SG10201906653YA - Single cell rna and mutational analysis pcr (scrm-pcr): a method for simultaneous analysis of dna and rna at the single-cell level - Google Patents
Single cell rna and mutational analysis pcr (scrm-pcr): a method for simultaneous analysis of dna and rna at the single-cell levelInfo
- Publication number
- SG10201906653YA SG10201906653YA SG10201906653YA SG10201906653YA SG10201906653YA SG 10201906653Y A SG10201906653Y A SG 10201906653YA SG 10201906653Y A SG10201906653Y A SG 10201906653YA SG 10201906653Y A SG10201906653Y A SG 10201906653YA SG 10201906653Y A SG10201906653Y A SG 10201906653YA
- Authority
- SG
- Singapore
- Prior art keywords
- rna
- pcr
- dna
- region
- analysis
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2539/00—Reactions characterised by analysis of gene expression or genome comparison
- C12Q2539/10—The purpose being sequence identification by analysis of gene expression or genome comparison characterised by
- C12Q2539/103—Serial analysis of gene expression [SAGE]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2539/00—Reactions characterised by analysis of gene expression or genome comparison
- C12Q2539/10—The purpose being sequence identification by analysis of gene expression or genome comparison characterised by
- C12Q2539/105—Involving introns, exons, or splice junctions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2549/00—Reactions characterised by the features used to influence the efficiency or specificity
- C12Q2549/10—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals
- C12Q2549/119—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals using nested primers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SG10201500472R | 2015-01-21 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG10201906653YA true SG10201906653YA (en) | 2019-09-27 |
Family
ID=56417482
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG10201906653YA SG10201906653YA (en) | 2015-01-21 | 2016-01-21 | Single cell rna and mutational analysis pcr (scrm-pcr): a method for simultaneous analysis of dna and rna at the single-cell level |
| SG11201704893YA SG11201704893YA (en) | 2015-01-21 | 2016-01-21 | Single cell rna and mutational analysis pcr (scrm-pcr): a method for simultaneous analysis of dna and rna at the single-cell level |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201704893YA SG11201704893YA (en) | 2015-01-21 | 2016-01-21 | Single cell rna and mutational analysis pcr (scrm-pcr): a method for simultaneous analysis of dna and rna at the single-cell level |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US11186865B2 (fr) |
| EP (1) | EP3253888B1 (fr) |
| CN (1) | CN107208154A (fr) |
| SG (2) | SG10201906653YA (fr) |
| WO (1) | WO2016118085A1 (fr) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018162538A1 (fr) * | 2017-03-08 | 2018-09-13 | F. Hoffmann-La Roche Ag | Enrichissement d'extension d'amorce cible et améliorations à celui-ci comprenant l'enrichissement simultané d'adn et d'arn |
| CN109971832A (zh) * | 2017-12-28 | 2019-07-05 | 浙江数问生物技术有限公司 | 一种检测基因突变的试剂盒、方法及其用途 |
| CN110938680A (zh) * | 2019-12-25 | 2020-03-31 | 江苏为真生物医药技术股份有限公司 | Dna及rna同时检测的基因变异位点检测方法 |
| WO2023212223A1 (fr) * | 2022-04-28 | 2023-11-02 | BioSkryb Genomics, Inc. | Multiomique à cellule unique |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07501712A (ja) * | 1992-09-29 | 1995-02-23 | シーダーズ−サイナイ・メディカル・センター | 微量ｒｎａまはたｒｎａおよびｄｎａ検出用の一段ｒｎａおよび結合一段ｒｎａおよびｄｎａポリメラーゼ・チェイン・リアクション |
| US20030082616A1 (en) | 1999-10-15 | 2003-05-01 | Hitachi, Ltd. | Apparatus and method for analyzing nucleic acids and related genetic abnormality |
| WO2003082616A1 (fr) | 2002-04-02 | 2003-10-09 | Youn Sang Choi | Dispositif flottant gonflable escamotable blinde |
| EP2201134B1 (fr) | 2007-09-19 | 2018-11-21 | The Research Foundation of State University of New York | Signatures de l'expression génique dans des échantillons de cellules tumorales enrichis |
| US20090191535A1 (en) | 2007-12-22 | 2009-07-30 | Mark Carle Connelly | Method of assessing metastatic carcinomas from circulating endothelial cells and disseminated tumor cells |
| FR2926091B1 (fr) | 2008-01-09 | 2014-09-05 | Metagenex | Procede et dispositif pour isoler et cultiver des cellules vivantes sur un filtre. |
| WO2013078409A2 (fr) | 2011-11-21 | 2013-05-30 | Creatv Microtech, Inc. | Dispositifs polymères de microfiltration, leurs procédés de fabrication et leurs utilisations |
| US20140315295A1 (en) | 2013-03-15 | 2014-10-23 | Creatv Microtech, Inc. | Polymer microfilters, devices comprising the same, methods of manufacturing the same, and uses thereof |
| WO2011139233A1 (fr) | 2010-05-04 | 2011-11-10 | Agency For Science, Technology And Research | Microtamis pour la filtration de cellules et de particules |
| CN102304581B (zh) | 2011-09-01 | 2013-10-30 | 广州好芝生物科技有限公司 | 用于检测kras基因突变的试剂盒及其检测方法 |
| CA2881783A1 (fr) * | 2012-08-13 | 2014-02-20 | The Regents Of The University Of California | Procedes et systemes de detection de composants biologiques |
| CN104141013B (zh) | 2014-08-08 | 2016-08-24 | 中国人民解放军总医院第一附属医院 | 一种结肠癌诊断试剂盒及其制备方法 |
-
2016
- 2016-01-21 US US15/541,295 patent/US11186865B2/en active Active
- 2016-01-21 EP EP16740482.1A patent/EP3253888B1/fr active Active
- 2016-01-21 SG SG10201906653YA patent/SG10201906653YA/en unknown
- 2016-01-21 SG SG11201704893YA patent/SG11201704893YA/en unknown
- 2016-01-21 WO PCT/SG2016/050026 patent/WO2016118085A1/fr active Application Filing
- 2016-01-21 CN CN201680005645.1A patent/CN107208154A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN107208154A (zh) | 2017-09-26 |
| SG11201704893YA (en) | 2017-07-28 |
| WO2016118085A1 (fr) | 2016-07-28 |
| EP3253888B1 (fr) | 2021-03-03 |
| EP3253888A1 (fr) | 2017-12-13 |
| US11186865B2 (en) | 2021-11-30 |
| US20190119721A1 (en) | 2019-04-25 |
| EP3253888A4 (fr) | 2018-06-20 |
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