RU2610307C1 - Method for increasing work efficiency and reduction of rehabilitation period of sport horses - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention relates to veterinary, namely to method of activation of physiological processes and increase of work efficiency of sport horses of riding breeds. Method for increasing work efficiency and reduction of rehabilitation period of sport horses consists in the following: physiological probiotic preparation Vetom 3.22 is given to animals with forage in two courses before beginning of competitions and after their end. First course: 1 time per day in dose 50 mcg/kg, 5 days running, then 5 times - 1 time per 2 days. Second course: 1 time per day in dose 50 mcg/kg, 3 cycles of 3 days with 3 day interval.

EFFECT: invention makes it possible to increase content of erythrocytes and hemoglobin in blood, as well as reduce level of urea and creatinine in blood serum of horses, which contributes to work efficiency increase and reduction of rehabilitation period after loads.

4 dwg, 4 tbl

Description

The invention relates to veterinary medicine, and in particular to a method of enhancing physiological processes and improving the performance of sport horses of high breeds.

A number of methods are known for increasing the physiological status, working capacity, and improving the results of competitions in horses participating in classical equestrian sports. Among them are the use of preparations containing placenta extract (RF Patent No. 2203073 dated 04/27/03), reindeer antler extract (RF Patent No. 2406511 dated 10/29/09), Gamavit preparation (RF Patent No. 2005475 10/01/91), inhalations mixtures of oxygen and carbon dioxide in different ratios (RF Patent No. 2055470 of 03.03.93).

A preparation based on an extract from reindeer antlers is used as an intramuscular injection three times with an interval of 5 days 30 days before the competition. The result of the use of the drug is an increase in working capacity and a reduction in the recovery period after exertion.

The disadvantage of the drug is that for storage and transportation it is necessary to observe the temperature regime and the scheme for its use is quite complicated.

The use of the drug based on the placenta extract implies a single intramuscular injection of the horse 5 hours before participating in the competition at a dose of 50-70 ml.

The disadvantage of using this method is that for the introduction of a large volume of the drug it must be injected into several different places to prevent excessive stretching of muscle tissue, which is difficult when working with animals. It is also important that during the competition the persons responsible for the horse are not allowed to administer the drugs to the horses through injection needles and syringes without the special permission of the competition veterinary commission. Otherwise, a fine is imposed on the person in charge, syringes, needles and illegal drugs are confiscated and the horse is disqualified (Veterinary Regulations, 1st edition of 01/01/12. // Protocol of the FKSR Bureau No. B-0-12 / 11 of 12/14/11. - 50 p.). Thus, the use of the drug based on the placenta extract is associated with possible difficulties in its administration and does not comply with the veterinary regulations of the Equestrian Federation of Russia (hereinafter - FKSR) and the International Equestrian Federation (hereinafter - FEI).

The drug "Gamavit" is recommended to be administered to horses 20-25 ml intramuscularly 2 times a week in a full course of 8-10 injections.

The use of this scheme is associated with high costs for the preparation, the work of veterinary specialists and possible difficulties in the application of unbalanced and shy horses.

A method of increasing the performance of horses using inhalation mixtures involves carrying out gas inhalation with a low oxygen content. The application regimen includes multiple (3-10) introduction of gas mixtures for 30-40 minutes.

This method is difficult to apply to shy and unbalanced horses. To use this method of stimulating the performance of horses in competitions, the permission of the veterinary commission of the competition is required (Veterinary Regulations, 1st edition of 01/01/12. // Protocol of the FKSR Bureau No. B-0-12 / 11 of 12/14/11 - 50 p.) .

A method of increasing the working capacity and reducing the recovery period of sports horses, including the use of a non-doping drug based on Bacillus amyloliquifaciens spores, characterized in that the animals are given a physiological probiotic preparation with feed Vet 3.22 in two courses before and after the competition, moreover:

first course: 1 time per day at a dose of 50 μl / kg, 5 days in a row, then 5 applications 1 time in 2 days;

second course: 1 time per day at a dose of 50 μl / kg, 3 cycles of 3 days with an interval of 3 days. An advantage of the described invention is the use of a non-doping drug physiological for horses, according to technological schemes for maintenance and operation, using the route of administration of unregulated and not prohibited FCCR and FEI. The introduction of the drug orally has several advantages. When taking the drug with low-dose feed, the stress factor for the horse is excluded.

The studied drug and its components are not included in the list of substances prohibited at competitions. The drug is used in small doses, a short period and has a long period of aftereffect. The bacteria contained in the preparation are highly resistant to the action of digestive juice and gastric enzymes and the ability to quickly colonize the intestines. In the intestines of horses, bacterial spores are transformed into vegetative forms and secrete various biologically active substances. Under their influence, the intestinal biocenosis, acidity of the environment, digestion, absorption and metabolism of macro- and microelements, proteins, carbohydrates, amino acids are normalized in the body (Pharmacology V.D. Sokolov et al., 2010).

Biologically active drug Vetom 3.22. contains: microorganisms Bacillus amyloliquefaciens strain VKPM B-10642 (DSM 24614), corn extract fermented by these microorganisms, sodium chloride and distilled water (A.I. Lelyak, 2012). The ease of use of the liquid form of the drug in horses is due to the small amount of the drug, which is applied to a standard cube of refined sugar and fed to the animal. In case of refusal or impossibility of using sugar during feeding, the preparation can be mixed with a small amount of feed. Applying this method in practice, it was possible to minimize the loss of the drug when it is introduced into the horse's body.

Blueprints:

In FIG. Figure 1 shows the dynamics of changes in the red blood cell count in horses of the experimental and control groups during the study.

In FIG. 2 shows the dynamics of changes in the hemoglobin content in the blood of horses of the experimental and control groups during the study.

In FIG. 3 shows the dynamics of changes in the concentration of urea in the blood serum of horses from the experimental and control groups during the study.

In FIG. 4 shows the dynamics of changes in the concentration of creatinine in the blood serum of horses of the experimental and control groups during the studies.

Tests of the drug were conducted at the Novosibirsk Hippodrome. For research, 4 groups of horses were selected. The experimental and control group at the age of 6 years and the experimental and control group at the age of 12 years, 3 horses in each group. All animals took part in show jumping, dressage and eventing competitions. The horses of the experimental groups were given the drug at a dose of 50 μl / kg in 2 courses: before the start of the competition and after their completion. The animals of the control group did not use the drug.

In animals of the experimental groups, the drug was used according to the scheme: 5 days daily, then 1 time in 2 days. The cycle of the drug was 10 appointments. As a result of the use of the drug according to this scheme, bacteria are colonized in the digestive tract in the first 5 days, the following 5 applications are aimed at maintaining the necessary concentration of microorganisms in the gastrointestinal tract. As a result of the use of the 1st course of the drug, optimal conditions are created in the horse's body to achieve a peak in performance at the start of the competition.

After the end of the competition, the 2nd course of the drug was administered - 3 consecutive days after 3 days, a total of 9 applications. The use of the 2nd course of the drug according to the proposed scheme is aimed at restoring normal physiological parameters by maintaining the required number of microorganisms in the digestive tract of horses. Control studies to study the effectiveness of the drug, according to the proposed schemes, were carried out before the use of the drug and after cessation of its purpose.

It was established that the content of red blood cells in the blood of animals of the experimental groups of 6 and 12 years of age changed (table. 1). Before using the drug in horses of 6 years of age, the studied indicator was 10.04% lower than that of the analogues from the control. After the use of the drug was completed before the competition, the red blood cell count in the blood of the experimental and control groups increased by 5.36 and 3.84%, respectively, from the initial data. After participating in the competition, the number of red blood cells in the blood of horses from the experimental and control groups increased by 32.46 and 15.24%, respectively. During this period, the experimental animals exceeded the control analogues by 3.53% in terms of red blood cell counts. After completion of the 2nd course of drug administration in the period after the competition, the horses of the experimental group had a 5.81% decrease in the erythrocyte count within the physiological norm, and 12.58% in the control group. Experimental animals in this period, in terms of the content of red blood cells in blood, exceeded the data of analogues from the control by 11.56%. Thus, when Vetom 3.22 is used, there is an increase in the content of red blood cells in the blood both when using the drug before the competition and after the competition.

In horses born in 2002, similar changes in the concentration of red blood cells in the blood were observed (Table 1). After completion of the first course of the drug, the animals of the experimental group exceeded the analogues from the control by 13.64%. After completing the 2nd course of the drug administration, the experimental horses exceeded the control animals by 25.17% in terms of the red blood cell count. After participating in the competition, the performance of the experimental and control groups increased by 15.43 and 10.76%. In the recovery period, the drug had a pronounced effect on maintaining the concentration of red blood cells in the blood of horses of 12 years of age, which favorably affects the restoration of muscle function of the body. In both age groups of animals, the indicators changed with the same regularity and were within the physiological norm for adult riding horses.

Before the use of the drug, the hemoglobin content in the horses of the experimental and control groups of 2006 and 2002 of birth did not have significant differences (Table 2). After the completion of the 1st course of the drug administration, the hemoglobin content in the blood of horses from the experimental groups of 2006 and 2002 was increased by 26.15 and 14.28%, respectively. After the competition, the animals of the experimental groups also exceeded the analogues from the control by the amount of hemoglobin in the blood by 19.32 and 15.48%, respectively. After completing the 2nd course of drug administration, horses from the experimental groups of 2006 and 2002 were superior to their control analogues in hemoglobin content by 19.26 and 16.90%, respectively.

When using Vetom 3.22, the hemoglobin content in the blood of experimental animals was higher than the analogues from the control throughout the entire period of research. The increase in hemoglobin in the blood of horses of the experimental groups was within the physiological norm. Maintaining a high level of hemoglobin in the blood of horses in the period before the competition creates optimal conditions for animals to undergo massive physical exertion. A high level of hemoglobin promotes activation of gas exchange function in tissues and a decrease in oxygen starvation. Maintaining a high concentration of hemoglobin in the blood of experimental animals after competition also stimulates metabolic functions in muscle tissues, contributing to a faster recovery.

When studying the effect of the drug on the urea content in the blood serum of animals of the experimental group of 2006, it was found that after the completion of the first course of the drug, the urea content decreased by 0.52%, and in the control group it increased by 23.76%, relative to the initial data . After the competition, the performance in horses of the experimental and control groups increased by 4.19 and 5.24%, respectively. After completion of the 2nd course of drug administration, the urea content in animals of the experimental group decreased by 11.58%, and in the control group increased by 6.80%. Thus, when using the drug for horses born in 2006, there is a decrease in the content of urea in the blood serum (Table 3).

In horses of the experimental group of 12 years of age, after completing the 1st course of the drug, the urea content decreased by 12.21%, and in analogues from the control - by 4.13%. After the competition, an increase in serum urea in animals of the experimental and control group was recorded by 0.81 and 19.03%, respectively. After completion of the 2nd course of the drug administration, the urea content in the blood serum of horses of the experimental group decreased by 7.30%, and in the control - increased by 7.54%. Thus, when using the drug, the level of urea in the blood of the experimental groups decreases.

In horses of the experimental group born in 2006, prior to the use of the drug, the serum creatinine concentration was higher than in the horses of the control group by 7.77% (Table 4). After completion of the 1st course of drug use, this indicator in animals of the experimental group decreased by 4.52%, and in the control group increased by 1.96%. After the competition, the serum creatinine content in the horses of the experimental group decreased by 8.15%, and in the control group it increased by 6.33%. After completing the 2nd course of drug administration, the indicators of the experimental and control groups decreased by 1.88 and 1.38%, respectively. During this period, the content of creatinine in the blood serum of experimental horses was lower than analogues from the control by 13.73%.

In horses born in 2002, similar changes in serum creatinine were noted. In animals of the experimental group, this indicator was higher than in the control by 19.23% before the start of research (table. 4). After completing the 1st course of drug administration in experimental animals, this indicator decreased by 4.40%, and in the control group it increased by 6.31%. After the competition, the content of serum creatinine in horses of the experimental group increased by 2.00%, and in analogues from the control it decreased by 0.80%. After completing the 2nd course of the drug, the indicators of the experimental and control groups decreased by 6.62 and 6.21%. Thus, the content of creatinine in blood serum with the use of Vetom 3.22 decreases, which indicates the elimination of products of enhanced metabolism after active physical activity in competitions in a short time.

The increase in the content of red blood cells and hemoglobin in the blood of experimental horses before the start of the competition creates optimal conditions for transferring extreme loads during the competition. During the second course of drug use during the recovery period after exercise, the drug maintains a high content of red blood cells and hemoglobin, which provides the necessary level of metabolic processes in the muscles to accelerate the recovery period. A decrease in the level of urea and creatinine when using the drug is important, especially during the recovery period. This physiological state allows the products of active metabolism to be eliminated from the body in a short time, which means it reduces the recovery period of muscle tissue and the whole organism.

Claims (3)

A way to increase efficiency and reduce the recovery period of sports horses, including the use of a non-doping drug, characterized in that the animals are given a physiological probiotic preparation Vetom 3.22 with food in two courses before and after the competition, moreover: first course: 1 time per day at a dose of 50 μl / kg, 5 days in a row, then 5 applications 1 time in 2 days; second course: 1 time per day at a dose of 50 μl / kg, 3 cycles of 3 days with an interval of 3 days.

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