RU2663587C2 - Клетки-хозяева и способы использования - Google Patents

Клетки-хозяева и способы использования Download PDF

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RU2663587C2
RU2663587C2 RU2015135980A RU2015135980A RU2663587C2 RU 2663587 C2 RU2663587 C2 RU 2663587C2 RU 2015135980 A RU2015135980 A RU 2015135980A RU 2015135980 A RU2015135980 A RU 2015135980A RU 2663587 C2 RU2663587 C2 RU 2663587C2
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host cell
nucleic acid
acid sequence
variant
cell according
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RU2015135980A
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RU2015135980A (ru
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Юнхвань ЦЗИНЬ
Юань ЧЖУ
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ГЛЭКСОСМИТКЛАЙН ЭлЭлСи
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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/04—Anorexiants; Antiobesity agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/04—Inotropic agents, i.e. stimulants of cardiac contraction; Drugs for heart failure
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0051—Oxidoreductases (1.) acting on a sulfur group of donors (1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
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    • C12Y—ENZYMES
    • C12Y108/00—Oxidoreductases acting on sulfur groups as donors (1.8)
    • C12Y108/04—Oxidoreductases acting on sulfur groups as donors (1.8) with a disulfide as acceptor (1.8.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21061—Kexin (3.4.21.61), i.e. proprotein convertase subtilisin/kexin type 9
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/04—Intramolecular oxidoreductases (5.3) transposing S-S bonds (5.3.4)
    • C12Y503/04001—Protein disulfide-isomerase (5.3.4.1), i.e. disufide bond-forming enzyme

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  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
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  • Microbiology (AREA)
  • Molecular Biology (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Diabetes (AREA)
  • Cardiology (AREA)
  • Obesity (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Neurosurgery (AREA)
  • Emergency Medicine (AREA)
  • Neurology (AREA)
  • Hospice & Palliative Care (AREA)
  • Virology (AREA)
RU2015135980A 2013-03-06 2014-03-06 Клетки-хозяева и способы использования RU2663587C2 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361773329P 2013-03-06 2013-03-06
US61/773,329 2013-03-06
PCT/US2014/021137 WO2014138371A1 (en) 2013-03-06 2014-03-06 Host cells and methods of use

Publications (2)

Publication Number Publication Date
RU2015135980A RU2015135980A (ru) 2017-04-10
RU2663587C2 true RU2663587C2 (ru) 2018-08-07

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US (1) US9926570B2 (enExample)
EP (1) EP2964666B1 (enExample)
JP (2) JP6742729B2 (enExample)
KR (1) KR20150126839A (enExample)
AU (1) AU2014225678B2 (enExample)
BR (1) BR112015021960A2 (enExample)
CA (1) CA2903739A1 (enExample)
ES (1) ES2843679T3 (enExample)
RU (1) RU2663587C2 (enExample)
SG (1) SG11201504771RA (enExample)
WO (1) WO2014138371A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX364532B (es) 2013-07-04 2019-04-29 Glykos Finland Oy Celulas de hongos filamentosos deficientes de o-manosiltransferasa y metodos de uso de las mismas.
CN108064266A (zh) 2014-07-21 2018-05-22 格利科斯芬兰公司 在丝状真菌中具有哺乳动物样n-聚糖的糖蛋白的制备
CN108431204A (zh) 2015-12-22 2018-08-21 阿尔布梅迪克斯医疗有限公司 改良的表达蛋白质的菌株
JP7146637B2 (ja) 2015-12-22 2022-10-04 アルブミディクス リミティド 改善されたタンパク質発現株
CN105950491B (zh) * 2016-05-23 2019-08-06 江南大学 一种高效表达碱性果胶酶的菌株及其构建与应用
CA3064037A1 (en) 2017-06-20 2018-12-27 Albumedix Ltd Improved protein expression strains
KR101984957B1 (ko) * 2017-08-09 2019-06-04 재단법인 지능형 바이오 시스템 설계 및 합성 연구단 효모의 단백질 접힘 기작(protein-folding machinery) 개량을 통한 진세노사이드 생산 증대
US11135276B2 (en) 2019-05-17 2021-10-05 Imam Abdulrahman Bin Faisal University Method of making PDIA2 and compositions containing PDIA2
JP6930670B2 (ja) 2019-06-28 2021-09-01 三菱瓦斯化学株式会社 樹脂組成物、樹脂シート、積層体、樹脂組成物層付き半導体ウェハ、樹脂組成物層付き半導体搭載用基板、及び半導体装置
US20240392279A1 (en) * 2021-09-17 2024-11-28 The University Of North Carolina At Chapel Hill Modified protein disulfide isomerase and uses thereof
GB202301988D0 (en) * 2023-02-13 2023-03-29 Phenotypeca Ltd Breeding process

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6361964B1 (en) * 1997-08-12 2002-03-26 Massachusetts Institute Of Technology Eukaryotic disulfide bond-forming proteins and related molecules and methods
US20060046253A1 (en) * 2004-09-02 2006-03-02 Suntory Limited Method for analyzing genes of industrial yeasts
US20090123452A1 (en) * 2006-07-05 2009-05-14 Madison Edwin L Protease screening methods and proteases identified thereby
US20110014651A1 (en) * 2007-10-31 2011-01-20 Kyowa Hakko Kirin Co., Ltd. Method for high-level secretory production of protein
RU2427645C1 (ru) * 2010-06-10 2011-08-27 Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП "ГосНИИгенетика") СПОСОБ МИКРОБИОЛОГИЧЕСКОГО СИНТЕЗА ЗРЕЛОГО ИНТЕРФЕРОНА АЛЬФА-2 ЧЕЛОВЕКА, ШТАММ Saccharomyces cerevisiae - ПРОДУЦЕНТ ЗРЕЛОГО ИНТЕРФЕРОНА АЛЬФА-2 ЧЕЛОВЕКА (ВАРИАНТЫ)
US8034607B2 (en) * 2007-09-12 2011-10-11 Wisconsin Alumni Research Foundation Methods of enhanced heterologous protein secretion
US8202837B2 (en) * 2005-11-04 2012-06-19 Glaxosmithkline Llc Methods for administering hypoglycemic agents

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5077204A (en) 1984-06-21 1991-12-31 Chiron Corporation Yeast endopeptidase for basic amino-acid site cleavage, preparation and use
JP2643968B2 (ja) 1988-02-03 1997-08-25 サントリー株式会社 Ｋｅｘ２エンドプロテアーゼ及びその製造方法
FR2645174B1 (fr) 1989-03-31 1994-01-07 Transgene Sa Souches de levure ameliorees pour la production de proteines heterologues matures en particulier d'hirudine et procede de preparation d'hirudine correspondant
US5545618A (en) 1990-01-24 1996-08-13 Buckley; Douglas I. GLP-1 analogs useful for diabetes treatment
GB9015825D0 (en) 1990-07-18 1990-09-05 Ciba Geigy Ag In vitro processing of fusion proteins
DK0548012T3 (da) 1991-12-16 1998-04-14 Ciba Geigy Ag Endoplasmatisk reticulum-placeret rekombinant dibasisk endoprotease samt dens anvendelse
US6291205B1 (en) 1992-06-12 2001-09-18 Merck & Co., Inc. Method of increasing production of disulfide bonded recombinant proteins by saccharomyces cerevisiae
GB9404270D0 (en) 1994-03-05 1994-04-20 Delta Biotechnology Ltd Yeast strains and modified albumins
GB9411356D0 (en) * 1994-06-07 1994-07-27 Delta Biotechnology Ltd Yeast strains
CA2198968C (en) 1996-03-04 2010-02-09 Toyofumi Masuda Process for production of secretory kex2 derivatives
JP3998717B2 (ja) * 1996-07-05 2007-10-31 ノボ ノルディスク アクティーゼルスカブ ポリペプチド類の製造方法
US7504493B2 (en) 1997-01-23 2009-03-17 The John Hopkins University Characterization of the yeast transcriptome
GB9713412D0 (en) * 1997-06-26 1997-08-27 Delta Biotechnology Ltd Improved protein expression strains
IL127127A0 (en) 1998-11-18 1999-09-22 Peptor Ltd Small functional units of antibody heavy chain variable regions
GB9902000D0 (en) * 1999-01-30 1999-03-17 Delta Biotechnology Ltd Process
ES2425738T3 (es) * 2001-12-21 2013-10-17 Human Genome Sciences, Inc. Proteínas de fusión de la albúmina
EP1729795B1 (en) 2004-02-09 2016-02-03 Human Genome Sciences, Inc. Albumin fusion proteins
DE502005005689D1 (de) 2005-05-04 2008-11-27 Signalomics Gmbh Kompetitives n-Hybrid System
JP5354559B2 (ja) 2005-11-24 2013-11-27 独立行政法人産業技術総合研究所 高効率分泌シグナルペプチド及びそれらを利用したタンパク質発現系
EP2225375A1 (en) 2007-12-21 2010-09-08 Fujifilm Manufacturing Europe B.V. Improvement of the secretion yield of a protein of interest by in vivo proteolytic processing of a multimeric precursor
US20110129872A1 (en) * 2009-12-01 2011-06-02 Mogam Biotechnology Research Institute Method for a production of a recombinant protein using yeast co-expression system
CA2890766A1 (en) * 2012-11-08 2014-05-15 Novozymes Biopharma Dk A/S Albumin variants

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6361964B1 (en) * 1997-08-12 2002-03-26 Massachusetts Institute Of Technology Eukaryotic disulfide bond-forming proteins and related molecules and methods
US20060046253A1 (en) * 2004-09-02 2006-03-02 Suntory Limited Method for analyzing genes of industrial yeasts
US8202837B2 (en) * 2005-11-04 2012-06-19 Glaxosmithkline Llc Methods for administering hypoglycemic agents
US20090123452A1 (en) * 2006-07-05 2009-05-14 Madison Edwin L Protease screening methods and proteases identified thereby
US8034607B2 (en) * 2007-09-12 2011-10-11 Wisconsin Alumni Research Foundation Methods of enhanced heterologous protein secretion
US20110014651A1 (en) * 2007-10-31 2011-01-20 Kyowa Hakko Kirin Co., Ltd. Method for high-level secretory production of protein
RU2427645C1 (ru) * 2010-06-10 2011-08-27 Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП "ГосНИИгенетика") СПОСОБ МИКРОБИОЛОГИЧЕСКОГО СИНТЕЗА ЗРЕЛОГО ИНТЕРФЕРОНА АЛЬФА-2 ЧЕЛОВЕКА, ШТАММ Saccharomyces cerevisiae - ПРОДУЦЕНТ ЗРЕЛОГО ИНТЕРФЕРОНА АЛЬФА-2 ЧЕЛОВЕКА (ВАРИАНТЫ)

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
(см. SEQ ID NO: 1). *
UniProtKB - P13134 (KEX2_YEAST), 01.01.1990. Найдено в интернет 19.01.2018 по адресу: www.uniprot.org/uniprot/P13134. UniProtKB - P17967 (PDI_YEAST), 01.03.1992. Найдено в интернет 19.01.2018 по адресу: www.uniprot.org/uniprot/P17967. UniProtKB - Q03103 (ERO1_YEAST), 01.11.1997. Найдено в интернет 19.01.2018 по адресу: www.uniprot.org/uniprot/Q03103. *
UniProtKB - P13134 (KEX2_YEAST), 01.01.1990. Найдено в интернет 19.01.2018 по адресу: www.uniprot.org/uniprot/P13134. UniProtKB - P17967 (PDI_YEAST), 01.03.1992. Найдено в интернет 19.01.2018 по адресу: www.uniprot.org/uniprot/P17967. UniProtKB - Q03103 (ERO1_YEAST), 01.11.1997. Найдено в интернет 19.01.2018 по адресу: www.uniprot.org/uniprot/Q03103. US 8202837 B2, 19.06.2012 *

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JP6742729B2 (ja) 2020-08-19
AU2014225678A1 (en) 2015-09-17
US9926570B2 (en) 2018-03-27
JP2016514957A (ja) 2016-05-26
CA2903739A1 (en) 2014-09-12
KR20150126839A (ko) 2015-11-13
JP2019122395A (ja) 2019-07-25
ES2843679T3 (es) 2021-07-20
EP2964666A1 (en) 2016-01-13
US20160017343A1 (en) 2016-01-21
SG11201504771RA (en) 2015-09-29
RU2015135980A (ru) 2017-04-10
AU2014225678B2 (en) 2016-11-17
EP2964666B1 (en) 2020-11-18
BR112015021960A2 (pt) 2017-08-29
WO2014138371A1 (en) 2014-09-12
EP2964666A4 (en) 2016-08-10

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