SG10201704868RA - Methods For Cloning And Manipulating Genomes - Google Patents

Methods For Cloning And Manipulating Genomes

Info

Publication number
SG10201704868RA
SG10201704868RA SG10201704868RA SG10201704868RA SG10201704868RA SG 10201704868R A SG10201704868R A SG 10201704868RA SG 10201704868R A SG10201704868R A SG 10201704868RA SG 10201704868R A SG10201704868R A SG 10201704868RA SG 10201704868R A SG10201704868R A SG 10201704868RA
Authority
SG
Singapore
Prior art keywords
cloning
methods
genomes
manipulating
manipulating genomes
Prior art date
Application number
SG10201704868RA
Other languages
English (en)
Inventor
Gwynedd A Benders
John I Glass
Clyde A Hutchison
Carole Lartigue
Sanjay Vashee
Mikkel A Algire
Hamilton O Smith
Charles E Merryman
Daniel G Gibson
J Craig Venter
Ray-Yuan Chuang
Vladimir N Noskov
Original Assignee
Synthetic Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=42145125&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=SG10201704868R(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Synthetic Genomics Inc filed Critical Synthetic Genomics Inc
Publication of SG10201704868RA publication Critical patent/SG10201704868RA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
SG10201704868RA 2009-03-06 2010-03-05 Methods For Cloning And Manipulating Genomes SG10201704868RA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US15832009P 2009-03-06 2009-03-06

Publications (1)

Publication Number Publication Date
SG10201704868RA true SG10201704868RA (en) 2017-07-28

Family

ID=42145125

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201400436PA SG10201400436PA (en) 2009-03-06 2010-03-05 Methods For Cloning And Manipulating Genomes
SG10201704868RA SG10201704868RA (en) 2009-03-06 2010-03-05 Methods For Cloning And Manipulating Genomes

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG10201400436PA SG10201400436PA (en) 2009-03-06 2010-03-05 Methods For Cloning And Manipulating Genomes

Country Status (10)

Country Link
US (2) US9273310B2 (zh)
EP (2) EP3249045A1 (zh)
JP (4) JP5713925B2 (zh)
CN (2) CN105274004B (zh)
AU (1) AU2010221172B2 (zh)
CA (1) CA2754212C (zh)
DK (1) DK2403944T3 (zh)
IL (2) IL214828A0 (zh)
SG (2) SG10201400436PA (zh)
WO (1) WO2010102257A2 (zh)

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US20110269119A1 (en) 2009-10-30 2011-11-03 Synthetic Genomics, Inc. Encoding text into nucleic acid sequences
EP2542676A1 (en) * 2010-03-05 2013-01-09 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
US7919605B1 (en) * 2010-08-30 2011-04-05 Amyris, Inc. Nucleic acids, compositions and methods for the excision of target nucleic acids
WO2013066438A2 (en) 2011-07-22 2013-05-10 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
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JP2016517687A (ja) 2013-03-27 2016-06-20 サンプル テクノロジーズ，インコーポレイティド 組換えファージ及び細菌検出方法
WO2015017733A1 (en) 2013-07-31 2015-02-05 Massachusetts Institute Of Technology Methods and apparatus for transplantation of nucleic acid molecules
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
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US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
JP2016536021A (ja) 2013-11-07 2016-11-24 エディタス・メディシン，インコーポレイテッド ＣＲＩＳＰＲ関連方法および支配ｇＲＮＡのある組成物
NL2011912C2 (en) * 2013-12-06 2015-06-09 Univ Delft Tech Novel genome alteration system for microorganisms.
US9068179B1 (en) 2013-12-12 2015-06-30 President And Fellows Of Harvard College Methods for correcting presenilin point mutations
CN106029886B (zh) * 2013-12-19 2021-02-05 阿迈瑞斯公司 基因组整合的方法
US12054771B2 (en) * 2014-02-18 2024-08-06 Bionano Genomics, Inc. Methods of determining nucleic acid structural information
WO2016022363A2 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
US9963687B2 (en) 2014-08-27 2018-05-08 New England Biolabs, Inc. Fusion polymerase and method for using the same
JP6594955B2 (ja) 2014-08-27 2019-10-23 ニユー・イングランド・バイオレイブス・インコーポレイテツド シントン形成
US11845928B2 (en) 2015-05-04 2023-12-19 Tsinghua University Methods and kits for fragmenting DNA
IL294014B2 (en) 2015-10-23 2024-07-01 Harvard College Nucleobase editors and their uses
US11085037B2 (en) * 2016-03-23 2021-08-10 Codex Dna, Inc. Generation of synthetic genomes
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WO2018071868A1 (en) 2016-10-14 2018-04-19 President And Fellows Of Harvard College Aav delivery of nucleobase editors
US10745677B2 (en) 2016-12-23 2020-08-18 President And Fellows Of Harvard College Editing of CCR5 receptor gene to protect against HIV infection
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
JP2020510439A (ja) 2017-03-10 2020-04-09 プレジデント アンド フェローズ オブ ハーバード カレッジ シトシンからグアニンへの塩基編集因子
IL269458B2 (en) 2017-03-23 2024-02-01 Harvard College Nucleic base editors that include nucleic acid programmable DNA binding proteins
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
EP3655528A1 (en) * 2017-07-21 2020-05-27 Basf Se Method of transforming bacterial cells
JP2020534795A (ja) 2017-07-28 2020-12-03 プレジデント アンド フェローズ オブ ハーバード カレッジ ファージによって支援される連続的進化（ｐａｃｅ）を用いて塩基編集因子を進化させるための方法および組成物
US11319532B2 (en) 2017-08-30 2022-05-03 President And Fellows Of Harvard College High efficiency base editors comprising Gam
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CN108949743A (zh) * 2018-06-15 2018-12-07 翁炳焕 一种全基因组杂交克隆方法
DE112020001342T5 (de) 2019-03-19 2022-01-13 President and Fellows of Harvard College Verfahren und Zusammensetzungen zum Editing von Nukleotidsequenzen
ES2923629T3 (es) * 2019-04-04 2022-09-29 Regeneron Pharma Métodos para la introducción sin cicatrices de modificaciones dirigidas en vectores de direccionamiento
CN111154820B (zh) * 2020-01-13 2021-12-21 上海韦翰斯生物医药科技有限公司 一种降低核酸扩增复制滑移率的方法
DE112021002672T5 (de) 2020-05-08 2023-04-13 President And Fellows Of Harvard College Vefahren und zusammensetzungen zum gleichzeitigen editieren beider stränge einer doppelsträngigen nukleotid-zielsequenz
CN112626102B (zh) * 2020-10-15 2023-01-20 天津科技大学 毕赤酵母定点dsb系统及应用
WO2023060351A1 (en) * 2021-10-13 2023-04-20 The University Of Western Ontario Genetic engineering with deinococcus radiodurans
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Also Published As

Publication number Publication date
JP2015128444A (ja) 2015-07-16
CN102421897A (zh) 2012-04-18
JP2017140055A (ja) 2017-08-17
DK2403944T3 (da) 2019-05-27
JP6467460B2 (ja) 2019-02-13
US20110053272A1 (en) 2011-03-03
EP2403944A2 (en) 2012-01-11
US20160177338A1 (en) 2016-06-23
JP2019017391A (ja) 2019-02-07
CN105274004A (zh) 2016-01-27
IL214828A0 (en) 2011-11-30
JP5713925B2 (ja) 2015-05-07
US9273310B2 (en) 2016-03-01
EP3249045A1 (en) 2017-11-29
SG10201400436PA (en) 2014-06-27
WO2010102257A3 (en) 2011-01-20
EP2403944B1 (en) 2017-06-28
WO2010102257A2 (en) 2010-09-10
AU2010221172B2 (en) 2015-06-04
CA2754212A1 (en) 2010-09-10
JP6637140B2 (ja) 2020-01-29
CN105274004B (zh) 2019-08-09
AU2010221172A1 (en) 2011-09-22
IL242303A (en) 2017-06-29
JP2012519493A (ja) 2012-08-30
CA2754212C (en) 2016-08-02
CN102421897B (zh) 2015-12-16

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