SG11202006232SA - Adeno-associated virus purification methods - Google Patents
Adeno-associated virus purification methodsInfo
- Publication number
- SG11202006232SA SG11202006232SA SG11202006232SA SG11202006232SA SG11202006232SA SG 11202006232S A SG11202006232S A SG 11202006232SA SG 11202006232S A SG11202006232S A SG 11202006232SA SG 11202006232S A SG11202006232S A SG 11202006232SA SG 11202006232S A SG11202006232S A SG 11202006232SA
- Authority
- SG
- Singapore
- Prior art keywords
- adeno
- associated virus
- purification methods
- virus purification
- methods
- Prior art date
Links
- 241000702421 Dependoparvovirus Species 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 238000000746 purification Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
- B01D15/166—Fluid composition conditioning, e.g. gradient
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3804—Affinity chromatography
- B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
- B01D15/424—Elution mode
- B01D15/426—Specific type of solvent
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14151—Methods of production or purification of viral material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/01—DNA viruses
- G01N2333/075—Adenoviridae
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Preparation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762611709P | 2017-12-29 | 2017-12-29 | |
| PCT/US2018/067627 WO2019133677A1 (en) | 2017-12-29 | 2018-12-27 | Adeno-associated virus purification methods |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11202006232SA true SG11202006232SA (en) | 2020-07-29 |
Family
ID=67064154
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11202006232SA SG11202006232SA (en) | 2017-12-29 | 2018-12-27 | Adeno-associated virus purification methods |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US11981934B2 (https=) |
| EP (1) | EP3731863A4 (https=) |
| JP (2) | JP7299896B2 (https=) |
| KR (1) | KR20200106045A (https=) |
| CN (1) | CN111655285B (https=) |
| AU (1) | AU2018395254B2 (https=) |
| BR (1) | BR112020013197A8 (https=) |
| CA (1) | CA3084827A1 (https=) |
| IL (1) | IL275533B2 (https=) |
| MX (1) | MX2020006884A (https=) |
| SG (1) | SG11202006232SA (https=) |
| WO (1) | WO2019133677A1 (https=) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2019299964A1 (en) * | 2018-07-11 | 2021-01-21 | Takeda Pharmaceutical Company Limited | AAV compositions |
| EP3990031A4 (en) * | 2019-06-28 | 2023-08-09 | Takeda Pharmaceutical Company Limited | Adeno-associated virus purification methods |
| EP3868886A1 (en) * | 2020-02-21 | 2021-08-25 | Bia Separations D.O.O. | A method for separation or depletion of empty aav capsids from full aav capsids |
| US20220033782A1 (en) * | 2020-07-29 | 2022-02-03 | Pall Corporation | Adenovirus-associated viruses separation method |
| JP2023539163A (ja) * | 2020-08-18 | 2023-09-13 | ザルトリウス ビーアイエー セパレーションズ ディー．オー．オー． | 宿主細胞ｄｎａの金属アフィニティー抽出 |
| JP2023548816A (ja) * | 2020-11-03 | 2023-11-21 | ファイザー・インク | 陰イオン交換クロマトグラフィーによるａａｖベクターの精製方法 |
| US20230416693A1 (en) * | 2020-11-24 | 2023-12-28 | Sartorius Bia Separations D.O.O. | Improved washing methods for affinity chromatography |
| JP2024503234A (ja) * | 2020-12-23 | 2024-01-25 | ファイザー・インク | 親和性クロマトグラフィーによるａａｖベクターの精製方法 |
| EP4281552A4 (en) * | 2021-01-21 | 2025-03-12 | AskBio Inc. | Method for purifying recombinant viral particles |
| WO2022222869A1 (zh) * | 2021-04-19 | 2022-10-27 | 信念医药（香港）有限公司 | 重组腺相关病毒及其应用 |
| CN114591921B (zh) * | 2022-03-31 | 2024-08-02 | 上海勉亦生物科技有限公司 | 腺相关病毒的纯化方法和洗脱液 |
| GB202110014D0 (en) * | 2021-07-12 | 2021-08-25 | Cytiva Bioprocess R & D Ab | A method for separating adeno-associated virus capsids, compositions obtained by said method and uses thereof |
| CN113552349B (zh) * | 2021-07-30 | 2022-07-19 | 上海勉亦生物科技有限公司 | Aav蛋白外壳的检测方法 |
| US20230183659A1 (en) * | 2021-10-29 | 2023-06-15 | Homology Medicines, Inc. | Methods and compositions for the purification of adeno-associated virus |
| CN114350622A (zh) * | 2022-01-17 | 2022-04-15 | 苏州博腾生物制药有限公司 | 一种病毒纯化高效保护试剂及其应用 |
| EP4490179A1 (en) | 2022-03-07 | 2025-01-15 | Takeda Pharmaceutical Company Limited | Affinity chromatographic production of clinical human igg products |
| GB202206123D0 (en) * | 2022-04-27 | 2022-06-08 | Cytiva Bioprocess R & D Ab | A pre-screening method and a method for separating adeno-associated virus capsids |
| WO2024233992A2 (en) * | 2023-05-11 | 2024-11-14 | Trophogen, Inc. | Methods of manufacturing highly glycosylated glycoprotein hormones and their analogs |
| WO2025027058A1 (en) * | 2023-08-01 | 2025-02-06 | Merck Patent Gmbh | Polishing for aav particles using anion exchange chromatography with improved elution system |
| WO2025104341A1 (en) * | 2023-11-16 | 2025-05-22 | Sartorius Bia Separations D.O.O. | Enhanced chromatographic separation of components in a mixture by employing decreasing gradient of complexing agent |
| WO2025207456A1 (en) * | 2024-03-25 | 2025-10-02 | Frontera Therapeutics, Inc. | Methods for purification of adeno associated virus particles by anion exchange chromatography |
| WO2026077794A1 (en) * | 2024-10-07 | 2026-04-16 | Cytiva Us Llc | Plasmid purification methods |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4543363A (en) | 1983-06-15 | 1985-09-24 | Asahi Kasei Kogyo Kabushiki Kaisha | Ion exchanger having hydroxyl groups bonded directly to backbone skeleton |
| SE9902133D0 (sv) | 1999-06-06 | 1999-06-06 | Amersham Pharm Biotech Ab | A method for surface modification, a novel support matrix and the use of the matrix |
| GB9925432D0 (en) * | 1999-10-27 | 1999-12-29 | Univ London | Virus preparation |
| US6593123B1 (en) | 2000-08-07 | 2003-07-15 | Avigen, Inc. | Large-scale recombinant adeno-associated virus (rAAV) production and purification |
| HUE028341T2 (en) | 2009-06-16 | 2016-12-28 | Genzyme Corp | Improved methods for purification of recombinant aav vectors |
| US20150275195A1 (en) * | 2012-10-24 | 2015-10-01 | Genzyme Corporation | Elution of biomolecules from multi-modal resins using mes and mops as mobile phase modifiers |
| CN104232687A (zh) * | 2014-09-17 | 2014-12-24 | 许瑞安 | 一种重组腺相关病毒rAAV载体的分离纯化方法 |
| EP3054007A1 (en) * | 2015-02-09 | 2016-08-10 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Recombinant adeno-associated virus particle purification comprising an immuno-affinity purification step |
| WO2017100676A1 (en) | 2015-12-11 | 2017-06-15 | The Trustees Of The University Of Pennsylvania | Scalable purification method for aav8 |
| EP3387138B1 (en) | 2015-12-11 | 2022-01-26 | The Trustees Of The University Of Pennsylvania | Scalable purification method for aav9 |
| KR20220100017A (ko) | 2019-11-21 | 2022-07-14 | 이엠디 밀리포어 코포레이션 | 친수성 막 |
-
2018
- 2018-12-27 SG SG11202006232SA patent/SG11202006232SA/en unknown
- 2018-12-27 EP EP18895008.3A patent/EP3731863A4/en active Pending
- 2018-12-27 IL IL275533A patent/IL275533B2/en unknown
- 2018-12-27 WO PCT/US2018/067627 patent/WO2019133677A1/en not_active Ceased
- 2018-12-27 CN CN201880087932.0A patent/CN111655285B/zh active Active
- 2018-12-27 US US16/958,974 patent/US11981934B2/en active Active
- 2018-12-27 MX MX2020006884A patent/MX2020006884A/es unknown
- 2018-12-27 CA CA3084827A patent/CA3084827A1/en active Pending
- 2018-12-27 BR BR112020013197A patent/BR112020013197A8/pt unknown
- 2018-12-27 JP JP2020536656A patent/JP7299896B2/ja active Active
- 2018-12-27 KR KR1020207021627A patent/KR20200106045A/ko not_active Ceased
- 2018-12-27 AU AU2018395254A patent/AU2018395254B2/en active Active
-
2023
- 2023-04-26 JP JP2023072157A patent/JP7531654B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| MX2020006884A (es) | 2020-10-01 |
| KR20200106045A (ko) | 2020-09-10 |
| EP3731863A1 (en) | 2020-11-04 |
| JP2023089298A (ja) | 2023-06-27 |
| JP7299896B2 (ja) | 2023-06-28 |
| RU2020124946A (ru) | 2022-01-31 |
| WO2019133677A1 (en) | 2019-07-04 |
| BR112020013197A2 (pt) | 2021-02-17 |
| AU2018395254A1 (en) | 2020-06-25 |
| CA3084827A1 (en) | 2019-07-04 |
| US11981934B2 (en) | 2024-05-14 |
| US20200332266A1 (en) | 2020-10-22 |
| EP3731863A4 (en) | 2021-10-27 |
| AU2018395254B2 (en) | 2024-07-25 |
| IL275533B1 (en) | 2024-11-01 |
| CN111655285A (zh) | 2020-09-11 |
| JP2021508484A (ja) | 2021-03-11 |
| BR112020013197A8 (pt) | 2022-11-08 |
| IL275533B2 (en) | 2025-03-01 |
| JP7531654B2 (ja) | 2024-08-09 |
| IL275533A (en) | 2020-08-31 |
| RU2020124946A3 (https=) | 2022-02-08 |
| CN111655285B (zh) | 2025-02-25 |
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