RU2755345C2 - Эффективная вакцинация против европейских штаммов вируса репродуктивного и респираторного синдрома (prrs) до отлучения от матери - Google Patents
Эффективная вакцинация против европейских штаммов вируса репродуктивного и респираторного синдрома (prrs) до отлучения от матери Download PDFInfo
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- A61K2039/55—Medicinal preparations containing antigens or antibodies characterised by the host/recipient, e.g. newborn with maternal antibodies
- A61K2039/552—Veterinary vaccine
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C12N2770/10034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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Landscapes
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- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
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Applications Claiming Priority (3)
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| US201662434144P | 2016-12-14 | 2016-12-14 | |
| US62/434,144 | 2016-12-14 | ||
| PCT/US2017/066347 WO2018112169A2 (en) | 2016-12-14 | 2017-12-14 | Effective vaccination against european strains of porcine reproductive and respiratory syndrome (prrs) virus prior to weaning |
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| RU2019117647A RU2019117647A (ru) | 2021-01-15 |
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| EP (1) | EP3554537A2 (https=) |
| JP (1) | JP7129978B2 (https=) |
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| KR102012109B1 (ko) * | 2012-05-17 | 2019-08-19 | 조에티스 엘엘씨 | 이유 전 돼지 생식기 호흡기 증후군(prrs) 바이러스에 대한 효과적인 백신접종 |
| MX2019006992A (es) * | 2016-12-14 | 2019-08-29 | Zoetis Services Llc | Vacunacion eficaz contra cepas europeas del virus del sindrome reproductivo y respiratorio porcino (prrs) previo al destete. |
| CN119876049A (zh) | 2019-08-29 | 2025-04-25 | 礼蓝美国公司 | 猪繁殖与呼吸综合征疫苗病毒 |
| JP7369343B2 (ja) * | 2019-10-11 | 2023-10-26 | 国立研究開発法人農業・食品産業技術総合研究機構 | 豚感染ウイルスの製造方法 |
| CN111826468A (zh) * | 2020-08-07 | 2020-10-27 | 中国农业科学院兰州兽医研究所 | 一步法检测1型猪繁殖与呼吸综合征病毒的引物探针组和试剂盒 |
| EP4630048A1 (en) * | 2022-12-06 | 2025-10-15 | Zoetis Services LLC | Methods and compositions for vaccinating piglets against prrs-1 virus |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013173443A1 (en) * | 2012-05-17 | 2013-11-21 | Zoetis Llc | Effective vaccination against porcine reproductive and respiratory syndrome (prrs) virus prior to weaning |
| RU2561595C2 (ru) * | 2008-08-25 | 2015-08-27 | Бёрингер Ингельхайм Ветмедика, Инк. | Вакцина против высокопатогенного репродуктивно-респираторного синдрома свиней (hp prrs) |
| WO2016012406A2 (en) * | 2014-07-21 | 2016-01-28 | Istituto Zooprofilattico Sperimentale Della Lombardia E Dell'emilia Romagna "Bruno Ubertini" (Izsler) | Attenuated strain of prrs and potential use in immunising preparations |
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| US3137631A (en) | 1959-12-01 | 1964-06-16 | Faberge Inc | Encapsulation in natural products |
| US3519710A (en) | 1967-08-10 | 1970-07-07 | Edmund P Bass | Direct active modified live virus vaccine immunization against transmissible gastroenteritis in swine piglets at birth |
| US3959457A (en) | 1970-06-05 | 1976-05-25 | Temple University | Microparticulate material and method of making such material |
| JPS5186117A (en) | 1975-01-27 | 1976-07-28 | Tanabe Seiyaku Co | Johoseibiryushiseizainoseiho |
| US4205060A (en) | 1978-12-20 | 1980-05-27 | Pennwalt Corporation | Microcapsules containing medicament-polymer salt having a water-insoluble polymer sheath, their production and their use |
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| US4744933A (en) | 1984-02-15 | 1988-05-17 | Massachusetts Institute Of Technology | Process for encapsulation and encapsulated active material system |
| US5008050A (en) | 1984-06-20 | 1991-04-16 | The Liposome Company, Inc. | Extrusion technique for producing unilamellar vesicles |
| US4921706A (en) | 1984-11-20 | 1990-05-01 | Massachusetts Institute Of Technology | Unilamellar lipid vesicles and method for their formation |
| US4606940A (en) | 1984-12-21 | 1986-08-19 | The Ohio State University Research Foundation | Small particle formation and encapsulation |
| US5009956A (en) | 1987-02-24 | 1991-04-23 | Univ Minnesota | Phospholipase A2-resistant liposomes |
| US4927637A (en) | 1989-01-17 | 1990-05-22 | Liposome Technology, Inc. | Liposome extrusion method |
| US4944948A (en) | 1989-02-24 | 1990-07-31 | Liposome Technology, Inc. | EGF/Liposome gel composition and method |
| US5132117A (en) | 1990-01-11 | 1992-07-21 | Temple University | Aqueous core microcapsules and method for their preparation |
| JP3128133B2 (ja) | 1991-06-06 | 2001-01-29 | スティッチティング セントラール ディールゲネースクンディグ インスティトゥート | 豚の奇病の原因体であるレリスタドエイジェント、それを含むワクチン組成物、豚の奇病の診断キット及び診断方法 |
| KR0138092B1 (ko) | 1991-08-26 | 1998-04-30 | 제임스 씨. 데세사레 | 미확인(Mystery) 돼지 질병 관련 바이러스제 |
| DK0601062T4 (da) | 1991-08-26 | 2001-03-14 | Univ Minnesota | SIRS-vaccine og diagnosefremgangsmåde |
| US5846805A (en) | 1991-08-26 | 1998-12-08 | Boehringer Ingelheim Animal Health, Inc. | Culture of swine infertility and respiratory syndrome virus in simian cells |
| US6042830A (en) | 1992-08-05 | 2000-03-28 | Boehringer Ingelheim Vetmedica, Inc. | Viral agent associated with mystery swine disease |
| US6592873B1 (en) | 1992-10-30 | 2003-07-15 | Iowa State University Research Foundation, Inc. | Polynucleic acids isolated from a porcine reproductive and respiratory syndrome virus (PRRSV) and proteins encoded by the polynucleic acids |
| US6251397B1 (en) | 1992-10-30 | 2001-06-26 | Iowa State University Research Foundation, Inc. | Proteins encoded by polynucleic acids isolated from a porcine reproductive and respiratory syndrome virus and immunogenic compositions containing the same |
| US20040087521A1 (en) | 1993-03-18 | 2004-05-06 | Merck & Co., Inc. | Nucleic acid pharmaceuticals-influenza matrix |
| US6015686A (en) | 1993-09-15 | 2000-01-18 | Chiron Viagene, Inc. | Eukaryotic layered vector initiation systems |
| US5789543A (en) | 1993-12-30 | 1998-08-04 | President And Fellows Of Harvard College | Vertebrate embryonic pattern-inducing proteins and uses related thereto |
| JPH10500889A (ja) | 1994-04-15 | 1998-01-27 | テンプル・ユニバーシティ | 水性溶媒封入法、装置およびマイクロカプセル |
| PT702085E (pt) | 1994-07-18 | 2004-04-30 | Karl Klaus Conzelmann | Virus de arn de cadeia negativa nao segmentada infeccioso recombinante |
| WO1996004010A1 (en) | 1994-08-05 | 1996-02-15 | Regents Of The University Of Minnesota | Vr-2332 viral nucleotide sequence and methods of use |
| GB9518220D0 (en) | 1995-09-06 | 1995-11-08 | Medical Res Council | Checkpoint gene |
| US5866401A (en) | 1996-03-01 | 1999-02-02 | Schering Corporation | Porcine reproductive and respiratory syndrome vaccine |
| JPH09313188A (ja) | 1996-05-31 | 1997-12-09 | Kazuaki Chayama | Ｇ型肝炎ウイルス遺伝子 |
| EP0839912A1 (en) | 1996-10-30 | 1998-05-06 | Instituut Voor Dierhouderij En Diergezondheid (Id-Dlo) | Infectious clones of RNA viruses and vaccines and diagnostic assays derived thereof |
| WO1998055626A2 (en) | 1997-06-05 | 1998-12-10 | Origen, Inc. | Recombinant porcine reproductive and respiratory syndrome virus (prrsv) for use as a vaccine |
| AUPO856097A0 (en) | 1997-08-14 | 1997-09-04 | Commonwealth Scientific And Industrial Research Organisation | Vector |
| US5951977A (en) | 1997-10-14 | 1999-09-14 | The United States Of America, As Represented By The Secretary Of Agriculture | Competitive exclusion culture for swine |
| US7691389B2 (en) | 1998-12-22 | 2010-04-06 | Pfizer Inc | Infectious cDNA clone of north american porcine reproductive and respiratory syndrome (PRRS) virus and uses thereof |
| US7618797B2 (en) | 1998-12-22 | 2009-11-17 | Pfizer Inc | Infectious cDNA clone of North American porcine reproductive and respiratory syndrome (PRRS) virus and uses thereof |
| US7132106B2 (en) | 1998-12-22 | 2006-11-07 | Pfizer Inc. | Infectious cDNA clone of North American porcine reproductive and respiratory syndrome (PRRS) virus and uses thereof |
| CA2290220C (en) | 1998-12-22 | 2013-11-19 | Pfizer Products Inc. | An infectious cdna clone of north american porcine reproductive and respiratory syndrome (prrs) virus and uses thereof |
| US6943152B1 (en) | 1999-06-10 | 2005-09-13 | Merial | DNA vaccine-PCV |
| WO2005107796A2 (en) | 2004-04-23 | 2005-11-17 | Pharmacia & Upjohn Company, Llc | Cellular permissivity factor for viruses, and uses thereof |
| PL2369001T3 (pl) | 2005-06-24 | 2017-06-30 | Regents Of The University Of Minnesota | Wirusy PRRS, ich klony zakaźne, mutanty i sposoby stosowania |
| PL2106440T3 (pl) | 2007-01-12 | 2016-02-29 | Univ Illinois | Komórki niepochodzące od małp do hodowli wirusa zespołu rozrodczo-oddechowego świń (PRRS) |
| CA2694476C (en) | 2007-07-27 | 2017-06-27 | Universiteit Gent | Permissive cells and uses thereof |
| UA108902C2 (uk) * | 2010-11-10 | 2015-06-25 | Вірус північноамериканського репродуктивного та респіраторного синдрому свиней (prrs) та його застосування | |
| HRP20151031T1 (hr) * | 2011-02-17 | 2015-11-06 | Boehringer Ingelheim Vetmedica Gmbh | Novi europski soj prrsv |
| UA114504C2 (uk) * | 2012-04-04 | 2017-06-26 | Зоетіс Сервісіз Ллс | Комбінована вакцина pcv, mycoplasma hyopneumoniae та prrs |
| MX2019006992A (es) * | 2016-12-14 | 2019-08-29 | Zoetis Services Llc | Vacunacion eficaz contra cepas europeas del virus del sindrome reproductivo y respiratorio porcino (prrs) previo al destete. |
-
2017
- 2017-12-14 MX MX2019006992A patent/MX2019006992A/es unknown
- 2017-12-14 AU AU2017378336A patent/AU2017378336B2/en active Active
- 2017-12-14 EP EP17829078.9A patent/EP3554537A2/en active Pending
- 2017-12-14 BR BR112019012158-7A patent/BR112019012158A2/pt unknown
- 2017-12-14 RU RU2019117647A patent/RU2755345C2/ru active
- 2017-12-14 UA UAA201906661A patent/UA124976C2/uk unknown
- 2017-12-14 KR KR1020197017275A patent/KR20190085977A/ko not_active Ceased
- 2017-12-14 CA CA3047294A patent/CA3047294A1/en active Pending
- 2017-12-14 CN CN201780077455.5A patent/CN110072547B/zh active Active
- 2017-12-14 JP JP2019532094A patent/JP7129978B2/ja active Active
- 2017-12-14 US US16/469,321 patent/US11090376B2/en active Active
- 2017-12-14 KR KR1020217030759A patent/KR102602018B1/ko active Active
- 2017-12-14 WO PCT/US2017/066347 patent/WO2018112169A2/en not_active Ceased
-
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- 2019-06-11 PH PH12019501320A patent/PH12019501320A1/en unknown
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2561595C2 (ru) * | 2008-08-25 | 2015-08-27 | Бёрингер Ингельхайм Ветмедика, Инк. | Вакцина против высокопатогенного репродуктивно-респираторного синдрома свиней (hp prrs) |
| WO2013173443A1 (en) * | 2012-05-17 | 2013-11-21 | Zoetis Llc | Effective vaccination against porcine reproductive and respiratory syndrome (prrs) virus prior to weaning |
| WO2016012406A2 (en) * | 2014-07-21 | 2016-01-28 | Istituto Zooprofilattico Sperimentale Della Lombardia E Dell'emilia Romagna "Bruno Ubertini" (Izsler) | Attenuated strain of prrs and potential use in immunising preparations |
Non-Patent Citations (1)
| Title |
|---|
| BRET O,brien et al., Fostera PRRS vaccine for one-day-old piglets: respiratory efficacy, duration of immunity, and safety research, 01.12.2014, p/ 1-8, DOI:10.1186/1746-6148-10-124, in particular p.7, figures 1-7, tables 1-2. * |
Also Published As
| Publication number | Publication date |
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| EP3554537A2 (en) | 2019-10-23 |
| CN110072547A (zh) | 2019-07-30 |
| KR20190085977A (ko) | 2019-07-19 |
| CN110072547B (zh) | 2024-04-30 |
| US20190365881A1 (en) | 2019-12-05 |
| AU2017378336A1 (en) | 2019-05-30 |
| RU2019117647A (ru) | 2021-01-15 |
| BR112019012158A2 (pt) | 2019-11-12 |
| KR102602018B1 (ko) | 2023-11-15 |
| CA3047294A1 (en) | 2018-06-21 |
| JP2020502158A (ja) | 2020-01-23 |
| AU2017378336B2 (en) | 2024-06-06 |
| MX2019006992A (es) | 2019-08-29 |
| JP7129978B2 (ja) | 2022-09-02 |
| KR20210121293A (ko) | 2021-10-07 |
| UA124976C2 (uk) | 2021-12-22 |
| WO2018112169A2 (en) | 2018-06-21 |
| WO2018112169A3 (en) | 2018-07-26 |
| RU2019117647A3 (https=) | 2021-01-20 |
| PH12019501320A1 (en) | 2020-03-02 |
| US11090376B2 (en) | 2021-08-17 |
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