SG11201903550TA - Protein screening and detection method - Google Patents
Protein screening and detection methodInfo
- Publication number
- SG11201903550TA SG11201903550TA SG11201903550TA SG11201903550TA SG11201903550TA SG 11201903550T A SG11201903550T A SG 11201903550TA SG 11201903550T A SG11201903550T A SG 11201903550TA SG 11201903550T A SG11201903550T A SG 11201903550TA SG 11201903550T A SG11201903550T A SG 11201903550TA
- Authority
- SG
- Singapore
- Prior art keywords
- library
- international
- rule
- nested
- deepsequencing
- Prior art date
Links
- 238000001514 detection method Methods 0.000 title abstract 7
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- 102000004169 proteins and genes Human genes 0.000 title abstract 2
- 238000012216 screening Methods 0.000 title abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 7
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 7
- 229920001184 polypeptide Polymers 0.000 abstract 6
- 238000012350 deep sequencing Methods 0.000 abstract 4
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- 238000004949 mass spectrometry Methods 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 239000013598 vector Substances 0.000 abstract 2
- 108010003723 Single-Domain Antibodies Proteins 0.000 abstract 1
- 238000010828 elution Methods 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 230000008520 organization Effects 0.000 abstract 1
- 239000013600 plasmid vector Substances 0.000 abstract 1
- 101150048568 pnl gene Proteins 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 238000012163 sequencing technique Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/22—Immunoglobulins specific features characterized by taxonomic origin from camelids, e.g. camel, llama or dromedary
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
- C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/22—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a Strep-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
- C07K2319/41—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a Myc-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
- C07K2319/43—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a FLAG-tag
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/119—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being proteinic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP16196571 | 2016-10-31 | ||
| PCT/EP2017/077816 WO2018078167A1 (en) | 2016-10-31 | 2017-10-30 | Protein screening and detection method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201903550TA true SG11201903550TA (en) | 2019-05-30 |
Family
ID=57280982
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201903550TA SG11201903550TA (en) | 2016-10-31 | 2017-10-30 | Protein screening and detection method |
Country Status (16)
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9345495B2 (en) | 2010-08-16 | 2016-05-24 | Smith & Nephew, Inc. | Systems and methods for altering the surface of a bone |
| JP6683867B1 (ja) * | 2019-07-22 | 2020-04-22 | 植田 充美 | モノクローナル抗体の酵母による製造方法およびスクリーニング方法 |
| WO2024026026A1 (en) * | 2022-07-27 | 2024-02-01 | Trustees Of Tufts College | High throughput in vivo screening of lipid nanoparticles |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AUPN480095A0 (en) | 1995-08-15 | 1995-09-07 | Commonwealth Scientific And Industrial Research Organisation | Epitope tagging system |
| AUPP727398A0 (en) | 1998-11-20 | 1998-12-17 | Commonwealth Scientific And Industrial Research Organisation | Methods of identifying antigen gene sequences |
| JP2003536048A (ja) * | 1999-03-23 | 2003-12-02 | バイオヴェイション リミテッド | タンパク質の単離および分析 |
| US20090233806A1 (en) * | 1999-07-06 | 2009-09-17 | Carr Francis J | Protein isolation and analysis |
| GB0217402D0 (en) | 2002-07-26 | 2002-09-04 | Roslin Inst Edinburgh | Multi-reporter gene model for toxicological screening |
| JP2004329040A (ja) * | 2003-05-01 | 2004-11-25 | Daiichi Fine Chemical Co Ltd | ポリセラーゼ−ｉ及びその利用 |
| JP2007502417A (ja) * | 2003-08-11 | 2007-02-08 | モノグラム バイオサイエンシズ，インコーポレーテッド | 分子複合体の検出および特徴づけ |
| US20060073506A1 (en) * | 2004-09-17 | 2006-04-06 | Affymetrix, Inc. | Methods for identifying biological samples |
| US8148085B2 (en) * | 2006-05-15 | 2012-04-03 | Sea Lane Biotechnologies, Llc | Donor specific antibody libraries |
| WO2007134327A2 (en) | 2006-05-15 | 2007-11-22 | Sea Lane Biotechnologies, Llc. | Neutralizing antibodies to influenza viruses |
| EP2436766A1 (en) * | 2010-09-29 | 2012-04-04 | Deutsches Krebsforschungszentrum | Means and methods for improved protein interaction screening |
| EP2970952B1 (en) * | 2013-03-14 | 2018-07-11 | Adagene Inc. | An integrated system for library construction, affinity binder screening and expression thereof |
| US11339434B2 (en) * | 2016-07-29 | 2022-05-24 | The Regents Of The University Of California | Methods for determining gene functions |
| CN108593753B (zh) * | 2016-11-25 | 2020-06-05 | 北京毅新博创生物科技有限公司 | 通过内部标准物质谱检测微生物的内标校正方法 |
-
2017
- 2017-10-30 SG SG11201903550TA patent/SG11201903550TA/en unknown
- 2017-10-30 IL IL266270A patent/IL266270B2/en unknown
- 2017-10-30 HU HUE17797583A patent/HUE059755T2/hu unknown
- 2017-10-30 CN CN201780080285.6A patent/CN110225973B/zh active Active
- 2017-10-30 KR KR1020197014386A patent/KR102264642B1/ko active Active
- 2017-10-30 EP EP17797583.6A patent/EP3532612B1/en active Active
- 2017-10-30 US US16/345,895 patent/US12054708B2/en active Active
- 2017-10-30 EP EP22181179.7A patent/EP4123022A1/en active Pending
- 2017-10-30 EA EA201991019A patent/EA201991019A1/ru unknown
- 2017-10-30 AU AU2017351810A patent/AU2017351810B2/en active Active
- 2017-10-30 DK DK17797583.6T patent/DK3532612T3/da active
- 2017-10-30 ES ES17797583T patent/ES2926548T3/es active Active
- 2017-10-30 PL PL17797583.6T patent/PL3532612T3/pl unknown
- 2017-10-30 WO PCT/EP2017/077816 patent/WO2018078167A1/en active Application Filing
- 2017-10-30 JP JP2019522559A patent/JP7185929B2/ja active Active
- 2017-10-30 PT PT177975836T patent/PT3532612T/pt unknown
- 2017-10-30 CA CA3041406A patent/CA3041406A1/en active Pending
-
2024
- 2024-06-17 US US18/745,866 patent/US20240327825A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP3532612B1 (en) | 2022-06-29 |
| EP4123022A1 (en) | 2023-01-25 |
| PT3532612T (pt) | 2022-07-26 |
| US20190276819A1 (en) | 2019-09-12 |
| EP3532612A1 (en) | 2019-09-04 |
| EA201991019A1 (ru) | 2020-01-22 |
| IL266270A (en) | 2019-06-30 |
| IL266270B2 (en) | 2025-01-01 |
| CN110225973B (zh) | 2024-02-13 |
| WO2018078167A1 (en) | 2018-05-03 |
| KR102264642B1 (ko) | 2021-06-15 |
| JP2020502493A (ja) | 2020-01-23 |
| AU2017351810A1 (en) | 2019-05-16 |
| ES2926548T3 (es) | 2022-10-26 |
| PL3532612T3 (pl) | 2022-10-31 |
| JP7185929B2 (ja) | 2022-12-08 |
| AU2017351810B2 (en) | 2023-07-20 |
| CA3041406A1 (en) | 2018-05-03 |
| HUE059755T2 (hu) | 2022-12-28 |
| KR20190104312A (ko) | 2019-09-09 |
| CN110225973A (zh) | 2019-09-10 |
| IL266270B1 (en) | 2024-09-01 |
| US20240327825A1 (en) | 2024-10-03 |
| DK3532612T3 (da) | 2022-09-19 |
| US12054708B2 (en) | 2024-08-06 |
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