SE8803528D0 - CLONING AND EXPRESSION OF TRANSFORMING GROWTH FACTOR BETA2 - Google Patents

CLONING AND EXPRESSION OF TRANSFORMING GROWTH FACTOR BETA2

Info

Publication number
SE8803528D0
SE8803528D0 SE8803528A SE8803528A SE8803528D0 SE 8803528 D0 SE8803528 D0 SE 8803528D0 SE 8803528 A SE8803528 A SE 8803528A SE 8803528 A SE8803528 A SE 8803528A SE 8803528 D0 SE8803528 D0 SE 8803528D0
Authority
SE
Sweden
Prior art keywords
beta
tgf
mature
cells
tamoxifen
Prior art date
Application number
SE8803528A
Other languages
Swedish (sv)
Other versions
SE8803528L (en
Inventor
A F Purchio
L Madisen
N Webb
Original Assignee
Oncogen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oncogen filed Critical Oncogen
Publication of SE8803528D0 publication Critical patent/SE8803528D0/en
Publication of SE8803528L publication Critical patent/SE8803528L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/495—Transforming growth factor [TGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity

Abstract

cDNA clones coding for TGF- beta 2 which are used to construct expression vectors capable of directing the high-level expression of mature, biologically active TGF- beta 2 in transfected Chinese Hamster Ovary cells (CHO cells) are described. The TGF- beta 2 produced and secreted by CHO transfectants has a specific activity estimated to be about half that of recombinant TGF- beta 1. The clones were isolated from a tamoxifen-treated human prostatic adenocarcinoma cell line (PC-3) using oligonucleotide probes based on the partial amino acid sequence of purified TGF- beta 2 from an African green monkey cell line, BSC-40. The cDNA sequences predict that TGF- beta 2 is synthesized as polypeptide precursor forms from which the mature 112 amino acid TGF- beta 2 subunit is derived by proteolytic cleavage. The proteins coded for by the human TGF- beta 1 and TGF- beta 2 cDNAs show an overall homology of 41%. The mature and amino- terminal precursor regions show 71% and 31% homology respectively. Northern blot analysis identified TGF- beta 2 transcripts of 4.1 kb, 5.1 kb, and 6.5 kb using mRNA from several different sources. Analysis of polyadenylated RNA from tamoxifen-treated PC-3 cells showed that these cells contain higher amounts of TGF- beta 1 transcripts than TGF- beta 2, although they produce more TGF- beta 2 protein than TDF- beta 1. This suggests that there is a post-transcriptional level of regulation for the production of these proteins.
SE8803528A 1987-10-06 1988-10-05 CLONING AND EXPRESSION OF TRANSFORMATION GROWTH FACTOR BETA2 SE8803528L (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10675287A 1987-10-06 1987-10-06
US14826788A 1988-01-25 1988-01-25
US23406588A 1988-08-18 1988-08-18

Publications (2)

Publication Number Publication Date
SE8803528D0 true SE8803528D0 (en) 1988-10-05
SE8803528L SE8803528L (en) 1989-04-07

Family

ID=27380183

Family Applications (1)

Application Number Title Priority Date Filing Date
SE8803528A SE8803528L (en) 1987-10-06 1988-10-05 CLONING AND EXPRESSION OF TRANSFORMATION GROWTH FACTOR BETA2

Country Status (22)

Country Link
JP (1) JPH02444A (en)
KR (1) KR970001236B1 (en)
CN (1) CN1035845A (en)
AT (1) AT398433B (en)
BE (1) BE1002693A5 (en)
CH (1) CH680002A5 (en)
DE (1) DE3833897C2 (en)
DK (1) DK556388A (en)
ES (1) ES2012556A6 (en)
FI (1) FI884546A (en)
FR (1) FR2621324B1 (en)
GB (1) GB2210620B8 (en)
GR (1) GR1000492B (en)
IE (1) IE60918B1 (en)
IL (1) IL87877A0 (en)
IT (1) IT1227957B (en)
MY (1) MY103779A (en)
NL (1) NL8802442A (en)
NO (1) NO884429L (en)
NZ (1) NZ226486A (en)
PT (1) PT88670B (en)
SE (1) SE8803528L (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5221620A (en) * 1987-10-06 1993-06-22 Oncogen Cloning and expression of transforming growth factor β2
US5445941A (en) * 1993-06-21 1995-08-29 Eli Lilly And Company Method for screening anti-osteoporosis agents
CN100389120C (en) * 2005-04-12 2008-05-21 中国人民解放军第三军医大学野战外科研究所 Sealing TGF-betal antisensedingonucleotides and its use in preparing medicine for treating fibrillation related disease

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE128715T1 (en) * 1984-07-16 1995-10-15 Celtrix Pharma POLYPEPTIDE-INDUCING FACTORS IN BONE AND CARTILAGE.
IL78197A (en) * 1985-03-22 1991-07-18 Genentech Inc Nucleic acid encoding tgf-beta and its uses
NZ222569A (en) * 1986-11-17 1990-08-28 Sandoz Ltd Production of glioblastoma-derived t cell suppressor factor (g-tsf) using recombinant techniques
US4931548A (en) * 1987-01-30 1990-06-05 Techne Corporation Heterodimer form of transforming growth factor-beta
AU1340688A (en) * 1987-01-30 1988-08-24 Techne Corporation Transforming growth factor-beta
AU1539188A (en) * 1987-05-04 1988-11-10 Bristol-Myers Squibb Company TGF-B2 and novel compositions having anti-neoplastic activity

Also Published As

Publication number Publication date
NO884429L (en) 1989-04-07
KR970001236B1 (en) 1997-02-04
GB2210620B (en) 1992-07-08
FI884546A0 (en) 1988-10-03
GB8823448D0 (en) 1988-11-16
CN1035845A (en) 1989-09-27
IE60918B1 (en) 1994-08-24
JPH02444A (en) 1990-01-05
SE8803528L (en) 1989-04-07
NZ226486A (en) 1991-08-27
ES2012556A6 (en) 1990-04-01
DK556388D0 (en) 1988-10-05
DE3833897A1 (en) 1989-05-03
MY103779A (en) 1993-09-30
FR2621324A1 (en) 1989-04-07
IT8822213A0 (en) 1988-10-06
BE1002693A5 (en) 1991-05-07
CH680002A5 (en) 1992-05-29
GB2210620B8 (en) 1995-07-17
AT398433B (en) 1994-12-27
DK556388A (en) 1989-04-07
NL8802442A (en) 1989-05-01
IE883018L (en) 1989-04-06
FI884546A (en) 1989-04-07
ATA245488A (en) 1994-04-15
DE3833897C2 (en) 1997-03-20
NO884429D0 (en) 1988-10-05
GR1000492B (en) 1992-07-30
IL87877A0 (en) 1989-03-31
IT1227957B (en) 1991-05-20
KR890006814A (en) 1989-06-16
GB2210620A (en) 1989-06-14
FR2621324B1 (en) 1994-02-18
PT88670B (en) 1992-12-31

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