SG11201407525VA - Low organic extractable depth filter media processed with solvent extraction method - Google Patents

Abstract

(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 12 December 2013 (12.12.2013) WIPOIPCT (10) International Publication Number WO 2013/184937 A1 (51) International Patent Classification: C12M1/12 (2006.01) C12M 3/06 (2006.01) (21) International Application Number: PCT/US2013/044550 (22) International Filing Date: 6 June 2013 (06.06.2013) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 61/656,263 6 June 2012 (06.06.2012) US 61/664,999 27 June 2012 (27.06.2012) US (71) Applicant: EMD MILLIPORE CORPORATION [US/US]; 290 Concord Road, Billerica, MA 01821 (US). (72) Inventors: CHENG, Kwok-Shun; c/o EMD MILLIPORE CORPORATION, 290 Concord Road, Billerica, MA 01821 (US). SINGH, Nripen; c/o EMD MILLIPORE CORPORATION, 290 Concord Road, Billerica, MA 01821 (US). (74) Agents: HUBBARD, J., Dana et al.; EMD MILLIPORE CORPORATION, 290 Concord Road, Billerica, MA 01821 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, [Continued on next page] (54) Title: LOW ORGANIC EXTRACTABLE DEPTH FILTER MEDIA PROCESSED WITH SOLVENT EXTRACTION METH­ OD Polymer Floccuiants Ch8micai Feed Treatments 100 Mm 100 (jm 50 jjm 50 pm iti yn Celiurass CE25 Figure 1A Polymer Fioccuiants .Cellulose 0=25 Oiato'iTiaceojs _rarfh DE40_ - Figure JB Chemical Feed Treatments 200 (jm ^ 50 |jm 200 Mm ^ 50 jjm 100 Mm J 25 |jm 100 jm 25 Mm ____ It. jJi' sc- «rr. 8 M*n ^ OHulose CF25_ Dlatomi'ceous - ,Ea1h DEI40. - Figure 1C Figure ID (57) Abstract: Provided is a primary clarification depth filtration process of cell-culture feeds, including chemically treated flocculated feeds, containing target biomolecules of interest such as mAbs, mammalian cell cultures, or bacterial cell cultures, utilizing a primary clarification depth filtration device containing media with significantly a lower flushing requirements, res­ ulting in lower levels of organic extractables released after media flushing, and increased throughput for the pre-treated feed streams, without the use of a primary clarification centrifu- gation step or primary clarification tangential flow microfiltration step. The primary clarifica­ tion depth filtration device used in the primary clarification of fluid cell culture feeds, includ - ing chemically treated flocculated feeds containing flocculated cellular debris and/or colloidal particulates having a particle size distribution of about 0.5 (xm to 200 um, contains porous a depth filter media having porous layers of varying pore ratings, and achieves the desired level, of total organic extractables (1-3 ppm) measured in the feed filtered through the media with, significantly lower flushing requirements. Kits and methods of using and making the same are also provided. Polymer Floccuiants Chemical Feed Treatments l> m o\ 00 i-H en i-H o CJ o & Figure IE Figure I F WO 2013/184937 A11 lllll llllllll II llllll III lllll lllll lllll III III III lllll lllll lllll lllll llll lllllll llll llll llll MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h))