RU2733887C2 - Мультиплексное детектирование нуклеиновых кислот - Google Patents

Мультиплексное детектирование нуклеиновых кислот Download PDF

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RU2733887C2
RU2733887C2 RU2016126375A RU2016126375A RU2733887C2 RU 2733887 C2 RU2733887 C2 RU 2733887C2 RU 2016126375 A RU2016126375 A RU 2016126375A RU 2016126375 A RU2016126375 A RU 2016126375A RU 2733887 C2 RU2733887 C2 RU 2733887C2
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sequence
probes
target
ligation
nucleic acid
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Карл Оскар Фредрик ДАХЛ
Джон Олоф ЭРИКССОН
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Ванадис Дайэгностикс
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RU2016126375A 2013-12-02 2014-11-26 Мультиплексное детектирование нуклеиновых кислот RU2733887C2 (ru)

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GB1321196.6 2013-12-02
GB1321196.6A GB2520765A (en) 2013-12-02 2013-12-02 Multiplex detection of nucleic acids
PCT/IB2014/003062 WO2015083002A2 (en) 2013-12-02 2014-11-26 Multiplex detection of nucleic acids

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EP (2) EP3077541B1 (enrdf_load_stackoverflow)
JP (2) JP6749236B2 (enrdf_load_stackoverflow)
KR (2) KR102307230B1 (enrdf_load_stackoverflow)
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AU (1) AU2014358862B2 (enrdf_load_stackoverflow)
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Families Citing this family (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4219745B1 (en) 2013-06-25 2025-09-03 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
GB2520763A (en) * 2013-12-02 2015-06-03 Vanadis Diagnostics Ab Nucleic acid probe and method of detecting genomic fragments
GB2520765A (en) 2013-12-02 2015-06-03 Vanadis Diagnostics Ab Multiplex detection of nucleic acids
WO2015189390A1 (en) 2014-06-13 2015-12-17 Q-Linea Ab Method for detecting and characterising a microorganism
ES2994053T3 (en) 2015-04-10 2025-01-17 10X Genomics Sweden Ab Spatially distinguished, multiplex nucleic acid analysis of biological specimens
GB201507026D0 (en) 2015-04-24 2015-06-10 Linea Ab Q Medical sample transportation container
GB201507376D0 (en) 2015-04-30 2015-06-17 Vanadis Diagnostics Ab Use of a porous transparent capillary membrane for counting rolling circle amplification products
CN111154754B (zh) * 2015-09-18 2024-02-02 苏州新波生物技术有限公司 分析dna样品的探针集合和使用所述探针集合的方法
GB2554767A (en) 2016-04-21 2018-04-11 Q Linea Ab Detecting and characterising a microorganism
CN110023505B (zh) 2016-10-27 2023-04-04 瓦纳迪斯诊断公司 用于处理滚环扩增产物的方法
GB201621514D0 (en) 2016-12-16 2017-02-01 Q-Linea Ab Padlock probe detection method
CN106929593B (zh) * 2017-04-27 2021-04-30 华侨大学 一种原位核酸检测方法
CN109252224A (zh) * 2017-07-14 2019-01-22 深圳华大基因股份有限公司 一种环状探针和基于环状探针捕获的测序文库构建方法
US11117128B2 (en) 2017-08-25 2021-09-14 Vanadis Diagnostics Ab Filtration device
AU2019247652B2 (en) 2018-04-02 2025-07-17 Enumera Molecular, Inc. Methods, systems, and compositions for counting nucleic acid molecules
US12203129B2 (en) * 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
SG11202105957YA (en) * 2018-12-05 2021-07-29 Egi Tech Shen Zhen Co Limited Rolling circle amplification method, method for preparing sequencing library, and dna nanosphere prepared therefrom
EP3894590A2 (en) 2018-12-10 2021-10-20 10X Genomics, Inc. Methods of using master / copy arrays for spatial detection
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
EP3947718A4 (en) 2019-04-02 2022-12-21 Enumera Molecular, Inc. METHODS, SYSTEMS AND COMPOSITIONS FOR COUNTING NUCLEIC ACID MOLECULES
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
CN112029841B (zh) * 2019-06-03 2024-02-09 香港中文大学 定量端粒长度和基因组基序的方法
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
WO2021133842A1 (en) 2019-12-23 2021-07-01 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
FI3891300T3 (fi) 2019-12-23 2023-05-10 10X Genomics Inc Menetelmät spatiaalista analyysiä varten rna-templatoitua ligaatiota käyttäen
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
CN115916999A (zh) 2020-04-22 2023-04-04 10X基因组学有限公司 用于使用靶向rna耗竭进行空间分析的方法
ES2989052T3 (es) 2020-05-22 2024-11-25 10X Genomics Inc Medición espacio-temporal simultánea de la expresión génica y la actividad celular
EP4153776B1 (en) 2020-05-22 2025-03-05 10X Genomics, Inc. Spatial analysis to detect sequence variants
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
ES2981265T3 (es) 2020-06-08 2024-10-08 10X Genomics Inc Métodos para determinar un margen quirúrgico y métodos de uso del mismo
US20230220474A1 (en) * 2020-06-10 2023-07-13 Bonadea Diagnostics, Llc Sequence conversion reaction
EP4172362B1 (en) 2020-06-25 2024-09-18 10X Genomics, Inc. Spatial analysis of dna methylation
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
WO2022061305A1 (en) 2020-09-21 2022-03-24 Progenity, Inc. Compositions and methods for isolation of cell-free dna
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4260291A4 (en) 2020-12-11 2024-11-06 Enumera Molecular, Inc. METHODS AND SYSTEMS FOR IMAGE PROCESSING
EP4121555A1 (en) 2020-12-21 2023-01-25 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4305196B1 (en) 2021-04-14 2025-04-02 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
EP4347879B1 (en) 2021-06-03 2025-02-19 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
EP4352252A1 (en) * 2021-07-13 2024-04-17 10X Genomics, Inc. Methods for spatial analysis using targeted probe silencing
EP4509614A3 (en) 2021-09-01 2025-05-14 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
CN113801923A (zh) * 2021-10-20 2021-12-17 南京鼓楼医院 一种可直接用于血清标志物检测的数字滚环扩增检测方法
EP4419707A1 (en) 2021-11-10 2024-08-28 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
CN116625999B (zh) * 2023-05-17 2023-11-28 中国科学院苏州生物医学工程技术研究所 一种评估零模波导孔有效载样的方法及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011142836A2 (en) * 2010-05-14 2011-11-17 Fluidigm Corporation Assays for the detection of genotype, mutations, and/or aneuploidy
WO2012019200A2 (en) * 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Assay systems for determination of source contribution in a sample
RU2478716C2 (ru) * 2005-07-04 2013-04-10 Эрасмус Юниверсити Медикал Сентр Улавливание и характеристика совместно локализованного хроматина

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE9400522D0 (sv) 1994-02-16 1994-02-16 Ulf Landegren Method and reagent for detecting specific nucleotide sequences
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
EP1997909B1 (en) 1998-03-25 2012-02-01 Olink AB Rolling circle replication of circularised target nucleic acid fragments
WO2000004193A1 (en) * 1998-07-20 2000-01-27 Yale University Method for detecting nucleic acids using target-mediated ligation of bipartite primers
WO2001094625A2 (en) 2000-06-06 2001-12-13 Tm Bioscience Corporation Capture moieties for nucleic acids and uses thereof
CA2426824A1 (en) 2000-10-24 2002-07-25 The Board Of Trustees Of The Leland Stanford Junior University Direct multiplex characterization of genomic dna
GB2378245A (en) 2001-08-03 2003-02-05 Mats Nilsson Nucleic acid amplification method
US7208295B2 (en) 2001-11-19 2007-04-24 Affymetrix, Inc. Multiplex oligonucleotide addition and target amplification
US20050164205A1 (en) * 2002-11-19 2005-07-28 Singulex, Inc. Charge and mass tags for detection and analysis
EP2204458A1 (en) 2003-11-04 2010-07-07 Applied Biosystems, LLC Concatameric ligation products: compositions, methods and kits for same
SE0401270D0 (sv) 2004-05-18 2004-05-18 Fredrik Dahl Method for amplifying specific nucleic acids in parallel
US20070087355A1 (en) 2005-10-14 2007-04-19 Barrett Michael T Comparative genomic hybridization assays and compositions for practicing the same
US8038595B2 (en) 2006-01-25 2011-10-18 Beth Israel Deaconess Medical Center Devices and methods for tissue transplant and regeneration
US8293501B2 (en) 2006-09-12 2012-10-23 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for performing low background multiplex nucleic acid amplification reactions
WO2008153492A1 (en) 2007-06-11 2008-12-18 Henrik Johansson Method for introducing common and/or individual sequence elements in a target nucleic acid molecule
US20090061424A1 (en) 2007-08-30 2009-03-05 Sigma-Aldrich Company Universal ligation array for analyzing gene expression or genomic variations
GB0912909D0 (en) 2009-07-23 2009-08-26 Olink Genomics Ab Probes for specific analysis of nucleic acids
WO2011068909A2 (en) * 2009-12-01 2011-06-09 Brigham And Women's Hospital, Inc. Aptamer cell compositions
GB0921264D0 (en) 2009-12-03 2010-01-20 Olink Genomics Ab Method for amplification of target nucleic acid
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
GB2492042B (en) 2011-05-11 2020-04-15 Agilent Technologies Inc Selector probes for nucleic acid analysis comprising at each end a non-adjacent target specific region
US20140336058A1 (en) 2011-12-02 2014-11-13 Qiagen Gmbh Method and kit for characterizing rna in a composition
CA2861856C (en) 2012-01-20 2020-06-02 Sequenom, Inc. Diagnostic processes that factor experimental conditions
US9892230B2 (en) 2012-03-08 2018-02-13 The Chinese University Of Hong Kong Size-based analysis of fetal or tumor DNA fraction in plasma
US20130267425A1 (en) 2012-04-06 2013-10-10 The Chinese University Of Hong Kong Noninvasive prenatal diagnosis of fetal trisomy by allelic ratio analysis using targeted massively parallel sequencing
EP2653559A1 (en) 2012-04-20 2013-10-23 Samsung Electronics Co., Ltd Polynucleotide and use thereof
CA2901138A1 (en) 2013-03-12 2014-10-09 Counsyl, Inc. Systems and methods for prenatal genetic analysis
GB2520765A (en) 2013-12-02 2015-06-03 Vanadis Diagnostics Ab Multiplex detection of nucleic acids
GB2520763A (en) 2013-12-02 2015-06-03 Vanadis Diagnostics Ab Nucleic acid probe and method of detecting genomic fragments
US10174383B2 (en) * 2014-08-13 2019-01-08 Vanadis Diagnostics Method of estimating the amount of a methylated locus in a sample

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2478716C2 (ru) * 2005-07-04 2013-04-10 Эрасмус Юниверсити Медикал Сентр Улавливание и характеристика совместно локализованного хроматина
WO2011142836A2 (en) * 2010-05-14 2011-11-17 Fluidigm Corporation Assays for the detection of genotype, mutations, and/or aneuploidy
WO2012019200A2 (en) * 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Assay systems for determination of source contribution in a sample

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