SG11201700955SA - Mutants of the bacteriophage lambda integrase - Google Patents

Mutants of the bacteriophage lambda integrase

Info

Publication number
SG11201700955SA
SG11201700955SA SG11201700955SA SG11201700955SA SG11201700955SA SG 11201700955S A SG11201700955S A SG 11201700955SA SG 11201700955S A SG11201700955S A SG 11201700955SA SG 11201700955S A SG11201700955S A SG 11201700955SA SG 11201700955S A SG11201700955S A SG 11201700955SA
Authority
SG
Singapore
Prior art keywords
mutants
bacteriophage lambda
lambda integrase
integrase
bacteriophage
Prior art date
Application number
SG11201700955SA
Other languages
English (en)
Inventor
Farid Ghadessy
Jia Wei Siau
Peter Droge
Harshyaa Makhija
Chandra Shree Harsh Vijaya
Sabrina Peter
Original Assignee
Agency Science Tech & Res
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency Science Tech & Res filed Critical Agency Science Tech & Res
Publication of SG11201700955SA publication Critical patent/SG11201700955SA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00Bacteriophages
    • C12N2795/00011Details
    • C12N2795/10011Details dsDNA Bacteriophages
    • C12N2795/10311Siphoviridae
    • C12N2795/10322New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
SG11201700955SA 2014-08-08 2015-08-11 Mutants of the bacteriophage lambda integrase SG11201700955SA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1414130.3A GB201414130D0 (en) 2014-08-08 2014-08-08 Mutants of the bacteriophage lambda integrase
PCT/SG2015/050255 WO2016022075A1 (en) 2014-08-08 2015-08-11 Mutants of the bacteriophage lambda integrase

Publications (1)

Publication Number Publication Date
SG11201700955SA true SG11201700955SA (en) 2017-03-30

Family

ID=51629549

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201700955SA SG11201700955SA (en) 2014-08-08 2015-08-11 Mutants of the bacteriophage lambda integrase
SG10202005089XA SG10202005089XA (en) 2014-08-08 2015-08-11 Mutants of the bacteriophage lambda integrase

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG10202005089XA SG10202005089XA (en) 2014-08-08 2015-08-11 Mutants of the bacteriophage lambda integrase

Country Status (7)

Country Link
US (2) US10344301B2 (enExample)
EP (1) EP3177717A4 (enExample)
JP (1) JP6616822B2 (enExample)
CN (1) CN108064279B (enExample)
GB (1) GB201414130D0 (enExample)
SG (2) SG11201700955SA (enExample)
WO (1) WO2016022075A1 (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201414130D0 (en) * 2014-08-08 2014-09-24 Agency Science Tech & Res Mutants of the bacteriophage lambda integrase
GB201603577D0 (en) * 2016-03-01 2016-04-13 Oxford Genetics Ltd Promoter
US11078493B2 (en) 2016-06-21 2021-08-03 Nanyang Technological University Site-specific DNA recombination
EP3638776B1 (en) 2017-06-14 2025-11-05 Technische Universität Dresden Methods and means for genetic alteration of genomes utilizing designer dna recombining enzymes
CA3149188A1 (en) * 2019-07-29 2021-02-04 The Trustees Of Indiana University Materials and methods for treating friedreich's ataxia
AU2020391489A1 (en) 2019-11-25 2022-06-09 Larimar Therapeutics, Inc. Methods for quantifying frataxin activity
EP4142768A1 (en) 2020-04-30 2023-03-08 Larimar Therapeutics, Inc. Methods for treating myelin associated diseases and mitochondria associated diseases
EP4259183A1 (en) 2020-12-12 2023-10-18 Larimar Therapeutics, Inc. Pharmaceutical composition comprising frataxin fusion protein and methods of use thereof
EP4305177A4 (en) * 2021-03-12 2024-12-25 Nanyang Technological University METHOD FOR PRODUCING SEAMLESS DNA VECTORS
WO2023234866A1 (en) * 2022-05-31 2023-12-07 Nanyang Technological University Method for production of a eukaryotic host cell or cell line for lambda-integrase-mediated recombination
CN118546903A (zh) * 2023-02-24 2024-08-27 尧唐(上海)生物科技有限公司 能介导大片段dna整合到哺乳动物基因组的酶及其应用
WO2024181932A1 (en) * 2023-03-02 2024-09-06 Nanyang Technological University Lambda integrase mutants and methods of their use
WO2025006468A2 (en) 2023-06-26 2025-01-02 University Of Hawaii Evolved integrases and methods of using the same for genome editing

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4489424B2 (ja) * 2001-05-30 2010-06-23 グラクソ グループ リミテッド 染色体に基くプラットホーム
CA2522166C (en) * 2001-05-30 2011-05-03 Chromos Molecular Systems, Inc. Lambda integrase mutein for use in recombination
BRPI0922084B1 (pt) * 2008-11-11 2020-12-29 Danisco Us Inc. variante de subtilisina isolada de uma subtilisina de bacillus e sua composição de limpeza
SG170652A1 (en) 2009-11-06 2011-05-30 Agency Science Tech & Res Muteins of the bacteriophage lambda integrases
GB201414130D0 (en) * 2014-08-08 2014-09-24 Agency Science Tech & Res Mutants of the bacteriophage lambda integrase

Also Published As

Publication number Publication date
US11414681B2 (en) 2022-08-16
US20170327847A1 (en) 2017-11-16
CN108064279A (zh) 2018-05-22
CN108064279B (zh) 2022-03-11
WO2016022075A1 (en) 2016-02-11
EP3177717A4 (en) 2017-12-27
SG10202005089XA (en) 2020-07-29
JP6616822B2 (ja) 2019-12-04
GB201414130D0 (en) 2014-09-24
US20200063165A1 (en) 2020-02-27
EP3177717A1 (en) 2017-06-14
JP2017525355A (ja) 2017-09-07
US10344301B2 (en) 2019-07-09

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