RU2618103C1 - Method for producing functional forage - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: method involves soaking the seeds in electro-activated water, germinating and seedlings forcing. The seeds are represented by lupine seeds. Washing the lupine seeds is carried out with tap water for 4-8 minutes, thereinafter the washed seeds are soaked in anolyte with the pH of 2.4-8.0 units and the redox potential of 230-810 mV, the oxygen concentration of 6.0-14.8 mg/l, obtained by the contact activation of 6-10% ammonium sulfate solution, for 3.5-4.5 hours at the ratio of seeds to anolyte 1:2. Thereafter, the anolyte is removed, and the re-washing of seeds is carried out with tap water for 3-8 minutes. Germinating the seeds is carried out in a thin layer without the use of the substrate by the air-irrigation method at periodic tedding, with the total germination duration of 6-8 days under natural light.

EFFECT: using the invention will allow to obtain high-quality forage by accelerating the production process of seeds germination and to reduce its duration, as well as to obtain functional forage for livestock and poultry, with recommended biochemical and microbiological quality indices.

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Description

The invention relates to agriculture, and in particular to methods for the production of functional feed for feeding farm animals and birds.

A known method of feed vitamin supplements, characterized by grinding the feedstock, adding water, heat treating the obtained substrate, fermenting the cultivation of yeast under aeration on a nutrient medium, followed by isolation of the target product, obtain a suspension with a dry matter concentration of at least 6% and for more than 24 hours from the time of preparation, they are preserved with hypochlorite in the form of 5% aqueous solutions in a dosage of 0.005-0.01% to the volume of the suspension (according to active chlorine) (US Pat. RU No. 2290831).

The disadvantages of the method of obtaining feed vitamin supplements are significant energy costs, as well as an additional intake - the cultivation of yeast cultures, as well as the use of preservative agents, which, in turn, can have an adverse effect on the body of farm animals.

There is also known a method of growing green hydroponic feed (US Pat. RU No. 2429592), including pre-soaking the seed with activated water with a pH of 8-10 obtained by electrolysis in a rarefied medium at a pressure of 650-680 mm Hg with simultaneous stirring for 5-9 minutes with a drum speed of 10 rpm

The disadvantage of this method is the use for soaking seed of a vacuum medium created by a special device.

The closest prototype analogue to the claimed one is a known method of producing hydroponic green fodder, which involves soaking seeds in catholyte of electroactivated water, which is obtained by electroactivation of a 4-6% solution of ammonium sulfate with a pH of 9-10, redox potential (-800) - ( -900) mV, with a specific consumption of electricity of 0.062-0.070 A / h per 1 liter of catholyte and anolyte, soaked for 3-5 hours with a total germination time of 10 days: the first 2 days without lighting, the next 8 days when lighting development, and forcing seedlings (US Pat. RU No. 2524538 - prototype).

The disadvantages of this method are the significant complexity of the process of germination of grain and its complication due to the use of two phases of germination - without light and in its presence, as well as the formation of ammonia on the cathode during electrolysis, which can have an adverse effect on the body of farm animals and birds. This method does not allow to reduce the duration of germination without loss of feed quality.

Known methods do not allow to obtain high-quality functional food in a short time without additional labor and loss of quality of raw materials.

The technical result is to increase the energy of lupine, increase the yield of biomass and plant productivity (feed), to protect the seedlings from diseases and accelerate the technological process of production of functional feed.

The technical result is achieved by the fact that in a method for the production of functional food, including soaking seeds in electroactivated water, germinating and sprouting sprouts, according to the invention, the lupine seeds are washed with tap water for 4-8 minutes, after which the washed seeds are soaked with anolyte with a pH of 2.4 -8.0 units and a redox potential of 230-810 mV, an oxygen concentration of 6.0-14.8 mg / l, obtained by contact activation of a 6-10% solution of ammonium sulfate for 3.5-4.5 hours, with a seed ratio to the anolyte 1: 2, then the anolyte is removed and the seeds are re-flushed with tap water for 3-8 minutes, and the seeds are germinated in a thin layer without using the substrate by the air-irrigation method with periodic tedding, with a total germination time of 6-8 days in natural light.

The novelty of the proposed method for the production of functional food consists in increasing the energy of germination of lupine seeds and increasing the yield of biomass and plant productivity (feed) by protecting the seedlings from diseases, as a result of the use of anolyte with a pH of 2.4-8.0 units. and a redox potential of 230-810 mV, an oxygen concentration of 6.0-14.8 mg / l, as well as a reduction in germination time without loss of feed quality with minimal material and labor costs.

The features that distinguish the claimed technical solution from the prototype are aimed at achieving a technical result and were not identified when studying patent and scientific and technical literature in this and related fields of science and technology and, therefore, meet the criterion of "inventive step".

The proposed method for the production of functional feed, it is possible to apply in industrial specialized enterprises.

Anolyte solution for the treatment of lupine seeds was obtained by contact activation of a 6-10% solution of ammonium sulfate at a current strength of 5 A for 12-15 minutes. This time and amperage is sufficient to obtain anolyte with specified physicochemical parameters. The current strength for obtaining a high-quality anolyte should be 5 A. If the current strength during processing is less than 5 A, then it will not be enough to acidify the anolyte to the required value and the hydrolysis process will slow down. If the current strength for processing is more than 5 A, then due to the increase in resistance, part of the energy consumption will be spent on heating the solution, which will increase the current consumption and reduce the rate of increase in pH.

If the time of washing the seeds from dust and impurities is less than 4 minutes, then this time will not be enough for high-quality washing of seeds, if the washing time is more than 8 minutes, this will slow down the germination technology, so the optimal time for washing seeds from impurities and dust is 6 minutes.

If the claimed method for the production of functional feed during the soaking of seeds, the pH of the anolyte is less than 2.4 units, this contributes to the inhibitory effect of the processes of seed germination in view of the increased acidity of the anolyte medium, which leads to a decrease in the germination energy of lupine seeds and a decrease in the biomass yield and plant productivity (stern). If the pH is greater than 8.0 units, this leads to a slowdown in moisture consumption by the seed coat and increases the germination time of the feed additive, so 5.2 units are optimal for the anolyte used as a lock solution for seeds.

If the anolyte ORP is less than 230 mV, then the stimulating effect of the anolyte on the biochemical processes of seeds decreases, and the germination energy decreases, and if the ORP is more than 810 mV, this leads to an increase in oxidative processes in solution, which slows down the activity of the enzyme systems of the seeds, the biomass yield plants; therefore, an ORP of 520 mV is optimal for anolyte.

If the oxygen concentration in the anolyte is less than 6.0 mg / l, then this significantly reduces the process of saturation with moisture by the seed coat and, as a result, reduces the activity of seed swelling, which slows down the germination of seeds and increases the time of germination. If the oxygen concentration in the anolyte is more than 14.8 mg / l, then a high degree of oxygen saturation of the solution increases oxidative reactions, which slows down the functioning of the enzyme systems of the seeds and worsens the germination energy, therefore, the optimal oxygen concentration is 10.4 mg / l.

If the ratio of seeds to anolyte is greater, this contributes to the development of unfavorable microflora, which makes it difficult to germinate, if less, saturation of the endosperm of the seed with moisture will be insufficient, which will also slow down the process of germination. Therefore, the optimal ratio of seeds to anolyte, respectively, is 1: 2.

The optimal time for repeated flushing of seeds after soaking in anolyte is 5 minutes. Rinsing less than 5 minutes is not enough to remove anolyte residues from the seeds, if more - this helps to increase the time of the process of germination of feed.

The claimed optimal ratio of seeds to anolyte, equal to a ratio of 1: 2. If the ratio of seeds to anolyte is greater, this contributes to the development of unfavorable microflora, which makes it difficult to germinate, if less, saturation of the endosperm of seeds with moisture will be insufficient, which will also slow down the process of germination.

Method for the production of functional feed is as follows.

Rinsing of lupine seeds is carried out with tap water for 4-8 minutes. After which the washed seeds are soaked with anolyte with a pH of 2.4-8.0 and a redox potential of 230-810 mV, an oxygen concentration of 6.0-14.8 mg / l for 3.5-4.5 hours, with a ratio of seeds to anolyte 1: 2. After that, the anolyte is removed and the seeds are re-flushed with tap water for 3-8 minutes. Germination of seeds is carried out in a thin layer without using a substrate by the air-irrigation method with periodic tedding, with a total duration of germination of 6-8 days in natural light.

An example of the method

1 kg of Start lupine is taken and washed for 6 min under tap water, the weed impurity, dust are removed from the seed mass and placed in one layer in a low plastic container without a substrate, pouring with an anolyte obtained by contact by electrolysis of an 8% sulfate solution ammonium, in the ratio of seeds to anolyte 1: 2 for 4 hours at an ambient temperature of 18 ° C.

After 4 hours, the anolyte is carefully drained and the seeds are repeatedly washed with tap water to remove residual anolyte from the seed mass, after which the stage of air-irrigation germination begins. The swollen seeds are laid out in a thin layer on plastic spreads and covered with wet gauze and left at a temperature of 18 ° C and natural light, periodically irrigating the seeds with water, begin to expel green biomass.

As a result of microbiological studies when sowing seeds, processed according to the proposed method, on the nutrient medium MPA and Chapek medium, the degree of contamination of the fungal and bacterial microflora was minimal.

Table 1 presents the difference in the energy indices of germination of lupine seeds sprouted using anolyte obtained by contactless activation, with the specified parameters and the prototype methodology (control) in five replicates. The germination energy of lupine seeds was determined according to GOST 10968-88. Data biochemical studies and the content of vitamins in seedlings of lupine are presented in table 2.

On average, the difference in the energy of germination of lupine seeds between the control variant (prototype) and the experimental one (by the proposed method) was 9.8%.

The following vitamins were determined in the green mass: B1 - 2 mg / 100 g, B2 - 0.7 mg / 100 g, B3 - 4.5 mg / 100 g, B6 - 3 mg / 100 g, E - 21 mg / 100 g.

Thus, the presented biochemical data allow us to conclude that the functional feed is suitable for inclusion in the diet of farm animals and birds.

As can be seen, the treatment of lupine seeds with an anolyte obtained by a non-contact method with the stated parameters significantly reduces the seed germination time by 72 hours due to increased germination energy and plant productivity, as well as an increase in biomass yield by protecting plants from contaminating microorganisms.

The proposed method for the production of functional feed will reduce energy and labor costs in connection with a single use of anolyte without additional treatments with other solutions, the method does not require special equipment for seed germination.

Claims (1)

Method for the production of functional food, including soaking seeds in electroactivated water, germinating and pasturing seedlings, characterized in that the seeds are lupine seeds, the lupine seeds are washed with tap water for 4-8 minutes, after which the washed seeds are soaked with anolyte with a pH of 2 , 4-8.0 units and a redox potential of 230-810 mV, an oxygen concentration of 6.0-14.8 mg / l, obtained by contact activation of a 6-10% solution of ammonium sulfate, for 3.5-4.5 hours at a seed ratio to the anolyte 1: 2, then the anolyte is removed and the seeds are re-flushed with tap water for 3-8 minutes, and the seeds are germinated in a thin layer without using the substrate by the air-irrigation method with periodic tedding, with a total germination time of 6-8 days in natural light.