RU2644672C2 - Способы и наборы для обнаружения бесклеточных специфических для патогенов нуклеиновых кислот - Google Patents
Способы и наборы для обнаружения бесклеточных специфических для патогенов нуклеиновых кислот Download PDFInfo
- Publication number
- RU2644672C2 RU2644672C2 RU2013148806A RU2013148806A RU2644672C2 RU 2644672 C2 RU2644672 C2 RU 2644672C2 RU 2013148806 A RU2013148806 A RU 2013148806A RU 2013148806 A RU2013148806 A RU 2013148806A RU 2644672 C2 RU2644672 C2 RU 2644672C2
- Authority
- RU
- Russia
- Prior art keywords
- nucleic acid
- target nucleic
- cell
- double
- subject
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 94
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 76
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 76
- 238000000034 method Methods 0.000 title claims abstract description 43
- 238000001514 detection method Methods 0.000 title abstract description 26
- 244000052769 pathogen Species 0.000 title description 22
- 230000001717 pathogenic effect Effects 0.000 title description 20
- 108020004414 DNA Proteins 0.000 claims abstract description 62
- 210000004369 blood Anatomy 0.000 claims abstract description 29
- 239000008280 blood Substances 0.000 claims abstract description 29
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 22
- 241000187479 Mycobacterium tuberculosis Species 0.000 claims abstract description 17
- 230000003321 amplification Effects 0.000 claims abstract description 17
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 17
- 210000000416 exudates and transudate Anatomy 0.000 claims abstract description 10
- 239000012634 fragment Substances 0.000 claims abstract description 10
- 201000008827 tuberculosis Diseases 0.000 claims abstract description 7
- 101100381862 Bacillus subtilis (strain 168) bmr3 gene Proteins 0.000 claims abstract description 6
- 101100038261 Methanococcus vannielii (strain ATCC 35089 / DSM 1224 / JCM 13029 / OCM 148 / SB) rpo2C gene Proteins 0.000 claims abstract description 6
- 101100509674 Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) katG3 gene Proteins 0.000 claims abstract description 6
- 101100344811 Starmerella bombicola mdr gene Proteins 0.000 claims abstract description 6
- 101150092878 iniB gene Proteins 0.000 claims abstract description 6
- 101150013110 katG gene Proteins 0.000 claims abstract description 6
- 101150085857 rpo2 gene Proteins 0.000 claims abstract description 6
- 101150090202 rpoB gene Proteins 0.000 claims abstract description 6
- 239000000523 sample Substances 0.000 claims description 32
- 102000053602 DNA Human genes 0.000 claims description 26
- 210000002381 plasma Anatomy 0.000 claims description 23
- 238000003752 polymerase chain reaction Methods 0.000 claims description 20
- 208000015181 infectious disease Diseases 0.000 claims description 17
- 210000001175 cerebrospinal fluid Anatomy 0.000 claims description 15
- 239000007850 fluorescent dye Substances 0.000 claims description 14
- 238000009830 intercalation Methods 0.000 claims description 14
- 239000003795 chemical substances by application Substances 0.000 claims description 9
- 210000002966 serum Anatomy 0.000 claims description 9
- 239000007788 liquid Substances 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 21
- 239000012472 biological sample Substances 0.000 description 16
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 13
- 239000000463 material Substances 0.000 description 11
- 238000002844 melting Methods 0.000 description 11
- 230000008018 melting Effects 0.000 description 11
- 239000003153 chemical reaction reagent Substances 0.000 description 10
- 239000002773 nucleotide Substances 0.000 description 10
- 125000003729 nucleotide group Chemical group 0.000 description 10
- 239000000047 product Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 7
- 241000282414 Homo sapiens Species 0.000 description 6
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 6
- 238000010240 RT-PCR analysis Methods 0.000 description 6
- 244000045947 parasite Species 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 210000004910 pleural fluid Anatomy 0.000 description 5
- 102000040430 polynucleotide Human genes 0.000 description 5
- 108091033319 polynucleotide Proteins 0.000 description 5
- 239000002157 polynucleotide Substances 0.000 description 5
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 241000233866 Fungi Species 0.000 description 4
- 108010093628 Heterometrus granulomanus lectin Proteins 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 238000003745 diagnosis Methods 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 238000007834 ligase chain reaction Methods 0.000 description 4
- 210000005259 peripheral blood Anatomy 0.000 description 4
- 239000011886 peripheral blood Substances 0.000 description 4
- 241000282693 Cercopithecidae Species 0.000 description 3
- 206010036790 Productive cough Diseases 0.000 description 3
- 238000003759 clinical diagnosis Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000003753 real-time PCR Methods 0.000 description 3
- 239000013049 sediment Substances 0.000 description 3
- 210000003802 sputum Anatomy 0.000 description 3
- 208000024794 sputum Diseases 0.000 description 3
- 229960001005 tuberculin Drugs 0.000 description 3
- 108091093088 Amplicon Proteins 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 2
- 241000223935 Cryptosporidium Species 0.000 description 2
- 241000283073 Equus caballus Species 0.000 description 2
- 241000282326 Felis catus Species 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 2
- 108010074328 Interferon-gamma Proteins 0.000 description 2
- 208000032420 Latent Infection Diseases 0.000 description 2
- 241000222722 Leishmania <genus> Species 0.000 description 2
- 241000282560 Macaca mulatta Species 0.000 description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 2
- 241001646725 Mycobacterium tuberculosis H37Rv Species 0.000 description 2
- 241000588653 Neisseria Species 0.000 description 2
- 241000009328 Perro Species 0.000 description 2
- 241000224016 Plasmodium Species 0.000 description 2
- 241000242678 Schistosoma Species 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 208000036981 active tuberculosis Diseases 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000003146 anticoagulant agent Substances 0.000 description 2
- 229940127219 anticoagulant drug Drugs 0.000 description 2
- 238000001574 biopsy Methods 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 239000000539 dimer Substances 0.000 description 2
- 208000037771 disease arising from reactivation of latent virus Diseases 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 238000005199 ultracentrifugation Methods 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- 206010003445 Ascites Diseases 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241001037822 Bacillus bacterium Species 0.000 description 1
- 241000589562 Brucella Species 0.000 description 1
- 241000193403 Clostridium Species 0.000 description 1
- 241000193155 Clostridium botulinum Species 0.000 description 1
- 241000186581 Clostridium novyi Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 241000224431 Entamoeba Species 0.000 description 1
- 241000224432 Entamoeba histolytica Species 0.000 description 1
- 241000242711 Fasciola hepatica Species 0.000 description 1
- 241000224466 Giardia Species 0.000 description 1
- 241000224467 Giardia intestinalis Species 0.000 description 1
- 241000589989 Helicobacter Species 0.000 description 1
- 241000590002 Helicobacter pylori Species 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 102100037850 Interferon gamma Human genes 0.000 description 1
- 102000008070 Interferon-gamma Human genes 0.000 description 1
- 241000589248 Legionella Species 0.000 description 1
- 241000589242 Legionella pneumophila Species 0.000 description 1
- 208000007764 Legionnaires' Disease Diseases 0.000 description 1
- 241000186781 Listeria Species 0.000 description 1
- 241000186779 Listeria monocytogenes Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 201000009906 Meningitis Diseases 0.000 description 1
- 241000186359 Mycobacterium Species 0.000 description 1
- 241000186362 Mycobacterium leprae Species 0.000 description 1
- 108700035964 Mycobacterium tuberculosis HsaD Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 208000005228 Pericardial Effusion Diseases 0.000 description 1
- 238000011529 RT qPCR Methods 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 241000193998 Streptococcus pneumoniae Species 0.000 description 1
- 241000223996 Toxoplasma Species 0.000 description 1
- 241000223997 Toxoplasma gondii Species 0.000 description 1
- 241000242541 Trematoda Species 0.000 description 1
- 241000589886 Treponema Species 0.000 description 1
- 241000589884 Treponema pallidum Species 0.000 description 1
- 241000224526 Trichomonas Species 0.000 description 1
- 241000224527 Trichomonas vaginalis Species 0.000 description 1
- 241000223104 Trypanosoma Species 0.000 description 1
- 241000223109 Trypanosoma cruzi Species 0.000 description 1
- 101000832077 Xenopus laevis Dapper 1-A Proteins 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 210000003567 ascitic fluid Anatomy 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 238000009534 blood test Methods 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 210000000038 chest Anatomy 0.000 description 1
- YTRQFSDWAXHJCC-UHFFFAOYSA-N chloroform;phenol Chemical compound ClC(Cl)Cl.OC1=CC=CC=C1 YTRQFSDWAXHJCC-UHFFFAOYSA-N 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 238000013399 early diagnosis Methods 0.000 description 1
- 229940007078 entamoeba histolytica Drugs 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 229940044627 gamma-interferon Drugs 0.000 description 1
- 210000003736 gastrointestinal content Anatomy 0.000 description 1
- 229940037467 helicobacter pylori Drugs 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000011998 interferon-gamma release assay Methods 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 229940115932 legionella pneumophila Drugs 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 229940115931 listeria monocytogenes Drugs 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 210000004912 pericardial fluid Anatomy 0.000 description 1
- 210000004976 peripheral blood cell Anatomy 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 210000004180 plasmocyte Anatomy 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 208000008128 pulmonary tuberculosis Diseases 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 229940031000 streptococcus pneumoniae Drugs 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 235000019722 synbiotics Nutrition 0.000 description 1
- 210000001179 synovial fluid Anatomy 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161470774P | 2011-04-01 | 2011-04-01 | |
| US61/470,774 | 2011-04-01 | ||
| PCT/US2012/031814 WO2012135815A2 (en) | 2011-04-01 | 2012-04-02 | Methods and kits for detecting cell-free pathogen-specific nucleic acids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2013148806A RU2013148806A (ru) | 2015-05-10 |
| RU2644672C2 true RU2644672C2 (ru) | 2018-02-13 |
Family
ID=46932429
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2013148806A RU2644672C2 (ru) | 2011-04-01 | 2012-04-02 | Способы и наборы для обнаружения бесклеточных специфических для патогенов нуклеиновых кислот |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US20140147851A1 (cg-RX-API-DMAC7.html) |
| EP (1) | EP2694680A4 (cg-RX-API-DMAC7.html) |
| JP (2) | JP6430826B2 (cg-RX-API-DMAC7.html) |
| CN (2) | CN103814139B (cg-RX-API-DMAC7.html) |
| AU (3) | AU2012236109A1 (cg-RX-API-DMAC7.html) |
| RU (1) | RU2644672C2 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2012135815A2 (cg-RX-API-DMAC7.html) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103814139B (zh) * | 2011-04-01 | 2018-11-13 | 澳康姆生物实验室公司 | 用于检测无细胞的病原体特异性核酸的方法和试剂盒 |
| EP3923347B1 (en) * | 2011-09-30 | 2024-04-03 | Sony Group Corporation | Tungsten gates for non-planar transistors |
| TR201904004T4 (tr) | 2013-05-24 | 2019-04-22 | Occam Biolabs Inc | Bir nükleik asit numunesi toplamak için sistem ve yöntem. |
| US9644232B2 (en) | 2013-07-26 | 2017-05-09 | General Electric Company | Method and device for collection and amplification of circulating nucleic acids |
| MX383494B (es) | 2013-11-07 | 2025-03-14 | Univ Leland Stanford Junior | Ácidos nucleicos libres de células para el análisis del microbioma humano y componentes del mismo. |
| CN104894226A (zh) * | 2014-03-07 | 2015-09-09 | 复旦大学 | 检测实验室结核分枝杆菌微环境表面污染的方法及其检测试剂盒 |
| JP6735276B2 (ja) | 2014-11-21 | 2020-08-05 | オッカム バイオラブス，インコーポレイティド | 核酸サンプルを収集するためのシステム及び方法 |
| WO2016187234A1 (en) | 2015-05-18 | 2016-11-24 | Karius, Inc. | Compositions and methods for enriching populations of nucleic acids |
| EP3347448B1 (en) | 2015-09-09 | 2020-07-08 | Drawbridge Health, Inc. | Methods for sample collection, stabilization and preservation |
| EP3141612A1 (en) | 2015-09-10 | 2017-03-15 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Method and device for nucleic acid based diagnostic approaches including the determination of a deviant condtion, especially a health condition and/or pathogenic condition of a sample |
| WO2017077999A1 (ja) * | 2015-11-06 | 2017-05-11 | 公立大学法人横浜市立大学 | 結核菌群由来ｄｎａの検出方法 |
| CN105296661A (zh) * | 2015-12-02 | 2016-02-03 | 北京市结核病胸部肿瘤研究所 | 通过检测游离核酸诊断结核病的试剂盒及其应用 |
| EP3433382B1 (en) | 2016-03-25 | 2021-09-01 | Karius, Inc. | Synthetic nucleic acid spike-ins |
| JP6812431B2 (ja) * | 2016-07-05 | 2021-01-13 | 株式会社日立製作所 | Ｄｎａ検出方法およびそのための装置 |
| WO2018089860A1 (en) | 2016-11-11 | 2018-05-17 | 2D Genomics Inc. | Methods for processing nucleic acid samples |
| CA3052096A1 (en) * | 2017-01-30 | 2018-08-02 | Safeguard Biosystems Holdings Ltd. | Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms |
| US10697008B2 (en) | 2017-04-12 | 2020-06-30 | Karius, Inc. | Sample preparation methods, systems and compositions |
| CN108165560B (zh) * | 2017-12-01 | 2021-06-08 | 北京蛋白质组研究中心 | 结核分枝杆菌H37Rv编码基因及其应用 |
| CN108165563B (zh) * | 2017-12-01 | 2021-02-19 | 北京蛋白质组研究中心 | 结核分枝杆菌H37Rv编码基因及其应用 |
| CN108165562B (zh) * | 2017-12-01 | 2021-06-08 | 北京蛋白质组研究中心 | 结核分枝杆菌H37Rv编码基因及其应用 |
| CN108165561B (zh) * | 2017-12-01 | 2021-06-18 | 北京蛋白质组研究中心 | 结核分枝杆菌H37Rv编码基因及其应用 |
| WO2019178157A1 (en) | 2018-03-16 | 2019-09-19 | Karius, Inc. | Sample series to differentiate target nucleic acids from contaminant nucleic acids |
| EP3884065B1 (en) | 2018-11-21 | 2024-08-14 | Karius, Inc. | Direct-to-library methods, systems, and compositions |
| CN111876411A (zh) * | 2019-09-06 | 2020-11-03 | 深圳微伴生物有限公司 | 获得cfDNA标准品的引物组、PCR扩增阳性标准品及其制备方法、试剂盒及应用 |
| KR102286570B1 (ko) * | 2019-11-19 | 2021-08-05 | 대한민국 | 소변 시료를 이용하여 결핵 진단용 프라이머, 프로브, 키트 및 이들의 용도 |
| CN114480691A (zh) * | 2022-01-24 | 2022-05-13 | 广州迪澳基因科技有限公司 | 基于熔解曲线检测结核分枝杆菌复合菌群的方法及试剂盒 |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11225797A (ja) * | 1997-11-28 | 1999-08-24 | Centeon Pharma Gmbh | 血漿および／または血清中の高ウイルス濃度をポリメラーゼ連鎖反応を用いて検出する方法 |
| RU98116294A (ru) * | 1996-01-26 | 2000-06-27 | Вирко Н.В. | Способ определения оптимальной химиотерапии пациентов, серопозитивных по вич, основанный на фенотипической лекарственной чувствительности человеческих штаммов вич |
| US7270982B2 (en) * | 1999-12-14 | 2007-09-18 | Joy Biomedical Corporation | Helicobacter pylori antigens in blood |
| EA014648B1 (ru) * | 2005-11-15 | 2010-12-30 | Генойд Кфт. | Способ обнаружения патогенов |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0528306B1 (en) * | 1991-08-15 | 1999-11-17 | F. Hoffmann-La Roche Ag | Mycobacterium primer pair |
| US6458366B1 (en) * | 1995-09-01 | 2002-10-01 | Corixa Corporation | Compounds and methods for diagnosis of tuberculosis |
| WO1998050583A1 (en) * | 1997-05-02 | 1998-11-12 | Gen-Probe Incorporated | Two-step hybridization and capture of a polynucleotide |
| US6951722B2 (en) * | 1999-03-19 | 2005-10-04 | Takara Bio Inc. | Method for amplifying nucleic acid sequence |
| EP1315814A2 (en) * | 2000-07-24 | 2003-06-04 | Inpharmatica Limited | Adhesion molecules |
| CN1388378A (zh) * | 2002-06-17 | 2003-01-01 | 四川大学 | 结核分枝杆菌诊断检测试剂 |
| WO2005075683A1 (en) * | 2004-02-03 | 2005-08-18 | Postech Foundation | High throughput device for performing continuous-flow reactions |
| CA2609636A1 (en) * | 2005-02-17 | 2006-08-24 | Istituto Nazionale Per Le Malattie Infettive Irccs Lazzaro Spallanzani | Compositions and methods for detecting pathogen specific nucleic acids in urine |
| US8741565B2 (en) * | 2005-12-30 | 2014-06-03 | Honeywell International Inc. | Oligonucleotide microarray for identification of pathogens |
| US20090142757A1 (en) * | 2007-11-30 | 2009-06-04 | Asiagen Corporation | Strip and method for detecting nucleotide amplification products of mycobacterium tuberculosis and non-tuberculous mycobacterium |
| US8029806B2 (en) * | 2008-04-16 | 2011-10-04 | Asiagen Corporation | Method for diagnosing of Mycobacterium tuberculosis |
| US8906621B2 (en) * | 2009-01-06 | 2014-12-09 | Qimin You | Cross priming amplification of target nucleic acids |
| CN103814139B (zh) * | 2011-04-01 | 2018-11-13 | 澳康姆生物实验室公司 | 用于检测无细胞的病原体特异性核酸的方法和试剂盒 |
-
2012
- 2012-04-02 CN CN201280026842.3A patent/CN103814139B/zh not_active Expired - Fee Related
- 2012-04-02 JP JP2014502902A patent/JP6430826B2/ja not_active Expired - Fee Related
- 2012-04-02 AU AU2012236109A patent/AU2012236109A1/en not_active Abandoned
- 2012-04-02 WO PCT/US2012/031814 patent/WO2012135815A2/en not_active Ceased
- 2012-04-02 CN CN201811203829.6A patent/CN109280713A/zh active Pending
- 2012-04-02 RU RU2013148806A patent/RU2644672C2/ru not_active IP Right Cessation
- 2012-04-02 EP EP12765867.2A patent/EP2694680A4/en not_active Withdrawn
- 2012-04-02 US US14/009,199 patent/US20140147851A1/en not_active Abandoned
-
2017
- 2017-05-09 AU AU2017203071A patent/AU2017203071A1/en not_active Abandoned
- 2017-07-31 US US15/664,074 patent/US20170369932A1/en not_active Abandoned
-
2018
- 2018-05-10 JP JP2018091537A patent/JP2018117645A/ja active Pending
-
2019
- 2019-07-05 AU AU2019204844A patent/AU2019204844A1/en not_active Abandoned
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU98116294A (ru) * | 1996-01-26 | 2000-06-27 | Вирко Н.В. | Способ определения оптимальной химиотерапии пациентов, серопозитивных по вич, основанный на фенотипической лекарственной чувствительности человеческих штаммов вич |
| JPH11225797A (ja) * | 1997-11-28 | 1999-08-24 | Centeon Pharma Gmbh | 血漿および／または血清中の高ウイルス濃度をポリメラーゼ連鎖反応を用いて検出する方法 |
| US6183999B1 (en) * | 1997-11-28 | 2001-02-06 | Aventis Behring Gmbh | Procedure for the detection of high virus concentrations in blood plasma and/or blood serum by means of the polymerase chain reaction |
| US7270982B2 (en) * | 1999-12-14 | 2007-09-18 | Joy Biomedical Corporation | Helicobacter pylori antigens in blood |
| EA014648B1 (ru) * | 2005-11-15 | 2010-12-30 | Генойд Кфт. | Способ обнаружения патогенов |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103814139B (zh) | 2018-11-13 |
| RU2013148806A (ru) | 2015-05-10 |
| AU2012236109A1 (en) | 2013-11-21 |
| EP2694680A4 (en) | 2014-09-10 |
| CN109280713A (zh) | 2019-01-29 |
| US20170369932A1 (en) | 2017-12-28 |
| JP2014510538A (ja) | 2014-05-01 |
| WO2012135815A3 (en) | 2012-12-27 |
| AU2017203071A1 (en) | 2017-06-01 |
| JP2018117645A (ja) | 2018-08-02 |
| AU2019204844A1 (en) | 2019-07-25 |
| JP6430826B2 (ja) | 2018-11-28 |
| WO2012135815A2 (en) | 2012-10-04 |
| US20140147851A1 (en) | 2014-05-29 |
| CN103814139A (zh) | 2014-05-21 |
| EP2694680A2 (en) | 2014-02-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2644672C2 (ru) | Способы и наборы для обнаружения бесклеточных специфических для патогенов нуклеиновых кислот | |
| McKenna et al. | Evaluation of three ELISAs for Mycobacterium avium subsp. paratuberculosis using tissue and fecal culture as comparison standards | |
| Deshpande et al. | Evaluation of the IS 6110 PCR assay for the rapid diagnosis of tuberculous meningitis | |
| Keid et al. | Diagnosis of canine brucellosis: comparison between serological and microbiological tests and a PCR based on primers to 16S-23S rDNA interspacer | |
| Modi et al. | Multitargeted loop-mediated isothermal amplification for rapid diagnosis of tuberculous meningitis | |
| Castilho et al. | A real-time polymerase chain reaction assay for the identification and quantification of American Leishmania species on the basis of glucose-6-phosphate dehydrogenase | |
| Kuk et al. | Stool sample storage conditions for the preservation of Giardia intestinalis DNA | |
| US5942394A (en) | Detection of protozoan parasites | |
| De las Heras et al. | A PCR technique for the detection of Jaagsiekte sheep retrovirus in the blood suitable for the screening of ovine pulmonary adenocarcinoma in field conditions | |
| US20150152485A1 (en) | Mycobacterium tuberculosis detection using transrenal dna | |
| WO2023279042A2 (en) | Compositions and methods for detection of severe acute respiratory syndrome coronavirus 2 variants | |
| WO2022076428A1 (en) | Methods of diagnosing tuberculosis and differentiating between active and latent tuberculosis | |
| El Kholy et al. | Diagnosis of human brucellosis in Egypt by polymerase chain reaction. | |
| JP2012531908A (ja) | 結核菌を検出するための方法および／またはプライマー | |
| Che-Engku-Chik et al. | Detection of tuberculosis (TB) using gold standard method, direct sputum smears microscopy, PCR, qPCR and electrochemical DNA sensor: A mini review | |
| KR101395938B1 (ko) | 장수풍뎅이 질병 유발 세균 진단용 프라이머 및 그 진단방법 | |
| Chiabchalard et al. | Sensitivity and specificity of polymerase chain reaction for the detection of Toxoplasma gondii DNA added to laboratory samples | |
| HK40003844A (en) | Methods and kits for detecting cell-free pathogen-specific nucleic acids | |
| WO2021087174A1 (en) | Method and kit for direct detection of bacterial blood diseases | |
| Khudhur et al. | Increasing of Macrophage Migration Inhibitory Factor Expression in Human Patients Infected with Virulent Brucella in Iraq | |
| CN111154898A (zh) | 鉴定人结核分枝杆菌、牛分枝杆菌和卡介苗的技术方法 | |
| RU2795987C1 (ru) | Набор олигонуклеотидных праймеров и флуоресцентно-меченых зондов и способ выявления ДНК возбудителя бруцеллеза | |
| KR102526656B1 (ko) | 핵산 검출 방법 | |
| Saud et al. | Molecular detection of Brucella canis in Blood of Dogs. | |
| JP4831467B2 (ja) | ヨーネ病診断用プライマーおよびウロコルチン遺伝子の発現量測定によるヨーネ病の診断法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20190403 |