RU2739295C1 - Antiseptic - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention refers to medicine and can be used for hygienic treatment of hands of medical personnel, in emergency situations zones, as an antimicrobial and bactericidal agent. Antiseptic agent contains water, potassium alum, lanolin, cocoa butter, β-phenyl-ethanol, 20 % aqueous extract of propolis, with the following ratio of ingredients, wt. %: distilled or demineralized water – 90.00–92.00, potassium alum – 1.76–2.00, cocoa butter – 3.12–3.30, β-phenyl-ethanol – 0.20–0.30, 20 % aqueous extract of propolis – 1.01–1.70, lanolin – 1.91–2.70.

EFFECT: agent has effective bactericidal and antiseptic properties, stability of quality indices, which enables to use the agent as a skin antiseptic and a disinfectant for small surfaces.

1 cl, 1 tbl

Description

The invention relates to medicine. It can be used as an antiseptic agent for hygienic treatment of the hands of medical personnel, in emergency zones, and also as an antimicrobial and bactericidal agent to destroy or delay the reproduction of pathogenic microorganisms.

In the State Register of Medicines under the registration certificate number LSR-005774/10, a lapis pencil is used as an antiseptic agent, designed to combat fungi of the genus Trichophyton rubrum, which is a white or grayish-white solid conical stick with a rounded top [Lapis pencil, file : /// C: /Users/User/Desktop/FOR%20PATENT/Lapisny %20pencil%20_%20ЛСР-005774_10%20_%20State%20registry%20drugs%20.html.], [Pencil: instructions for use ,

https://dermgid-com.turbopages.org/dermgid.com/s/lechenie/prochee-lechenie/karandash-lyapisnii.html].

A linen pencil contains 0.18 g of silver nitrate and 0.37 g of potassium nitrate. The disadvantage of the described pencil is a strong burning sensation, allergic skin rashes in places remote from the treatment. Itching, redness, dryness of the skin may occur, as well as the use of expensive silver nitrate as the main substance.

Known antiseptic preparation (Patent RU 2207155, A16L2 / 16, A16L2 / 18, publ. 06/27/2003), containing a mixture of isopropyl, n-propyl alcohols and didecyldimethylammonium chloride, prepared at 25-30 ° C and taken in a ratio of 6-5: 1 -2: 0.005-0.015. The preparation also contains propylene glycol, glycerin and water in certain proportions. The drug has antimicrobial activity against gram-positive and gram-negative bacteria (including causative agents of nosocomial infections; mycobacterium tuberculosis), yeast-like fungi of the genus Candida and viruses (including hepatitis B virus and HIV). The disadvantage of this drug is the side effects of isopropyl and n-propyl alcohols, manifesting in allergic reactions, possible hyperemia and soreness of the skin. When applied externally, it is partially absorbed through the skin and mucous membranes and can have a systemic toxic effect (CNS depression).

The closest is (patent RU 2501556, A61K31 / 197, A61K33 / 06, A61K33, F61P7 / 04, publ. 20.12.2013) an antiseptic preparation containing sodium chloride 0.58-0.71; ε-aminocaproic acid 2.14-3.25; purified water 60.93-73.98; potassium alum 19.65-32.84; lanolin 1.77-2.92.

This drug does not have a wide range of uses. The drug is a hemostatic match with a hard head and with small cuts has anti-inflammatory, hemostatic, disinfectant, absorbing properties. Potassium alum is able to inhibit the secretion of sweat glands without clogging them (unlike formalin-containing products that can be harmful to health). The disadvantage is that they are only able to kill microorganisms, the vital activity of which is the cause of the unpleasant odor.

The objective of the invention is to create a broad spectrum antiseptic preparation with bactericidal and antiseptic properties.

The essence of the invention lies in the fact that an antiseptic agent comprising a solution containing water, potassium alum, lanolin, additionally includes cocoa butter, β-phenyl ethanol, 20% propolis extract, with the following ratio of ingredients, wt%:

distilled or demineralized water 90.00-92.00; potassium alum 1.76-2.00; cacao butter 3.12-3.30; β-phenyl ethanol 0.20-0.30; 20% propolis extract 1.01-1.70; lanolin 1.91-2.70

The technical result is an increase in the antibacterial effect and slowing down the growth of bacteria due to the destructive effect of potassium alum on pathogenic bacteria, the effectiveness of β-phenyl ethanol in relation to gram-positive and gram-negative bacteria, pronounced antibacterial effect of propolis. Cocoa butter and lanolin have an emollient effect on the skin, and lanolin also has an antistatic function.

In total, its action is characterized by effective bactericidal and antiseptic properties.

Component characteristics

Cocoa butter has a solid texture with a yellowish, less often creamy or light brown color. Cocoa butter is based on fatty acids - stearic, oleic, palmitic and linoleic. Of these, oleic is found in the largest amount (almost half of the mass) and is useful in that it can lower cholesterol levels. In addition to fatty acids, cocoa butter contains a large amount of tannins, caffeine and xanthine. These beneficial substances have a very beneficial effect on the condition of the skin when the oil is used in cosmetology for the face, hair and body. Has antiseptic and anti-inflammatory properties [ Healing properties of cocoa butter - treatment of diseases. https://vseprocofe.ru/kakao/lechebnye-svojstva-kakao-masla-lechenie-zabolevanij/] For external use: restores the integrity of the mucous membranes; promotes the regeneration of skin cells; stimulates collagen production; improves hair condition. Cocoa butter has a moisturizing, softening effect on the skin, making it soft, supple and smooth.

β-Phenyl-ethanol C ₆ H ₅ CH ₂ CH ₂ OH is a simple aromatic primary alcohol, an organic compound, contained in pink (25-65%), clove, geranium, nerolium, ylang and other essential oils, in the form of cinnamon ether acids found in resins and balms. Prevents or slows down the growth of bacteria: effective against gram-positive and gram-negative bacteria. [http://eco-naturel.com/k2_334.php].

β-Phenyl-ethanol exhibits moderate antimicrobial activity, maximum at a pH of less than 5.0, at a pH of more than 8.0, it decreases [http://eco-naturel.com/k2_334.php] .

Propolis is a soft substance that thickens over time, becoming a brittle mass from yellow to dark brown. Has a specific characteristic odor.

The organic composition of propolis allows it to ensure safety and harmlessness in the process of practical use. A large proportion of substances are resins; wax accounts for 1/3 of the composition. In addition, propolis contains a large amount of acids:

- pheno acids allow it to have anti-inflammatory, diuretic, choleretic, astringent qualities;

- benzoic acid prevents the development of pathogenic microorganisms in the body and on the skin surface;

- ferulic acid provides comprehensive prevention of bacterial growth and development;

- fatty acids - are proof of antioxidant properties.

Thanks to caffeic acid, it is a good anti-inflammatory agent. Westitol and neovestitol propolis has a pronounced antibacterial effect, increase the rate of migration of neutrophils, have an anti-inflammatory effect [Karomatov ID Propolis: use in medicine / ID Karomatov. - Text: direct // Young scientist. - 2014. - No. 3 (62). - S. 183-199. - URL: https://moluch.ru/archive/62/9593/ (date of access: 09/14/2020] .

A 20% propolis extract is used, for the preparation of which propolis was previously placed in a freezer for 1 hour, after which it was ground with a mortar to a powder fraction. The resulting powder was poured with water and placed in a dark place for 1 hour and, after shaking, the floated waste rock was separated, decanted. High-quality purified propolis settled to the bottom was dried.

To prepare a 20% extract for 80 g of demineralized water, 20 g of propolis was taken and kept in a water bath for at least 3 hours, after which the finished preparation was filtered through a three-layer gauze.

Lanolin is an animal wax obtained from the digestion of sheep wool. A thick, viscous yellow or brownish-yellow mass, with a peculiar smell, melting at a temperature of 36 to 42 ° C. Lanolin is similar in properties to human sebum. It is widely used in the composition of various cosmetics - creams, etc., in medicine it is used as a basis for various ointments (in particular eye), plasters and adhesive bandages, as well as for softening the skin (mixed with an equal amount of petroleum jelly). Produced pure, refined lanolin for breastfeeding women. Applied topically, lanolin helps to heal and prevent nipple fissures, and does not need to be washed off before feeding (not harmful to babies).

The most important functions of lanolin:

- emulsifying (lanolin emulsifies up to 200% of water and up to 140% of glycerin and forms a "water-oil" emulsion);

- softening;

- conditioning (lanolin is an important component in hair conditioners that can improve the structure of hair, cleanse it of dirt and dust);

antistatic [https://lindera.ru/emulgatory-zagustiteli-emolenty/lanolin-phrancija.html].

Potassium alumrepresent minerals of natural origin used in medicine and cosmetology due to their enveloping, anti-inflammatory, cauterizing properties. In appearance, potassium alum is a large, colorless transparent crystals, the taste of which can be characterized as sweet. The mineral has excellent astringent properties, helping to absorb excess moisture without blocking sweat glands. In the process of processing, the crystals are crushed: a white powder (powder) is obtained, the basis for pharmaceuticals.

The antiseptic was prepared as follows:

In a round-bottom three-necked flask was placed 92 g of demineralized water and 1.76 g of potassium alum. The stirrer was turned on and after dissolution of potassium alum at a temperature of 32 ° C, 3.12 g of cocoa butter was added. 0.2 g of β-phenyl-ethanol and 1.01 g of a 20% aqueous propolis extract were added to the resulting emulsion with a stirrer turned on. After 10 minutes of stirring, 1.91 g of lanolin was added to the flask and stirring was continued for another 15 minutes. In this case, the suspension became transparent. The flask was kept at a constant temperature of 32 ° C all the time. The clear solution was poured out of the flask and the stability of the antiseptic disinfectant was assessed by determining the quality indicators (appearance, absence of sediment and turbidity, color, pH, mass fractions of active ingredients: distilled or demineralized water; potassium alum; cocoa butter; β-phenyl ethanol; propolis; lanolin) in accordance with GOST R 58151.1 and clause 4 "Chemical and analytical methods of research of disinfectants" of Manual R 4.2.2643 "Methods of laboratory research and testing of disinfectants to assess their effectiveness and safety."

The test results are shown in the table.

Research results

* The stability of the antiseptic disinfectant was studied for 4 months. The obtained samples of various compositions underwent 8 cycles of freezing and thawing. It was found that samples of composition 2 and 4 are more resistant to temperature changes (during thawing, the solution remained transparent without a change in pH. Mass fractions of active substances did not change.

In samples 1 and 5, during freezing, precipitation of crystals of potassium alum was observed.

* The effectiveness of an antiseptic disinfectant as a skin antiseptic is established for samples of composition 2 and 4. During hygienic treatment of hands, sanitization of the skin, when processing the hands of medical personnel, a micron protective layer forms on the surface, which completely blocks the skin or surfaces from pathogenic microorganisms and viruses. The protective layer lasts up to 4 hours, and bacteria, viruses, fungi that have got on it die. Such protection is completely safe for humans.

* The agent has bactericidal activity against gram-positive bacteria, including mycobacterium tuberculosis (tested for Mycobacterium terrae) and gram-negative bacteria (including causative agents of infections associated with the provision of medical care - HAI, intestinal infections); fungicidal activity (including fungi of the genus Candida, dermatophytes); antiviral activity (herpes, flu).

* The effectiveness of means for disinfecting surfaces is established by bactericidal, tuberculocidal, fungicidal and virucidal activity against test microorganisms.

The organoleptic control of the agent for use as a skin antiseptic showed: samples 2 and 4 are a clear liquid, there are no visible mechanical inclusions, a pleasant aromatic smell.

Thus, the developed agent has broad antimicrobial and antiviral activity as a skin antiseptic and disinfectant for small surfaces, stability of quality indicators, and improved organoleptic properties.

Table - Results of tests of an antiseptic preparation

Structure Distilled water
or demineralized Aluminum
potassium alum Cacao butter β-phenyl ethanol Propolis tincture 20% Lanolin Sodium chloride ε-
aminocaproic acid Protective layer retention time Resistant to temperature changes one 92.10 1.90 3.00 0.15 1.00 1.85 3.6 When frozen, crystals fall out 2 92,00 1.76 3.12 0.20 1.01 1.91 4.0 Without changes 3 91.00 1.80 3.20 0.25 1.10 2.65 4.0 Without changes 4 90.00 2.00 3.30 0.30 1.70 2.70 4.0 Without changes 5 89.00 2.60 3.50 0.30 2.00 2.60 4.0 When frozen, crystals fall out Patent 2501556 60.93-73.98 19.65-32.84 1.77-2.23 0.58-0.71 2.14-3.25 2.8 Crystallization at room temperature

Claims (2)

An antiseptic agent comprising a solution containing water, potassium alum, lanolin, characterized in that it additionally includes cocoa butter, β-phenyl ethanol, 20% aqueous propolis extract, in the following ratio of ingredients, wt%: distilled or demineralized water 90.00-92.00 potassium alum 1.76-2.00 cacao butter 3.12-3.30 β-phenyl ethanol 0.20-0.30 20% aqueous propolis extract 1.01-1.70 lanolin 1.91-2.70