RU2612347C1 - Method of nanocapsules production of dorogov antiseptic-excitor (dae) fraction 2 in konjac gum - Google Patents

Abstract

FIELD: nanotechnology.

SUBSTANCE: method of obtaining of Dorogov antiseptic stimulator nanocapsules fraction 2 in konjac gum is characterized in that the Dorogov antiseptic stimulator fraction 2 is dispersed into the konjac gum suspension in benzene in the presence of 0.01 g of E472c preparation as a surfactant while stirring 1300 r/min, then 5 ml of methylene chloride are added, the precipitate is filtered off and dried at room temperature, and the core:shell ratio is 1:1 or 1:3.

EFFECT: nanocapsules manufacture process simplification and acceleration and weight yield increase.

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Description

The invention relates to the field of nanotechnology.

Previously known methods for producing microcapsules.

In US Pat. 2173140, IPC A61K 009/50, A61K 009/127, Russian Federation, published September 10, 2001, a method for producing silicon organolipid microcapsules using a rotary-cavitation unit with high shear forces and powerful sonar acoustic and ultrasonic dispersion ranges is proposed.

The disadvantage of this method is the use of special equipment - a rotary cavitation unit, which has an ultrasonic effect, which affects the formation of microcapsules and can cause adverse reactions due to the fact that ultrasound destructively affects polymers of a protein nature, therefore, the proposed method is applicable when work with polymers of synthetic origin

In US Pat. 2359662, IPC A61K 009/56, A61J 003/07, B01J 013/02, A23L 001/00, published June 27, 2009, Russian Federation, a method for producing sodium chloride microcapsules using spray cooling in a Niro spray cooling tower under the following conditions: temperature inlet air 10 ° C, outlet air temperature 28 ° C, spray drum rotation speed 10,000 rpm. The microcapsules of the invention have improved stability and provide controlled and / or prolonged release of the active ingredient.

The disadvantages of the proposed method are the duration of the process and the use of special equipment, a set of certain conditions (air temperature at the inlet 10 ° C, air temperature at the outlet 28 ° C, rotation speed of the spray drum 10,000 rpm).

The closest method is the method proposed in US Pat. 2134967, IPC A01N 53/00, A01N 25/28, published on 08.27.1999, Russian Federation (1999). A solution of a mixture of natural lipids and a pyrethroid insecticide in a weight ratio of 2-4: 1 in an organic solvent is dispersed in water, which simplifies the microencapsulation method.

The disadvantage of this method is dispersion in an aqueous medium, which makes the proposed method inapplicable for producing microcapsules of water-soluble preparations in water-soluble polymers.

The technical task is to simplify and accelerate the process of obtaining nanocapsules, reduce losses in obtaining nanocapsules (increase in yield by mass).

The solution of the technical problem is achieved by the method of producing nanocapsules of the Dorogov activator-stimulator (ASD) 2 fraction, characterized in that konjac gum is used as the shell of the nanocapsules, and the ASD 2 fraction as the core when nanocapsules are prepared by the non-solvent precipitation method using methylene chloride as a precipitant .

A distinctive feature of the proposed method is the production of nanocapsules by non-solvent precipitation using methylene chloride as a precipitant, as well as the use of konjac gum as a shell and ASD 2 fraction as a core.

The result of the proposed method is to obtain nanocapsules ASD 2 fraction in the shell of konjac gum.

ASD 2 fraction is a tissue preparation of animal origin. It contains: compounds with an active sulfhydryl group, derivatives of aliphatic amines, carboxylic acids, aliphatic and cyclic hydrocarbons, derivatives of amides and water. When administered orally, ASD-2 has an activating effect on the central nervous system and the autonomic nervous system, stimulates the motor activity of the gastrointestinal tract, secretion of digestive glands, increases the activity of digestive and tissue enzymes, improves the penetration of Na + and K + ions through cell membranes, and helps normalize digestion processes, assimilation of nutrients and increase the body's natural resistance. For external use, the drug stimulates the activity of the reticuloendothelial system, normalizes trophism and accelerates the regeneration of damaged tissues, has a pronounced antiseptic and anti-inflammatory effect.

EXAMPLE 1

Obtaining nanocapsules ASD 2 fraction in konjak gum, the ratio of core: shell 1: 3

1 g of ASD 2 fraction is dispersed in a suspension of konjac gum in benzene containing 3 g of the indicated polymer in the presence of 0.01 g of the preparation E472c (glycerol ester with one or two molecules of food fatty acids and one or two molecules of citric acid, with citric acid as tribasic can be esterified with other glycerides and as an acid with other fatty acids. Free acid groups can be neutralized with sodium) as a surfactant with stirring at 1300 rpm. Next, 5 ml of methylene chloride are poured. The precipitate formed is filtered off and dried at room temperature.

Received 4 g of nanocapsule powder. The yield was 100%.

EXAMPLE 2

Obtaining nanocapsules ASD 2 fraction in konjac gum, the ratio of core: shell 1: 1

1 g of ASD 2 fraction is dispersed in a suspension of konjac gum in benzene containing 1 g of the specified polymer in the presence of 0.01 g of the preparation E472c (glycerol ester with one or two molecules of food fatty acids and one or two molecules of citric acid, with citric acid, as tribasic, it can be esterified with other glycerides and as an acid with other fatty acids. Free acid groups can be neutralized with sodium) as a surfactant with stirring at 1300 rpm. Next, 5 ml of methylene chloride are poured. The precipitate formed is filtered off and dried at room temperature.

Received 2 g of nanocapsule powder. The yield was 100%.

EXAMPLE 3

Sizing nanocapsules by NTA method

The measurements were carried out on a Nanosight LM0 multiparameter nanoparticle analyzer manufactured by Nanosight Ltd (Great Britain) in the HS-BF configuration (Andor Luca high-sensitivity video camera, 405 nm semiconductor laser with a power of 45 mW). The device is based on the Nanoparticle Tracking Analysis (NTA) method described in ASTM E2834.

The optimal dilution for dilution was 1: 100. For the measurement, the device parameters were selected: Camera Level = 16, Detection Threshold = 10 (multi), Min Track Length: Auto, Min Expected Size: Auto. duration of a single measurement of 215s, the use of a syringe pump.

Claims (1)

A method of producing nanorodules of the Dorogov antiseptic stimulator (ASD) 2 fraction in konjac gum, characterized in that the ASD 2 fraction is dispersed in a suspension of konjac gum in benzene in the presence of 0.01 g of the preparation E472c as a surfactant with stirring at 1300 rpm 5 ml of methylene chloride are then poured, the precipitate formed is filtered off and dried at room temperature, and the core: shell ratio can be 1: 1 or 1: 3.

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