SG11201601798RA - A method to direct differentiation of pluripotent stem cells into functional heart muscle - Google Patents
A method to direct differentiation of pluripotent stem cells into functional heart muscleInfo
- Publication number
- SG11201601798RA SG11201601798RA SG11201601798RA SG11201601798RA SG11201601798RA SG 11201601798R A SG11201601798R A SG 11201601798RA SG 11201601798R A SG11201601798R A SG 11201601798RA SG 11201601798R A SG11201601798R A SG 11201601798RA SG 11201601798R A SG11201601798R A SG 11201601798RA
- Authority
- SG
- Singapore
- Prior art keywords
- stem cells
- pluripotent stem
- heart muscle
- direct differentiation
- functional heart
- Prior art date
Links
- 230000004069 differentiation Effects 0.000 title 1
- 210000004165 myocardium Anatomy 0.000 title 1
- 210000001778 pluripotent stem cell Anatomy 0.000 title 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0657—Cardiomyocytes; Heart cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
- C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/40—Regulators of development
- C12N2501/415—Wnt; Frizzeled
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/72—Transferases [EC 2.]
- C12N2501/727—Kinases (EC 2.7.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Rheumatology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cardiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Materials For Medical Uses (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP13185344 | 2013-09-20 | ||
| PCT/EP2014/069951 WO2015040142A1 (en) | 2013-09-20 | 2014-09-19 | A method to direct differentiation of pluripotent stem cells into functional heart muscle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201601798RA true SG11201601798RA (en) | 2016-04-28 |
Family
ID=49261445
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201601798RA SG11201601798RA (en) | 2013-09-20 | 2014-09-19 | A method to direct differentiation of pluripotent stem cells into functional heart muscle |
| SG10201910753RA SG10201910753RA (en) | 2013-09-20 | 2014-09-19 | A method to direct differentiation of pluripotent stem cells into functional heart muscle |
| SG10201802167TA SG10201802167TA (en) | 2013-09-20 | 2014-09-19 | A method to direct differentiation of pluripotent stem cells into functional heart muscle |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG10201910753RA SG10201910753RA (en) | 2013-09-20 | 2014-09-19 | A method to direct differentiation of pluripotent stem cells into functional heart muscle |
| SG10201802167TA SG10201802167TA (en) | 2013-09-20 | 2014-09-19 | A method to direct differentiation of pluripotent stem cells into functional heart muscle |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US10329532B2 (en) |
| EP (2) | EP3575393A1 (en) |
| JP (2) | JP6681825B2 (en) |
| CN (2) | CN112175900A (en) |
| AU (1) | AU2014323098B2 (en) |
| CA (1) | CA2924816C (en) |
| DK (1) | DK3047019T3 (en) |
| ES (1) | ES2741806T3 (en) |
| SG (3) | SG11201601798RA (en) |
| WO (1) | WO2015040142A1 (en) |
Families Citing this family (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10597633B2 (en) | 2014-05-16 | 2020-03-24 | Koninklijke Nederlandse Akademie Van Wetenschappen | Culture method for organoids |
| HUE057135T2 (en) | 2015-09-01 | 2022-04-28 | Ncardia B V | An in vitro method of differentiating a human pluripotent stem cell population into a cardiomyocyte cell population |
| KR101781693B1 (en) | 2015-09-24 | 2017-09-26 | 서울대학교병원 | Process for differentiation of cardiovascular cells from induced pluripotent stem cells prepared by human circulation multipotent adult stem cells(CiMS) and uses thereof |
| GB201603569D0 (en) * | 2016-03-01 | 2016-04-13 | Koninklijke Nederlandse Akademie Van Wetenschappen | Improved differentiation method |
| WO2017156762A1 (en) * | 2016-03-18 | 2017-09-21 | Institute Of Biophysics, Chinese Academy Of Sciences | A cell culture medium and culture medium supplement |
| CN106244526B (en) * | 2016-08-23 | 2020-09-11 | 北京赛贝生物技术有限公司 | Myocardial differentiation kit and culture method of myocardial cells |
| CN106244527B (en) * | 2016-08-29 | 2019-10-08 | 广东依浦赛生物科技有限公司 | Source of people iPS stem cell in vitro directed differentiation is the kit and method of cardiac muscle cell |
| CA3045182A1 (en) * | 2016-12-04 | 2018-06-07 | University Health Network | Generating atrial and ventricular cardiomyocyte lineages from human pluripotent stem cells |
| GB201621594D0 (en) * | 2016-12-19 | 2017-02-01 | Univ Leuven Kath | Novel cell based methods for healing of bone, cartilage and joint disorders |
| WO2018139548A1 (en) * | 2017-01-26 | 2018-08-02 | 国立大学法人大阪大学 | Medium for inducing differentiation of stem cells into mesodermal cells and method for producing mesodermal cells |
| CN106867961A (en) * | 2017-03-27 | 2017-06-20 | 广州润虹医药科技有限公司 | A kind of induced multi-potent stem cell forms mesoblastemic inducing culture and abductive approach |
| WO2019126315A1 (en) * | 2017-12-19 | 2019-06-27 | The Regents Of The University Of Michigan | Cardiac microtissue and uses thereof |
| CN108456654A (en) * | 2018-02-22 | 2018-08-28 | 上海产业技术研究院 | Serum free culture system for promoting source of human stem cell cardiac muscle cell maturation |
| EP3540046A1 (en) * | 2018-03-13 | 2019-09-18 | Medizinische Hochschule Hannover | Process for producing cardiac organoids |
| US20210284965A1 (en) * | 2018-07-13 | 2021-09-16 | Lothar Germeroth | Non-immunogenic engineered tissue and methods of producing and using the same |
| CN111214703B (en) * | 2018-11-25 | 2021-06-15 | 中国科学院大连化学物理研究所 | iPS-derived myocardial cell composite patch and preparation and application thereof |
| CN109619087A (en) * | 2018-11-27 | 2019-04-16 | 山东奥博森生物药业股份有限公司 | A kind of small intestinal mucosa stem cell cryopreserving liquid and cryopreservation methods |
| EP3927814A1 (en) | 2019-02-21 | 2021-12-29 | Stembiosys, Inc. | Methods for the maturation of cardiomyocytes on amniotic fluid cell-derived ecm, cellular constructs, and uses for cardiotoxicity and proarrhythmic screening of drug compounds |
| WO2020171345A1 (en) * | 2019-02-21 | 2020-08-27 | 고려대학교 산학협력단 | METHOD FOR DIFFERENTIATION OF STEM CELL INTO MATURE CARDIOMYOCYTE ACCORDING TO TYPE BY USING TGFβ1 |
| CN109749986B (en) * | 2019-03-13 | 2021-04-02 | 武汉大学 | Method for obtaining pancreatic precursor cells and pancreatic beta cells by differentiation of human pluripotent stem cells |
| KR20220035429A (en) * | 2019-07-12 | 2022-03-22 | 게놈 바이오로직스 유쥐 | Methods for generating cardiac tissue mimetics |
| CN110511904B (en) * | 2019-08-21 | 2021-03-19 | 云南中科灵长类生物医学重点实验室 | Culture medium, culture method and application of pluripotent stem cells |
| EP3835403A1 (en) | 2019-12-11 | 2021-06-16 | Sartorius Stedim Biotech GmbH | Automated medium exchange strategy for suspension cells |
| KR102291265B1 (en) * | 2020-03-19 | 2021-08-18 | 연세대학교 산학협력단 | Cardiac organoids, method for preparing the same and method for evaluating drug toxicity using the same |
| WO2021197334A1 (en) * | 2020-03-31 | 2021-10-07 | 浙江养生堂天然药物研究所有限公司 | Pharmaceutical combination and use thereof |
| EP3945133A1 (en) | 2020-07-29 | 2022-02-02 | Georg-August-Universität Göttingen | Mass production of human pluripotent stem cell derived cardiac stromal cell |
| EP3988138B1 (en) | 2020-10-22 | 2024-01-17 | Georg-August-Universität Göttingen Stiftung Öffentlichen Rechts, Universitätsmedizin | Multilayer engineered heart muscle |
| EP4281767A1 (en) | 2021-01-21 | 2023-11-29 | Sartorius Stedim Biotech GmbH | Application of permittivity measurement probes in an suspension culture aggregate comprising cell aggregates |
| CN117043313A (en) | 2021-01-21 | 2023-11-10 | 赛多利斯斯泰帝生物技术有限公司 | Method for changing culture medium of culture using rotary filter |
| CN113249310B (en) * | 2021-05-17 | 2022-10-11 | 湖北大学 | Method for induced differentiation of expansion pluripotent stem cells into myocardial cells and application |
| CN113337459B (en) * | 2021-06-02 | 2022-09-06 | 呈诺再生医学科技(珠海横琴新区)有限公司 | Method for improving differentiation efficiency of pluripotent stem cells |
| US12076437B2 (en) | 2021-07-12 | 2024-09-03 | Brown University | Proangiogenic protein cocktails delivered in custom biomaterials to revascularize ischemic tissue |
| US20230147883A1 (en) * | 2021-11-28 | 2023-05-11 | Fatemeh Etezadi | Method for prodcing cardiomyocytes from induced pluripotent stem cells in an integrated process |
| WO2024008773A1 (en) * | 2022-07-05 | 2024-01-11 | Repairon Gmbh | Differentiation of ipscs in bioreactors |
| EP4386384A1 (en) | 2022-12-15 | 2024-06-19 | Georg-August-Universität Göttingen Stiftung Öffentlichen Rechts, Universitätsmedizin | Potency assay for pharmaceutical products |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040014209A1 (en) * | 2002-01-23 | 2004-01-22 | Lassar Andrew B. | Compositions and methods for modulating cell differentiation |
| EP1783206A1 (en) * | 2005-11-08 | 2007-05-09 | Universitätsklinikum Hamburg-Eppendorf | Multiring engineered heart tissue construct |
| JP2008307007A (en) * | 2007-06-15 | 2008-12-25 | Bayer Schering Pharma Ag | Human pluripotent stem cell induced from human tissue-originated undifferentiated stem cell after birth |
| US20100278787A1 (en) * | 2007-07-18 | 2010-11-04 | Cellartis Ab | Cardiomyocyte-like cell clusters derived from hbs cells |
| EP2100954A1 (en) * | 2008-03-10 | 2009-09-16 | Assistance Publique - Hopitaux de Paris | Method for generating primate cardiac progenitor cells for clinical use from primate embryonic stem cells, and their applications |
| CN102165058B (en) * | 2008-07-25 | 2015-07-01 | 佐治亚大学研究基金会 | Compositions for mesoderm derived ISLl1+ multipotent cells (IMPs), epicardial progenitor cells (EPCs) and multipotent CXCR4+CD56+ cells (C56Cs) and methods of use |
| US8765117B2 (en) * | 2009-06-10 | 2014-07-01 | The General Hospital Corporation | Generation of vascularized human heart tissue and uses thereof |
| CN101935635A (en) * | 2010-07-30 | 2011-01-05 | 中国人民解放军第二军医大学 | In vitro amplification method of human marrow pluripotent stem cells and method for directionally differentiating same into dopaminergic neuron |
| US9023340B2 (en) * | 2011-07-02 | 2015-05-05 | Chemregen, Inc. | Wnt inhibitors for human stem cell differentiation |
| CN103987854A (en) | 2011-07-21 | 2014-08-13 | 小利兰·斯坦福大学托管委员会 | Cardiomyocytes from induced pluripotent stem cells from patients and methods of use |
| WO2013056072A1 (en) * | 2011-10-13 | 2013-04-18 | Wisconsin Alumni Research Foundation | Generation of cardiomyocytes from human pluripotent stem cells |
| WO2013063305A2 (en) | 2011-10-25 | 2013-05-02 | Mount Sinai School Of Medicine | Directed cardiomyocyte differentiation of stem cells |
| WO2013111875A1 (en) * | 2012-01-27 | 2013-08-01 | 国立大学法人京都大学 | Method for inducing differentiation of pluripotent stem cell into cardiac muscle |
| DK2840132T3 (en) * | 2013-08-22 | 2017-02-20 | Georg-August-Universität Göttingen Stiftung Öffenlichen Rechts Universitätsmedizin | Method of Manufacturing Constructed Heart Muscle (EHM) |
-
2014
- 2014-09-19 SG SG11201601798RA patent/SG11201601798RA/en unknown
- 2014-09-19 AU AU2014323098A patent/AU2014323098B2/en active Active
- 2014-09-19 JP JP2016515530A patent/JP6681825B2/en active Active
- 2014-09-19 DK DK14767019.4T patent/DK3047019T3/en active
- 2014-09-19 SG SG10201910753RA patent/SG10201910753RA/en unknown
- 2014-09-19 CN CN202010891948.6A patent/CN112175900A/en active Pending
- 2014-09-19 ES ES14767019T patent/ES2741806T3/en active Active
- 2014-09-19 CN CN201480063250.8A patent/CN105849253B/en active Active
- 2014-09-19 CA CA2924816A patent/CA2924816C/en active Active
- 2014-09-19 US US14/917,103 patent/US10329532B2/en active Active
- 2014-09-19 EP EP19183577.6A patent/EP3575393A1/en not_active Withdrawn
- 2014-09-19 EP EP14767019.4A patent/EP3047019B1/en active Active
- 2014-09-19 WO PCT/EP2014/069951 patent/WO2015040142A1/en active Application Filing
- 2014-09-19 SG SG10201802167TA patent/SG10201802167TA/en unknown
-
2018
- 2018-09-21 US US16/138,101 patent/US20190010460A1/en not_active Abandoned
-
2019
- 2019-05-20 US US16/416,580 patent/US20190300858A1/en not_active Abandoned
-
2020
- 2020-03-24 JP JP2020052526A patent/JP7548538B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP7548538B2 (en) | 2024-09-10 |
| EP3047019A1 (en) | 2016-07-27 |
| AU2014323098A1 (en) | 2016-05-05 |
| CN105849253A (en) | 2016-08-10 |
| CN105849253B (en) | 2020-10-09 |
| CA2924816C (en) | 2023-09-05 |
| JP2020115866A (en) | 2020-08-06 |
| WO2015040142A1 (en) | 2015-03-26 |
| US20190300858A1 (en) | 2019-10-03 |
| DK3047019T3 (en) | 2019-08-26 |
| JP2016536975A (en) | 2016-12-01 |
| SG10201910753RA (en) | 2020-01-30 |
| AU2014323098B2 (en) | 2020-09-17 |
| EP3575393A1 (en) | 2019-12-04 |
| US20160215264A1 (en) | 2016-07-28 |
| CN112175900A (en) | 2021-01-05 |
| CA2924816A1 (en) | 2015-03-26 |
| EP3047019B1 (en) | 2019-07-03 |
| US10329532B2 (en) | 2019-06-25 |
| JP6681825B2 (en) | 2020-04-15 |
| SG10201802167TA (en) | 2018-04-27 |
| US20190010460A1 (en) | 2019-01-10 |
| ES2741806T3 (en) | 2020-02-12 |
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