SG11201405669XA - Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase - Google Patents
Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymeraseInfo
- Publication number
- SG11201405669XA SG11201405669XA SG11201405669XA SG11201405669XA SG11201405669XA SG 11201405669X A SG11201405669X A SG 11201405669XA SG 11201405669X A SG11201405669X A SG 11201405669XA SG 11201405669X A SG11201405669X A SG 11201405669XA SG 11201405669X A SG11201405669X A SG 11201405669XA
- Authority
- SG
- Singapore
- Prior art keywords
- compositions
- methods
- nucleic acid
- size
- acid polymerase
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/131—Terminal transferase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/121—Modifications characterised by incorporating both deoxyribonucleotides and ribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/173—Modifications characterised by incorporating a polynucleotide run, e.g. polyAs, polyTs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/204—Modifications characterised by specific length of the oligonucleotides
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261610296P | 2012-03-13 | 2012-03-13 | |
US201261613784P | 2012-03-21 | 2012-03-21 | |
PCT/US2013/031104 WO2013138536A1 (fr) | 2012-03-13 | 2013-03-13 | Procédés et compositions pour l'extension homopolymère à taille régulée de polynucléotides de substrat par une polymérase d'acide nucléique |
Publications (1)
Publication Number | Publication Date |
---|---|
SG11201405669XA true SG11201405669XA (en) | 2014-10-30 |
Family
ID=49161798
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11201405669XA SG11201405669XA (en) | 2012-03-13 | 2013-03-13 | Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase |
Country Status (10)
Country | Link |
---|---|
US (4) | US9896709B2 (fr) |
EP (1) | EP2825672B1 (fr) |
JP (2) | JP6509723B2 (fr) |
CN (2) | CN108300765B (fr) |
AU (1) | AU2013232131B2 (fr) |
CA (1) | CA2866625C (fr) |
HK (1) | HK1206395A1 (fr) |
LT (1) | LT2825672T (fr) |
SG (1) | SG11201405669XA (fr) |
WO (1) | WO2013138536A1 (fr) |
Families Citing this family (40)
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US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
SI2556171T1 (sl) | 2010-04-05 | 2016-03-31 | Prognosys Biosciences, Inc. | Prostorsko kodirane biološke analize |
US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
EP3150750B1 (fr) | 2011-04-08 | 2018-12-26 | Prognosys Biosciences, Inc. | Constructions peptidiques et systèmes d'essai |
GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
US9896709B2 (en) * | 2012-03-13 | 2018-02-20 | Swift Biosciences, Inc. | Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase |
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EP3736573A1 (fr) | 2013-03-15 | 2020-11-11 | Prognosys Biosciences, Inc. | Procédés de détection de liaison peptide/mhc/tcr |
DK3013983T3 (da) | 2013-06-25 | 2023-03-06 | Prognosys Biosciences Inc | Spatialt kodede biologiske assays ved brug af en mikrofluidisk anordning |
SG11201601890UA (en) * | 2013-09-13 | 2016-04-28 | Hoffmann La Roche | Application of oligo-dt molecules to avoid generation of high molecular pcr products induced by polya-carrier |
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WO2015070037A2 (fr) | 2013-11-08 | 2015-05-14 | Prognosys Biosciences, Inc. | Conjugués de polynucléotides et procédés de détection d'analytes |
WO2015117040A1 (fr) | 2014-01-31 | 2015-08-06 | Swift Biosciences, Inc. | Procédés améliorés pour traiter des substats d'adn |
WO2015134552A1 (fr) * | 2014-03-03 | 2015-09-11 | Swift Biosciences, Inc. | Ligature d'adaptateur améliorée |
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FR3025201B1 (fr) | 2014-09-02 | 2018-10-12 | Dna Script | Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques |
CA2964799A1 (fr) | 2014-10-17 | 2016-04-21 | Illumina Cambridge Limited | Transposition conservant la contiguite |
CN107969137B (zh) | 2014-10-17 | 2021-10-26 | 伊卢米纳剑桥有限公司 | 接近性保留性转座 |
CN107532207B (zh) | 2015-04-10 | 2021-05-07 | 空间转录公司 | 生物样本的空间区别、多重核酸分析 |
CN107922970B (zh) | 2015-08-06 | 2021-09-28 | 豪夫迈·罗氏有限公司 | 通过单探针引物延伸的靶标富集 |
WO2017032808A1 (fr) * | 2015-08-24 | 2017-03-02 | Qiagen Gmbh | Procédé pour générer une bibliothèque de séquençage d'arn |
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AU2017324946C1 (en) * | 2016-09-06 | 2022-06-09 | Integrated Dna Technologies, Inc. | Normalization of NGS library concentration |
CA3059370C (fr) | 2017-04-12 | 2022-05-10 | Karius, Inc. | Methodes d'analyse simultanee de l'adn et de l'arn dans des echantillonsmelanges |
US11390856B2 (en) | 2017-08-07 | 2022-07-19 | Dna Script | Variants of family a DNA polymerase and uses thereof |
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WO2019090482A1 (fr) * | 2017-11-07 | 2019-05-16 | 北京优乐复生科技有限责任公司 | Procédé de construction de bibliothèque de séquençage à haut rendement de seconde génération |
AU2019205606A1 (en) | 2018-01-08 | 2020-07-30 | Centre National De La Recherche Scientifique | Variants of Terminal deoxynucleotidyl Transferase and uses thereof |
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- 2013-03-13 US US14/384,113 patent/US9896709B2/en active Active
- 2013-03-13 EP EP13761457.4A patent/EP2825672B1/fr active Active
- 2013-03-13 WO PCT/US2013/031104 patent/WO2013138536A1/fr active Application Filing
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- 2013-03-13 CA CA2866625A patent/CA2866625C/fr active Active
- 2013-03-13 CN CN201810004450.6A patent/CN108300765B/zh active Active
- 2013-03-13 LT LTEP13761457.4T patent/LT2825672T/lt unknown
- 2013-03-13 CN CN201380014044.3A patent/CN104395480B/zh active Active
- 2013-03-13 AU AU2013232131A patent/AU2013232131B2/en active Active
- 2013-03-13 SG SG11201405669XA patent/SG11201405669XA/en unknown
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Also Published As
Publication number | Publication date |
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US9896709B2 (en) | 2018-02-20 |
US20150087027A1 (en) | 2015-03-26 |
EP2825672A4 (fr) | 2015-11-18 |
JP6509723B2 (ja) | 2019-05-08 |
US10731194B2 (en) | 2020-08-04 |
JP2018130124A (ja) | 2018-08-23 |
WO2013138536A1 (fr) | 2013-09-19 |
HK1206395A1 (en) | 2016-01-08 |
LT2825672T (lt) | 2019-06-10 |
AU2013232131A1 (en) | 2014-09-18 |
CN108300765A (zh) | 2018-07-20 |
US20180223321A1 (en) | 2018-08-09 |
AU2013232131B2 (en) | 2018-09-20 |
EP2825672B1 (fr) | 2019-02-13 |
CA2866625C (fr) | 2020-12-08 |
CN104395480A (zh) | 2015-03-04 |
EP2825672A1 (fr) | 2015-01-21 |
JP2015510766A (ja) | 2015-04-13 |
CN108300765B (zh) | 2022-01-11 |
CN104395480B (zh) | 2018-01-30 |
CA2866625A1 (fr) | 2013-09-19 |
US20200392551A1 (en) | 2020-12-17 |
US20220186273A1 (en) | 2022-06-16 |
US11118207B2 (en) | 2021-09-14 |
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