SG11201806916RA - A method for increasing resistant starch and dietary fibre in rice - Google Patents

A method for increasing resistant starch and dietary fibre in rice

Info

Publication number
SG11201806916RA
SG11201806916RA SG11201806916RA SG11201806916RA SG11201806916RA SG 11201806916R A SG11201806916R A SG 11201806916RA SG 11201806916R A SG11201806916R A SG 11201806916RA SG 11201806916R A SG11201806916R A SG 11201806916RA SG 11201806916R A SG11201806916R A SG 11201806916RA
Authority
SG
Singapore
Prior art keywords
international
rice
lotus
starch
dietary fibre
Prior art date
Application number
SG11201806916RA
Inventor
Dr R Bharathi Raja
Original Assignee
Udaya Agro Farm
Green Flora Biosciences Pvt Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Udaya Agro Farm, Green Flora Biosciences Pvt Ltd filed Critical Udaya Agro Farm
Publication of SG11201806916RA publication Critical patent/SG11201806916RA/en

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4636—Oryza sp. [rice]
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
    • A23L29/206—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
    • A23L29/212—Starch; Modified starch; Starch derivatives, e.g. esters or ethers
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10—Cereal-derived products
    • A23L7/198—Dry unshaped finely divided cereal products, not provided for in groups A23L7/117 - A23L7/196 and A23L29/00, e.g. meal, flour, powder, dried cereal creams or extracts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01011—Glycogen(starch) synthase (2.4.1.11)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Nutrition Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2017/141128 Al 24 August 2017 (24.08.2017) WIPO I PCT 111111111111110111011111111111010111110111010111110111011101111101111111111111111111 W O 20 17 / 141128 Al (51) International Patent Classification: C12N 15/82 (2006.01) A23L 29/212 (2016.01) A23L 29/00 (2016.01) (21) International Application Number: PCT/IB2017/050558 (22) International Filing Date: 2 February 2017 (02.02.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 201641005181 15 February 2016 (15.02.2016) IN (71) Applicants: UDAYA AGRO FARM [IN/IN]; 62/61, Bharath Nagar, Kumbakonam 612001 (IN). GREEN FLORA BIOSCIENCES PVT. LTD [IN/IN]; Plot No. 14, Maxworth Nagar, Phase III, Kolapakkam, Chennai 600128 (IN). (72) Inventor: RAJA, Dr R. Bharathi; Number 27, San- jeevirayanpet 4th Street, Tindivanam, Villupuram District, Tamil Nadu, Tindivanam 604001 (IN). (74) Agents: KALIA, Anita et al.; #213, Sobha Aquamarine, Sarjapur Outer Ring Road, Bellandur, Bangalore - Karnataka 560103 (IN). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) = (54) Title: A METHOD FOR INCREASING RESISTANT STARCH AND DIETARY FIBRE IN RICE Sample No. and details of mutations detected % Chain distribution Amylose content % AC Resistant Total Starch % RS Dietary Fibre % TDF Hydrolysis Index % HI Short Chains (DP 6- 12) Medium Chains (DP 13- (DP>19) 18) Long Chains Lotus 1 3 (ss111 - a, sbel and sbellb 2 ) 35.75 43.93 20.32 26.8 9.37 11.12 38.4 Lotus 2 2 (ssl and sbellb 2 ) 25.49 52.06 22.45 25.1 5.12 6.87 48.2 Lotus 3 2 (shell - h i and ss///a) 31.05 46.23 22.71 27.4 7.07 9.74 42.1 Lotus 4 2 (ssl and ss 111a) 42.34 40.75 16.91 29.3 11.92 13.21 33.2 Os 278 Nil 22.19 48.96 28.86 23.4 1.21 1.87 72.6 Figure 1 (57) : The present invention discloses mutations in the starch synthase genes associated with enhanced dietary fibre and res - istant starch levels in the endosperm of a suitable variety of rice. The dietary fiber and resistant starch are enhanced to an extent to significantly reduce the hydrolysis index values of the rice grains to 35-40%. These rice varieties are in great demand for diabetic population and provide a number of other health benefits such as reduced body weight gain, cardiac health and colon health. As this strategy does not involve the use of genetic manipulation technologies, it can be directly employed in the rice breeding programmes without any restrictions.
SG11201806916RA 2016-02-15 2017-02-02 A method for increasing resistant starch and dietary fibre in rice SG11201806916RA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN201641005181 2016-02-15
PCT/IB2017/050558 WO2017141128A1 (en) 2016-02-15 2017-02-02 A method for increasing resistant starch and dietary fibre in rice

Publications (1)

Publication Number Publication Date
SG11201806916RA true SG11201806916RA (en) 2018-09-27

Family

ID=59624816

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201806916RA SG11201806916RA (en) 2016-02-15 2017-02-02 A method for increasing resistant starch and dietary fibre in rice

Country Status (10)

Country Link
US (1) US20190071687A1 (en)
EP (1) EP3430144A4 (en)
JP (1) JP2019509035A (en)
CN (1) CN109689874A (en)
AU (1) AU2017220749A1 (en)
BR (1) BR112018016631A2 (en)
MX (1) MX2018009896A (en)
PH (1) PH12018550136A1 (en)
SG (1) SG11201806916RA (en)
WO (1) WO2017141128A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7239136B2 (en) * 2018-07-25 2023-03-14 公立大学法人秋田県立大学 Rice mutant with high resistant starch content, method for producing rice flour, method for producing resistant starch, method for producing rice gel, method for producing food, and method for producing mutant rice with high resistant starch content
CN115152617B (en) * 2022-07-07 2023-09-05 湖南农业大学 Breeding method and application of high-resistance starch content and high-low temperature resistant indica rice variety

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4703919B2 (en) * 2001-09-12 2011-06-15 独立行政法人科学技術振興機構 Function elucidation of starch synthase type I and new starch production method
CN1886507B (en) * 2003-10-27 2010-12-29 联邦科技产业研究组织 Rice and products thereof having starch with an increased proportion of amylose
KR100877837B1 (en) * 2006-12-18 2009-01-12 경희대학교 산학협력단 Modification of Endosperm Starch Property by Loss of Function of Starch Synthase in Dongjin rice
JP5750635B2 (en) * 2010-07-15 2015-07-22 公立大学法人秋田県立大学 Rice variant, method for producing starch, starch, and method for producing rice variant
CN104017829A (en) * 2011-12-06 2014-09-03 中国科学院上海生命科学研究院 Method for increasing amylose content of plants
CN103146746B (en) * 2013-03-18 2014-10-15 扬州大学 Transgenic rice with excellent eating quality and cultivation method
WO2015004638A2 (en) * 2013-07-12 2015-01-15 Texcity Bio-Sciences Pvt. Ltd Mutations in starch biosynthesis genes leading to high fiber and resistant starch expression in rice endosperm

Also Published As

Publication number Publication date
EP3430144A4 (en) 2019-12-04
PH12018550136A1 (en) 2019-12-11
JP2019509035A (en) 2019-04-04
BR112018016631A2 (en) 2020-11-03
EP3430144A1 (en) 2019-01-23
WO2017141128A1 (en) 2017-08-24
US20190071687A1 (en) 2019-03-07
AU2017220749A1 (en) 2018-10-04
CN109689874A (en) 2019-04-26
MX2018009896A (en) 2019-01-21

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