SG11202008080RA - Ligation mediated analysis of nucleic acids - Google Patents

Ligation mediated analysis of nucleic acids

Info

Publication number
SG11202008080RA
SG11202008080RA SG11202008080RA SG11202008080RA SG11202008080RA SG 11202008080R A SG11202008080R A SG 11202008080RA SG 11202008080R A SG11202008080R A SG 11202008080RA SG 11202008080R A SG11202008080R A SG 11202008080RA SG 11202008080R A SG11202008080R A SG 11202008080RA
Authority
SG
Singapore
Prior art keywords
nucleic acids
ligation mediated
mediated analysis
analysis
ligation
Prior art date
Application number
SG11202008080RA
Other languages
English (en)
Inventor
Tarjei Sigurd Mikkelsen
Eswar Prasad Ramachandran Iyer
Andrew Kohlway
Luigi Jhon Alvarado Martinez
Katherine Pfeiffer
Andrew D Price
Original Assignee
10X Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 10X Genomics Inc filed Critical 10X Genomics Inc
Publication of SG11202008080RA publication Critical patent/SG11202008080RA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/682Signal amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855Ligating adaptors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
SG11202008080RA 2018-02-22 2019-02-22 Ligation mediated analysis of nucleic acids SG11202008080RA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862633982P 2018-02-22 2018-02-22
US201962804648P 2019-02-12 2019-02-12
PCT/US2019/019309 WO2019165318A1 (en) 2018-02-22 2019-02-22 Ligation mediated analysis of nucleic acids

Publications (1)

Publication Number Publication Date
SG11202008080RA true SG11202008080RA (en) 2020-09-29

Family

ID=65952052

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202008080RA SG11202008080RA (en) 2018-02-22 2019-02-22 Ligation mediated analysis of nucleic acids

Country Status (6)

Country Link
US (3) US20200239874A1 (zh)
EP (2) EP3755812A1 (zh)
CN (2) CN112074610A (zh)
AU (1) AU2020335919A1 (zh)
SG (1) SG11202008080RA (zh)
WO (2) WO2019165318A1 (zh)

Families Citing this family (108)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103998394B (zh) 2011-08-01 2016-08-17 德诺弗科学公司 细胞捕获系统和使用方法
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR102190198B1 (ko) 2013-02-08 2020-12-14 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
MX2016016902A (es) 2014-06-26 2017-03-27 10X Genomics Inc Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
JP6735348B2 (ja) 2016-02-11 2020-08-05 10エックス ジェノミクス, インコーポレイテッド 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
EP3651903A4 (en) 2017-08-29 2021-06-16 Bio-Rad Laboratories, Inc. SYSTEM AND METHOD FOR ISOLATING AND ANALYZING CELLS
US10590244B2 (en) 2017-10-04 2020-03-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
EP3700672B1 (en) 2017-10-27 2022-12-28 10X Genomics, Inc. Methods for sample preparation and analysis
EP3625361A1 (en) 2017-11-15 2020-03-25 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
SG11202008080RA (en) * 2018-02-22 2020-09-29 10X Genomics Inc Ligation mediated analysis of nucleic acids
EP3775271A1 (en) 2018-04-06 2021-02-17 10X Genomics, Inc. Systems and methods for quality control in single cell processing
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
EP3894587A1 (en) 2018-12-10 2021-10-20 10X Genomics, Inc. Resolving spatial arrays by proximity-based deconvolution
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
WO2020168013A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
US11920183B2 (en) 2019-03-11 2024-03-05 10X Genomics, Inc. Systems and methods for processing optically tagged beads
US10633693B1 (en) 2019-04-16 2020-04-28 Celsee Diagnostics, Inc. System and method for leakage control in a particle capture system
US11273439B2 (en) 2019-05-07 2022-03-15 Bio-Rad Laboratories, Inc. System and method for target material retrieval from microwells
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
EP4025709A1 (en) * 2019-09-06 2022-07-13 10X Genomics, Inc. Systems and methods for barcoding cells and cell beads
WO2021091859A1 (en) * 2019-11-04 2021-05-14 Bio-Rad Laboratories, Inc. Cell barcoding for single cell sequencing
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
JP2023504836A (ja) * 2019-12-06 2023-02-07 バイオ-ラッド ラボラトリーズ インコーポレイテッド サンプル処理バーコード付きビーズ組成物、方法、製造方法およびシステム
EP3891300B1 (en) 2019-12-23 2023-03-29 10X Genomics, Inc. Methods for spatial analysis using rna-templated ligation
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US20210238664A1 (en) * 2020-02-03 2021-08-05 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
CN115485391A (zh) * 2020-02-17 2022-12-16 10X基因组学有限公司 染色质相互作用的原位分析
AU2021225020A1 (en) 2020-02-21 2022-08-18 10X Genomics, Inc. Methods and compositions for integrated in situ spatial assay
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US20210270703A1 (en) 2020-02-28 2021-09-02 10X Genomics, Inc. Method for isolating nuclei and cells from tissues
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
WO2021216708A1 (en) 2020-04-22 2021-10-28 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
WO2021247568A1 (en) 2020-06-02 2021-12-09 10X Genomics, Inc. Spatial trancriptomics for antigen-receptors
AU2021283174A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Nucleic acid library methods
CN116234926A (zh) * 2020-06-03 2023-06-06 中国海洋大学 分析来自细胞的目标核酸的方法
EP4162074B1 (en) 2020-06-08 2024-04-24 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
WO2021252591A1 (en) 2020-06-10 2021-12-16 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
CN116034166A (zh) 2020-06-25 2023-04-28 10X基因组学有限公司 Dna甲基化的空间分析
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US20220042097A1 (en) * 2020-08-04 2022-02-10 The Broad Institute, Inc. In-situ spatial transcriptomics and proteomics
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
WO2022178304A1 (en) 2021-02-19 2022-08-25 10X Genomics, Inc. High-throughput methods for analyzing and affinity-maturing an antigen-binding molecule
WO2022182682A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
WO2022182672A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Single cell glycan profiling
EP4301870A1 (en) 2021-03-18 2024-01-10 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
WO2022221428A1 (en) 2021-04-14 2022-10-20 10X Genomics, Inc. Compositions and methods for single cell analyte detection and analysis
EP4347870A1 (en) * 2021-05-24 2024-04-10 Singleron Biotechnologies Inc. Methods and systems for determining cell-cell interaction
WO2022256345A1 (en) 2021-06-01 2022-12-08 10X Genomics, Inc. Methods and systems for engineering antibodies, and antigen-binding fragments thereof, to have altered characteristics
WO2022271908A1 (en) 2021-06-23 2022-12-29 10X Genomics, Inc. Chop-fix method and chopping device for preparing biological samples
WO2023009988A1 (en) * 2021-07-26 2023-02-02 10X Genomics, Inc. Nucleic acid processing via circularization
WO2023022925A1 (en) 2021-08-17 2023-02-23 10X Genomics, Inc. Compositions, systems and methods for enzyme optimization
EP4196605A1 (en) 2021-09-01 2023-06-21 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2023060110A1 (en) 2021-10-05 2023-04-13 10X Genomics, Inc. Methods of immune cell analysis
WO2023215861A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Reagents for characterizing antigen-binding molecules from immune cells
WO2023225259A1 (en) 2022-05-20 2023-11-23 10X Genomics, Inc. Compositions and methods for characterizing antigen binding molecules from single cells
WO2023225201A1 (en) 2022-05-20 2023-11-23 10X Genomics, Inc. Compositions and methods for characterizing t cell, or t cell-like, receptors from single cells
WO2023225294A1 (en) 2022-05-20 2023-11-23 10X Genomics, Inc. Improved major histocompatibility complex molecules
WO2023235596A1 (en) 2022-06-03 2023-12-07 10X Genomics, Inc. Systems and methods for determining antigen binding specificity of antigen binding molecules
WO2023235570A1 (en) 2022-06-03 2023-12-07 10X Genomics, Inc. Methods and compositions for the identification of antigen binding molecules using lipoparticle-based antigen mapping
WO2023250422A1 (en) 2022-06-23 2023-12-28 10X Genomics, Inc. Compositions and methods for characterizing multispecific antigen binding molecules from single cells
WO2024006734A1 (en) 2022-06-27 2024-01-04 10X Genomics, Inc. Methods for preparing and using mhc multimer reagents compositions
WO2024006392A1 (en) 2022-06-29 2024-01-04 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
WO2024015856A1 (en) 2022-07-13 2024-01-18 10X Genomics, Inc. Compositions and methods for characterizing binding characteristics of antigen binding molecules from single cells
WO2024015378A1 (en) 2022-07-13 2024-01-18 10X Genomics, Inc. Methods and systems for characterizing antigen-binding molecules expressed by immune cells
WO2024015733A1 (en) 2022-07-13 2024-01-18 10X Genomics, Inc. Improved methods and systems for identification and characterization of antigen-binding molecules from single cells
WO2024050299A1 (en) 2022-08-29 2024-03-07 10X Genomics, Inc. Improved methods and compositions for characterization of antigen-binding molecules from single cells
US20240068010A1 (en) * 2022-08-31 2024-02-29 Genomill Health Oy Highly sensitive methods for accurate parallel quantification of variant nucleic acids
WO2024076908A1 (en) 2022-10-03 2024-04-11 10X Genomics, Inc. Compositions and methods for analyzing genomic insertion sites of exogenous nucleic acids

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005021794A2 (en) * 2003-09-02 2005-03-10 Keygene N.V. Ola-based methods for the detection of target nucleic acid sequences
US9012390B2 (en) 2006-08-07 2015-04-21 Raindance Technologies, Inc. Fluorocarbon emulsion stabilizing surfactants
US20120034603A1 (en) * 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
WO2013192292A1 (en) * 2012-06-21 2013-12-27 Justin Lamb Massively-parallel multiplex locus-specific nucleic acid sequence analysis
US20140378345A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
AU2013302756C1 (en) 2012-08-14 2018-05-17 10X Genomics, Inc. Microcapsule compositions and methods
CA2935122C (en) * 2013-12-30 2023-09-19 Atreca, Inc. Analysis of nucleic acids associated with single cells using nucleic acid barcodes
DE202015009494U1 (de) 2014-04-10 2018-02-08 10X Genomics, Inc. Fluidische Vorrichtungen und Systeme zur Einkapselung und Partitionierung von Reagenzien, und deren Anwendungen
MX2016016902A (es) 2014-06-26 2017-03-27 10X Genomics Inc Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.
WO2016135800A1 (ja) * 2015-02-23 2016-09-01 株式会社日立製作所 発電システム
US11092607B2 (en) * 2015-10-28 2021-08-17 The Board Institute, Inc. Multiplex analysis of single cell constituents
CN108026568A (zh) * 2016-01-31 2018-05-11 阿费梅特里克斯公司 通过结合条形码标记的多核苷酸探针进行核酸分析
CN114875125A (zh) * 2016-10-19 2022-08-09 10X基因组学有限公司 用于条形码化单个细胞或细胞群的核酸分子的方法和系统
EP3574112B1 (en) * 2017-01-27 2023-01-11 Roche Diagnostics GmbH Barcoded dna for long range sequencing
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US10590244B2 (en) 2017-10-04 2020-03-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
SG11202008080RA (en) * 2018-02-22 2020-09-29 10X Genomics Inc Ligation mediated analysis of nucleic acids
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing

Also Published As

Publication number Publication date
WO2021041974A1 (en) 2021-03-04
EP3755812A1 (en) 2020-12-30
US20210040551A1 (en) 2021-02-11
WO2019165318A9 (en) 2019-09-26
CN112074610A (zh) 2020-12-11
CN114616341A (zh) 2022-06-10
US20200239874A1 (en) 2020-07-30
AU2020335919A1 (en) 2022-03-17
WO2019165318A1 (en) 2019-08-29
US20210230584A1 (en) 2021-07-29
EP4022084A1 (en) 2022-07-06

Similar Documents

Publication Publication Date Title
SG11202008080RA (en) Ligation mediated analysis of nucleic acids
EP3717645A4 (en) NUCLEIC ACID SEQUENCING BY EMERGENCE
IL262409A (en) Improved methods for modification of targeted nucleic acids
GB2590032B (en) Determination of base modifications of nucleic acids
IL270825A (en) Multiplexed amplification with end-labeling of nucleic acids
DK3882357T3 (da) Fremgangsmåder og sammensætninger til analyse af nukleinsyrer
PT3529380T (pt) Métodos para ligação química de ácidos nucléicos
EP3414327A4 (en) DETECTION OF NUCLEIC ACIDS
EP3402883A4 (en) COMPOSITIONS AND METHODS FOR NUCLEIC ACID SEQUENCING
IL280772A (en) Multiplex testing of nucleic acids
EP3583112A4 (en) COMPOSITIONS AND METHODS FOR THE SEQUENCING OF NUCLEIC ACIDS
EP3344767A4 (en) SMALL MOLECULAR MEDIATED SIZE SELECTION OF NUCLEIC ACIDS
EP3638813A4 (en) ISOLATION OF TARGET NUCLEIC ACIDS
GB201804642D0 (en) Methods of labelling nucleic acids
EP3650553C0 (en) METHOD FOR DETECTING SPECIFIC NUCLEIC ACIDS
IL281941A (en) Nucleic acid labeling
EP4061958A4 (en) METHOD FOR SEQUENTIAL DETECTION OF NUCLEIC ACIDS
EP3906314A4 (en) IMPROVED AMPLIFICATION OF NUCLEIC ACIDS
GB201914266D0 (en) Method of amplifying nucleic acids
AU2018902915A0 (en) Multiplex detection of nucleic acids
HK1253363A1 (zh) 用於治療變態反應的核酸
GB201808882D0 (en) Nucleic acids and uses thereof
GB201706801D0 (en) Base-modified nucleic acids
GB201706802D0 (en) Base-modified nucleic acids