RU2748469C1 - Insectoacaricide paste - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: invention relates to veterinary medicine, in particular to drugs that have an insectoacaricide effect. Insectoacaricide paste contains by wt.%: thymol-B - 3.85, lanolin - 67.3, natural beeswax - 28.85.

EFFECT: invention makes it possible to obtain an insectoacaricide drug that is safe for animals and has increased efficiency.

1 cl, 2 dwg, 7 ex

Description

The technical field to which the invention relates

The invention relates to veterinary medicine, in particular to drugs that have an insectoacaricidal effect, which consists of the main action in preventing the attack of insects and ticks, carriers of dangerous infectious and invasive diseases.

State of the art

Currently, many scientists are working on the development and improvement of new ways to combat animal ectoparasites.

Known emulsion composition (patent RU 2575571, IPC A61K 31/235, A61K 9/10, A61P 17/04, publ. 02/20/2016, bull. No. 5) for external use with acaricidal anti-scab pharmacological activity, characterized by the fact that it contains benzyl benzoate, sodium stearate, emulsion wax, bronopol and water in the following ratio of components, wt%:

Benzyl benzoate - 10.0-22.0

Sodium stearate - 0.1-0.25

Emulsion wax - 0.9-1.1

Bronopol - 0.09-0.11

Water - up to 100.0.

The disadvantage of this emulsion composition is that Bronopol is a chemical preservative, which, upon prolonged contact with the skin of an animal, can have an irritating effect.

For the prototype was taken a drug for treating dogs and cats against ectoparasites (patent RU 22298923, IPC A01N 25/00, A61K 31/00, publ. 05/20/2007, bull. No. 14) including castor oil, insectoacaricide "Proteid" and natural beeswax , despite the fact that it is additionally supplied with lanolin, and the trimmings of bees' combs are taken as beeswax with the following ratio of components, wt%:

"Proteid" 2-4 bee honeycomb trimmings 55-65 lanolin 26-30 Castor oil rest

The disadvantage of this drug is the use of the insectoacaricide "Proteid", which is toxic to animals, while the cuttings of the honeycomb, in addition to wax, contain the flesh, which is a trash impurity.

Disclosure of invention

The objective of the present invention is to improve the method of manufacturing, application, as well as prolong the effectiveness of a preparation with insectoacaricidal properties for treating animals against insects and ticks.

The technical result of the invention is to determine the optimal formula of the insectoacaricidal preparation, to ensure environmental safety for animals, to reduce the cost, as well as to increase efficiency.

The technical result is achieved with the help of an insectocaricidal paste containing natural beeswax and lanolin, while additionally containing Timol-B, in a weight% ratio:

Timol-B 3.75-3.85 Lanolin 67.2-67.3 Natural beeswax 28.75-28.85

Brief description of drawings and their materials

FIG. 1 - Contact and residual action of insectoacaricidal paste applied to fabric tested on isolated mites;

FIG. 2 - Results of testing insecticidal acaricidal paste on dogs.

Implementation of the invention

Examples of specific manufacture of insecticidal acaricidal paste

Experience 1.

Natural beeswax is introduced into a heat-resistant flask and placed in a water bath at a temperature of 60-65 ° C. Then, after the wax has melted, lanolin is added, with constant stirring with a glass rod until it melts and the two components are mixed until a homogeneous consistency. Then the flask is removed from the water bath, and with constant stirring, thymol-B powder is gradually introduced until it dissolves and poured into a glass container made of dark glass, while the ratio of the components in wt%:

Timol-B 4.0 Lanolin 48.0 Natural beeswax 48.0

After cooling, the insecticidal acaricidal paste is stored at a temperature of + 4 ° C until it is used. The proposed insecticide-acaricidal paste is applied not to the body of animals, but to a “collar” made of cotton fabric with a fastener.

Experience 2.

The preparation of insecticidal acaricidal paste is carried out according to experiment 1, which manifests itself in the following ratio, wt%:

Timol-B 3.75 Lanolin 67.2 Natural beeswax 28.75

Experience 3.

The preparation of insecticidal acaricidal paste is carried out according to experiment 1, which manifests itself in the following ratio, wt%:

Timol-B 3.85 Lanolin 67.3 Natural beeswax 28.85

Experience 4.

The preparation of insecticidal acaricidal paste is carried out according to experiment 1, which manifests itself in the following ratio, wt%:

Timol-B 3.6 Lanolin 67.5 Natural beeswax 28.9

As a result of the experiments carried out on the selection of the recipe for preparation, it was revealed that experiments No. 2, No. 3 are the most optimal options, which include:

Timol-B 3.75-3.85 Lanolin 67.2-67.3 Natural beeswax 28.75-28.85

Experience No. 1 showed the worst result, due to the weak insect-acaricidal effect on insects and ticks. Experience No. 4 showed that a high concentration of thymol-B in the composition of the insecticidal acaricidal paste causes irritation of the skin in animals.

Experience 5.

In order to find new effective insectoacaricides against insects and ticks, a test is carried out in laboratory conditions: hungry adults were in contact for a day with paper filters soaked in the drug in different concentrations, which were in a Petri dish. Then, new ticks were planted in the same cups on days 3, 5, 7, 9, 11 after the first treatment. The results of the experiments were taken into account once a day by examining the ticks and determining their condition. In the control, distilled water was used instead of the insectoacaricidal paste. The experiment used the drug Timol-B in 3.6, 3.75, 3.85, 4.0% concentrations. The results are shown in FIG. one.

Experience 6.

To test the developed insectoacaricidal paste, 5 groups of dogs, 5 heads each, were formed, one of which was a control. In the first four groups of animals, insecticidal acaricidal paste was used in different concentrations (3.6, 3.75, 3.85, 4.0%) for 14 days. Each dog from 4 groups was put on a cotton "collar" with a fastener impregnated with the developed paste during the walk. In the control group, the collars were not treated. The control was carried out once a day after walking the animal for 1 hour in the forest park area by external examination and combing the wool. These studies are presented in FIG. 2.

As seen from the data in FIG. 2, a paste with a Timol-B content at a concentration of 3.0% has acaricidal properties for 9 days. From the 10th day of observation, when combing the animals, 1-2 individuals of ixodid ticks are found.

A paste with an active substance concentration of 3.5% exhibits properties on ixodid for 14 days, then on dogs, when examined, there are 1-2 adults.

The developed paste with a Timol-B concentration in the amount of 4% showed acaricidal properties against imago ixodid ticks during the entire study period.

Thus, the cape we developed, impregnated with an acaricidal paste consisting of lanolin, natural beeswax and Timol-B at a concentration of 4%, has the greatest effect against imago ixodid ticks, which provided protection to the dogs throughout the entire study period for 14 days.

Experience 7.

Determination of toxicity in laboratory white mice and rats. Toxicity was determined by skin test in laboratory mice and rats. For this, 2 groups of each animal were formed: the first - experimental, the second - control. The developed insectoacaricidal paste was applied to mice and rats from the first group twice on the shaved areas of the animal's body measuring 1 × 1 cm in the abdomen. Distilled water was applied to the animals of the second group. The result of the study was read after 30 minutes, as well as during the day with a frequency of 1 hour. It was found that in the experimental group, inflammatory reactions in the form of hyperemia, tissue edema, and developing necrosis were not revealed. No changes were found in the control group. To prevent laboratory animals from licking the insectoacaricidal paste, they put on a special collar.

Insectoacaricidal paste, in comparison with the prototype, has the following advantages:

- improved manufacturing method;

- the method of application has been improved;

- increased terms of insectoacaricidal action;

- due to the low cost of the initial components of the insectoacaricidal paste, the cost of the final product decreases;

- during the experiment on laboratory animals, it was found that the developed insectoacaricidal paste is environmentally safe.

Claims (2)

Insector caricidal paste containing natural beeswax and lanolin, characterized in that it additionally contains Timol-B, in a wt% ratio: Timol-B 3.85 Lanolin 67.3 Natural beeswax 28.85

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