RU2599556C1 - Method for stimulating growth of meristematic potato plants in vitro - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: invention relates to agriculture. Meristematic plants are sprayed with 0.1 % solution of PABA, whereto 0.1 % of Fitolavin biocomposition is added, at temperature 20 to 25 °C, and during repeated spraying in phases 3-4 of leaves, 0.2-0.3 % potassium humate is added to solution.

EFFECT: invention allows to accelerate multiplication process in meristematic plants and increase number of mini tubers.

1 cl, 1 tbl, 1 ex

Description

The invention relates to the field of agriculture, in particular to seed production of potatoes, and may find application in the propagation of potatoes by the vegetative method in a greenhouse.

There is a method in which cultivated potato plants are cultivated by grafting on a nutrient medium of the Murasige-Skoog type, obtaining meristem plants — regenerants and planting plants in the ground (patent No. 2487532, published 12.12.2011, IPC A01Y 40/0, A01G 7/00) .

However, the known method is complicated by the fact that before planting in the soil, the plant regenerates are exposed to various temperatures, which allows to increase the cold resistance of plants by 55% and resistance to the nematode. But this slows down the growth and development of plants in vitro.

There is also known a method in which the propagation of meristemic plants is carried out by notching the apical part of its alienation and treatment with a solution of thiourea (patent No. 2415558, published November 17, 2009, IPC A01G 7/00, A01G 1/00, C05C 9/00).

The disadvantage of this method is that the propagation process is based on an incision of the apical part, processing of the shoots under different light and temperature conditions, which complicates the method.

The closest technical solution is the method in which the stimulation of the sprouts is carried out with silicon-containing fertilizer pre-moistened with 0.2% aqueous solution of iodine and 0.1% selenium (patent No. 2479983, published 04/27/2013 IPC A01G 7/00, A01G 1/00 , A01H 4/00).

The disadvantage of this method is that when propagating mini-tubers are removed from the vessels, supplementing the substrate with growth stimulants, which multiplication factor and accelerate the process of obtaining seed complicates the method.

The technical result is a simplification of the method, an increase in material.

The technical solution consists in the fact that to stimulate growth in para-aminobenzoic acid of a 0.1% aqueous solution, the biological product Fitolavin is introduced at a temperature of 20-25 ° C, treating the planted tube meristem plants with this mixture and, after repeated processing, in a phase of 3-4 leaves , additionally, potassium humate is added to the solution at a concentration of 0.2-0.3%.

The method is as follows.

Phytolavin - VRK (water-soluble concentrate) contains a complex of streptotricin antibiotics.

This microbial composition has a fungicidal property, allows you to protect meristem plants from bacterial and fungal diseases.

An aqueous 0.1% solution of para-aminobenzoic acid (PABA) has a positive effect on the bacterial drug Fitolavin, enhancing its effect, without changing the structure and composition of the biological product.

Para-aminobenzoic acid is a phenotypic activator that does not change the genetic structure of potato seedlings, but has a positive effect on the used biological product Fitolavin.

Using PABA, Phytolavin easily penetrates the plant and, circulating in their tissues, interacts with the ribosomes of pathogenic bacteria, inhibiting protein synthesis and disrupting the reading of the genetic code of bacteriosis pathogens, thus allowing plants to resist the introduction of pathogens into them for a long time. The stimulating effect of Fitolavin on the growth and development of young shoots is noted, which warns them against diseases of bacteriosis, anthracnosis and other diseases.

Potassium humate contains up to 80% potassium and up to 15% sodium active complexes of humic acids and fulvic acids and about 5% water-soluble compounds of calcium, magnesium, phosphorus, silicon and other rare and rare earth elements of the periodic table, which are very necessary for fast and high-quality plant growth. Potassium humate is a necessary link between plants and soil cover, adapts and mobilizes the immune system of plants, adapting it to the environment. Humic acid and fulvic acids actively interact with various components of the soil, forming various water-soluble micro elements that, when mixed with PABA and Phytolavin, penetrate plant cells and interact with elements of the pot substrate. Considering the fact that PABA powder is soluble only in hot water (at 80 ° C), and after it has cooled to 20-25 °, 0.1% Phytolavin biological product has been introduced to preserve its composition and active substances.

Example. In vitro tube meristems were planted in pots and transferred to a greenhouse. After 6-8 days before the appearance of the first true leaves, plants were sprayed with a mixture of 0.1% PABA aqueous solution, which was prepared at the rate of 10 g per 1 liter of water. Water was heated to 80 ° C. After the solution cools down to 20-25 ° С, 10 ml (1 teaspoon) of Fitolavin biological product was added to an aqueous 0.1% PABA solution and pots were sprayed with plants. When 3-4 true leaves appear, spray the crops again, adding 2-3 teaspoons of potassium humate to the solution.

The results of the experiments are summarized in table.

Therefore, stimulation by Fitolavin dissolved in a 0.1% PABA solution and additional spraying of plants with the introduction of potassium humate into the solution provides an increase in the reproduction rate and accelerates the process of obtaining high-quality disease-resistant potato seeds.

Claims (1)

A method of stimulating the growth of potato meristemic plants in vitro, including stimulating regenerated plants with chemicals, increasing their reproduction rate, characterized in that 0.1% Fitolavin biological product is introduced into an aqueous 0.1% para-aminobenzoic acid solution at a temperature of 20-25 ° C, treating meristemic plants with this mixture and, when they are re-processed in a phase of 3-4 leaves, potassium humate is added to the solution in a concentration of 0.2-0.3%.