SG11201408353QA - An expression construct for sensing cell density and substrate availability and its use in conversion of hydroxycinnamic acids - Google Patents

An expression construct for sensing cell density and substrate availability and its use in conversion of hydroxycinnamic acids

Info

Publication number
SG11201408353QA
SG11201408353QA SG11201408353QA SG11201408353QA SG11201408353QA SG 11201408353Q A SG11201408353Q A SG 11201408353QA SG 11201408353Q A SG11201408353Q A SG 11201408353QA SG 11201408353Q A SG11201408353Q A SG 11201408353QA SG 11201408353Q A SG11201408353Q A SG 11201408353QA
Authority
SG
Singapore
Prior art keywords
international
nanyang
promoter
enzyme
singapore
Prior art date
Application number
SG11201408353QA
Inventor
Matthew Wook Chang
Tat Ming Samuel Lo
Chueh Loo Poh
Original Assignee
Univ Nanyang Tech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Nanyang Tech filed Critical Univ Nanyang Tech
Publication of SG11201408353QA publication Critical patent/SG11201408353QA/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y602/00—Ligases forming carbon-sulfur bonds (6.2)
    • C12Y602/01—Acid-Thiol Ligases (6.2.1)
    • C12Y602/01012—4-Coumarate-CoA ligase (6.2.1.12)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau „ (10) International Publication Number (43) International Publication Date ^ ^ WO 2013/191651 A1 27 December 2013 (27.12.2013) WIPO I PCT (51) International Patent Classification: C12N15/63 (2006.01) C12N15/70 (2006.01) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: PCT/SG2013/000242 12 June 2013 (12.06.2013) English English (30) Priority Data: 61/661,593 19 June 2012 (19.06.2012) (71) Applicant: NANYANG TECHNOLOGICAL UNIVER­ SITY [SG/SG]; 50 Nanyang Avenue, Singapore 639798 (SG). (72) Inventors: CHANG, Matthew Wook; c/o NANYANG TECHNOLOGICAL UNIVERSITY, 50 Nanyang Avenue, Singapore 639798 (SG). LO, Tat Ming Samuel; c/o NAN­ YANG TECHNOLOGICAL UNIVERSITY, 50 Nanyang Avenue, Singapore 639798 (SG). POH, Chueh Loo; c/o NANYANG TECHNOLOGICAL UNIVERSITY, 50 Nan­ yang Avenue, Singapore 639798 (SG). (74) Agent: VIERING, JENTSCHURA & PARTNER LLP; P.O. Box 1088, Rochor Post Office, Rochor Road, Singa­ pore 911833 (SG). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. Ug (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a)) (54) Title: AN EXPRESSION CONSTRUCT FOR SENSING CELL DENSITY AND SUBSTRATE AVAILABILITY AND ITS USE IN CONVERSION OF HYDROXYCINNAMIC ACIDS Time delay/ sensing cell density Sensing substrate acid) => Product (depends on substrate) o 0 i-H o\ m B i-H o CJ o & (57) Abstract: An Expression system; isolated nucleic acid molecule or host cell comprising: (i) A first gene en­ coding for a first enzyme linked to a first promoter, wherein the first promoter is a time delay promoter; (ii) A second gene encoding for a second enzyme capable of us­ ing the product generated by the first enzyme as a sub­ strate, wherein the second gene is operably linked to a second promoter, wherein the second promoter is indu­ cible by the product generated by the first enzyme; (iii) Optionally, a third gene encoding a transcription factor that represses expression of the second gene in the ab­ sence of the product generated by the first enzyme,
SG11201408353QA 2012-06-19 2013-06-12 An expression construct for sensing cell density and substrate availability and its use in conversion of hydroxycinnamic acids SG11201408353QA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261661593P 2012-06-19 2012-06-19
PCT/SG2013/000242 WO2013191651A1 (en) 2012-06-19 2013-06-12 An expression construct for sensing cell density and substrate availability and its use in conversion of hydroxycinnamic acids

Publications (1)

Publication Number Publication Date
SG11201408353QA true SG11201408353QA (en) 2015-01-29

Family

ID=49769121

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201408353QA SG11201408353QA (en) 2012-06-19 2013-06-12 An expression construct for sensing cell density and substrate availability and its use in conversion of hydroxycinnamic acids
SG10201610621XA SG10201610621XA (en) 2012-06-19 2013-06-12 An Expression Construct For Sensing Cell Density And Substrate Availability And Its Use In Conversion Of Hydroxycinnamic Acids

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG10201610621XA SG10201610621XA (en) 2012-06-19 2013-06-12 An Expression Construct For Sensing Cell Density And Substrate Availability And Its Use In Conversion Of Hydroxycinnamic Acids

Country Status (4)

Country Link
US (1) US10683521B2 (en)
EP (1) EP2877582B1 (en)
SG (2) SG11201408353QA (en)
WO (1) WO2013191651A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10435697B2 (en) 2014-11-03 2019-10-08 Nanyang Technological University Recombinant expression system that senses pathogenic microorganisms
CN110951760B (en) * 2019-12-20 2021-09-03 台州职业技术学院 Protein time-delay expression switch and application thereof in production of glucaric acid

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7112721B2 (en) * 2000-02-08 2006-09-26 Sakata Seed Corporation Methods and constructs for plant transformation
EP2236613B1 (en) * 2004-07-15 2015-05-27 DSM IP Assets B.V. Biochemical synthesis of 1,4-butanediamine
CN101365783B (en) * 2005-11-15 2013-07-17 格利科菲公司 Production of glycoproteins with reduced o-glycosylation
WO2013189878A1 (en) * 2012-06-19 2013-12-27 Dsm Ip Assets B.V. Promoters for expressing a gene in a cell
US9439972B2 (en) * 2012-09-10 2016-09-13 Ad Lunam Labs, Inc. Antifungal serum
US10077475B2 (en) * 2013-01-24 2018-09-18 California Institute Of Technology FRET-based analytes detection and related methods and systems

Also Published As

Publication number Publication date
US10683521B2 (en) 2020-06-16
EP2877582A4 (en) 2016-03-09
EP2877582A1 (en) 2015-06-03
SG10201610621XA (en) 2017-02-27
US20150176039A1 (en) 2015-06-25
EP2877582B1 (en) 2018-12-19
WO2013191651A1 (en) 2013-12-27

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