RU2583139C1 - Method for prolonged chemoprophylaxis of hemosporidial infections of cattle - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: invention relates to veterinary medicine and is intended for prevention of piroplasmoses in cattle. Animals are administered a solution consisting of neosidine - 7 g, glycerine - 20 ml and distilled water - 73 ml.

EFFECT: method improves safety of cattle from piroplasmoses.

1 cl, 1 tbl, 1 ex

Description

The invention relates to veterinary medicine, in particular veterinary protozoology, and can be used for the prevention of cattle pyroplasmidoses (pyroplasmosis, francaiellosis and babesiosis).

A known method for the chemoprophylaxis of pyroplasmidoses in cattle by using an aqueous solution of diamidine in combination with primaquine and a prolongator polyethylene glycol 6000 at a dose of 5 ml / 100 kg live weight (see patent for invention No. 2259821 C2, A. Rashidov and others). The disadvantage of this method is that at present most of the drugs are not produced, including diamidine, polyethylene glycol, the latter is produced abroad and is not available from veterinary specialists of the Republic of Dagestan. Therefore, the problem of finding highly effective drugs for the prevention of cattle pyroplasmidoses available for peasant farms and private farmsteads remains an urgent task. Due to the lack of such drugs, in order to prevent the occurrence of outbreaks of blood-parasitic diseases in cattle, it is necessary to process 8-9 times per season with an overrun of acaricidal and chemotherapeutic drugs, unnecessary labor costs, and a decrease in the productivity and preservation of animals.

The aim of the present invention is to increase the duration of the chemopreventive effect of neosidine-5% in combination with glycerin in bovine pyroplasmidoses. This goal is achieved by the fact that instead of an aqueous solution of diamidine, a 7% aqueous glycerol solution of neosidine is used. The solution is prepared sterile in the following manner. 73 ml of sterile distilled water, preheated to a temperature of 37 ° C, are added to the flask, 7 g of neosidine, a medicine containing at least 45% diminazene diaceturate and 55% phenazone, are successively dissolved, after which 20 ml of glycerol is added (1,2,3 -trioxypropane, propanetriol-1,2,3 - the simplest representative of trihydric alcohols), which is a viscous transparent liquid, then they are constantly mixed. Observe the rules of asepsis and antiseptics.

To work out the optimal scheme in catastrophic dysfunctional cattle pyroplasmidoses, 3 groups of experimental animals of 50 animals each with an average live weight of 150-250 kg were selected.

In order to exclude parasitic carriage in animals taken in the experiment, microscopy of smears of peripheral blood was performed and blood serum was examined in CSC. In ill animals, the intensity of parasitemia was determined in blood smears by counting the affected red blood cells in 100 fields of view of the microscope.

Example 1. The first group of experimental animals was injected intramuscularly with 2.5 ml per 50 kg of live weight solution of the drug prepared according to the following recipe:

neosidine 7 g glycerol 10 ml distilled water 83 ml

The observation results showed that within 20 days of the disease among animals of this group was not.

When examining animals on days 21-22 after treatment, 6 animals with clinical signs of pyroplasmidosis were isolated. Microscopic examination of blood smears confirmed the diagnosis of the disease.

Example 2. The second group of experimental animals was injected intramuscularly at a dose of 2.5 ml per 50 kg of live weight, a solution of the drug prepared according to the following composition:

neosidine 7 g glycerol 15 ml distilled water 78 ml

The observation results showed that within 30 days among animals of this group there were no cases of animal disease with pyroplasmidoses. When examining 31-32 days after the introduction of a water-glycerin solution of neosidine, 3 heads with symptoms of the disease were allocated. Microscopic examination of blood smears confirmed the diagnosis.

Example 3. The third group of experimental animals in order to increase therapeutic efficacy and prolong the action of the drug neosidine was introduced 5% concentration of 2.5 ml per 50 kg of live weight. The solution was prepared according to the following recipe:

neosidine 7 g glycerol 20 ml distilled water 73 ml

Within 45 days, no symptoms of pyroplasmidosis were detected in the experimental animals. On the 46th day, 2 cattle heads with signs of pyroplasmidosis were detected. Accounting and analysis of material and labor costs for the basic and proposed methods for the chemoprophylaxis of cattle pyroplasmidoses showed that, according to the new version of the preparations, it is consumed 4-5 times less. In addition, the proposed method does not reduce the productivity of animals associated with stress.

Studies have also found that with a single intramuscular injection of a solution of the specified composition at the rate of 5 ml / 100 kg of live weight, the parasitic reaction in the body of sick animals sharply decreases in a short time.

If before treatment of animals, up to 165 blood parasites were counted in blood smears in 100 fields of view of the microscope, then when viewing blood smears 5-7 days after the treatment, no blood parasites were detected. This dose provides prophylaxis of pyroplasmidoses for 45 days. In case of an outbreak of the disease, treatment of animals during the season of the disease (from April to September) can be repeated 3-4 times with an interval of 45 days. Experiments on testing the prophylactic effectiveness of prolonged solutions of neosidine preparations for cattle pyroplasmidoses in comparison with diamidine and a prolonged solution of diamidine in combination with primaquine on polyethylene glycol - 6000 are presented in the table (see table).

List of references

1. Dyakonov L.P. and other parasitic diseases of farm animals. - M .: Agropromizdat, 1985 - S. 21-27.

2. Brains I.E. Veterinary recipe / I.E. Brains. - M .: Kolos, 1966 - S. 99-105.

3. Rashidov A.A. et al. Method for the prevention of pyroplasmosis and cattle francaiellosis. Copyright certificate SU No. 1541816 A1, dated 8/10/1989

4. Rashidov A.A. Method for prolonged chemoprophylaxis of pyroplasmidoses (pyroplasmosis, babesiosis and teileriosis) in cattle. Patent for invention RU №2259821 C2, dated 10.09.2005,

5. Chervyakov D.K., Evdokimov P.D., Vishker A.S. Medicines in veterinary medicine // Chervyakov D.K., Evdokimov P.D., Vishker A.S. - M .: Kolos. 1977 - S. 441.

Claims (1)

A method for prolonged chemoprophylaxis of bovine pyroplasmidoses, including administering neosidine to them, characterized in that the animals are injected with a solution prepared by the following method, 7 g of neosidine are dissolved in 73 ml of sterile distilled water, then 20 ml of glycerol is added and mixed.