RU2582288C1 - Method for prolonged chemoprophylaxis of hemosporidial infections of sheep and goats - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: invention relates to veterinary medicine and is intended for prevention piroplasmoses of sheep and goats. Animals are administered an aqueous solution of DAC with glycerine in following ratio in 100 ml of solution, g: DAC - 5 g, glycerine - 15 ml and distilled water - 80 ml.

EFFECT: method effectively improves safety of sheep and goats from piroplasmoses disease.

1 cl, 1 tbl, 2 ex

Description

The invention relates to veterinary medicine, in particular veterinary protozoology, and can be used for the prevention of pyroplasmidoses (pyroplasmosis, francaiellosis and babesiosis) of sheep and goats.

A known method for the chemoprophylaxis of pyroplasmidoses of sheep and goats by using an aqueous solution of diamidine in combination with primaquine and a prolongator polyethylene glycol - 6000, at a dose of 5 ml / kg body weight, intramuscularly (see patent for invention No. RU 2173143 C2, A. Rashidov and. other).

Due to the lack of such drugs, to prevent the occurrence of outbreaks of blood-parasitic diseases of sheep and goats, it is necessary to treat pyroplasmidoses 5-6 times per season with excessive use of chemotherapeutic drugs, unnecessary labor costs, reduced productivity and animal welfare.

The aim of the present invention is to increase the duration of the chemopreventive action of DAC in combination with glycerol in pyroplasmidoses of sheep and goats. This goal is achieved by the fact that instead of an aqueous solution of diamidine, an aqueous solution of DAC is used in combination with glycerin. The solution is prepared sterile in the following way: 80 ml of sterile distilled water, preheated to a temperature of 37 ° C, is added to the flask, 5 g of DAC granules (Granulatum "DAC"), a drug containing at least 99.2% ADV diminacene acetate, are successively dissolved. then add 20 ml of glycerol (1,2,3-trioxypropane, propanetriol-1,2,3) - the simplest representative of trihydric alcohols. It is a viscous transparent liquid, constantly mixed, subject to the rules of asepsis and antiseptics.

In sheep and goat farms unsuccessful for pyroplasmidoses, 2 groups of 40 animals each with an average live weight of 35-50 kg were selected in the flock. In order to prevent these diseases, an aqueous solution of DAC was used in combination with glycerin.

In order to exclude parasitic carriage in experimental animals, peripheral blood smear microscopy was performed and blood serum was examined in CSCs, parasite intensity was determined in blood smears by counting the affected red blood cells in 100 fields of view of the microscope.

Example 1. The first group of animals was injected intramuscularly with 2.3 ml per 40 kg of live weight, a solution of the drug prepared according to the specified recipe,

DAC - 5 g

Glycerin - 10 ml

Distilled water - 85 ml.

The results of observations showed that within 20 days there were no cases of the disease.

When examining animals for 20-21 days of administration of water-glycerin solutions of neosidine, 9 animals with clinical signs of babesiosis were identified. Microscopic examination of blood smears confirmed the diagnosis.

Example 2. The second group of experimental animals was injected intramuscularly at a dose of 2.3 ml per 40 kg of live weight, a solution of the drug prepared according to the following composition:

DAC - 5 g

Glycerin - 15 ml

Distilled water - 80 ml.

The observation results showed that when examining for 31-32 days after the introduction of a 5% aqueous-glycerin solution of DAC, 3 individuals with symptoms of the disease were isolated. Microscopic examination of blood smears confirmed the diagnosis.

Within 35 days, no signs of pyroplasmidosis were found in animals. Every 35 days during the pyroplasmidosis season (April to July), treatments were repeated.

Accounting and analysis of material, labor costs for the basic and proposed method for the chemoprophylaxis of pyroplasmidoses of sheep and goats showed that the new option requires 3 livestock treatments per season of the disease.

In addition, the proposed method does not reduce the productivity of animals associated with stress, violation of the feeding regime and, along with maintaining well-being in animal diseases, contributes to a significant relief of the work of veterinarians, livestock breeders and the prevention of environmental pollution by pesticides, their accumulation in the animal body and preservation human health.

Studies have also found that with a single intramuscular injection of these drugs, at the rate of 3.5 ml / 100 kg of live weight, in a very short time the parasitic response in the body of sick animals is sharply reduced. If prior to treatment of animals, up to 65 blood parasites were counted in blood smears in 100 fields of view of the microscope, while viewing blood smears 5-7 days after treatment, blood parasites were not detected. This dose provides prophylaxis of pyroplasmidoses for 35 days. In case of an outbreak of the disease, treatment of animals in the season (April to July) can be repeated 2-3 times, with an interval of 35 days. The results of experiments to test prophylactic efficacy in pyroplasmidoses using this formulation are given in the table.

List of references

1. Dyakonov L.P. and other parasitic diseases of farm animals. Moscow, Agropromizdat, 1985, p. 21-27.

2. Rashidov. A.A et al. Method for prolonged chemoprophylaxis of pyroplasmidoses of sheep and goats; Patent RU No. 2173143 C2 dated 09/10/2001.

3. Brains I.E. Veterinary formulation / I.E. Brains. - M .: Kolos, 1966 .-- S. 99-105.

4. Chervyakov D.K., Evdokimov P.D., Vishker A.S. / Medicines in veterinary medicine; Chervyakov D.K., Evdokimov P.D., Vishker A.S. - M .: Kolos. 1977, p. 441.

5. Altukhov A.M. and other Reference veterinarian. M .: Kolos, 1996, S. 293-299.

6. Zhulenko V.N. et al. General clinical and veterinary formulation. Directory. - M .: Kolos, 1988, p. 478.

7. Shevtsova A.A. and other parasitology. - M.: Agropromizdat, 1985, p. 334-336.

Claims (1)

A method for prolonged chemoprophylaxis of pyroplasmidoses of sheep and goats, comprising administering DAC to them, characterized in that the animals are injected with a solution prepared in the following manner in 80 ml of sterile distilled water, 5 g of DAC granulate are dissolved, then 15 ml of glycerol is added and mixed.