SG11201707615YA - Methods and compositions for producing hepatocyte-like cells - Google Patents

Methods and compositions for producing hepatocyte-like cells

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Publication number
SG11201707615YA
SG11201707615YA SG11201707615YA SG11201707615YA SG11201707615YA SG 11201707615Y A SG11201707615Y A SG 11201707615YA SG 11201707615Y A SG11201707615Y A SG 11201707615YA SG 11201707615Y A SG11201707615Y A SG 11201707615YA SG 11201707615Y A SG11201707615Y A SG 11201707615YA
Authority
SG
Singapore
Prior art keywords
compositions
cells
methods
producing hepatocyte
hepatocyte
Prior art date
Application number
SG11201707615YA
Other languages
English (en)
Inventor
Gary Peltz
Dan Xu
Original Assignee
Univ Leland Stanford Junior
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Leland Stanford Junior filed Critical Univ Leland Stanford Junior
Publication of SG11201707615YA publication Critical patent/SG11201707615YA/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/407—Liver; Hepatocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C12N2500/62—DMSO
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/12—Hepatocyte growth factor [HGF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/237—Oncostatin M [OSM]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/606—Transcription factors c-Myc
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
    • C12N2506/1384—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells from adipose-derived stem cells [ADSC], from adipose stromal stem cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
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    • C12N2510/00—Genetically modified cells
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
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  • Developmental Biology & Embryology (AREA)
  • Animal Behavior & Ethology (AREA)
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  • Gastroenterology & Hepatology (AREA)
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  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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  • Heart & Thoracic Surgery (AREA)
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SG11201707615YA 2013-09-19 2014-09-17 Methods and compositions for producing hepatocyte-like cells SG11201707615YA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361880056P 2013-09-19 2013-09-19
PCT/US2014/056049 WO2015042125A2 (en) 2013-09-19 2014-09-17 Methods and compositions for producing hepatocyte-like cells

Publications (1)

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SG11201707615YA true SG11201707615YA (en) 2017-10-30

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SG11201707615YA SG11201707615YA (en) 2013-09-19 2014-09-17 Methods and compositions for producing hepatocyte-like cells
SG10201914005TA SG10201914005TA (en) 2013-09-19 2014-09-17 Methods and compositions for producing hepatocyte-like cells
SG10201802231RA SG10201802231RA (en) 2013-09-19 2014-09-17 Methods and compositions for producing hepatocyte-like cells

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SG10201802231RA SG10201802231RA (en) 2013-09-19 2014-09-17 Methods and compositions for producing hepatocyte-like cells

Country Status (8)

Country Link
US (3) US10004767B2 (OSRAM)
EP (2) EP3685884A1 (OSRAM)
JP (3) JP6683615B2 (OSRAM)
CA (1) CA2959117C (OSRAM)
DK (1) DK3046628T3 (OSRAM)
ES (1) ES2767062T3 (OSRAM)
SG (3) SG11201707615YA (OSRAM)
WO (1) WO2015042125A2 (OSRAM)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10767164B2 (en) 2017-03-30 2020-09-08 The Research Foundation For The State University Of New York Microenvironments for self-assembly of islet organoids from stem cells differentiation
WO2019071135A1 (en) * 2017-10-05 2019-04-11 The Johns Hopkins University IMPLANTABLE BIOREACTOR AND METHODS OF MAKING AND USING
CN109749981B (zh) * 2017-11-06 2023-10-10 博品(上海)生物医药科技有限公司 人源脂肪干细胞来源的肝细胞样细胞及其制备方法和应用
US20210322644A1 (en) * 2018-09-20 2021-10-21 Novadip Biosciences Biomaterial comprising adipose-derived stem cells and gelatin and method for producing the same
AU2021235252A1 (en) 2020-03-11 2022-10-06 Bit Bio Limited Method of generating hepatic cells
TW202321440A (zh) 2021-10-07 2023-06-01 美商肝特斯公司 追蹤接受者中之供體細胞之方法
US20230174949A1 (en) * 2021-12-03 2023-06-08 The Regents Of The University Of California Engineered vascularized organoids

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4491632A (en) 1979-10-22 1985-01-01 The Massachusetts General Hospital Process for producing antibodies to hepatitis virus and cell lines therefor
US4444887A (en) 1979-12-10 1984-04-24 Sloan-Kettering Institute Process for making human antibody producing B-lymphocytes
EP0043718B1 (en) 1980-07-07 1984-11-28 National Research Development Corporation Improvements in or relating to cell lines
DE3402927A1 (de) 1984-01-28 1985-08-08 Pfeifer & Langen, 5000 Köln Zellkulturmikrotraeger, verfahren zu seiner herstellung und seine verwendung zum zuechten von verankerungsabhaengigen zellen
US5026650A (en) 1988-06-30 1991-06-25 The United States Of Amercia As Represented By The Administrator Of The National Aeronautics And Space Administration Horizontally rotated cell culture system with a coaxial tubular oxygenator
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5631734A (en) 1994-02-10 1997-05-20 Affymetrix, Inc. Method and apparatus for detection of fluorescently labeled materials
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US6214618B1 (en) 1998-04-07 2001-04-10 Solohill Engineering, Inc. Microcarrier beads having a styrene copolymer core and a covalently linked tri-methylamine exterior
US6712610B2 (en) 1999-04-02 2004-03-30 Forsyth Dental Infirmary For Children Characterization of an antibiotic impregnated delivery system as an intracanal medicament in endodontic therapy and method
PL368119A1 (en) 2001-10-02 2005-03-21 Novo Nordisk Health Care Ag Method for production of recombinant proteins in eukaryote cells
WO2003078588A2 (en) * 2002-03-15 2003-09-25 University Of North Carolina At Chapel Hill Primitive and proximal hepatic stem cells
JP4334358B2 (ja) 2002-03-25 2009-09-30 財団法人 ひろしま産業振興機構 増殖性ヒト肝細胞を認識する抗体と、増殖性ヒト肝細胞および機能性ヒト肝細胞
WO2004056976A2 (en) 2002-12-23 2004-07-08 Fuji Photo Film B.V. Process for coating cell-culture support
US7813977B2 (en) 2003-09-11 2010-10-12 Bgc Partners, Inc. Graphical user interface related to non-standard trading of financial instruments
US7361493B1 (en) 2004-05-26 2008-04-22 The United States Of America As Represented By The Secretary Of The Department Of Veterans Affairs Production of urokinase in a three-dimensional cell culture
JP2008514214A (ja) * 2004-09-29 2008-05-08 セルアーティス アーベー ヒト胚盤胞由来幹細胞（ｈＢＳ）から肝細胞様細胞を発生させる方法
US8592209B2 (en) 2006-01-30 2013-11-26 University Of Virginia Patent Foundation Methods of preparing and characterizing mesenchymal stem cell aggregates and uses thereof
AU2007244965A1 (en) 2006-04-25 2007-11-08 Artecel, Inc. Adipose derived adult stem cells in hepatic regeneration
ES2618787T5 (es) 2006-04-25 2022-10-21 Univ California Administración de factores de crecimiento para el tratamiento de trastornos del SNC
US7989204B2 (en) * 2006-04-28 2011-08-02 Viacyte, Inc. Hepatocyte lineage cells
CN101448527B (zh) * 2006-05-19 2013-12-18 香港大学 细胞-基质微球，制备方法和应用
US8158108B2 (en) 2006-06-28 2012-04-17 S.C. Johnson & Son, Inc. VOC-free compressed gas aerosol compositions
US20080081064A1 (en) 2006-09-28 2008-04-03 Surmodics, Inc. Implantable Medical Device with Apertures for Delivery of Bioactive Agents
EP2155065A4 (en) * 2007-05-14 2014-09-10 Univ Johns Hopkins PROCESS FOR THE IMAGE DISPLAY OF CELLS IN VIVO
AU2008280143B2 (en) * 2007-07-20 2014-04-17 Cellartis Ab A novel population of hepatocytes derived via definitive endoderm (DE-hep) from human blastocysts stem cells
CA2712496C (en) 2008-01-18 2021-01-12 Wei-Shou Hu Stem cell aggregates and methods for making and using
EP2257250A2 (en) 2008-01-29 2010-12-08 Gilbert H. Kliman Drug delivery devices, kits and methods therefor
US20100112031A1 (en) 2008-10-17 2010-05-06 University Of Virginia Patent Foundation Compositions And Methods For Regulating Extracellular Matrix Production In Adipose Derived Cells
US20100098739A1 (en) 2008-10-20 2010-04-22 University Of Virginia Patent Foundation Compositions and methods for modular soft tissue repair
EP2362899A1 (en) 2008-10-31 2011-09-07 Katholieke Universiteit Leuven Optimized methods for differentiation of cells into cells with hepatocyte and hepatocyte progenitor phenotypes, cells produced by the methods, and methods for using the cells
US8895300B2 (en) * 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
ES2642070T3 (es) 2008-11-20 2017-11-15 Janssen Biotech, Inc. Cultivo de células madre pluripotentes en microportadores
KR101017710B1 (ko) * 2009-02-05 2011-02-25 한양대학교 산학협력단 세포 스페로이드 형태의 이식용 성체 줄기세포 집합체 및 그 제조방법
WO2010108126A2 (en) * 2009-03-19 2010-09-23 Fate Therapeutics, Inc. Reprogramming compositions and methods of using the same
JP5792714B2 (ja) 2009-05-28 2015-10-14 コーニング インコーポレイテッド 細胞培養のための膨潤性の合成マイクロキャリア
JP2011013535A (ja) 2009-07-03 2011-01-20 Olympus Corp 光調節装置及び光学系
US20120282228A1 (en) * 2009-07-15 2012-11-08 Vishal Bhasin Method of producing progenitor cells from differentiated cells
CN101914495A (zh) 2010-07-22 2010-12-15 吉林大学 用于体外大量扩增毛囊干细胞的培养方法
ITMI20111096A1 (it) * 2011-06-17 2012-12-18 Filosa Stefania Cellule dell'endoderma da cellule pluripotenti
US9215088B2 (en) 2011-06-29 2015-12-15 Broadcom Corporation Identification of application sessions
JP6124347B2 (ja) * 2011-08-02 2017-05-10 石川　哲也 ＴＧＦ−βシグナリング阻害剤および／またはＹ−２７６３２による肝前駆細胞の誘導方法および製造方法、並びに当該肝前駆細胞から肝細胞の製造方法
EP2554176B1 (en) 2011-08-04 2016-07-27 ETHIANUM Betriebsgesellschaft mbH & Co. KG Means for liver regeneration

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