RU2676326C1 - Complex antibacterial antihelminthic composition for treatment of farm animals and birds - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: invention relates to medicine, namely to veterinary medicine, and is intended for the treatment of diseases of mixed bacterial and helminth etiology in farm animals and birds. Complex antibacterial antihelminthic composition contains azithromycin, praziquantel, fenbendazol, bentonite, iodized table salt, molasses, chelated copper and blue vitriol. Components are used in the stated quantities.

EFFECT: use of invention improves efficiency of treatment of diseases of mixed bacterial and helminth etiology before isolating the causative agent and studying its sensitivity to antibiotics, reduce the time and reduce the complexity of treatment.

1 cl, 4 ex, 2 tbl

Description

The invention relates to the field of veterinary medicine and can be used for the treatment and prevention of diseases of mixed bacterial and helminthiasis etiology in farm animals.

Diseases of mixed bacterial and helminthiasis etiology in acute or subacute course significantly increase animal waste, cause low weight gain, increased sensitivity to stress, worsen the productivity and biological quality of meat and milk, reduce post-vaccination immunity, poor bioconversion of feed (helminthiasis + pasteurellosis, helminthiasis + colibacteriosis, helminth infections + staphylococcosis, helminth infections + streptococcoses, helminth infections + mycoplasmoses, helminth infections + giardiasis and other combinations).

The study of the species composition of the microflora and fauna of helminths and their pathogenic properties isolated from healthy, sick or dead animals indicates its polymicrobial composition and the diversity of the species of helminth fauna in the most diverse associations and combinations.

The reason for the gastrointestinal diseases of farm animals in recent years is the mixed nature of microflora and helminth fauna, which complicates the course of diseases and the choice of an effective drug, as well as the increasing resistance of pathogens.

Thus, the widespread spread of diseases of mixed bacterial and helminthic etiology cause enormous damage to agricultural production and hinder the development of livestock production, is one of the reasons for the deterioration of the epizootic situation, the decrease in productivity and breeding qualities of animals, their forced slaughter and death, and requires high costs for treatment and prevention, to develop new effective means of combating associative diseases of farm animals.

The most appropriate method that increases the effectiveness of treatment of diseases of mixed bacterial and helminthiasis etiology and slows down the development of resistance in pathogens is the rational use of combinations of various antibiotics and anthelmintics, which have a wide spectrum of antimicrobial and antiparasitic effects by oral methods.

Among the well-known complex drugs for the treatment of associative diseases of farm animals, a mixed bacterial and helminthiological etiology of powder preparations intended for individual and group therapy and prevention by oral methods has not been developed.

A complex drug is known for the treatment of bacterial diseases of cattle based on antibiotics of the latest generations with a wide spectrum of bacteriostatic action - doxycycline and florfenicol. The drug is administered intramuscularly. The use of a new drug based on doxycycline and florfenicol in diseases of young cattle is highly effective in tracheitis and dyspepsia of young cattle, in the doses used it does not have hepato- and nephrotoxicity [1, 2].

However, in the specified source of information there is no information about the possibility of using the known drug for other types of farm animals and birds, and the intramuscular route of administration of the drug increases the complexity of the treatment method.

Known antibacterial composition for the treatment of farm animals and birds, containing a compound from the group of fluoroquinolones and apramycin sulfate in the following ratio (in parts by weight): compound from the group of fluoroquinolones: apramycin sulfate 1: 2-1: 10 [2, 3, 6] .

The antibacterial pharmaceutical composition as a compound from the group of fluoroquinolones contains enrofloxacin, or ciprofloxacin, or norfloxacin, or pefloxacin, or ofloxacin, or levofloxacin, or moxifloxacin. An antibacterial pharmaceutical composition for treating farm animals and birds is only a complex antibacterial drug, but is not effective against helminths. Compounds from the group of fluoroquinolones included in the composition of the composition have a wide spectrum of antibacterial and antimycoplasmic effects. Active against gram-positive and gram-negative bacteria, not active against anaerobic microorganisms. The mechanism of action of compounds from the group of fluoroquinolones is based on the ability to inhibit the activity of the gyrase enzyme that ensures DNA replication in a bacterial cell [1, 2, 3, 6].

There are known preparations containing an avermectin complex as an active substance (AI), and drugs that are chemically modified members of the avermectin family, obtained on the basis of the soil fungus Streptomyces avermitilis (abictin, avertin, aversect-2, bimec, bimectin, dectomax, duotin, ivermag Ivermek, Iversect, Ivertin, Novomek), which are comprehensively studied and widely used in the fight against helminths [1, 2, 3].

However, the disadvantages of preparations containing avermectins or ivermectin as a DV are a high content of DV (1%) in the finished form and, accordingly, in a therapeutic dose of 20-30 mg per 100 kg of animal body weight [4], as well as the fact that they are inconvenient for use by small pets, such as small dogs and cats, due to the difficulty of dosing. When using ivermectin, care must be taken, since it has a hepatotoxic effect. It was established that dogs of the Collie, Border Collie, Bobtail, Shelty breed are especially sensitive to the action of ivermectin. The effect of ivermectin on the central nervous system of dogs was noted; in bracheocephalus (short-faced), the drug passes through the blood-brain barrier.

The DIRONET preparation for the prevention and treatment of animal helminthiasis, containing a compound from the group of avermectins, pyrantel or its salt and the target additive, contains praziquantel in the following ratio of ingredients, wt. h.: compound from the group of avermectins: pyrantel or its salt: praziquantel: target additive = 1.2: 3.0: 1.0: 6.0 [4].

As a compound from the group of avermectins, the preparation may contain ivermectin, or abamectin, or doramectin, or moxidectin.

The preparation as a pyrantel salt may contain pyrantel pamoate, or pyrantel tartrate, or pyrantel embonate, or pyrantel hydrochloride.

As a target additive, the preparation contains a mixture consisting of ascorbic acid, sodium bicarbonate, starch, sugar, aerosil, talc, sodium lauryl sulfate and polyvinylpyrrolidone.

The technical result provided by the invention is to increase the resistance of animals to reinfestation.

The drug is obtained by mixing or dissolving the components, and then granules, tablets, suspension, spot on or pur on are made [4].

The invention of dirofen includes albendazole sulfoxide (n- [6- (propan-1-sulfinyl) -1H-1,3-benzodiazol-2-yl] methoxycarboximidic acid) 5.0-10.0% by mass fraction of the ratio of components, butafosfan (1 -butylamino-1-methyl) ethylphosphonic acid) 10.0-20.0% of the mass fraction, water for injection - the rest and has the following advantages: 1. Complex therapy of side effects of helminth infections, due to the use of butafosfan as part of the preparation. 2. Low toxicity. 3. A convenient way of dosing and administration. The drug for the treatment of helminthiases in animals, containing albendazole sulfoxide, the accompanying substance additionally contains butafosfan. However, the disadvantage of the drug is its negative effect on the biochemical parameters of blood in case of an overdose. A study of the main biochemical parameters of blood serum revealed changes in the function of the liver, kidneys, protein metabolism, and enzyme activity under the action of the drug [5].

The objective of the present invention is to develop an effective and harmless comprehensive drug based on antibiotics and anthelmintics for the treatment of diseases of mixed bacterial and helminthiasis etiology in farm animals and birds.

The problem is solved in that a complex antibacterial pharmaceutical composition for treating farm animals and birds, containing azithromycin, praziquantel, fenbendazole, is characterized by the fact that it additionally contains auxiliary substances: bentonite (of the Hergepezh deposit (humidity 10%), iodized table salt, molasses, chelate copper, copper sulfate with a ratio in mg / g of powder: azithromycin - 100 mg, praziquantel - 100 mg, fenbendazole - 100 mg, bentonite- (Herpegezh deposit (humidity 10%) - 2 00 mg, iodized sodium chloride - 100 mg, molasses - 200 mg, chelated copper - 100 mg and copper sulfate - 100 mg.

The technical result of the claimed invention is that the obtained antibacterial-anthelmintic pharmaceutical composition for treating farm animals and birds has increased treatment efficiency with a reduction in the dose of active substances, allows not only treatment of mixed infection-invasions prior to isolation of etiotropic pathogens and study of their sensitivity to antibiotics and anthelmintic, but also reduce the time and reduce the complexity of treatment, and at the same time it is harmless and positive but it affects the morphology of the blood of animals and birds.

The claimed invention is characterized by the following examples.

Example 1. Obtaining an antibacterial-anthelmintic pharmaceutical composition.

Azithromycin was used as a bactericidal compound from the macrolide group, and praziquantel and fenbendazole substances were used as anthelmintics. and as auxiliary substances - bentonite (Herpegezh deposit (humidity 10%), iodized sodium chloride, molasses, chelated copper and copper sulfate. An antibacterial-anthelmintic pharmaceutical composition is obtained by mixing all the ingredients in a granulator mixer at 300 rpm for 30 minutes .

In appearance, the antibacterial-anthelmintic pharmaceutical composition is a gray powder and contains (in mg / 1 g of powder): azithromycin 100 mg, praziquantel 100 mg, fenbendazole 100 mg, bentonite of the Herpegezh deposit (humidity 10%) 200 mg, iodized table salt 100 mg, molasses 200 mg, chelated copper 100 mg and copper sulfate 100 mg. The claimed antibacterial-anthelmintic pharmaceutical composition for treating farm animals and birds is a complex antibacterial-anthelmintic oznym drug for the individual and group appointments. Included in its composition, azithromycin has a wide spectrum of antibacterial and antimycoplasmic effects. Active against gram-positive bacteria, including: Streptococcus pneumoniae, Streptococcus pyogenes, Staphylococcus aureus; aerobic gram-negative bacteria: Pasteurella multocida, Haemophilus influenzae, Haemophilus parainfluenzae, Moraxella catarrhalis, Neisseria gonorrhoeae, Legionella pneumophila; some anaerobic microorganisms: Fusobacterium spp., Clostridium perfringens, Prevotella spp., Porphyriomonas spp .; and also against Chlamydia psittaci, Chlamydia pneumoniae, Chlamydia trachomatis, Mycoplasma hominis, Mycoplasma pneumoniae, Borrelia burgdorferi.

The mechanism of action of azithromycin is associated with the suppression of protein synthesis of microbial cells. By binding to the 50S subunit of ribosomes, it inhibits the peptide translocase at the translation stage, inhibits protein synthesis, and slows the growth and reproduction of bacteria, i.e. acts bacteriostatically. In high concentrations it has a bactericidal effect.

Its component praziquantel has a wide spectrum of anthelmintic action in relation to bio - and geohelminths: Fasciola hepatica L., 1758; Dicrocoelium lanceatum Stilles et Hassall, 1896; Moniezia expansa Rud., 1810; Moniezia benedeni Moniez, 1879; Avitellina centripunctata Rivolta, 1874; Thysaniezia giardi Moniez, 1879; Echinococcus granulosus Batsch, 1789; Cysticercus tenuicollis Pallas, 1766 larvae; Ostertagia ostertagi Stilles, 1892; Ostertagia circumcincta; Ostertagia occidentalis; Ostertagia trifurcata; Ostertagia orloffi; Ostertagia trifida; Bunostomum trigonocephalum Rud., 1808; Bunostomum phlebotomum Railliet, 1900; Oesophagostomum radiatum Rud., 1803; Trichostrongylus axei Cob., 1879; Trichostrongylus capricola ransom, 1907; Trichostrongylus columbriformis Giles, 1829; Trichostrongylus skrjabini Kalant., 1928; Trichostrongylus vitrinus Looss, 1905; Haemonchus contortus Rud., 1803; Chabertia ovina Fabricius, 1788; C. oncophora railliet, 1898; Nematodirus filicollis Rud., 1802; Nematodirus helvetianus May, 1920; Nematodirus oiratianus; Nematodirus spathiger; Nematodirus abnormalis; Dictyocaulus filaria; Protostrongylus hobmaeri; Protostrongylus skrjabini; Protostrongylus raillieti; Mulleria capillaris; Cystocaulus nigrescens; Trichocephalus ovis; Trichocephalus skrjabini.

Its component fenbendazole also has a wide spectrum of anthelmintic action mainly in relation to geohelminths: Ostertagia ostertagi Stilles, 1892; Ostertagia circumcincta; Ostertagia occidentalis; Ostertagia trifurcata; Ostertagia orloffi; Ostertagia trifida; Chabertia ovina Fabricius, 1788; Bunostomum trigonocephalum Rud., 1808; Trichostrongylus axei Cob., 1879; Trichostrongylus capricola ransom, 1907; Trichostrongylus columbriformis Giles, 1829; Trichostrongylus skrjabini Kalant., 1928; Trichostrongylus vitrinus Looss, 1905; Bunostomum phlebotomum Railliet, 1900; Oesophagostomum radiatum; Haemonchus contortus Rud., 1803; Nematodirus helvetianus May, 1920; Nematodirus filicollis; Nematodirus oiratianus; Nematodirus spathiger; Nematodirus abnormalis; Nematodirus schulzi; Dictyocaulus filaria; Trichocephalus ovis; Trichocephalus skrjabini

The auxiliary substances bentonite (of the Herpegezh deposit (humidity 10%), iodized table salt, molasses, chelated copper and copper sulfate prolong the effect of azithromycin, praziquantel and fenbendazole, improve the palatability of the composition, and activate all types of metabolism.

Example 2. The study of acute toxicity of the composition.

Used the pharmaceutical composition obtained in example 1.

An experimental study of the acute toxicity of the composition was carried out on 45 male Balb / c mice with a live weight in the range of 22.0-25.0 grams. Clinically healthy animals were taken into the experiment, which were divided into 5 groups (9 goals in each) and kept on quarantine for 10 days and kept in the same conditions. In mice of 1-4 experimental groups, the composition was injected into the stomach in the form of an aqueous suspension in doses of 1, 3, 5 and 6 g / kg of body weight using a rubber probe. Control mice received water in the same volumes. The animals were clinically observed for 2 weeks after administration. We took into account the general condition of the animals, the preservation of motor functions, appetite, the condition of the coat, respiration, and reaction to external stimuli. In the blood of mice of these groups, some metabolic parameters were determined. The results obtained are summarized in table 1. From this table it can be seen that the composition helps to increase the total protein content in the blood serum of mice by 14-27%. Under the influence of the composition, the concentration of urea in the blood of mice increased, again most significantly in the third group. On average, the ACT activity in the blood serum of experimental animals was 2–8% lower than normal, and ALT, on the contrary, was 6–14% higher, which reflects its possible organostabilizing effect on ACT, as well as the normalization of liver resuscitation function. This is evidenced by the de Ritis coefficient (ACT / ALT), which fluctuated within normal limits. At the same time, after the introduction of the composition in the blood serum of male Balb / c mice of all experimental groups, the glucose content increased, on average, nV 8.5%. A positive point should be considered a decrease in cholesterol by 5.2-7.4% at all doses of the composition.

Thus, the claimed composition has a beneficial effect on mice. After administration of the drug, they show a tendency to improve the morphological picture and the biochemical spectrum of blood, there is a decrease in the manifestations of inflammation, general resistance increases, the metabolism and the functional state of the liver improve. In male Balb / c mice, hematology and hemostasis indicators, with the exception of the 4th group, were observed at the normal level (Table 2).

At the same time, the number of leukocytes in the peripheral blood of experimental animals statistically significantly increased, amounting to (7.48 ± 0.39) ⋅10 ⁹ l for the 1-, 2-, 3-, and 4 experimental groups, (7 , 95 ± 0.43) ⋅10 ⁹ l, (8.46 ± 0.52) ⋅10 ⁹ l, (9.82 ± 0.64) ⋅10 ⁹ l, with normal (7.21 ± 0.33 ) ⋅10 ⁹ l.

As a result of the introduction of the claimed composition in the dose range of 3-6 g / kg, the death of animals was not detected. The maximum tolerated dose of the drug in a single experiment should be considered a dose of 6 g / kg of body weight. The clinical picture of intoxication of male Balb / c mice is not expressed. All experimental animals were active, mobile, well-fed and practically did not differ from control animals. During postmortem autopsy, slight hyperemia of the gastric mucosa was observed in male Balb / c mice after a dose of 6 g / kg was administered. Pathanatomic changes in the liver, kidneys, heart, and spleen were not detected.

As can be seen, the claimed antibacterial-anthelmintic pharmaceutical composition for the treatment of farm animals and birds according to the classification of GOST 12.1.007-76 belongs to the 4th class of toxicity (low toxicity compound).

Example 3. The study of the cumulative properties of the composition in a subacute experiment.

To this end, 1/5 of the maximum dose (6 g / kg) was injected into the stomach of male Balb / c mice, i.e. the daily administered dose was 1.2 g / kg body weight. The duration of the experiment is 15 days. We took into account both material (death of animals) and functional cumulation.

The dose-effect relationship plot allowed the determination of lethal doses of LD ₁₆ and LD ₈₄ , which were 21.6 g / kg and 29.3 g / kg body weight, respectively.

The standard error LD _{50p of the} composition “Azitroprazifen” under repeated administration was determined by the Gaddam formula, which was 25.47 ± 1.62 g / kg body weight.

The cumulation coefficient (K _cum ) was calculated by the ratio of effective doses of subacute and acute experiments.

K _cum was 3.3, which, according to the classification of Yu.S. Kagan, allows you to attribute the claimed drug to the 3 hazard class (moderate cumulation).

In the study of functional cumulation, body weight was assessed, a sum-threshold indicator (SPP) was determined, peripheral blood analysis (erythrocytes, hemoglobin, leukocytes) was carried out, the functional state of the liver and kidneys was assessed, and some behavioral responses were recorded in experimental animals.

The functional state of the central nervous system was evaluated by the value of the sum-threshold indicator. The neuromuscular excitability of animals was determined using electrodes to reduce interdigital muscles with an increase in current supply.

To identify functional cumulation, animals were examined on days 5, 10, and 15 of the experiment (while the animals received total doses of 8, 16, and 24 g / kg, respectively). As follows from the results, in experimental animals, from the 10th day of the experiment, there is a tendency to decrease in body weight, and on the 15th day, after receiving a total dose of 24 g / kg, its decrease was noted.

At the same time, the sum-threshold indicator (SPP) significantly increased, which indicates the inhibition of animals.

The Celldyn hematology analyzer determined the status of peripheral blood and estimated the number of red blood cells, hemoglobin and white blood cells. In addition, various calculated values were used, reflecting the physicochemical properties of red blood cells, allowing quantitative characterization of their status indicators.

The research results indicate that from indicators characterizing the functional state of peripheral blood, a decrease in the amount of hemoglobin (P <0.05) on the 15th day was noted. All other blood indices in the experimental animals did not differ from the control and did not go beyond the physiological norm for these indices.

The results of measuring the rectal temperature and some behavioral reactions of male Balb / c mice indicate that their significant decrease was also noted on day 1.5. The remaining indicators of the experimental animals did not differ from the control and did not go beyond the physiological norm.

When studying the functional state of the kidneys, the specific gravity of urine, the frequency of urine output, the total protein in the urine and chlorides were determined.

The results of the studies showed that in experimental male Balb / c mice, all indicators characterizing the functional state of the kidneys do not differ from the indicators of control animals throughout the experiment.

The functional state of the liver was evaluated by the ability of the organ. synthesize hippuric acid (neutralizing function) and by the content of total protein in the blood serum.

Equally important for the life of the animal organism as a whole, as well as for evaluating the liver, is the determination of serum SH groups, which play an important role in the formation of intramolecular bonds; including -S-s-bonds, which, in the first place, provide rigid bonding of individual polypeptide chains in immunoglobulins.

As the results showed, in experimental male Balb / c mice at this time a significant decrease in urinary hippuric acid was revealed, which indicates a decrease in the neutralizing function of the liver. All other indicators characterizing the function of the liver in animals exposed to repeated exposure to the drug in a total dose of 24.0 g / kg did not significantly differ from the control values.

When registering behavioral reactions, indicators of dynamic activity and static performance of animals were taken into account.

Dynamic activity was determined using the “vertical” motor component, which is based on counting the number of animals rising on its hind legs in 3 minutes. There is evidence of a high sensitivity of this reaction for male Balb / c mice. The specified method can serve as an objective indicator of the general condition of animals.

To assess static muscle performance, the method of retaining male Balb / c mice on a horizontal rod was used. The duration of stay of the animal on the rod was taken into account. This method is the simplest and most affordable for toxicological studies. It does not require expensive devices, and the duration of its implementation can be taken into account with sufficient accuracy.

An analysis of the results of the experiment shows that the number of animals rising to their hind legs in 3 minutes decreases from the 10th to the 15th days of the experiment, and the duration of the animal’s stay on the rod also becomes shorter during this period of time.

After the end of the experiment, male Balb / c mice were killed and the mass coefficients of the internal organs were determined. The mass coefficients of organs in animals of the experimental group were on the same level as the control and did not differ significantly from it, which indicates that this indicator is not decisive when exposed to the composition.

Thus, under conditions of subchronic exposure, the claimed composition caused significant changes in the nervous system, the mass of male Balb / c mice, blood (decrease in hemoglobin) and a tendency to decrease behavioral indicators. This shows that the drug has negligible both material and functional cumulation. However, all changes were manifested only at the dose level, several times larger than practical, and in conditions of repeated administration. In connection with the foregoing, the drug does not pose a real danger in the treatment of farm animals and poultry.

Example 4

The effectiveness of the treatment of associative bacterial-helminth infections using the claimed composition.

The claimed antibacterial-anthelmintic pharmaceutical composition was investigated for therapeutic purposes on calves, foals and lambs for gastrointestinal diseases of bacterial helminthiasis etiology, including the associations of bio- and geohelminthiasis with pasteurellosis, colibacteriosis, salmonellosis and mycoplasma. Used the composition in a mixture with animal feed in a ratio of 1: 100, once. As a result of the experiments, it was found that the antibacterial-anthelmintic pharmaceutical composition has a pronounced antimicrobial and anthelmintic activity. After the composition was prescribed, the physiological and biochemical parameters of the blood returned to normal for 4-6 days. For example, in calves that received the claimed composition at a dose of 0.5-0.6 g / 10 kg of body weight, the general condition improved, and the clinical picture returned to normal 3-4 days after it was given. As a result of the tests, it was found that the following effective therapeutic doses of the composition (g / 10 kg of animal weight) are: calves and foals - 0.50, lambs - 0.30.

The study of morphobiochemical blood parameters of animals of experimental groups after application of the claimed composition indicates its good tolerance and pronounced biological effect on the animal organism. So, as a result of the use of the claimed drug for 5 days, the content of red blood cells, white blood cells and hemoglobin increased, the indicators of acid-base balance and total protein in the blood returned to normal. An increase in the number of red blood cells in the blood of experimental animals indicates an activation of redox processes and a decrease in toxic and inflammatory processes, as well as an improvement in the respiratory and toxic excretory functions of the body at the cellular level. The results on the change in the blood leukocyte content indicate an increase in phagocytic activity, activation of the body's defense reactions. An increase in the total protein in the blood in experimental animals indicates a normalization of the physiological state, activation of intracellular metabolism, and an increase in the level of nonspecific immune resistance, which indicates a positive effect of the pharmaceutical composition on the morphological parameters of the blood. An increase in the alkaline reserve of blood indicates a decrease in the processes of acidosis and a higher level of redox processes in the body. These positive changes occurring in the body, provide a reduction in treatment duration to 4-6 days, and also contribute to a more intensive growth of experimental animals.

Thus, a harmless and biosafe antibacterial-anthelmintic pharmaceutical composition is obtained for the complex treatment and prevention of associative diseases of farm animals and birds, which:

- has an increased therapeutic and prophylactic effect in mixed infectious invasions of bacterial and helminth infections, with a decrease in the dose of ADV (azithromycin, praziquantel and fenbendazole);

- positively affects the morphological parameters of blood;

- allows the treatment of mixed infectious infestations before the isolation of etiotropic pathogens and the study of their sensitivity to antibiotics and anthelmintics, but also reduce the time and reduce the complexity of treatment.

An antibacterial-anthelmintic pharmaceutical composition is obtained by mixing all the ingredients in a granulator mixer at 300 rpm. within 30 minutes.

The auxiliary substances that make up the composition of bentonite (Herpegezh deposit (humidity 10%), iodized sodium chloride, molasses, chelated copper and copper sulfate prolong the effect of azithromycin, praziquantel and fenbendazole, improve taste, activate metabolism. The following are effective therapeutic doses of the composition ( g / 10 kg of animal weight): calves and foals - 0.50, lambs - 0.30.

Information sources

1. The State Register of Medicines 2005. 8th ed., T. 2, M .: "Medicine", 2005, p. 1662-1663.

2. Mashkovsky M.D. Medicines / M.D. Mashkovsky. Ed. 15th M .: "New Wave", 2005 p. 803-804, 807-808, 847-848.

3. Encyclopedia of medicines, 12th ed., M .: LLC “RLS-2005”, 2004, p. 154-155.

4. Klenova I.F., Maltsev K.L., Yaremenko N.A., Arkhipov I.A. Veterinary drugs in Russia. M .: Selkhozizdat, 2004.Vol.

5. Patent No. 2571543 US "Drug for the treatment of animal helminthiasis."

6. Patent No. 2570389 US "Antibacterial pharmaceutical composition for the treatment of farm animals and birds."

Claims (2)

A complex antibacterial-anthelmintic composition for treating farm animals and birds, containing azithromycin, praziquantel, fenbendazole, characterized in that it additionally contains auxiliary substances: bentonite, iodized sodium chloride, molasses, chelated copper, copper sulfate in a ratio of mg / g of powder: azithromycin one hundred praziquantel one hundred fenbendazole one hundred bentonite 200 iodized salt one hundred syrup 200 chelated copper one hundred copper sulfate one hundred