RU2679500C2 - Популяция стволовых клеток шейки матки человека и ее применение - Google Patents
Популяция стволовых клеток шейки матки человека и ее применение Download PDFInfo
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- RU2679500C2 RU2679500C2 RU2015139970A RU2015139970A RU2679500C2 RU 2679500 C2 RU2679500 C2 RU 2679500C2 RU 2015139970 A RU2015139970 A RU 2015139970A RU 2015139970 A RU2015139970 A RU 2015139970A RU 2679500 C2 RU2679500 C2 RU 2679500C2
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| EP135156348.8 | 2013-02-22 | ||
| PCT/EP2014/053508 WO2014128291A1 (en) | 2013-02-22 | 2014-02-24 | Human uterine cervical stem cell population and uses thereof |
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| JP2017529101A (ja) * | 2014-09-18 | 2017-10-05 | ナショナル ヘルス リサーチ インスティテュートス | 前駆細胞、それらの調製方法およびそれらの使用 |
| WO2018021805A1 (ko) * | 2016-07-25 | 2018-02-01 | 고려대학교 산학협력단 | 자궁 유래 성체줄기세포 분리 및 제조 방법 |
| CA3053887A1 (en) * | 2017-02-16 | 2018-08-23 | OCT Therapies and Research Private Limited | Stem cell conditioned media for clinical and cosmetic applications |
| CN108865973A (zh) * | 2018-06-20 | 2018-11-23 | 广西医科大学第附属医院 | Sd大鼠胃窦间质细胞的分离和培养方法 |
| WO2021226373A2 (en) * | 2020-05-08 | 2021-11-11 | Gallant Pet, Inc. | Uterine-derived regenerative cell compositions and uses thereof |
| CN112057471B (zh) * | 2020-09-11 | 2023-12-22 | 复旦大学附属妇产科医院 | 间充质干细胞的制药用途 |
| CN112618712A (zh) * | 2021-01-21 | 2021-04-09 | 武汉轻工大学 | 一种含波形蛋白的佐剂及应用 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011042547A1 (en) * | 2009-10-08 | 2011-04-14 | Projech Science To Technology, S.L. | Myometrial-derived mesenchymal stem cells and uses thereof |
| CN102229911A (zh) * | 2011-06-08 | 2011-11-02 | 山西医科大学 | Sca-1+/CD34－子宫干细胞及其分离方法 |
| RU2481585C1 (ru) * | 2011-11-09 | 2013-05-10 | Федеральное государственное бюджетное учреждение "Московский научно-исследовательский онкологический институт им. П.А. Герцена Министерства здравоохранения и социального развития Российской Федерации" (ФГБУ "МНИОИ им. П.А. Герцена" Минздравсоцразвития России) | Способ дифференциальной диагностики лейомиосарком и пролиферирующих (клеточных) лейомиом матки |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011042547A1 (en) * | 2009-10-08 | 2011-04-14 | Projech Science To Technology, S.L. | Myometrial-derived mesenchymal stem cells and uses thereof |
| CN102229911A (zh) * | 2011-06-08 | 2011-11-02 | 山西医科大学 | Sca-1+/CD34－子宫干细胞及其分离方法 |
| RU2481585C1 (ru) * | 2011-11-09 | 2013-05-10 | Федеральное государственное бюджетное учреждение "Московский научно-исследовательский онкологический институт им. П.А. Герцена Министерства здравоохранения и социального развития Российской Федерации" (ФГБУ "МНИОИ им. П.А. Герцена" Минздравсоцразвития России) | Способ дифференциальной диагностики лейомиосарком и пролиферирующих (клеточных) лейомиом матки |
Non-Patent Citations (2)
| Title |
|---|
| FIGUEIRA P.G.M., et al. "Stem Cells in Endometrium and Their Role in the Pathogenesis of Endometriosis", Ann N Y Acad Sci. 2011; 1221(1):10-17. * |
| SUN X., et al. " Mesenchymal stem cells isolated from human uterine cervix cancer tissues", Cell Biol Int. 2011; 35(2):119-123. * |
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