SG11201810850XA - A recombinant strain of bacillus subtilis, the preparation method thereof and the use therefor - Google Patents

A recombinant strain of bacillus subtilis, the preparation method thereof and the use therefor

Info

Publication number
SG11201810850XA
SG11201810850XA SG11201810850XA SG11201810850XA SG11201810850XA SG 11201810850X A SG11201810850X A SG 11201810850XA SG 11201810850X A SG11201810850X A SG 11201810850XA SG 11201810850X A SG11201810850X A SG 11201810850XA SG 11201810850X A SG11201810850X A SG 11201810850XA
Authority
SG
Singapore
Prior art keywords
bacillus subtilis
recombinant strain
preparation
use therefor
protease genes
Prior art date
Application number
SG11201810850XA
Other languages
English (en)
Inventor
Li Pan
Xin Liu
Bin Wang
Original Assignee
Univ South China Tech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ South China Tech filed Critical Univ South China Tech
Publication of SG11201810850XA publication Critical patent/SG11201810850XA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
SG11201810850XA 2016-06-15 2016-12-06 A recombinant strain of bacillus subtilis, the preparation method thereof and the use therefor SG11201810850XA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201610429216.9A CN106085937B (zh) 2016-06-15 2016-06-15 一种枯草芽孢杆菌重组菌株及其制备方法与应用
PCT/CN2016/108692 WO2017215204A1 (fr) 2016-06-15 2016-12-06 Souche recombinante de bacillus subtilis et procédé de préparation correspondant et son application

Publications (1)

Publication Number Publication Date
SG11201810850XA true SG11201810850XA (en) 2019-01-30

Family

ID=57235389

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201810850XA SG11201810850XA (en) 2016-06-15 2016-12-06 A recombinant strain of bacillus subtilis, the preparation method thereof and the use therefor

Country Status (3)

Country Link
CN (1) CN106085937B (fr)
SG (1) SG11201810850XA (fr)
WO (1) WO2017215204A1 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106085937B (zh) * 2016-06-15 2019-07-16 华南理工大学 一种枯草芽孢杆菌重组菌株及其制备方法与应用
CN108102997A (zh) * 2017-10-25 2018-06-01 江南大学 一种用于普鲁兰酶高效表达和高密度培养的枯草芽孢杆菌
CN108570477B (zh) * 2018-04-18 2020-06-30 横琴仲泰生物医药有限公司 一种碱性蛋白酶基因及其重组枯草芽孢杆菌菌株的构建方法
CN108913706B (zh) * 2018-07-10 2021-04-09 郑州轻工业学院 一种枯草芽孢杆菌甘油激酶突变基因glpK及其应用
CN109554321B (zh) * 2018-12-03 2021-12-31 清华大学 一种高产脂肽的基因工程菌及其应用
CN111349594A (zh) * 2018-12-21 2020-06-30 南京百斯杰生物工程有限公司 一株能够生产谷氨酰胺转氨酶的菌株
CN111349639A (zh) * 2020-01-17 2020-06-30 西宝生物科技（上海）股份有限公司 一种提高纳豆芽孢杆菌高效生物合成维生素k2（mk-7）的方法
CN111471635B (zh) * 2020-04-13 2022-02-15 江南大学 一种提高枯草芽孢杆菌核酸含量的方法
CN112592881B (zh) * 2021-02-25 2021-06-11 中国科学院天津工业生物技术研究所 用于高效外源蛋白表达和高密度培养的工程枯草芽孢杆菌
CN113957028B (zh) * 2021-10-25 2023-08-11 江南大学 一种胞外蛋白酶失活的枯草芽孢杆菌及其构建方法与应用
CN115181714B (zh) * 2022-05-31 2023-08-11 南京农业大学 一种植物免疫诱抗菌及其构建方法和应用
CN116396915B (zh) * 2022-11-18 2023-10-31 西北农林科技大学 一株无特定抗性基因枯草芽孢杆菌及应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4485341B2 (ja) * 2004-12-20 2010-06-23 花王株式会社 組換え微生物
US20080090745A1 (en) * 2006-10-13 2008-04-17 Fox Bryan P Expression of Streptomyces subtilism inhibitor (SSI) proteins in Bacillus and Streptomyces sp.
CN103451224B (zh) * 2013-08-26 2014-12-24 天津大学 枯草芽孢杆菌基因组无痕修饰方法
CN106085937B (zh) * 2016-06-15 2019-07-16 华南理工大学 一种枯草芽孢杆菌重组菌株及其制备方法与应用

Also Published As

Publication number Publication date
CN106085937B (zh) 2019-07-16
CN106085937A (zh) 2016-11-09
WO2017215204A1 (fr) 2017-12-21

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