CA3103633A1 - Methods and compositions for paired end sequencing using a single surface primer - Google Patents

Methods and compositions for paired end sequencing using a single surface primer Download PDF

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Publication number
CA3103633A1
CA3103633A1 CA3103633A CA3103633A CA3103633A1 CA 3103633 A1 CA3103633 A1 CA 3103633A1 CA 3103633 A CA3103633 A CA 3103633A CA 3103633 A CA3103633 A CA 3103633A CA 3103633 A1 CA3103633 A1 CA 3103633A1
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CA
Canada
Prior art keywords
strand
amplification
nucleic acid
nucleotides
sequencing
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Application number
CA3103633A
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English (en)
French (fr)
Inventor
Jonathan Mark Boutell
Pietro GATTI-LAFRANCONI
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Illumina Cambridge Ltd
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Illumina Cambridge Ltd
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Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
Publication of CA3103633A1 publication Critical patent/CA3103633A1/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA3103633A 2018-12-18 2019-12-09 Methods and compositions for paired end sequencing using a single surface primer Pending CA3103633A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862781273P 2018-12-18 2018-12-18
US62/781,273 2018-12-18
PCT/EP2019/084183 WO2020126602A1 (en) 2018-12-18 2019-12-09 Methods and compositions for paired end sequencing using a single surface primer

Publications (1)

Publication Number Publication Date
CA3103633A1 true CA3103633A1 (en) 2020-06-25

Family

ID=68887016

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3103633A Pending CA3103633A1 (en) 2018-12-18 2019-12-09 Methods and compositions for paired end sequencing using a single surface primer

Country Status (9)

Country Link
US (2) US11634765B2 (https=)
EP (1) EP3899041A1 (https=)
JP (1) JP7587425B2 (https=)
KR (1) KR102872035B1 (https=)
CN (1) CN112739830A (https=)
AU (1) AU2019411272B2 (https=)
CA (1) CA3103633A1 (https=)
SG (1) SG11202012807YA (https=)
WO (1) WO2020126602A1 (https=)

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Also Published As

Publication number Publication date
WO2020126602A1 (en) 2020-06-25
AU2019411272A1 (en) 2021-01-07
US20200190578A1 (en) 2020-06-18
AU2019411272B2 (en) 2025-08-14
CN112739830A (zh) 2021-04-30
JP7587425B2 (ja) 2024-11-20
KR102872035B1 (ko) 2025-10-16
US20230137106A1 (en) 2023-05-04
US11634765B2 (en) 2023-04-25
EP3899041A1 (en) 2021-10-27
KR20210104554A (ko) 2021-08-25
SG11202012807YA (en) 2021-01-28
JP2022513546A (ja) 2022-02-09

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