer
GB2574746B (en) Engineered polymerases for improved sequencing
IL286089A (en) RNA-guided DNA integration using tn7-like transposons
GB2581629B (en) Sequencing for pipe handling
GB202107805D0 (en) Riser tools and methods
SG11202012758WA (en) Primer oligonucleotide for sequencing
PT3721027T (pt) Nivelador para ladrilhos
GB2572953B8 (en) Surface Analysis
PL3900573T3 (pl) Urządzenie do zdobienia paznokci
GB201713300D0 (en) Surface modification using abrasive blasting
ZA202101188B (en) Grip assist apparatus with insert
HK40054238A (en) Methods and compositions for paired end sequencing using a single surface primer
SG11202012753SA (en) Sequencing kits
LT3640304T (lt) Dažų ir tinko gruntas
CA189443S (en) Trowel
GB2589106B (en) A nail holding apparatus
HK40080943A (en) Methods and devices using cartridges for sequencing
GB201813171D0 (en) Sequencing method
GB201907101D0 (en) Sequencing method
GB201919362D0 (en) Paint easy
HK40055931A (en) Primer oligonucleotide for sequencing
GB2577060B (en) Adaptor
GB2577325B (en) Releasable clamp
IL266492B (en) Compsite grip/slat-armor
GB2562793B (en) Improved movement joint for between tiles and coatings