RU2801945C1 - Method of producing a medicinal product for the treatment of acute postpartum endometritis in cows - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: invention relates to a method of producing a medicinal product with a pronounced anti-inflammatory, antiseptic and antifungal effect. A method of producing a medicinal product for the treatment of acute postpartum endometritis in cows, including the preparation of solutions of florfenicol and an antifungal active substance, the use of propylene glycol and purified water as a solvent, characterized in that an additional tetrachloride solution containing 4 g/l of the active substance and a florfenicol solution containing 4 g/l of the active substance, and as an anti-flexible active substance, a 3% aqueous solution of a mixture of sodium benzoate and potassium sorbate is used, which are taken in equal proportions, then all solutions are combined in equal amounts, novocaine, hellebore tincture are added and the resulting mixture is heated to 55–60°C followed by intensive stirring of this mixture in the reactor at a speed of 1,500 rpm, with the following ratio of components, wt.%: florfenicol solution containing 4 g/l of active substance 3, tetrachloride solution containing 4 g/l of active substance 3, 3 % aqueous solution of a mixture of sodium benzoate and potassium sorbate, taken in equal proportions 3, novocaine 1, hellebore tincture 1, purified water 15, propylene glycol is the rest.

EFFECT: increasing the therapeutic effect by enhancing the impact on opportunistic microflora, reducing the duration of treatment and costs.

1 cl, 2 tbl, 3 ex

Description

The invention relates to veterinary medicine, in particular to a method for producing a drug with a pronounced anti-inflammatory, antiseptic and antifungal effect.

A known method of obtaining a drug for the treatment and prevention of postpartum endometritis in cows, including mixing formalin with analgin, hellebore tincture, glycerin, citric acid and gelatin, followed by heating and cooling (RF patent No. 2265433, class A61K 35/50, 2005) .

Also known is a method for producing a drug intended for the treatment of endometritis in cows, including mixing plant components, obtaining an aqueous solution of the drug from a mixture of biological material and succinic acid, obtaining an extract of a medicinal plant to which purified water and an antiseptic are added, then the resulting solution is centrifuged at a speed 500 rpm, the aqueous fraction is collected and dried (RF patent No. 2769508, class A61K 35/50, 2022). However, the known technical solutions do not have an antifungal effect, because with prolonged use of antibiotics, the course of endometritis can be complicated by pathogenic fungi, in particular the genus Candida, Aspergillus, etc.

The closest in technical essence is the invention (RF patent No. 2613141, class A61K 31/395, 2017 - prototype), including the preparation of solutions of florfenicol and antifungal active substance, the use of propylene glycol and purified water as a solvent.

When using a drug obtained by a known method, an insufficient therapeutic effect is manifested, due to a weak effect on the opportunistic microflora that causes infertility in cows after endometritis treatment, a long period of treatment and high costs.

The technical result is to increase the therapeutic effect by increasing the impact on opportunistic microflora, reducing the duration of treatment and costs.

The technical result is achieved by the fact that in a method for producing a drug for the treatment of acute postpartum endometritis in cows, including the preparation of solutions of florfenicol and an antifungal active substance, the use of propylene glycol and purified water as a solvent, according to the invention, an additional tetrachloride solution is used containing 4 g/l of the active substance and florfenicol solution containing 4 g/l of the active ingredient, and as an anti-flexing active ingredient, a 3% aqueous solution of a mixture of sodium benzoate and potassium sorbate is used, which are taken in equal proportions, then all solutions are combined in equal amounts and novocaine, hellebore tincture are added, followed by intensive mixing of the resulting mixture in the reactor at a speed of 1500 rpm, in the following ratio, wt. %:

florfenicol solution containing 4 g/l of active substance 3 tetrachloride solution containing 4 g/l active ingredient 3 3% aqueous solution of a mixture of sodium benzoate and potassium sorbate 3 novocaine 1 hellebore tincture 1 purified water 15 propylene glycol rest.

The novelty of the claimed technical solution lies in the fact that in the claimed method of obtaining a medicinal product, the use of solutions of florfenicol and tetrachloride with sodium benzoate and potassium sorbate and hellebore alkaloids, there is a synergism of the components used to prepare the drug, the properties of which are manifested in the expansion of the antimicrobial spectrum of action, additional fungistatic effect and reflexively increase the motility of the uterus and the secretion of the mucous membrane, which ensures the achievement of the desired technical result.

Floorfenicol has a wide range of action, active in relation to Actinobacillus pleuropneumoniae, Pasteurella spp., Borgetella bronchoseptica, haemophilus spp., Staphylococcus spp., Streptococcus spp., Fusobacterium Necrophorum, Klebciella pneumoniae, Moraxella spp, as well as M. Hyopneumoniae and M-Coplasma hyorhinis, including bacteria that produce acetyltransferase and are resistant to thiamphenicol.

Tetrachloride is a veterinary dosage form of tetracycline hydrochloride. Yellow crystalline powder, soluble in water. 1 g of tetrachloride contains at least 900 mg of tetracycline hydrochloride.

Sodium benzoate is a preservative with the number E211. It is most widely used in acidic foods such as salad dressings (similar to acetic acid), sodas, jams and fruit juices, pickles, frozen yogurt dressings and toppings, as well as meat and fish products, margarine, mayonnaise, and ketchup. .

Potassium sorbate is a potassium salt of sorbic acid obtained by neutralizing sorbic acid with potassium hydroxide. Widely used as a preservative (E202) in food products. It is a white powder or odorless granules with a neutral taste. It is highly soluble in water, withstands heat treatment, is able to mix quickly with the consistency of products and does not affect their taste.

Sodium benzoate and potassium sorbate are approved for use as excipients in dosage forms to stabilize pharmaceuticals.

The method of obtaining a drug for the treatment of acute postpartum endometritis in cows is as follows.

A florfenicol solution containing 4 g/l of the active ingredient is preliminarily prepared on the basis of purified water, a tetrachloride solution containing 4 g/l of the active ingredient and a 3% aqueous solution of a mixture of sodium benzoate and potassium sorbate used as an antifungal active ingredient, which are taken in equal proportions . Then novocaine, hellebore tincture and propylene glycol are added, the resulting mixture is heated to 55-60°C. The selected heating temperature range of the resulting mixture is due to the fact that at temperatures below 55°C, the mixture remains viscous and mixing is difficult, resulting in an inhomogeneous mixture. At temperatures above 60°C, the useful properties of the components included in the mixture are lost. Then it is intensively stirred in the reactor at a speed of 1500 rpm. The result is a viscous, yellowish homogeneous liquid.

To obtain 1000 ml of the drug, three solutions are prepared:

Solution No. 1: Florfenicol powder is dissolved in purified water in a ratio of 1:3 to obtain a solution of florfenicol containing 4 g/l of the active substance.

Solution No. 2: tetrachloride powder is dissolved in purified water in a ratio of 1:3 to obtain a tetrachloride solution containing 4 g/l of the active substance.

Solution No. 3: powders of sodium benzoate and potassium sorbate are separately dissolved in purified water in a ratio of 1:3, then they are taken in equal proportions and mixed.

Next, the resulting solutions are combined in equal amounts and novocaine, hellebore tincture, 100 ml/l, and propylene glycol, up to 1 liter, are added, followed by vigorous stirring.

The resulting agent is used intrauterinely in compliance with the rules of asepsis and antisepsis.

The antimicrobial and antifungal activity of the drug was tested with different content of its components and compared with the prototype.

Examples of obtaining the claimed invention.

Example 1. Obtaining a medicinal product containing, wt. %: florfenicol 1.0, tetrachloride 1.0, sodium benzoate 1.0, potassium sorbate 1.0, hellebore tincture 1, novocaine 1.0 and propylene glycol - the rest.

Example 2. Obtaining a drug, wt. %: wt. %: containing florfenicol 3.0, tetrachloride 3.0, sodium benzoate 3.0, potassium sorbate 3.0, hellebore tincture 1 ml, novocaine 1.0 and propylene glycol - the rest.

Example 3. Obtaining a medicinal product wt. %: containing florfenicol 5.0, tetrachloride 5.0, sodium benzoate 5.0, potassium sorbate 5.0, hellebore tincture 1.0, novocaine 1.0 g and propylene glycol - the rest.

Data from the study of microbial activity are shown in table 1

Since the effectiveness of the claimed agent is almost the same with different contents of its components, example 2 was chosen as the optimal one. Examples of the use of the claimed medicinal preparation 60 animals were involved in the experiment. The experiment was carried out in two series.

In the first series of experiments, animals with acute postpartum endometritis (30 cows) were used.

In the second series of experiments - the prevention of endometritis (30 cows).

Both in the first and in the second series of experiments, the animals were divided into two equal groups - experimental and control. Animals of the experimental group were injected with the claimed medicinal product, and the animals of the control group - the drug of the prototype according to the instructions for use of the drug. Animals were constantly monitored.

Example 1. The use of a drug for the treatment of acute postpartum endometritis (complicated by microflora).

The drugs were administered at a dose of 50 ml intrauterine with an interval of 72 hours in combination with vitamin therapy. In the experiment, 2 injections of the drug were required to cure the animals, and 4 in the control.

Example 2

The drug was administered to 15 cows to prevent postpartum endometritis during the first 12 hours after calving at a dose of 100 ml once intrauterine. The control used the preparation of the prototype (a similar number of animals).

In the experimental group, the indicators of the preventive efficacy of the drug exceeded the data of the prototype by 14%.

Example 3

The drug was administered to 15 cows for the treatment of postpartum acute endometritis (complicated by microflora in association with fungi of the genus Candida, etc.). The drugs were administered at a dose of 50 ml intrauterine with an interval of 72 hours in combination with vitamin therapy. In the experiment, 2-3 injections of the drug were required to cure the animals, and 5-6 in the control.

Clinical observations and studies have shown that after application to cows with postpartum acute endometritis in a comparative experiment of the test drug in all experimental animals, the manifestation of the stage of excitation of the sexual cycle was recorded.

The results of fertility in the treatment of cows with postpartum acute endometritis with the drug obtained by the claimed method are presented in table 2.

Approbation of the drug for the treatment of acute postpartum endometritis in cows showed that the number of drug injections and the number of days of treatment decreased. Thus, the use of this drug is the most cost-effective and creates an optimal therapeutic effect.

Thus, a higher (100%) fertility was found in cows that were treated with a drug obtained by the claimed method from postpartum acute endometritis, they have an insemination index of 1.8.

It should be noted that in the experimental group, the highest fertility was in the first and second estrus, which are decisive for artificial insemination of cows (32.0-48.0%, respectively).

Claims (2)

A method for producing a drug for the treatment of acute postpartum endometritis in cows, including the preparation of solutions of florfenicol and an antifungal active substance, the use of propylene glycol and purified water as a solvent, characterized in that an additional solution of tetrachloride containing 4 g/l of the active substance and a solution of florfenicol containing 4 g / l of the active substance, and as an anti-flexible active substance, a 3% aqueous solution of a mixture of sodium benzoate and potassium sorbate is used, which are taken in equal proportions, then all solutions are combined in equal amounts, novocaine, hellebore tincture are added and the resulting mixture is heated to 55 - 60 °C followed by intensive mixing of this mixture in the reactor at a speed of 1500 rpm, with the following ratio of components, wt.%: florfenicol solution containing 4 g/l active substance 3 tetrachloride solution containing 4 g/l active substance 3 3% aqueous solution of sodium benzoate mixture and potassium sorbate, taken in equal proportions 3 novocaine 1 hellebore tincture 1 purified water 15 propylene glycol rest