RU2721855C2 - Не являющиеся человеком животные с дефицитом дпнрнк - Google Patents

Не являющиеся человеком животные с дефицитом дпнрнк Download PDF

Info

Publication number
RU2721855C2
RU2721855C2 RU2016107434A RU2016107434A RU2721855C2 RU 2721855 C2 RU2721855 C2 RU 2721855C2 RU 2016107434 A RU2016107434 A RU 2016107434A RU 2016107434 A RU2016107434 A RU 2016107434A RU 2721855 C2 RU2721855 C2 RU 2721855C2
Authority
RU
Russia
Prior art keywords
dpnrna
rodent
locus
nucleic acid
expression
Prior art date
Application number
RU2016107434A
Other languages
English (en)
Russian (ru)
Other versions
RU2016107434A (ru
Inventor
Ка-ман ЛАЙ
Гуочун ГОНГ
Джон РИНН
Дэйвид ФРЕНДЕУЭЙ
Дэйвид М. ВАЛЕНСУЭЛА
Original Assignee
Регенерон Фармасьютикалз, Инк.
Президент Энд Феллоуз Оф Харвард Колледж
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Регенерон Фармасьютикалз, Инк., Президент Энд Феллоуз Оф Харвард Колледж filed Critical Регенерон Фармасьютикалз, Инк.
Publication of RU2016107434A publication Critical patent/RU2016107434A/ru
Application granted granted Critical
Publication of RU2721855C2 publication Critical patent/RU2721855C2/ru

Links

Images

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027New or modified breeds of vertebrates
    • A01K67/0275Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276Knock-out vertebrates
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61DVETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00Instruments or methods for reproduction or fertilisation
    • A61D19/04Instruments or methods for reproduction or fertilisation for embryo transplantation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0606Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0696Artificially induced pluripotent stem cells, e.g. iPS
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00Genetically modified animals
    • A01K2217/07Animals genetically altered by homologous recombination
    • A01K2217/075Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00Genetically modified animals
    • A01K2217/07Animals genetically altered by homologous recombination
    • A01K2217/075Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A01K2217/077Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out heterozygous knock out animals displaying phenotype
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00Animals characterised by species
    • A01K2227/10Mammal
    • A01K2227/105Murine
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00Animals characterised by purpose
    • A01K2267/03Animal model, e.g. for test or diseases
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00Animals characterised by purpose
    • A01K2267/03Animal model, e.g. for test or diseases
    • A01K2267/0393Animal model comprising a reporter system for screening tests
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/178Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Veterinary Medicine (AREA)
  • General Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Animal Behavior & Ethology (AREA)
  • Reproductive Health (AREA)
  • Developmental Biology & Embryology (AREA)
  • Analytical Chemistry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Transplantation (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
RU2016107434A 2013-08-07 2014-08-07 Не являющиеся человеком животные с дефицитом дпнрнк RU2721855C2 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361863147P 2013-08-07 2013-08-07
US61/863,147 2013-08-07
PCT/US2014/050178 WO2015021298A2 (en) 2013-08-07 2014-08-07 Lincrna-deficient non-human animals

Publications (2)

Publication Number Publication Date
RU2016107434A RU2016107434A (ru) 2017-09-15
RU2721855C2 true RU2721855C2 (ru) 2020-05-25

Family

ID=52449819

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2016107434A RU2721855C2 (ru) 2013-08-07 2014-08-07 Не являющиеся человеком животные с дефицитом дпнрнк

Country Status (19)

Country Link
US (3) US9554564B2 (OSRAM)
EP (2) EP3178928B1 (OSRAM)
JP (6) JP6998656B2 (OSRAM)
KR (3) KR102138723B1 (OSRAM)
CN (2) CN105518132B (OSRAM)
AU (3) AU2014305873B2 (OSRAM)
BR (1) BR112016002385A2 (OSRAM)
CA (1) CA2917714A1 (OSRAM)
DK (1) DK3178928T3 (OSRAM)
ES (1) ES2812242T3 (OSRAM)
HK (1) HK1220990A1 (OSRAM)
IL (2) IL243581B (OSRAM)
MX (3) MX369452B (OSRAM)
MY (2) MY182065A (OSRAM)
NZ (1) NZ716168A (OSRAM)
RU (1) RU2721855C2 (OSRAM)
SG (2) SG11201600036QA (OSRAM)
WO (1) WO2015021298A2 (OSRAM)
ZA (1) ZA201600170B (OSRAM)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018157058A1 (en) 2017-02-27 2018-08-30 Regeneron Pharmaceuticals, Inc. Non-human animal models of retinoschisis
WO2019018410A1 (en) * 2017-07-17 2019-01-24 The General Hospital Corporation COMPOSITIONS AND METHODS FOR TREATING INFLAMMATORY BOWEL DISEASES
CN120193018A (zh) 2017-09-19 2025-06-24 热带生物科学英国有限公司 修饰在真核细胞中用于沉默基因表达的非编码rna分子的特异性的方法
US11932858B2 (en) 2017-12-14 2024-03-19 Donald Danforth Plant Science Center Homologous recombination via transcriptional activation
CN108728545A (zh) * 2018-06-25 2018-11-02 汕头大学医学院附属肿瘤医院 结直肠癌长链非编码rna-hotair分子标志物及其应用
CN108866198B (zh) * 2018-08-31 2020-08-11 青岛泱深生物医药有限公司 标志物loc105376380在直肠腺癌诊断和治疗中的应用
CN108950000B (zh) * 2018-08-31 2020-08-04 青岛泱深生物医药有限公司 lncRNA在直肠腺癌诊疗中的应用
CN108950001B (zh) * 2018-08-31 2020-08-11 青岛泱深生物医药有限公司 一种与直肠腺癌发生发展相关的分子标志物
CN111218451B (zh) * 2020-02-05 2021-08-10 华中农业大学 一种提高猪肌肉量的方法
RU2752663C1 (ru) * 2020-05-18 2021-07-29 ОБЩЕСТВО С ОГРАНИЧЕННОЙ ОТВЕТСТВЕННОСТЬЮ "СберМедИИ" Способ квантификации статистического анализа альтернативного сплайсинга в данных рнк-сек
JPWO2022138421A1 (OSRAM) * 2020-12-22 2022-06-30
USD1010297S1 (en) * 2021-06-30 2024-01-09 Puma SE Shoe
CN113999873B (zh) * 2021-12-31 2022-05-20 北京市疾病预防控制中心 一种基因修饰的非人动物的构建方法及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110104799A1 (en) * 2009-10-29 2011-05-05 Regeneron Pharmaceuticals, Inc. Multifunctional Alleles
RU2425880C2 (ru) * 2009-07-30 2011-08-10 Учреждение Российской академии наук Институт общей генетики им. Н.И. Вавилова РАН Способ получения трансгенных мышей

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6586251B2 (en) 2000-10-31 2003-07-01 Regeneron Pharmaceuticals, Inc. Methods of modifying eukaryotic cells
US7105348B2 (en) 2000-10-31 2006-09-12 Regeneron Pharmaceuticals, Inc. Methods of modifying eukaryotic cells
US6596541B2 (en) 2000-10-31 2003-07-22 Regeneron Pharmaceuticals, Inc. Methods of modifying eukaryotic cells
PT2767161T (pt) 2004-10-19 2018-04-20 Regeneron Pharma Método para gerar um animal homozigótico para uma modificação genética
CN101633923B (zh) * 2009-07-10 2011-04-13 四川大学 人黑色素瘤细胞相关的长非编码rna及其用途
CN101619312B (zh) 2009-07-10 2011-04-20 四川大学 人黑色素瘤细胞特异表达的长非编码rna序列及其用途
WO2011020005A1 (en) 2009-08-14 2011-02-17 Regeneron Pharmaceuticals, Inc. miRNA-REGULATED DIFFERENTIATION-DEPENDENT SELF-DELETING CASSETTE
US20120004278A1 (en) 2010-06-18 2012-01-05 The Board Of Trustees Of The Leland Stanford Junior University Linc rnas in cancer diagnosis and treatment
WO2012018881A2 (en) 2010-08-03 2012-02-09 Alnylam Pharmaceuticals, Inc. Methods and compositions for the regulation of rna
WO2013134558A1 (en) 2012-03-07 2013-09-12 The Texas A & M University System Cancer treatment targeting non-coding rna overexpression
EP2958990B1 (en) 2013-02-20 2019-10-16 Regeneron Pharmaceuticals, Inc. Genetic modification of rats
RS62263B1 (sr) 2013-04-16 2021-09-30 Regeneron Pharma Ciljana modifikacija genoma pacova
ES2955841T3 (es) * 2015-03-16 2023-12-07 Regeneron Pharma Célula de un roedor que presenta función de la neurona motora superior e inferior y percepción sensorial disminuidas

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2425880C2 (ru) * 2009-07-30 2011-08-10 Учреждение Российской академии наук Институт общей генетики им. Н.И. Вавилова РАН Способ получения трансгенных мышей
US20110104799A1 (en) * 2009-10-29 2011-05-05 Regeneron Pharmaceuticals, Inc. Multifunctional Alleles

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
MONTSERRAT C. ANGUERA et al., Tsx Produces a Long Noncoding RNA and Has General Functions in the Germline, Stem Cells, and Brain, PLoS Genetics, 2011, Vol. 7, Issue 9, e1002248. *
YUNLI ZHOU et al., MEG3 noncoding RNA: a tumor suppressor, J Mol Endocrinol, 2012, Vol.48, R45-R53. FRANCESCA E. GORDON et al., Increased *
YUNLI ZHOU et al., MEG3 noncoding RNA: a tumor suppressor, J Mol Endocrinol, 2012, Vol.48, R45-R53. FRANCESCA E. GORDON et al., Increased Expression of Angiogenic Genes in the Brains of Mouse Meg3-Null Embryos, Endocrinology, 2010, Vol.151, N.6, pp.2443-2452. *

Also Published As

Publication number Publication date
US11547100B2 (en) 2023-01-10
CN105518132A (zh) 2016-04-20
DK3178928T3 (da) 2020-08-24
AU2014305873B2 (en) 2017-08-31
EP3030653A2 (en) 2016-06-15
AU2018200281A1 (en) 2018-02-01
KR20160035585A (ko) 2016-03-31
NZ716168A (en) 2022-05-27
JP2019037253A (ja) 2019-03-14
SG11201600036QA (en) 2016-02-26
JP2016526924A (ja) 2016-09-08
CA2917714A1 (en) 2015-02-12
US20230189771A1 (en) 2023-06-22
KR101853466B1 (ko) 2018-04-30
SG10201803766UA (en) 2018-06-28
BR112016002385A2 (pt) 2017-09-12
AU2017200183A1 (en) 2017-02-02
WO2015021298A3 (en) 2015-04-02
IL243581B (en) 2022-08-01
MY182065A (en) 2021-01-18
AU2014305873A1 (en) 2016-02-04
KR20200090977A (ko) 2020-07-29
JP6192865B2 (ja) 2017-09-06
MX2022013409A (es) 2022-11-14
EP3178928B1 (en) 2020-06-10
AU2018200281B2 (en) 2020-10-08
IL243581A0 (en) 2016-03-31
CN112715485A (zh) 2021-04-30
MX2016001682A (es) 2017-04-13
JP2020108409A (ja) 2020-07-16
JP6998656B2 (ja) 2022-02-04
KR102138723B1 (ko) 2020-07-29
JP2024164230A (ja) 2024-11-26
AU2017200183C1 (en) 2023-04-27
KR102350200B1 (ko) 2022-01-14
ZA201600170B (en) 2022-03-30
ES2812242T3 (es) 2021-03-16
RU2016107434A (ru) 2017-09-15
HK1220990A1 (zh) 2017-05-19
US20170071173A1 (en) 2017-03-16
AU2017200183B2 (en) 2018-02-01
MX369452B (es) 2019-11-08
US9554564B2 (en) 2017-01-31
IL294443A (en) 2022-09-01
KR20170012567A (ko) 2017-02-02
MY198884A (en) 2023-10-02
EP3030653A4 (en) 2017-07-26
JP2017099407A (ja) 2017-06-08
MX2019013309A (es) 2020-07-28
US20150047062A1 (en) 2015-02-12
WO2015021298A2 (en) 2015-02-12
EP3178928A1 (en) 2017-06-14
CN105518132B (zh) 2021-02-05
JP2022125255A (ja) 2022-08-26

Similar Documents

Publication Publication Date Title
RU2721855C2 (ru) Не являющиеся человеком животные с дефицитом дпнрнк
NZ716168A9 (en) Lincrna-deficient non-human animals
JP2022048157A (ja) 上位及び下位運動ニューロン機能並びに知覚の減衰を示す非ヒト動物
HK1238673B (en) Pint knockout mouse exhibiting a premature aging-associated phenotype
HK1238673A1 (en) Pint knockout mouse exhibiting a premature aging-associated phenotype
HK1249360B (en) Non-human animal exhibiting diminished upper and lower motor neuron function and sensory perception