SE452197B - Elektroforetisk gel samt anvendning av densamma - Google Patents
Elektroforetisk gel samt anvendning av densammaInfo
- Publication number
- SE452197B SE452197B SE8205388A SE8205388A SE452197B SE 452197 B SE452197 B SE 452197B SE 8205388 A SE8205388 A SE 8205388A SE 8205388 A SE8205388 A SE 8205388A SE 452197 B SE452197 B SE 452197B
- Authority
- SE
- Sweden
- Prior art keywords
- electrophoretic gel
- acid
- electrophoretic
- gel according
- agarose
- Prior art date
Links
- 239000000499 gel Substances 0.000 claims description 47
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 21
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims description 14
- 239000002253 acid Substances 0.000 claims description 14
- 229920000936 Agarose Polymers 0.000 claims description 11
- 229920001284 acidic polysaccharide Polymers 0.000 claims description 11
- 150000004805 acidic polysaccharides Chemical class 0.000 claims description 11
- 238000001962 electrophoresis Methods 0.000 claims description 11
- QXKAIJAYHKCRRA-JJYYJPOSSA-N D-arabinonic acid Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C(O)=O QXKAIJAYHKCRRA-JJYYJPOSSA-N 0.000 claims description 10
- 150000003839 salts Chemical class 0.000 claims description 8
- 239000000872 buffer Substances 0.000 claims description 6
- 229920001282 polysaccharide Polymers 0.000 claims description 6
- 239000005017 polysaccharide Substances 0.000 claims description 6
- 150000004804 polysaccharides Chemical class 0.000 claims description 6
- -1 alkyl polyol Chemical class 0.000 claims description 5
- 125000004432 carbon atom Chemical group C* 0.000 claims description 5
- 229920005862 polyol Polymers 0.000 claims description 5
- 240000006240 Linum usitatissimum Species 0.000 claims description 4
- 235000004431 Linum usitatissimum Nutrition 0.000 claims description 4
- 239000003755 preservative agent Substances 0.000 claims description 4
- 102000004169 proteins and genes Human genes 0.000 claims description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 4
- 241000557752 Khaya Species 0.000 claims description 3
- 239000007982 barbital buffer Substances 0.000 claims description 3
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 3
- 235000004426 flaxseed Nutrition 0.000 claims description 3
- 230000002335 preservative effect Effects 0.000 claims description 3
- 229920001817 Agar Polymers 0.000 claims description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical class [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical class C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
- 229920002230 Pectic acid Polymers 0.000 claims description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical class [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 2
- 239000008272 agar Substances 0.000 claims description 2
- 239000000783 alginic acid Substances 0.000 claims description 2
- 235000010443 alginic acid Nutrition 0.000 claims description 2
- 229920000615 alginic acid Polymers 0.000 claims description 2
- 229960001126 alginic acid Drugs 0.000 claims description 2
- 150000004781 alginic acids Chemical class 0.000 claims description 2
- 239000011575 calcium Chemical class 0.000 claims description 2
- 229910052791 calcium Inorganic materials 0.000 claims description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 2
- LCLHHZYHLXDRQG-ZNKJPWOQSA-N pectic acid Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)O[C@H](C(O)=O)[C@@H]1OC1[C@H](O)[C@@H](O)[C@@H](OC2[C@@H]([C@@H](O)[C@@H](O)[C@H](O2)C(O)=O)O)[C@@H](C(O)=O)O1 LCLHHZYHLXDRQG-ZNKJPWOQSA-N 0.000 claims description 2
- 239000010318 polygalacturonic acid Substances 0.000 claims description 2
- 239000011591 potassium Chemical class 0.000 claims description 2
- 229910052700 potassium Inorganic materials 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims description 2
- 229910052708 sodium Inorganic materials 0.000 claims description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical class [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims 1
- 239000011777 magnesium Chemical class 0.000 claims 1
- 229910052749 magnesium Chemical class 0.000 claims 1
- 102000004506 Blood Proteins Human genes 0.000 description 12
- 108010017384 Blood Proteins Proteins 0.000 description 12
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 7
- 238000000034 method Methods 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 229960000583 acetic acid Drugs 0.000 description 3
- 239000000470 constituent Substances 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 235000019441 ethanol Nutrition 0.000 description 2
- 239000012362 glacial acetic acid Substances 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 244000215068 Acacia senegal Species 0.000 description 1
- 229920000084 Gum arabic Polymers 0.000 description 1
- 229920001615 Tragacanth Polymers 0.000 description 1
- 239000000205 acacia gum Substances 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000000305 astragalus gummifer gum Substances 0.000 description 1
- 210000000941 bile Anatomy 0.000 description 1
- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical compound CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000005370 electroosmosis Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 150000002484 inorganic compounds Chemical class 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 159000000003 magnesium salts Chemical class 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 150000002896 organic halogen compounds Chemical class 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- UWJJYHHHVWZFEP-UHFFFAOYSA-N pentane-1,1-diol Chemical compound CCCCC(O)O UWJJYHHHVWZFEP-UHFFFAOYSA-N 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical compound CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Dispersion Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US06/226,566 US4319976A (en) | 1981-01-19 | 1981-01-19 | Electrophoretic technique for separating serum proteins and improved electrophoretic gel for use therein |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| SE8205388L SE8205388L (sv) | 1982-09-20 |
| SE8205388D0 SE8205388D0 (sv) | 1982-09-20 |
| SE452197B true SE452197B (sv) | 1987-11-16 |
Family
ID=22849431
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE8205388A SE452197B (sv) | 1981-01-19 | 1982-09-20 | Elektroforetisk gel samt anvendning av densamma |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US4319976A (de) |
| DE (1) | DE3231627C2 (de) |
| IT (1) | IT1151101B (de) |
| SE (1) | SE452197B (de) |
| WO (1) | WO1982002599A1 (de) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1985004251A1 (en) * | 1984-03-12 | 1985-09-26 | Beckman Instruments, Inc. | Electrophoretic technique for separating hemoglobin variants and improved electrophoretic gel for use therein |
| US4808288A (en) * | 1984-03-12 | 1989-02-28 | Beckman Instruments, Inc. | Electrophoretic gel for separating hemoglobin variants |
| EP0162657B1 (de) * | 1984-05-14 | 1991-02-06 | Fuji Photo Film Co., Ltd. | Elektrophoretischer Bestandteil |
| JPS6266153A (ja) * | 1985-09-18 | 1987-03-25 | Fuji Photo Film Co Ltd | 電気泳動用媒体 |
| US5144013A (en) * | 1986-09-24 | 1992-09-01 | Ube Industries, Ltd. | Body fluid purifying material and method for purifying body fluid by use thereof |
| US4997537A (en) * | 1986-10-21 | 1991-03-05 | Northeastern University | High performance microcapillary gel electrophoresis |
| US5665216A (en) * | 1986-10-21 | 1997-09-09 | Northeastern University | Capillary column for high performance electrophoretic separation and detection of SDS proteins and system and using the same |
| US4857163A (en) * | 1987-07-17 | 1989-08-15 | Beckman Instruments, Inc. | High resolution electrophoretic gel and method for separating serum proteins |
| US4999340A (en) * | 1988-08-04 | 1991-03-12 | Board Of Regents, The University Of Texas System | Rehydratable agarose gels |
| FR2684998B1 (fr) * | 1991-12-11 | 1994-10-28 | Sebia Sa | Procede de separation de la lp(a) par electrophorese, gels pour la mise en óoeuvre de ce procede, et application a la determination in vitro du risque atherogene lie a la presence de la lp(a). |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3527712A (en) * | 1967-03-07 | 1970-09-08 | Marine Colloids Inc | Dried agarose gel,method of preparation thereof,and production of aqueous agarose gel |
| US3959251A (en) * | 1970-06-25 | 1976-05-25 | Exploaterings Aktiebolaget T.B.F. | Stabilized agar product and method for its stabilization |
| US3956273A (en) * | 1971-06-07 | 1976-05-11 | Marine Colloids, Inc. | Modified agarose and agar and method of making same |
| GB1435508A (en) * | 1972-05-01 | 1976-05-12 | Rech Et Dapplications Scient S | Process for the preparation of crosslinked polysaccharide gels |
| US3766047A (en) * | 1972-09-11 | 1973-10-16 | F Elevitch | Gel for electrophoresis |
| US3947352A (en) * | 1974-05-31 | 1976-03-30 | Pedro Cuatrecasas | Polysaccharide matrices for use as adsorbents in affinity chromatography techniques |
| US4305798A (en) * | 1980-04-11 | 1981-12-15 | Bryce Allen Cunningham | Method of preparative, Kohlrausch-regulated electrophoresis using polyethylene glycol derivatized trailing ions |
-
1981
- 1981-01-19 US US06/226,566 patent/US4319976A/en not_active Expired - Lifetime
-
1982
- 1982-01-11 WO PCT/US1982/000022 patent/WO1982002599A1/en active Application Filing
- 1982-01-11 DE DE19823231627 patent/DE3231627C2/de not_active Expired
- 1982-01-19 IT IT19190/82A patent/IT1151101B/it active
- 1982-09-20 SE SE8205388A patent/SE452197B/sv unknown
Also Published As
| Publication number | Publication date |
|---|---|
| SE8205388L (sv) | 1982-09-20 |
| WO1982002599A1 (en) | 1982-08-05 |
| DE3231627C2 (de) | 1987-06-19 |
| IT8219190A0 (it) | 1982-01-19 |
| IT1151101B (it) | 1986-12-17 |
| US4319976A (en) | 1982-03-16 |
| DE3231627T1 (de) | 1983-12-01 |
| SE8205388D0 (sv) | 1982-09-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| NAL | Patent in force |
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