SG11201808976WA - Method of producing a recombinant protein - Google Patents

Method of producing a recombinant protein

Info

Publication number
SG11201808976WA
SG11201808976WA SG11201808976WA SG11201808976WA SG11201808976WA SG 11201808976W A SG11201808976W A SG 11201808976WA SG 11201808976W A SG11201808976W A SG 11201808976WA SG 11201808976W A SG11201808976W A SG 11201808976WA SG 11201808976W A SG11201808976W A SG 11201808976WA
Authority
SG
Singapore
Prior art keywords
glaxosmithkline
road
international
hertfordshire
recombinant protein
Prior art date
Application number
SG11201808976WA
Other languages
English (en)
Inventor
Robyn Alexandra Emmins
Gary Brian Finka
Michael Hoare
Alan Peter Lewis
William John Kenneth Lewis
Adam Daniel Mcinally
Mark Uden
Ioannis Voulgaris
Original Assignee
Glaxosmithkline Ip Dev Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glaxosmithkline Ip Dev Ltd filed Critical Glaxosmithkline Ip Dev Ltd
Publication of SG11201808976WA publication Critical patent/SG11201808976WA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245Escherichia (G)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503Immunoglobulin superfamily
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K7/00Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04Linear peptides containing only normal peptide links
    • C07K7/06Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00Nucleic acids vectors
    • C12N2800/22Vectors comprising a coding region that has been codon optimised for expression in a respective host

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
SG11201808976WA 2016-05-06 2017-05-04 Method of producing a recombinant protein SG11201808976WA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662332636P 2016-05-06 2016-05-06
US201662338045P 2016-05-18 2016-05-18
PCT/EP2017/060654 WO2017191255A1 (en) 2016-05-06 2017-05-04 Method of producing a recombinant protein

Publications (1)

Publication Number Publication Date
SG11201808976WA true SG11201808976WA (en) 2018-11-29

Family

ID=58765814

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201808976WA SG11201808976WA (en) 2016-05-06 2017-05-04 Method of producing a recombinant protein

Country Status (14)

Country Link
US (1) US11098312B2 (zh)
EP (1) EP3452496A1 (zh)
JP (2) JP2019516371A (zh)
KR (1) KR102504317B1 (zh)
CN (1) CN109071611B (zh)
AU (1) AU2017260277A1 (zh)
BR (1) BR112018072043A2 (zh)
CA (1) CA3023025A1 (zh)
IL (1) IL262544A (zh)
MX (1) MX2018013562A (zh)
RU (1) RU2742006C2 (zh)
SG (1) SG11201808976WA (zh)
TW (1) TWI794170B (zh)
WO (1) WO2017191255A1 (zh)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102094480B1 (ko) 2019-05-27 2020-04-01 (주)엘에스케이화인텍스 재사용 가능한 미세 먼지 항균 마스크
CN110551199B (zh) * 2019-09-26 2022-01-25 杭州纽龙日尚生物制品有限公司 一种提高工程菌分泌表达重组人表皮生长因子的方法
US20230100757A1 (en) * 2021-06-10 2023-03-30 Pfenex Inc. Bacterial hosts for recombinant protein expression

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005538731A (ja) * 2002-09-20 2005-12-22 シーワイエム1ピー エイ/エス 宿主細胞からの組換えポリペプチドの産生を高める方法
GB0426160D0 (en) * 2004-11-29 2004-12-29 Era Plantech S L Production of proteins
AU2007210396B2 (en) 2006-01-31 2011-09-29 Bexcore Inc. Production of a soluble native form of recombinant protein by the signal sequence and secretional enhancer
GB0602173D0 (en) 2006-02-03 2006-03-15 Avecia Ltd Expression system
WO2009021548A1 (en) * 2007-08-10 2009-02-19 Wacker Chemie Ag Expression of full length igg and secretion into the culture medium of prokaryotic cells
RU2488635C1 (ru) * 2012-03-22 2013-07-27 Государственное бюджетное образовательное учреждение высшего профессионального образования Первый Московский государственный медицинский университет им. И.М. Сеченова Министерства здравоохранения и социального развития Российской Федерации (ГБОУ ВПО Первый МГМУ им. И.М. Сеченова Минздравсоцразвития Способ получения рекомбинантного антиангиогенного полипептида
US20140244228A1 (en) * 2012-09-19 2014-08-28 Agency For Science, Technology And Research Codon optimization of a synthetic gene(s) for protein expression

Also Published As

Publication number Publication date
BR112018072043A2 (pt) 2019-02-12
RU2742006C2 (ru) 2021-02-01
CN109071611B (zh) 2022-11-01
IL262544A (en) 2018-12-31
CN109071611A (zh) 2018-12-21
EP3452496A1 (en) 2019-03-13
US11098312B2 (en) 2021-08-24
JP2019516371A (ja) 2019-06-20
MX2018013562A (es) 2019-03-14
CA3023025A1 (en) 2017-11-09
WO2017191255A1 (en) 2017-11-09
JP2022070902A (ja) 2022-05-13
KR102504317B1 (ko) 2023-02-24
RU2018137305A (ru) 2020-06-08
TWI794170B (zh) 2023-03-01
RU2018137305A3 (zh) 2020-06-08
TW201805298A (zh) 2018-02-16
US20190177734A1 (en) 2019-06-13
KR20190003694A (ko) 2019-01-09
AU2017260277A1 (en) 2018-11-22

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