SG11201907162TA - Methods for cell-type specific profiling to identify drug targets - Google Patents

Methods for cell-type specific profiling to identify drug targets

Info

Publication number
SG11201907162TA
SG11201907162TA SG11201907162TA SG11201907162TA SG11201907162TA SG 11201907162T A SG11201907162T A SG 11201907162TA SG 11201907162T A SG11201907162T A SG 11201907162TA SG 11201907162T A SG11201907162T A SG 11201907162TA SG 11201907162T A SG11201907162T A SG 11201907162TA
Authority
SG
Singapore
Prior art keywords
new york
international
cell
pct
methods
Prior art date
Application number
SG11201907162TA
Inventor
Nathaniel Heintz
Xiao Xu
Original Assignee
Univ Rockefeller
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Rockefeller filed Critical Univ Rockefeller
Publication of SG11201907162TA publication Critical patent/SG11201907162TA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 16 August 2018 (16.08.2018) WIP0 1 PCT O II111110vDIIIIII (10) International Publication Number WO 2018/148501 Al (51) International Patent Classification: Cl2N15/11 (2006.01) C12Q 1/68 (2018.01) (21) International Application Number: PCT/US2018/017556 (22) International Filing Date: 09 February 2018 (09.02.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/457,420 10 February 2017 (10.02.2017) US 62/558,396 14 September 2017 (14.09.2017) US (71) Applicant: THE ROCKEFELLER UNIVERSITY [US/US]; Box 138, 1230 York Avenue, New York, New York 10065-6399 (US). (72) Inventors: HEINTZ, Nathaniel; c/o The Rockefeller Uni- versity, Box 138, 1230 York Avenue, New York, New York 10065-6399 (US). XU, Xiao; 500 E. 63rd St., Apt 15A, New York, New York 10065 (US). (74) Agent: WARD, Donna T. et al.; DT WARD, P.C., 142A MAIN STREET, GROTON, Massachusetts 01450 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). (54) Title: METHODS FOR CELL-TYPE SPECIFIC PROFILING TO IDENTIFY DRUG TARGETS FIG. 1-1 1-1 (57) : The present invention provides methods of profiling gene and protein expression of a plurality of nuclei from a single cell type and comparing the profiles to determine variability among cell populations, samples from different subjects, and cells expressing ei a disease phenotype. [Continued on next page] C WO 2018/148501 Al MIDEDIMOM0101011EFIEOMMIUMEINIIMOIllii0VOIMIE Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a))
SG11201907162TA 2017-02-10 2018-02-09 Methods for cell-type specific profiling to identify drug targets SG11201907162TA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762457420P 2017-02-10 2017-02-10
US201762558396P 2017-09-14 2017-09-14
PCT/US2018/017556 WO2018148501A1 (en) 2017-02-10 2018-02-09 Methods for cell-type specific profiling to identify drug targets

Publications (1)

Publication Number Publication Date
SG11201907162TA true SG11201907162TA (en) 2019-09-27

Family

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SG11201907162TA SG11201907162TA (en) 2017-02-10 2018-02-09 Methods for cell-type specific profiling to identify drug targets

Country Status (9)

Country Link
EP (1) EP3580338A4 (en)
JP (2) JP7229538B2 (en)
CN (1) CN110582578B (en)
AU (1) AU2018219308A1 (en)
CA (1) CA3052290A1 (en)
IL (1) IL268417A (en)
MX (1) MX2019009612A (en)
SG (1) SG11201907162TA (en)
WO (1) WO2018148501A1 (en)

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TW202043256A (en) 2019-01-10 2020-12-01 美商健生生物科技公司 Prostate neoantigens and their uses
CN114555827A (en) * 2019-08-12 2022-05-27 使命生物公司 Methods, systems and devices for simultaneous multiomic detection of protein expression, single nucleotide variation and copy number variation in the same single cell
KR20220052946A (en) * 2019-09-06 2022-04-28 각고호우징 게이오기주크 Method for producing cell aggregates containing glial progenitor cells
US11628222B2 (en) 2019-10-14 2023-04-18 Aro Biotherapeutics Company CD71 binding fibronectin type III domains
WO2021076574A2 (en) 2019-10-14 2021-04-22 Aro Biotherapeutics Company Fn3 domain-sirna conjugates and uses thereof
CN110699362B (en) * 2019-11-15 2023-03-24 兰州理工大学 AFP5 gene and application thereof
CN111312330A (en) * 2020-02-13 2020-06-19 兰州理工大学 Key protein identification method and system based on protein node characteristics
CN112538480B (en) * 2020-12-24 2022-09-27 广州市妇女儿童医疗中心（广州市妇幼保健院、广州市儿童医院、广州市妇婴医院、广州市妇幼保健计划生育服务中心） Construction method and application of animal model of sperm flagellum multiple morphologically abnormal disease
CN112522425B (en) * 2020-12-25 2023-11-28 武汉睿健医药科技有限公司 Marker gene for DPC cell identification and classification, screening method and application
CN112481273B (en) * 2020-12-29 2023-11-24 南通大学附属医院 Colorectal cancer inhibiting gene and verification method for high DNA methylation of promoter region of colorectal cancer inhibiting gene
CA3215367A1 (en) * 2021-04-14 2022-10-20 Swapnil Kulkarni Fn3 domain-sirna conjugates and uses thereof
CN113558011B (en) * 2021-07-23 2022-05-10 深圳市中医院 Method for establishing sjogren syndrome animal model based on gamma-secretase activated protein gene
CN113893892B (en) * 2021-11-10 2023-02-10 大连海事大学 Nanopore based on microfluidic chip and preparation method thereof
CN114276993A (en) * 2021-11-11 2022-04-05 上海昆盟生物科技有限公司 Method for separating arcuated nucleus neurons of rat hypothalamus
CN114457075B (en) * 2021-12-15 2023-05-02 徐州市中心医院 shRNA (short hairpin ribonucleic acid) and lentiviral vector for knocking down PXYLP1 gene expression, and construction method and application thereof
CN114751990B (en) * 2022-04-01 2023-10-20 中国科学技术大学 Amido homoserine lactone receptor-enhanced green fluorescence fusion protein, preparation method and application thereof
WO2023215805A1 (en) * 2022-05-05 2023-11-09 Alnylam Pharmaceuticals, Inc. ACTIN-BINDING LIM PROTEIN 3 (ABLIM3) iRNA AGENT COMPOSITIONS AND METHODS OF USE THEREOF
CN115083522B (en) * 2022-08-18 2022-10-28 天津诺禾致源生物信息科技有限公司 Method and device for predicting cell types and server

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EP2227568B1 (en) * 2008-01-04 2012-11-14 Centre National de la Recherche Scientifique Molecular in vitro diagnosis of breast cancer
US20110071049A1 (en) * 2008-03-12 2011-03-24 Nathaniel Heintz Methods and compositions for translational profiling and molecular phenotyping
WO2012105854A2 (en) * 2011-01-31 2012-08-09 Curonz Holdings Company Limited Compositions for administration to the eye
EP3031929A1 (en) * 2014-12-11 2016-06-15 Mdc Max-Delbrück-Centrum Für Molekulare Medizin Berlin - Buch Genome architecture mapping

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Publication number Publication date
IL268417A (en) 2019-09-26
JP7229538B2 (en) 2023-03-01
JP2020508652A (en) 2020-03-26
JP2023058610A (en) 2023-04-25
CN110582578B (en) 2024-02-02
EP3580338A1 (en) 2019-12-18
AU2018219308A1 (en) 2019-08-22
CN110582578A (en) 2019-12-17
EP3580338A4 (en) 2021-05-26
CA3052290A1 (en) 2018-08-16
WO2018148501A1 (en) 2018-08-16
MX2019009612A (en) 2019-11-12

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