SG11201508835YA - Removal of fragments from a sample containing a target protein using activated carbon - Google Patents

Removal of fragments from a sample containing a target protein using activated carbon

Info

Publication number
SG11201508835YA
SG11201508835YA SG11201508835YA SG11201508835YA SG11201508835YA SG 11201508835Y A SG11201508835Y A SG 11201508835YA SG 11201508835Y A SG11201508835Y A SG 11201508835YA SG 11201508835Y A SG11201508835Y A SG 11201508835YA SG 11201508835Y A SG11201508835Y A SG 11201508835YA
Authority
SG
Singapore
Prior art keywords
fragments
removal
activated carbon
target protein
sample containing
Prior art date
Application number
SG11201508835YA
Other languages
English (en)
Inventor
Mikhail Kozlov
Matthew T Stone
Romas Skudas
Kevin Galipeau
Original Assignee
Merck Patent Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent Gmbh filed Critical Merck Patent Gmbh
Publication of SG11201508835YA publication Critical patent/SG11201508835YA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/265—Adsorption chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D39/00—Filtering material for liquid or gaseous fluids
    • B01D39/14—Other self-supporting filtering material ; Other filtering material
    • B01D39/20—Other self-supporting filtering material ; Other filtering material of inorganic material, e.g. asbestos paper, metallic filtering material of non-woven wires
    • B01D39/2055—Carbonaceous material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
    • B01J20/20—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising free carbon; comprising carbon obtained by carbonising processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282—Porous sorbents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/58—Use in a single column
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/64—In a syringe, pipette, e.g. tip or in a tube, e.g. test-tube or u-shape tube
SG11201508835YA 2013-07-12 2014-04-04 Removal of fragments from a sample containing a target protein using activated carbon SG11201508835YA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361845422P 2013-07-12 2013-07-12
PCT/US2014/032937 WO2015005960A1 (en) 2013-07-12 2014-04-04 Removal of fragments from a sample containing a target protein using activated carbon

Publications (1)

Publication Number Publication Date
SG11201508835YA true SG11201508835YA (en) 2015-11-27

Family

ID=50721903

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201508835YA SG11201508835YA (en) 2013-07-12 2014-04-04 Removal of fragments from a sample containing a target protein using activated carbon

Country Status (8)

Country Link
US (1) US10519194B2 (ko)
EP (1) EP3019262A1 (ko)
JP (1) JP6141524B2 (ko)
KR (1) KR101847004B1 (ko)
CN (1) CN105324166B (ko)
CA (1) CA2910770C (ko)
SG (1) SG11201508835YA (ko)
WO (1) WO2015005960A1 (ko)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9096648B2 (en) * 2011-08-19 2015-08-04 Emd Millipore Corporation Methods of reducing level of one or more impurities in a sample during protein purification
US11014962B2 (en) * 2016-04-01 2021-05-25 UCB Biopharma SRL Method for protein purification
WO2018043645A1 (ja) * 2016-08-31 2018-03-08 協和発酵キリン株式会社 活性炭を用いた蛋白質の精製方法
US11155575B2 (en) 2018-03-21 2021-10-26 Waters Technologies Corporation Non-antibody high-affinity-based sample preparation, sorbent, devices and methods

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CA2271255C (en) * 1996-11-27 2011-06-14 Genentech, Inc. Affinity purification of polypeptide on protein a matrix
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EP1678208B1 (en) 2003-10-27 2013-05-15 Wyeth LLC Removal of high molecular weight aggregates using hydroxyapatite chromatography
US20080058507A1 (en) 2004-02-11 2008-03-06 Hui Liu Method For The Removal Of Aggregate Proteins From Recombinant Samples Using Ion Exchange Chromatography
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US7999085B2 (en) 2007-01-09 2011-08-16 Bio-Rad Laboratories, Inc. Enhanced capacity and purification of protein by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
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SI2300497T1 (sl) 2008-06-24 2013-02-28 Octapharma Ag Postopek za äťiĺˇäťenje koagulacijskega faktorja viii
US20110166332A1 (en) 2008-09-12 2011-07-07 Ge Healthcare Bio-Sciences Ab Enhanced antibody aggregate removal with capto adhere in the presence of protein-excluded zwitterions
EP2350127B1 (en) 2008-10-20 2015-01-28 AbbVie Inc. Isolation and purification of antibodies using protein a affinity chromatography
US20100150942A1 (en) 2008-12-03 2010-06-17 Cantor Thomas L Affinity purified human polyclonal antibodies and methods of making and using them
US8497358B2 (en) 2008-12-19 2013-07-30 Takeda Pharmaceutical Company Limited Antibody purification method
CN104801204B (zh) 2009-02-27 2019-01-01 Emd密理博公司 用于除去蛋白质聚集体的具有磺酸基团的膜
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EP2744518A2 (en) * 2011-08-19 2014-06-25 EMD Millipore Corporation Use of small molecules in methods for purification of biomolecules
US9096648B2 (en) * 2011-08-19 2015-08-04 Emd Millipore Corporation Methods of reducing level of one or more impurities in a sample during protein purification
CN104105703A (zh) * 2012-03-26 2014-10-15 Emd密理博公司 带电的氟碳化合物组合物在纯化生物分子的方法中的用途
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Also Published As

Publication number Publication date
US10519194B2 (en) 2019-12-31
CA2910770A1 (en) 2015-01-15
EP3019262A1 (en) 2016-05-18
US20160090399A1 (en) 2016-03-31
CA2910770C (en) 2018-02-27
JP6141524B2 (ja) 2017-06-07
CN105324166B (zh) 2018-01-19
KR20160005703A (ko) 2016-01-15
JP2016527201A (ja) 2016-09-08
CN105324166A (zh) 2016-02-10
WO2015005960A1 (en) 2015-01-15
KR101847004B1 (ko) 2018-04-10

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