RU2499600C1 - Formulation with stabilised oxidation-reduction potential - Google Patents

Abstract

FIELD: medicine, pharmaceutics.

SUBSTANCE: declared invention refers to a formulation with a stabilised oxidation- reduction potential which possess antimicrobial, antifungal, wound-healing, virucidal and antiviral activity. The formulation contains an electrochemically activated aqueous solution of sodium chloride and potassium iodate salts wherein the oxidation-reduction potential is stabilised by acetic acid, lactic acid, alkali or alkali earth acetate or lactate in the amount not less than 0.0001 wt %.

EFFECT: invention provides stabilising the positive oxidation-reduction potential that prolongs the storage length of the formulation benefits.

3 cl, 9 tbl, 9 ex

Description

The invention relates to the field of electrochemistry, physical chemistry, chemistry of solutions and is used to stabilize electrochemically activated aqueous solutions and / or water-containing raw materials with positive redox potential, and may find application in areas such as sanitation, medicine, veterinary medicine, pharmaceutical industry, food industry, cosmetics, balneology, agriculture, fish farming and other fields of technology.

State of the art

Known for universal purpose (disinfection, pre-sterilization cleaning, sterilization), an electrochemically activated aqueous solution, which includes acetic acid in an amount of 1 volume percent. The solution has a high Еh = + 900 - (+ 1000) mV potential, the value of which decreases to Eh = + 500 - (+ 600) mV during the day. The specified solution, having a high microbicidal activity, does not cause metal corrosion during processing by immersion, is effective under conditions of high organic load, which is favorable when processing medical devices. The specified solution has a slight smell of chlorine, which is an important advantage of the solution. After 6 hours, the indicated solution is not recommended, and after 24 hours the active substances of the indicated electrochemically activated solution are almost completely mutually neutralized (see Bakhir V.M. et al. “STEL installations: history and new horizons”, article in the journal “Medical Alphabet” M. 2007, No. 2, p. 32-34).

It is known that electrochemically activated aqueous solutions with lower mineralization and high, Eh> + 800mV, redox potential, exhibit higher biocidal properties, with less corrosion, which, however, reduces their stability during use (O.I. Sukhova, VB Rovinskaya, V. I. Prilutsky "Assessment of the stability of the physicochemical parameters of an anolyte electrochemically activated solution (ANC)" Collection. First international symposium. Electrochemical activation in medicine and biology. M. Mosk and 27.10.1997 year. s.67-68).

A known method of obtaining a composition according to patent RU 2189217, published on 10.06.2001, containing an electrochemically activated aqueous solution, characterized by a positive redox potential, including a solution of salts of sodium chloride and potassium iodate. Increasing the stability and the value of Eh of the redox potential of the solution to Eh> +1100 - (+ 1200) mV provides potassium iodate at a low (10 or more times) level of sodium chloride in the solution.

The disadvantage of the composition obtained by the above method is that after 10-12 days, the composition loses its high redox potential (Eh) and, together with the potential, its useful qualities, which is unacceptable for the creation of industrially applicable disinfectant, antiseptic and other means , including hygienic and therapeutic purposes, as well as other products.

Disclosure of invention

The technical result to which the present invention is directed is to stabilize the redox potential of the composition, which is an electrochemically activated aqueous solution and / or water-containing raw material, characterized by a positive redox potential, including a solution of salts of sodium chloride and potassium iodate, in order to increase the term preservation of useful qualities of the specified composition.

The specified technical result is achieved by adding to the composition, which is an electrochemically activated aqueous solution and / or water-containing raw material, characterized by a positive redox potential, including a solution of salts of sodium chloride and potassium iodate, acetic acid or lactic acid, or acetic acid salt, or lactic acid salt alkali or alkaline earth metals, or mixtures thereof in an amount of not less than 0.0001% of the mass.

The composition with stabilized redox potential according to the invention can be a composition of therapeutic and therapeutic purposes, including a drug for external, intracavitary, intravaginal, vaginal, intravenous, intramuscular, intraperitoneal, subcutaneous, intradermal, skin and / or internal and other methods introduction; solution for correction of electrolyte and / or acid-base and / or redox balance, dialysis solution, liquid for storing contact lenses, eye drops, base for a drug that affects various types of metabolism, including carbohydrate metabolism, calcium phosphorus exchange, homeostasis, hematopoiesis, hemostasis; an agent that affects immunity, corrects antitumor therapy, antibiotic therapy used in gynecology, virology, otorhinolaryngology, dentistry, ophthalmology, proctology, narcology as a detoxifying agent; dermatology for external use; a disinfectant and / or antiseptic agent, an agent for treating dysbiosis, an inhibitory activity of pathogenic bacteria of the gastrointestinal tract, an anti-inflammatory agent, a detoxifying agent, an antimicrobial agent of various groups, an antiviral and virucidal agent, an anti-tuberculosis agent, an anti-fungal agent, agent used in gastroenterology and / or hepatology, bronchopulmonary agent, antiallergic agent, as well as physiological p target, parenteral agent for rehydration and / or detoxification, drug for correcting electrolyte and / or acid-base and / or redox balance disturbances, parenteral nutrition agent, multivitamin preparation with a complex of biogenic adaptogens, amino acid preparation, drug used for functional asthenia, a corrective food supplement, a plasma substitute and / or artificial blood substitute; dressings, including cotton-gauze dressing, bandage, cotton wool, hydrogel swab, collagen film, liquid collagen, algipore gel, sorbent based on coal, microcrystalline cellulose and / or polysaccharides, pectin, polypepam, zeolite, chitin and / gel, powder, solution; mouthwash, tooth elixir, paste, lotion, cream, water and / or water-oil extract of herbs, biogenic preparation, gel, aerosol, tampon, lotion, deodorant, wet sanitary towel, diaper, mask, shampoo, conditioner, medicine showing the properties of a disinfectant, antiseptic, preservative, virucidal and antiviral agents.

The composition with a stabilized redox potential according to the invention can be a finished food product, for example, table and medicinal mineral water, drinking water, and can also be a composition for protecting food products, including mineral and drinking water, drinks, meat, fish, vegetable, and / or fruit semi-finished products, minced meat, caviar, sausage and / or canned composition, confectionery, bakery products, pasta, means for protecting food products exhibiting the properties of a disinfectant, antiseptic, preservative, antiviral agent.

A composition with a stabilized redox potential according to the invention can be a balneological composition, including mineral water, mud, clay, silt, a balneological agent exhibiting the properties of a disinfectant, antiseptic, preservative, virucidal and antiviral agents.

A composition with a stabilized redox potential according to the invention may be a cosmetic composition, including, an oral rinse, a dental elixir, a paste, lotion, cream, an aqueous and / or oil-water extract of herbs, a biogenic preparation, a gel, an aerosol, a tampon, lotion, deodorant, wet sanitary napkin, cosmetic product exhibiting the properties of a disinfectant, antiseptic, preservative, virucidal and antiviral agents, as well as cosmetic raw materials from which next made cosmetics, including based on perfluorinated hydrocarbons, liposomes and microcapsules.

The composition with stabilized redox potential according to the invention can be a livestock composition, including feed and / or medicinal and drinking water for animals, drugs exhibiting the properties of a disinfectant, antiseptic, preservative, virucidal and antiviral agents, as well as raw materials for fish farming, such as, for example, preventive and medicinal products for fish farming, water for the aquarium.

A composition with a stabilized redox potential according to the invention can be a veterinary composition, including food, and / or drink, a drug exhibiting the properties of a disinfectant, antiseptic, preservative, virucidal and antiviral agent.

The composition with a stabilized redox potential according to the invention can be a composition of a means for protecting against diseases of crops and agricultural products, including cereal crops and seeds, both cereal crops and berries, fruits, feed crops, other fruit and vegetable products, a means to protect crops and agricultural products, showing the properties of a disinfectant, antiseptic, preservative, antiviral agent.

This does not exhaust the list of applications of the composition of the present invention and other options for its use as therapeutic, veterinary, agricultural, cosmetic and other types of raw materials and finished compositions are possible.

Acetic acid, lactic acid, acetic acid salt, lactic acid salt of alkali or alkaline earth metals, and mixtures thereof are known (see, for example, V.L. Beloborodoye, S.E. Zurabyan, A.P. Luzin, N.A. Tyukavkina Organic Chemistry. M. 2002. p. 455 and p. 559, as well as, for example, see F.L. Kalinin, V.P. Lobov, V.A. Zhidkov. Handbook of Biochemistry. Naukova Dumka. Kiev - 1971 p. 626, -627, p. 633-634).

The acetic acid, lactic acid, acetic acid salt, lactic acid salt of alkali and alkaline earth metals, and mixtures thereof are an integral part of the human body, animals, plants and other organisms, are not toxic, and are approved for use as a component of therapeutic and prophylactic purposes, as preservatives and as a component of food products (see, for example, a book edited by prof. G.N. Khlyabich. “Infusion therapy and clinical nutrition.” Firm Frez Nius AG-FRG. 1992 p. 92-93; and also see, for example, Nechaev A.P., Traubenberg S.E., Kochetkova A.A. et al. "Food Chemistry" St. Petersburg, GIORD, 2001, p. 251-254, p. 414-421).

As a result of adding to the composition, which is an electrochemically activated aqueous solution and / or water-containing raw material, characterized by a positive redox potential, including a solution of salts of sodium chloride and potassium iodate, acetic acid or lactic acid, or acetic salt, or alkaline lactic acid salt, or alkaline earth metals, or mixtures thereof in an amount of not less than 0.0001% by mass, the oxidation-reduction potential of the composition is stabilized, allowing l useful properties of the composition, for at least a year when stored in the dark, without special temperature storage conditions, or allows you to save useful properties under conditions of accelerated storage, suggesting an elevated storage temperature corresponding to two years of storage of the composition at a temperature of 20 ° C, and also allows sterilize the composition according to the invention at a temperature of 105-120 ° C.

The stabilization of the redox potential of the composition, which is an electrochemically activated aqueous solution and / or water-containing raw material, characterized by a positive redox potential, including the mixture of salts and organic acids of the present invention, leads to the prevention of microbiological damage to crops and agricultural products, including , grain crops, fruits and vegetables when processed with the specified composition, leads to prevent microbiological damage to various surfaces, including when processing the specified composition of these surfaces, and also protects people, animals and animal products, plants and fruits and vegetables, other food products, from viruses, bacteria, fungi, molds with the introduction of the composition according to the invention to a human or animal and / or consumption of the composition by a human or animal and / or application of the composition to human tissue, to animal tissue, or processing of the composition of the present invention to plants, and can also ensure the safety of tissues and organs intended for transplantation in a viable state by suppressing microbial contamination.

The useful qualities of an electrochemically activated aqueous solution and / or water-containing raw materials with the redox potential indicated according to the invention are confirmed by the applicant experimentally, by biological testing, as well as instrumental methods.

The composition of the present invention can be used in various industries, such as, for example, medicine, balneology, cosmetics, pharmaceuticals, veterinary medicine, agriculture, food industry.

The composition with a stabilized redox potential, which is an electrochemically activated aqueous solution and / or water-containing raw materials of the present invention, has a combination of unique biologically and chemically active properties, manifested in:

- bactericidal, bacteriostatic, antifungal, anti-mold, virucidal, antiviral;

- manifested in other useful qualities, while maintaining them for at least a year, provided that they are kept in a sealed container.

The composition in the context of the present invention can also be any currently known and obtained in the future electrochemically activated aqueous solution and / or water-containing raw materials, characterized by a positive redox potential, which contains a solution of salts of sodium chloride and potassium iodate, in which acetic acid is added or lactic acid, or acetic acid salt, or lactic acid salt of alkali or alkaline earth metals, or mixtures thereof in any combination.

Identification of the composition of the present invention includes:

- the use of a spectrophotometer, for example, KFK-3 or KFK-3-01, or other measuring equipment, in order to determine the presence of electrochemical activation, such as, for example, determination of hypochlorous acid, hypochlorite ion, active oxygen compounds by measuring optical density solution on the indicated spectrophotometers, at a wavelength of 590 nm in a cuvette with a layer thickness of 20 mm using the standard method described in the registration certificate ZhPS-0022150 dated 10.27.2006 to the Anolyte neutral therapeutic agent ny.

- the use of a pH meter, or an ionomer, such as, for example, "pH-340", "EV-74", etc., in order to measure the redox potential of the composition according to the invention, and, in the presence of a positive redox potential composition, the composition is further analyzed for the presence of sodium chloride and iodates in the composition, for example, by chromatographic or spectrophotometric, or other kind of analysis. In the presence of sodium chloride and potassium iodate, the composition is further analyzed for the presence of the organic acids indicated in the present invention or salts of these organic acids or a mixture thereof, for example, by chromatographic or spectrophotometric or other analysis to determine the acetic acid or lactic acid indicated in the present invention acid, or acetic acid salt, or lactic acid salt of alkali or alkaline earth metals, or mixtures thereof.

In the case of the presence in the composition of the present invention, including an electrochemically activated aqueous solution and / or water-containing raw materials, characterized by a positive redox potential, a solution of salts of sodium chloride and potassium iodate, acetic acid or lactic acid, or acetic acid salt, or alkaline lactic acid salt, or alkaline earth metals, or mixtures thereof in any combination, the composition has all the essential features of this invention and is it.

The composition in the context of the present invention may also be any composition of synthetic, artificial, animal or vegetable origin, currently known and obtained in the future, containing an electrochemically activated aqueous solution and / or water-containing raw material, characterized by a positive redox potential, including a solution of chloride salts sodium and potassium iodate, acetic acid or lactic acid, or acetic acid salt, or alkaline lactic acid salt, or alkaline Gmina Jemielno metals, or mixtures thereof in any combination.

The preparation of this composition is predominantly done by adding to the aqueous solution and / or water-containing raw materials, characterized by a positive redox potential, including sodium chloride and potassium iodate salts, acetic acid or lactic acid, or acetic acid salt, or lactic acid salt of alkali, or alkaline earth metals, or mixtures thereof in any combination according to the invention. The specified method ensures the long-term safety of bactericidal, bacteriostatic, antifungal, anti-mold, virucidal, antiviral and other useful qualities of the specified composition.

Obtaining a composition includes various optional operations. These include dilution and thickening operations. The dilution operation can be actually performed by both the manufacturer and the user of the composition. Dilution can lead to a decrease in the redox potential of the composition, and thickening can lead to an increase in the redox potential of the composition. Obtaining the composition does not require special restrictions on dilution and thickening, provided that this does not reduce the preservation of the specified redox potential of the composition of the present invention.

Other optional operations possible in this invention include the steps of adding and / or mixing any optional suitable component.

The optional ingredients of the composition of the present invention are:

- all ingredients that may be optionally added by the manufacturer of the composition in order to give the composition an improvement in presentation, smell, taste, aroma, consistency or substance added to the composition in order to accelerate or facilitate the conduct of technological processes, as well as all substances that do not affect preparation technology and / or manifestation of the action of cosmetic and medicinal products based on the specified composition of the present invention;

- color stabilizers (fixers), such as, for example, salts of nitrous and nitric acids, for example, nitrites (NaNO ₃ and / or KNO ₃ ) or / and nitrates (NaNO ₂ or KNO ₂ );

- flavorings, such as, for example, natural essential oils and extracts (oleoresins), fir, eucalyptus, etc .;

- acidifiers, such as, for example, fruit acids, in particular citric, succinic, hydrochloric, sulfuric, phosphoric, salts of these acids, etc .;

- consistency regulating substances, such as, for example, polyoxyethylene sorbitan esters (E 432 ... E 436), ammonium salts of phosphatidyl acid (E442), mono and diglycerides of fatty acids (E 471), phospholipids, sucrose and fatty acid esters (E 473) , other glycerol esters (E 474 ... E 477), sodium lactylate (E481

(1) and calcium (E 482), esters of sorbitan, SPAN, bi (E 491 ... E 496);

- thickeners and gelling agents, such as, for example, acid polysaccharides with residues of uronic acid, acid polysaccharides with residues of sulfuric acid, neutral polysaccharides, acid hydrocolloids with residues of uronic acid, for example, tragacanth E 413 and gum arabic E 414, as well as neutral compounds. For example, locust bean gum E410 and guar 412, as well as agar and carrageenan, alginic acid and its salts, as well as modified starches (E 1400 ... 1405, E1410 ... 1414 E 1420 ... 1423, E 1440, E 1442, E 1443, E 1450), cellulose esters E 461 ... E 467, including CMC (carboxymethyl cellulose), polysaccharides of microbial origin, for example, xanthan E 415, gellan gum E 418, alginic acid E 400 and its salts E 401 ... E 404, pectin E 440; hyaluronic acid and its salts;

- water-retaining agents, such as, for example, glycerin, sorbitol, invert sugar and other sugar-like substances, agar, alginates, pectins;

- film formers, thickeners and gelling agents, as well as polymer dispersions, glycerin, mono and diglycerides of fatty acids, natural and synthetic waxes, lanolin, paraffin;

- acidity regulators, such as, for example, acids, alkalis, buffer salts;

- emulsifying salts such as, for example, yeast, ammonium, bicarbonate

sodium;

- substances that facilitate filtering, such as, for example, adsorbents, flocculants (among which, in particular, cellulose, kieselguhr, perlite);

- brighteners, such as, for example, agar, activated carbon, carrageenan, cellulose, gelatin, fish glue, charcoal, dried chicken egg protein, kaolin, potassium hexacyanoferrate, kieselguhr, phytic acid, polyvinylpyrrolidone, tannin, sodium pectate, fulleran;

- encapsulating agents, such as, for example, gelatin, casein, gum arabic, pectin, carboxymethyl cellulose, fats and polymers, as well as mixtures of emulsifiers and hydrocolloids, and, as plasticizers, glycerin, sorbitol, gum and sugar;

- means for tabletting, such as, for example, fillers, separators, water-retaining agents, adsorbents, accelerators and dissolution inhibitors, stabilizers, dyes and flavoring substances:

a) fillers - starch, amylose, microcrystalline cellulose, dicalcium phosphate, lactose, magnesium oxide, mannitol, polyglycols, sugars and sweeteners, sucrose, sorbitol, mannitol, grape sugar, or water-soluble ethylene glycols;

b) separators (lubricants) - surfactants, powdered cellulose, paraffin, cetyl alcohol, stearic acid, stearates, talc, polyethylene glycols;

c) dissolution accelerators - modified starches, powdered cellulose, microcrystalline cellulose, methyl and ethyl cellulose, croscaramellose, alginic acid, insoluble calcium alginate, pectin, tragacanth, agar, sodium alginate;

d) adsorbents - starches, milk sugar, cellulose, kaolin, bentonite, highly dispersed pyrogenic silicic acid;

d) dissolution inhibitors - solid paraffin stearate, cocoa butter, large quantities of carboxymethyl cellulose, polyethylene glycol and polyvinylpyrrolidone;

- dispersing agents (dispersants), for example, solubilizers and instantizers;

- antioxidants and shielding gases, for example, sorbic acid and its salts; benzoic acid and sodium benzoate; methyl, ethyl, propyl esters of n-hydroxybenzoic acid; formic acid; sulfur dioxide and sodium and potassium sulfites; sodium o-phenylphenol and sodium o-phenylphenolate; diphenyl; carbon dioxide; nitrogen.

The preferred composition in accordance with the present invention is an electrochemically activated aqueous solution and / or water-containing raw material, characterized by a stabilized redox potential, which has a positive value, including sodium chloride and potassium iodate salt, acetic acid or lactic acid, or acetic acid as mandatory components salt, or lactic acid salt of alkali or alkaline earth metals, or mixtures thereof in any combination, upon receipt of the cat At least one additional optional mixing operation or a thickening operation or a dilution operation is allowed, during which an ingredient selected from the group consisting of dyes, color stabilizers (fixatives), bleaches, flavors, flavor and aroma enhancers, and cookers is added. salts, sugars, proteins, acids, alkalis, buffer mixtures, salt substitutes, sugar substitutes (sweeteners), emulsifiers, thickeners and gelling agents, preservatives, antioxidants, densifiers, moisture-retaining agents, anti-caking agents, film-forming agents, acidity regulators, antifoam agents and anti-foaming agents, emulsifying salts, disintegrants, filtering aids, clarifiers, extractants, carriers, diluents, solvents, separators, desiccants, cooling and freezing agents, contributing agents vital microorganisms, propellants, enzymes and enzyme preparations, hydrolysis and inversion catalysts, dispersing agents and rugih groups with optional ingredients, whose presence in the composition of the present invention is not mandatory, but that provide the composition a particular commercial importance.

The specified composition according to the invention can be produced by the above methods, which, however, does not limit its production by these examples.

The best embodiments of the invention

The invention is illustrated by the following, non-limiting examples. The following examples are preferred, intended only to confirm the feasibility of the invention.

Example 1

This example confirms the well-known position that an electrochemically activated solution, characterized by a positive redox potential, including a solution of salts of sodium chloride and potassium iodate, retains the initial positive value of the redox potential of the solution for no more than 20 days.

An aqueous solution of sodium chloride salt at a concentration of 0.0005-0.02% with the addition of potassium iodate at a concentration of 0.0004-0.0006% in a mixture with running distilled water is subjected to electrochemical activation in an installation for the electrochemical synthesis of activated detergent disinfectants and sterilizers "STEL" type solutions. Eh (redox potential) of the resulting solution is +1100 - (+ 1200) mV, CSE (millivolt at a silver chloride reference electrode), at a pH in the range of 2.3-8.0. Next, the solution is poured into sterile opaque glass bottles and stored in a refrigerator at a temperature of t = + 4 ° C. After 20 days, the bottles open and measure the value of the redox potential. Its value is Eh = + 760 - (+ 820) mV, ChSE. Thus, the drop in the positive potential is 440 mV, CSE. It is known that, at the indicated potential value, the bactericidal properties of the solution sharply, hundreds of times, decrease (see the article by V. I. Prilutsky “The survival limits of isolated cells in electrochemically active (ECA) media”, collection, 2nd international symposium “Electrochemical activation in medicine, agriculture, industry ”, M., October 28-29, 1999). Similar experiments were performed repeatedly with similar results. Similar results were obtained by other experimenters.

Example 2

This example proves that acetic acid or lactic acid, or acetic acid salt, or lactic acid salt of alkali or alkaline earth metals, or their mixture stabilizes the positive redox potential of an electrochemically activated solution, including a solution of salts of sodium chloride and potassium iodate.

An aqueous solution of sodium chloride salt at a concentration of 0.02% of the mass, with the addition of potassium iodate to it at a concentration of 0.0006% of the mass. mixed with running distilled water with the initial characteristics Eh = + 260 mV, the CSE is subjected to electrochemical activation in an installation for electrochemical synthesis of activated detergent disinfectant and sterilizing solutions of the STEL type. At the output, the value of the redox potential of the solution is Eh = + 1100 mV, CSE. The resulting electrochemically activated solution, characterized by a positive redox potential, Eh = + 1100 mV, HSE, pH = 6.5, is transferred to 36 vials of 3 vials in each group (a total of 12 groups), add the acetic acid and lactic acid indicated according to the invention , sodium acetic acid, sodium lactic acid, potassium acetate, potassium lactic acid, calcium acetate, calcium lactic acid, magnesium acetate, magnesium lactic acid, and mixtures thereof in various concentrations in each group of vials, per hour tn, 0.0001% of the mass, 0.1% of the mass, 10.0% of the mass and dissolve. The vials are closed with caps, sterilized in an autoclave at 105 ° C for 20 minutes. After the sterilization procedure, these bottles with the indicated solutions according to the invention are placed in a thermostat at a temperature of 50 ° C. After 91 days, the vials are opened and the values of the redox potential are measured. According to the current instruction I-42-2-82, experimental storage at 50 ° C for 91 days corresponds to 2 years of storage at a temperature of 20 ° C. The potential drop does not exceed 5-15% of the initial level.

Comparison is made with an electrochemically activated, similar to experimental, control solution, characterized by a positive redox potential Eh = + 1100 mV, CSE, including a solution of salts of sodium chloride and potassium iodate. The specified control solution loses potential, the value of which for 20 days of storage at t = 50 ° C reaches Eh = + 270 mV, which is 307% of the initial level.

Thus, this experiment confirms that the composition of the present invention has a stabilized redox potential.

Table 1 Stabilization of the Redox Potential of an Electrochemically Activated Solution by the Composition of the Present Invention Component Name The concentration of the components in the composition according to the invention 0.0001% of the mass. 0.1% of the mass. 10.0% of the mass. Eh in 91 days pH after 91 days Eh in 91 days pH after 91 days Eh in 91 days pH after 91 days Experimental storage at a temperature of 50 ° C, which corresponds to 2 years of storage at a temperature of 20 ° C, according to instructions I 42-2-82 on work to determine the shelf life of drugs based on the method of “accelerated aging” at elevated temperature. Acetic acid +900 6.3 +1050 6.5 +1000 6.5 Sodium Acetate +780 6.4 +970 6.5 +970 6.5 Potassium acetate +790 6.5 +940 6.2 +990 6.5 Calcium Acetate +820 6.7 +900 6.7 +970 6.5 Magnesium Acetate +870 6.5 +990 6.5 +970 6.5 lactic acid +720 6.9 +870 6.8 +880 6.8 lactic acid sodium +680 7.1 +820 6.9 +830 6.9 potassium lactate +690 7.2 +830 6.9 +840 6.8 calcium lactic acid +710 6.9 +840 6.9 +810 7.1 lactic magnesium +730 6.8 +850 6.9 +870 6.8 Acetic acid + sodium acetic acid + lactic acid + sodium lactic acid +830 6.7 +950 6.4 +980 6.4 Calcium Acetate + Calcium Lactate + Acetic Acid + Magnesium Acetate + Magnesium Lactate +840 6.4 +990 6.5 +1000 6.2 CONTROL SOLUTION Initial Eh = + 1100 pH = 6.5. After 20 days of storage at 50 ° C, Eh = + 270 pH = 7.3

Example 3

This example demonstrates the antimicrobial and antifungal activity of an electrochemically activated solution, characterized by a positive redox potential,

stabilized according to the present invention.

Commercial considerations determine the choice of components and their concentration when conducting the following experiment with the composition of the present invention.

The experiment was carried out as follows: an aqueous solution of sodium chloride salt at a concentration of 0.02% mass, with the addition of potassium iodate at a concentration of 0.0006% mass in a mixture with running distilled water with the initial characteristics Eh = + 260 mV, CSE is subjected to electrochemical activation in the installation for electrochemical synthesis of activated detergent disinfectant and sterilizing solutions of the "STEL" type. At the output, the value of the redox potential of the solution is Eh = + 1100 mV, CSE. The obtained, electrochemically activated solution, characterized by a positive redox potential Eh = + 1100 mV, HSE, pH = 6.5, is transferred to dark glass vials and stabilized with acetic acid and sodium acetic acid at a total concentration of 0.05% by weight in this solution, and sterilized at t = 105 ° C for 20 minutes. These vials are placed on accelerated aging in an autoclave at a temperature of 50 0 C and incubated for 91 days. After 91 days, the bottles are opened and at the same time:

- measure the values of the redox potential. According to the current instruction I-42-2-82, experimental storage at 50 ° C for 91 days corresponds to 2 years of storage at a temperature of 20 ° C. The potential drop does not exceed 10% of the initial level;

- produce studies of the antimicrobial activity of the composition of the present invention.

The purpose of the study was to assess the degree of antimicrobial activity of the above composition of the present invention, containing an electrochemically active solution, characterized by a positive redox potential Eh = + 1100 - (+ 990) mV, CSE, including a solution of salts of sodium chloride and potassium iodate, in which the reduction potential is stabilized by a mixture of acetic acid with sodium acetate. The concentration of the components is 0.05% by mass. The hydrogen value is pH = 6.0-6.5.

The study used 18 types of microorganisms, including 7 strains of gram-positive cocci: Staphylococcusaureus, Enterococcusfaecialis, three strains of streptococci (Str.viridans, Str. Agalactiae, Str.pyogenes), Gardnerellavaginalis, fungi of the genus Candidaalbicans, and strain Sporus Gram-negative microorganisms: Escherichiacoli, Pseudomonausaeruginosa, Klebsiellapneumonia, Klebsiella oxytoca, Shugtlladisenteriae, Shugtlla flexnery, Salmonellatyphimurium, Salmonella enteritidis, Neisseriagonorrhoeul Proteusmi Proteusmi

All test strains taken for research were obtained from the International collection of microorganism strains of GISK named after L.A. Tarasevich. In addition to museum reference strains, highly active clinical strains of microorganisms were used in the experiments.

The following nutrient media are used in the work: nutrient agar Muller-Hinton (MullerHintonagar), ThayerMartinBase medium with horse serum for gonococci and gardnerella, as well as Saburo agar for growing fungi of the genus Candida.

Research method: in all experiments, the methodology described in the Instruction for the determination of the bactericidal properties of disinfectants No. 739068 of 05/06/1968, of the All-Union Scientific and Research Institute of Disinfection and Sterilization is used.

Of all the test strains taken into the work of the above-mentioned microorganisms, suspensions of ten-fold dilutions containing 10 ⁹ 10 ⁷ , 10 ⁶ CFU / ml are prepared.

In the microbial suspension of these concentrations of test strains, the test composition of the present invention is introduced in a volume ratio of 1:10. The exposure time is from 0.5 to 5 minutes.

In parallel, in order to control cell growth in a nutrient medium in the absence of the composition of the present invention, appropriate control samples are set.

In addition, for a semi-quantitative assessment of the growth of microorganisms, 0.05 ml of the composition of the present invention is sown on nutrient Muller Hinton agar, Thayer Martin Base medium, as well as on Saburo agar to assess the growth of p. Candida fungi. Petri dishes with seeding were incubated at a temperature of 37 ° C for 18 hours. Microbial growth or lack thereof is estimated by the number of bacterial or fungal cells grown. The experiments are carried out in triplicate reproduction. The results of these studies are presented in table No. 2. In parallel with the evaluation of the antimicrobial effect of the composition of the present invention, the redox potential of the corresponding sample was recorded.

From the tabular data it is seen that in all experimental samples containing suspensions of the test strains and the composition of the present invention, 100% inhibition of the growth of all microorganisms is observed (with the exception of the spore bacillus Bacillus cereus, where partial inhibition of growth is observed). At the same time, in control samples (in the absence of the composition of the present invention), 100% full growth of seeded test strains was detected during the indicated exposure time.

The data obtained indicate that the test composition of the present invention, containing an electrochemically activated solution having a positive value of the redox potential, inhibits the growth and development of a wide range of microorganisms, including clinical strains with a high degree of drug resistance, belonging to a wide variety of species and to groups. A bactericidal effect was obtained on 5 strains of staphylococcus, including Staphylococcus aureus; 3 strains of E. coli, including hemolytic E. coli; 4 strains of Pseudomonas aeruginosa, 3 strains of streptococci, 2 strains of Klebsiella, 2 strains of Shigella, 2 strains of Proteus, 1 strain of gonococcus and gardnerella, as well as 2 strains of yeast-like fungi of the genus Candida.

table 2 Bactericidal properties of electrochemically activated solutions stabilized by the composition of the present invention Genus and species of microorganisms The number of microbial cells in the experimental and control sample (CFU / ml) Exposure time min Eh (redox potential) The number of surviving microbial cells in the sample Experienced * Control Staphylococcusaureus 10 ⁹ 10 ⁷ , 10 ⁶ 0.5-5 +990 0,0,0 10 ⁹ 10 ⁷ , 10 ⁶ Enterococcusfaecialis 10 ⁹ 10 ⁷ , 10 ⁶ 1-5 +990 0,0,0 10 ⁹ 10 ⁷ , 10 ⁶ Esherichiacoli 10 ⁹ 10 ⁷ , 10 ⁶ 1-5 +990 0,0,0 10 ⁹ 10 ⁷ , 10 ⁶ Klebsiellapneumonia 10 ⁹ 10 ⁷ , 10 ⁶ 1-5 +990 0, 0, 0 10 ⁹ 10 ⁷ , 10 ⁶ Klebsiella oxytoca, 10 ⁹ 10 ⁷ , 10 ⁶ 1-5 +990 0, 0, 0 10 ⁹ 10 ⁷ , 10 ⁶ Proteusmirabilis 10 ⁹ 10 ⁷ , 10 ⁶ 1-5 +990 0, 0, 0 10 ⁹ 10 ⁷ , 10 ⁶ Proteus vulgaris. 10 ⁹ 10 ⁷ , 10 ⁶ 1-5 +990 0, 0, 0 10 y 10 10 " Candidaalbicans 10 ⁹ 10 ⁷ , 10 ⁶ 0.5-5 +990 0, 0, 0 10 ⁹ 10 ⁷ , 10 ⁶ Streptococcusagalactiae 10 ⁹ 10 ⁷ , 10 ⁶ 1-5 +990 0, 0, 0 10 ⁹ 10 ⁷ , 10 ⁶ Streptococcus viridans 10 ⁹ 10 ⁷ , 10 ⁶ 1-5 +990 0, 0, 0 10 ⁹ 10 ⁷ , 10 ⁶ Pseudomonausaeruginosa 10 ⁹ 10 ⁷ , 10 ⁶ 1-5 +990 0, 0, 0 10 ⁹ 10 ⁷ , 10 ⁶ Salmonellatyphimurium 10 ⁹ 10 ⁷ , 10 ⁶ 1-5 +990 0, 0, 0 10 ⁹ 10 ⁷ , 10 ⁶ Salmonella enteritidis 10 ⁹ 10 ⁷ , 10 ⁶ 1-5 +990 0,0,0 10 ⁹ 10 ⁷ , 10 ⁶ Shugtlladisenteriae 10 ⁹ 10 ⁷ , 10 ⁶ 1-5 +990 0, 0, 0 10 ⁹ 10 ⁷ , 10 ⁶ Shugtlla flexnery 10 ⁹ 10 ⁷ , 10 ⁶ 1-5 +990 0, 0, 0 10 ⁹ 10 ⁷ , 10 ⁶ Neisseriagonorrhoeae 10 ⁹ 10 ⁷ , 10 ⁶ 1-5 +990 0, 0, 0 10 u10u, 10 6 Gardnerellavaginalis 10 ⁹ 10 ⁷ , 10 ⁶ 1-5 +990 0, 0, 0 10 ⁹ 10 ⁷ , 10 ⁶ Bacilluscereus 10 ⁹ 10 ⁷ , 10 ⁶ 1-5 +990 10 ⁴ 10 ³ , 10 ² 10 ⁹ 10 ⁷ , 10 ⁶ Note: * An experimental sample with a content of 0.05% of the mass. mixtures of acetic acid and sodium acetic acid

Example 4

An example demonstrating an increase in the sensitivity of bacteria to antibiotics after their cultivation in a composition with stabilized oxidative potential of the present invention and proves the possibility of their combined use in medicine, sanitation and veterinary medicine.

Using antibiotics as chloramphenicol, kanamycin, amoxicillin, nalidixic acid, amikacin, gentamicin and ciprofloxacin, the antibiotic resistance of Staphylococcus aureus, Salmonella, Shigella, and Klebsiella was tested. The method of diffusion of drugs into nutrient agar is used. For the treatment of bacteria, the above antibiotics are used, dissolved in the composition of the present invention, which is pre-diluted with distilled water in a ratio of 10 parts of water per 1 part of the composition of the present invention to pH = 7.2, mineralization 190 mg / l and Eh = + 750mV, CSE (experience). Processing is carried out for 1 minute. A water-salt solution prepared on the basis of sodium chloride dissolved in distilled water with ph = 7.2, mineralization 190 mg / L and Eh = + 290 mV, CSE is taken as a control sterilized by boiling for 40 minutes. The following results were obtained:

Salmonellasp. - the strain changed from resistant to sensitive to gentamicin and nalixidic acid and from moderately sensitive to sensitive to ampicillin and ciprofloxacin;

Shigellasp. - the strain has changed from resistant to sensitive to ampicillin, gentamicin and from moderately sensitive to sensitive to ciprofloxacin amikacin, kanamycin;

S.aureus - increased sensitivity to ampicillin; amikainu;

Klebsiella pneumoniae - increased sensitivity to amikacin.

Table 3 shows the percentage increase in the zone of inhibition of growth of m / organisms in the composition of the present invention compared with the control.

Table 3 The degree of enhancement of bacterial sensitivity (in%) salmonella shigella Klebsiella staphylococcus Ampicillin 26 44 17 22 Amikain eighteen 38 34 eighteen Nalidixic acid 42 33 eleven 3 Gentamicin 47 40 6 eleven Kanamycin eighteen 35 12 8 Chloramphenicol 6 17 5 3 Ciprofloxacin 28 36 17 10

This example proves the effect of increasing the sensitivity of the microorganism to antibiotics dissolved in the composition of the present invention. Thus, the contact of a microorganism with antibiotics dissolved in the indicated composition, characterized by a positive redox potential, insufficient to destroy the microorganism, nevertheless leads to an increase in the effectiveness of antibiotic treatment methods by increasing the sensitivity of microorganisms to them. The antibiotics indicated in this example do not limit the combined use of the composition of the present invention with other antibiotics, disinfectants, and other drugs used in medicine, sanitation, and veterinary medicine that are currently or in the future.

Example 5

An example that demonstrates the therapeutic effect of the composition of the present invention in experiments on a model of purulent-inflammatory process in rabbits and indicates that the composition can be used independently in medicine, sanitation and veterinary medicine as a means that accelerates the healing process compared to known means.

On 5 rabbits, experimental foci of the wound suppurative-inflammatory process are reproduced, followed by a study of the effect of the composition of the present invention on the wound healing process. Rabbits are applied with a scalpel symmetrically across the back, on both sides of the spinal column, three linear wounds of the same size, penetrating the skin, subcutaneous retina, superficial fascia and partially muscle. In total, 30 artificially created wounds are received. All wounds are then infected with a staphylococcal suspension of Staphylococcus aureus containing 10 ⁹ microbial bodies in 1 ml.

Three rabbits are treated with wounds using an electrochemically activated solution characterized by a positive redox potential, the value of which is Eh = + 1050mV, CSE stabilized with acetic acid and sodium acetic acid at a total concentration of 0.05% by weight in this solution. The composition was sterilized at t = 105 ° C for 20 minutes. For two rabbits, wounds are treated with physiological saline.

Treatment begins after the development of a purulent-infectious process in the wounds. The experimental design approaches the real conditions of the clinical treatment of infected wounds. The composition of the present invention moisten gauze wipes. Dressings are made taking into account the setting of the experiment and the composition used in the experiment. Dressings are made with copiously moistened napkins 4 times a day. Dressings moistened with saline are done at the same frequency. The experiments are carried out taking into account the rules of asepsis. During the experiment, wounds are examined and the pH value in the wounds is determined.

As a result of the experiment, the following data were obtained: in wounds treated with the composition of the present invention, there is a rapid restoration of a neutral or close pH (6.9-7.4), which is observed on the 2-3 day of the experiment. Clinical cleansing of wounds, reduction of pus, the appearance of good, juicy granulations and the filling of wounds with granulation tissue are noted. Swelling around wounds is reduced 3-4 days earlier than in wounds treated with saline. The healing of wounds treated with the composition of the present invention occurs on days 9-12, while the healing of wounds treated with saline occurs on days 16-19.

The use of this composition of the present invention is effective both in the first, and in the second and third phases of the wound healing process, at the stages of fibroblast proliferation and vascular growth, collagen fibrillogenesis, maturation and fibrous transformation of granulation tissue, and scar reorganization.

This example proves that the composition of the present invention not only inhibits the growth and development of microorganisms, but also accelerates the healing process and can be used not only as a therapeutic composition, but also as a cosmetic composition for the restoration of the skin after peeling (mechanical, chemical , cryopilling, etc.) and cosmetic operations that exhibit the properties of an antiseptic and a regenerating agent.

Example 6

This example proves that the composition of the present invention increases the safety of raw meat and indicates that the composition can be used as a means to protect food from microbiological contamination, without causing oxidative damage to phospholipids in food.

The following experiment was conducted on the impact on the safety of meat products (beef, pork) of the composition of the present invention in order to study the possibility of increasing the safety of meat products. The exposure time is 5 minutes.

The experimental procedure is as follows: 5 kg meat samples were cut from the carcasses of frozen animals, in the amount of 8 samples, thawed and soaked in the composition of the present invention and in the control solution, respectively. The exposure time is 5 minutes.

1. The composition of the present invention: an electrochemically activated aqueous solution, characterized by a positive redox potential, the value of which is Eh = + 1100 mV, CSE, at pH = 6.0, is stabilized with acetic acid and sodium acetate at their total concentration in the specified solution 0.1% of the mass.

2. Control solution: anolyte solution obtained on the STEL device at pH = 6.0, Eh = 1100 mV, CSE

After surface treatment of all meat samples, the microbial number on their surface decreased equally.

After 24 hours of keeping the control samples in the thermostat under aseptic conditions at t = 37 ° C in the depth of 4 meat samples treated with the control solution, the development of putrefactive processes was observed. It was found that the control solution leads to coagulation of surface meat proteins, which impedes the exchange of meat tissue with the environment, which led to putrefactive processes due to the development of sulfate-reducing bacteria in the depths of meat raw materials, which develop in the absence of oxygen in the medium. These bacteria are extremely dangerous to humans and animals.

After 24 hours of keeping 4 meat samples treated with the composition of the present invention in an thermostat under aseptic conditions at t = 37 ° C, the development of putrefactive processes was not observed for 5 days. The experiment is further interrupted. Further observations found that the development of putrefactive processes in the depths of raw materials during surface treatment of meat raw materials with the composition of the present invention occurs only in the absence of sufficient surface sterility, which is technologically simple to eliminate by increasing the redox potential of the composition of the present invention.

In the chemical analysis of proteins and fats of meat raw materials treated with a control solution and the composition of the present invention revealed:

- accelerated aging of fat components (oxidation of phospholipids, the accumulation of aging pigments) when treated with a control solution;

- the absence of aging of fatty components in the processing of raw materials with the composition of the present invention.

The meat treated with the composition of the present invention can be used for the preparation of minced meat, and the composition of the present invention can also be used as an aqueous component of the minced meat, which prevents the development of pathogenic microorganisms in the minced meat, in case they fall into the minced meat.

Thus, this experiment confirms that the composition of the present invention can be used as a means to protect the food product from microbiological infection, without causing oxidative damage to tissues.

Example 7

An example demonstrating protection against diseases of crops and agricultural products by the composition of the present invention.

The experimental procedure is as follows: infected with spores of Fusarium and smut smut and toxic grains of bread crops, in particular, wheat, barley, corn, peas, are uniformly sprayed with the finely divided composition of the present invention. Fine dispersion is created by ultrasonic treatment of the composition of the present invention and subsequent spraying of the composition under the film in a container with grain.

The composition of the present invention: an electrochemically activated aqueous solution, characterized by a positive redox potential, the value of which is Eh = + 1200 mV, CSE, at pH = 4.4, is stabilized with acetic acid at a concentration in the specified solution of 0.1% by weight.

Control: anolyte acid at Eh = + 1200, ChSE and pH = 4.5

The consumption of the composition of the present invention is 45 liters. per 1 ton of grain. The grain is kept in closed containers coated with a film. To disinfect only toxins, the composition according to the invention is used in a smaller amount - up to 25 l per 1 t. Grain

The effectiveness of the composition of the present invention on toxins of Fusarium and smut smut is shown in Table 1.

Table 4 The effectiveness of disinfectant solutions on toxins of Fusarium and smut. one 2 3 four Fusarium The composition of the present invention 3 hours 80% Anolyte control 45% Dust smut The composition of the present invention 3 hours 80% Anolyte control 55%

According to the table, both the composition of the present invention and the acidic anolyte have a neutralizing effect on fungal toxins. The disinfection efficiency of Fusarium toxins with the composition of the present invention is 1.8 times higher than that of anolyte and 1.45 times higher than smut toxins.

The effectiveness of the composition of the present invention and acid anolyte on spores of parasitic fungi at a dose of 45 l. per 1 t. of seeds is shown in Table 2.

Table 5 The effectiveness of disinfectant solutions on spores of parasitic fungi. Mushroom spores Type of solution Exposition Impact effect Aspergilus sp. The composition of the present invention 9 hours 95-100% sporocidal Anolyte sour 24 hours 95-100% sporocidal Fusarium sp. The composition of the present invention 9 hours 95-100% sporocidal Anolyte sour 24 hours 80% sporocidal

According to the table it can be seen that one hundred percent success in the fight against spore fungi has the seed treatment with the composition of the present invention in a dose of 45 liters. on

The necessary exposure can be maintained during storage of grain in closed containers.

The use of the composition of the present invention allows for a comprehensive and complete disinfection while reducing the time compared with currently known means.

Example 8

An example demonstrating the use of the composition of the present invention as a composition for balneological purposes and its positive effect on the state of the microflora of the gastrointestinal tract of volunteers. At the same time, the example indicates that the composition can be used to eliminate dysbiosis not only in humans, but also in animals.

In an experiment on a limited number of volunteers in the amount of 5 people, the effect of oral administration of the composition of the present invention having pH = 7.80 Eh = + 450 mV, CSE obtained by 100-fold dilution with mineral water of the initial, stabilized according to the present invention was studied, electrochemically activated solution, with parameters pH = 5.8 Eh = + 1100 mV, CSE and mineralization 190 mg / l. The mineralization of the composition of the present invention obtained in this way is 600 mg / L. Comparison of the action of the composition was carried out with the action of mineral water used to dilute the composition of the present invention, with parameters pH = 7.9-8.2 Eh = + 290, mV, CSE, also on a limited number of volunteers in the amount of 5 people.

After its intake in the amount of 600 ml per day for four weeks, a stable microflora composition was noted - the number of bifidobacteria averaged 10 ⁷ -10 ⁹ CFU / g, lactobacilli - 10 ⁶ -10 ⁷ , normal E. coli reached 10 ⁶ -10 ⁷ , the number of conditionally pathogenic intestinal microorganisms did not exceed 10 ¹ , there were no golden and other hemolytic species of staphylococci, microscopic fungi, aspergillus, conditionally pathogenic non-fermenting bacteria. The indicators of the immune system in the examined individuals were within the age limits. Seven people from the examined (4 people in the experimental group and 3 people in the control group), before taking the indicated composition, changes in the composition of the intestinal microflora of the following nature were revealed: a decrease in the number of lactobacilli (10 ³ -10 ⁴ ), bifidobacteria (10 ⁵ -10 ⁴ ) and full-fledged E. coli (10 ³ -10 ⁵ ), an increase in spore-forming microorganisms (10 ⁷ -10 ⁸ ) and fecal streptococci (10 ⁷ -10 ⁸ ), including those with hemolytic properties (10 ⁴ ). The number of opportunistic enterobacteria (Klebsiella) reached 10 ⁵ . After taking the composition of the present invention for four weeks, an increase to normal lactobacilli, bifidobacteria and full-fledged E. coli was noted. Klebsiella and hemolytic streptococcus were not allocated and the number of spore microorganisms decreased to 10 ³ .

In addition, pseudomonads and candida were eliminated from the intestinal tract, which were determined in small quantities by volunteers before receiving the specified composition of the present invention

Researchers V.D., G.Ya., I.V., who took the indicated composition for one month, when examining the intestinal tract, revealed a stable composition of microflora, despite the fact that pronounced clinical signs of dysbacteriosis were objectively observed prior to its administration ( alternation of constipation and diarrhea, flatulence, pain along the large intestine).

The investigated V.P. before taking this composition, the amounts of lactobacilli (10 ⁴ ) and Escherichia coli (10 ⁴ ) were reduced, conditionally pathogenic Klebsiella 10 ⁵ and Staphylococcus aureus - 10 ² , Clostridia - 10 ^{8 were} isolated. Four weeks later, with the intake of a stabilized composition, the following indicators of intestinal microflora were noted -

About mm

the number of lactobacilli increased to 10, full-length Escherichia coli to 10, the number of conditionally pathogenic Klebsiella decreased by two orders of magnitude, and by four orders of clostridia

In the control group, changes in the composition of microflora are not pronounced and do not go beyond the confidence interval, indicating a change in the composition of microflora compared with the initial composition.

Thus, the composition with the stabilized redox potential of the electrochemically activated solution of the present invention normalizes the unbalanced composition of the human intestinal microflora and can be used as a balneological agent to eliminate pathogenic microflora that causes symptoms of dysbiosis.

Since dysbacteriosis is widespread not only in humans, but also in animals, the composition of the present invention can be a composition of animal husbandry, which can help rid animals of dysbiosis by suppressing pathogenic intestinal microflora, and can be used in animal husbandry and veterinary medicine due to its effectiveness and insignificant cost of its production. Thus, this example proves that the composition can be used both as a balneological agent exhibiting the properties of an antiseptic, and as a means of animal husbandry, and as a veterinary prescription.

General conclusions to examples 3-8.

Conducted by studies on the use of the composition of the present invention with Eh = + 750 to +1200 mV, CSE, hydrogen pH = 4.4-7.8 and mineralization mainly at the level of 190 mg / l, on the vital activity of microorganisms of different families, the following were revealed:

1 The composition of the present invention, containing an electrochemically activated aqueous solution having a positive redox potential, inhibits the growth and development of a wide range of microorganisms, including clinical strains with a high degree of drug resistance, belonging to a wide variety of species and groups. A bactericidal effect was obtained on 5 strains of staphylococcus, including Staphylococcus aureus; 3 strains of E. coli, including hemolytic E. coli; 4 strains of Pseudomonas aeruginosa, 3 strains of streptococci, 2 strains of Klebsiella, 2 strains of Shigella, 2 strains of Proteus, 1 strain of gonococcus and gardnerella, as well as 2 strains of yeast-like fungi of the genus Candida. The number of microbial cells in the experimental and control sample (CFU / ml) is from 10 ⁹ to 10 ⁶ . The overwhelming majority of the 100% bactericidal effect is observed after 1 min, in some cases at 10 ⁹ (CFU / ml), the bactericidal effect is observed after 30 seconds of exposure. This example proves that the composition of the present invention can be a composition with an independent therapeutic and therapeutic effect.

2. The contact of the microorganism with antibiotics dissolved in the specified composition of the present invention, characterized by a positive redox potential, insufficient to destroy the microorganism (Eh = + 750 mV, CSE), leads to an increase in the sensitivity of microorganisms to antibiotics, which proves the possibility of the joint use of drugs preparations and composition of the present invention, and also proves the possibility of enhancing the effects of known drugs with the composition of the present zobreteniyu.

3. Processing by soaking for 5 minutes in the composition of the present invention thawed meat completely inhibits the reproduction and development of microorganisms on the surface of the meat, and at the same time does not lead to accelerated aging of meat phospholipids, does not lead to autolysis and coagulation of meat proteins, which is completely inhibits the development of putrefactive processes in the depths of the tissue array. This example proves that the composition of the present invention can be a composition of a product for protecting food products, an example of which is presented in the present experiment.

4. Processing the composition of the present invention of grain contaminated with spores of Fusarium and smut smut leads to the complete destruction of toxins of fungi and the destruction of their spores within 3 to 9 hours, provided that the processed products are stored under a film, which allows for comprehensive and complete disinfection while reduction of time compared with currently known means. Thus, this example confirms that the composition of the present invention may be a composition for protecting crops and agricultural products.

5. After treatment with the composition of the present invention of cut and infected wounds of Staphylococcus aureus, containing 10 ⁹ microbial bodies in 1 ml, a rapid restoration of a neutral or close pH (6.9-7.4) in the wounds is observed, which is noted in 2- 3 day experiment. Clinical cleansing of wounds, reduction of pus, the appearance of good, juicy granulations and the filling of wounds with granulation tissue are noted. Swelling around the wounds is reduced 3-4 days earlier than in wounds treated with saline. The healing of wounds treated with the composition of the present invention occurs on days 9-12, while the healing of wounds treated with saline occurs on days 16-19. The use of this composition according to the present invention is effective both in the first, and in the second and third phases of the wound healing process, at the stages of fibroblast proliferation and vascular growth, collagen fibrillogenesis, maturation and fibrous transformation of granulation tissue, and scar reorganization. This example proves that the composition of the present invention not only inhibits the growth and development of microorganisms, but also accelerates the wound healing process and can be used not only as a therapeutic composition, but also as a cosmetic composition for the restoration of the skin after peeling procedures (mechanical, chemical, cryo-peeling, etc.) and cosmetic operations, showing the properties of an antiseptic and a regenerating agent.

6. A balneological agent, such as, for example, mineral water produced on the basis of the composition of the present invention, by diluting the composition 100 times with mineral water, or produced in another way, such as, for example, by electrochemical treatment of mineral water, with its stabilization according to of the present invention, normalizes the composition of the human intestinal microflora by suppressing pathogenic and conditionally pathogenic microflora, against the background of which bifido and lactobacilli, which are useful for humans, are activated century microflora. Since dysbacteriosis is widespread not only in humans, but also in animals, the composition of the present invention can be a composition of animal husbandry, which can help rid animals of dysbiosis by suppressing pathogenic intestinal microflora, and can be used in animal husbandry and veterinary medicine due to its effectiveness and insignificant cost of its production. Thus, this example proves that the composition can be used both as a balneological agent exhibiting the properties of an antiseptic, and as a means of animal husbandry, and as a veterinary prescription.

Example 9

The present example demonstrates the virucidal and antiviral activity of the composition of the present invention by the example of hepatitis C virus and at the same time confirms that the composition is not toxic.

We used a cytopathogenic strain of hepatitis C virus (HCV) belonging to genotype 1b. The strain was isolated from the blood serum of a patient with chronic viral hepatitis C, identified as hepatitis C. Infectious doses of HCV equal to 10.0 TCD 50/20 μl were used in the work.

The cultures of transplantable green monkey kidney cells, clone No. 6 (Vero-6), highly sensitive to the cytopathogenic effect of HCV, were obtained from the Laboratory of Virus Ecology, Institute of Virology named after D.I. Ivanovo RAMS. They were used as a one-day monolayer of cells grown in 24-well plastic panels. Vero-E6 cell cultures were grown on Eagle’s dual medium with 10% serum of calf embryo supplemented with glutamine and antibiotics (100 U / ml).

To titrate the residual infectivity of the virus, a transplantable pig embryonic kidney cell line (SPEV), also sensitive to HCV reproduction, was used. SPEV cell cultures were obtained from Narvak, Moscow. They were used as a one-day monolayer of cells grown in 96-well plastic cell culture panels. SPEV cell cultures were grown on medium 199 supplemented with 10% cattle serum and antibiotics.

Piglet testicle cell cultures (PTP) were also used in the work. PTP cell cultures were used as a one-day monolayer grown on 24 and 48-well panels on Eagle's minimal medium (HyClone, USA) supplemented with 10% calf serum, glutamine and antibiotics.

In this work, we used a composition with a redox potential of Eh = + 940 mV, CSE, pH of 6.9-7.2 and with a salinity of 170-190 mg / l. Before starting the experiment, 2 g of dry Needle medium (HyClone company) were carefully dissolved in a horizontal position in water by carefully rotating the bottle, after complete dissolution, the liquid medium was filtered slowly so that the filtrate layered on the wall of the tube. Then carefully 7% serum of calf embryo antibiotics were added to the filtrate. This medium containing the composition of the present invention served as a support medium as well as a nutrient medium for infected and uninfected cell cultures.

As a control experiment, the same cell cultures and virus were used, which were grown on a medium not containing the composition of the present invention. The following studies were carried out:

1. The study of the cytotoxic properties of the composition of the present invention.

For this purpose, Vero-E6, SPEV and PTP cell cultures were grown and incubated on a medium containing the indicated composition for 4 days. The results of the study of cell viability were compared with those in cell cultures grown on standard medium.

2. The study of the virucidal properties of the composition of the present invention. Virus-containing liquid and nutrient medium containing the composition of the present invention were combined in a ratio of 1: 9, respectively, and kept at 4 ° C for 1 hour and 24 hours. The control was a virus-containing liquid, connected in a 1: 9 ratio with a normal nutrient medium that did not contain the indicated composition. The results were taken into account after titration of the residual HCV infectious activity in the cell cultures of the experimental and control samples.

3. The study of the antiviral activity of the composition of the present invention.

The antiviral activity of the specified composition was investigated in anti-TBP cell cultures according to the study of the viability of infected cells grown on a normal medium and a medium containing the specified composition, and the concentration of the infectious virus produced by cells grown on a normal medium and a medium with the composition of the present invention

The following results were obtained.

4. The study of the cytotoxic properties of the composition of the present invention. The data were obtained that the medium containing the indicated composition does not have toxic properties and does not affect the viability and proliferative activity of uninfected Vero-E6, PTP and SPEV cell cultures. Moreover, data have been obtained indicating that cell cultures grown on a medium containing the indicated composition are characterized by a higher adhesive ability of the cell monolayer (the ability to attach to the bottom of the culture vial), which may also indicate greater viability of cells cultured under these conditions

5. The study of the virucidal properties of the composition of the present invention. These data are presented in table.6.

Table 6 Virucidal properties of the composition of the present invention Cell culture HCV titers in cell culture medium containing and not containing a composition with stabilized redox potential (log TCD50 / ml) Control environment Medium with stabilized redox potential lg HCV titer reduction Vero-E6 (24 hours) 11.5 8.5 3.0 SPEV (1 hour) 6.3 4.0 2,3 PTP (24 hours) 2,8 No. 1 - 0 2,8 No. 2 - 0.5 2,3

24 hours - incubation of the virus for 24 hours at + 4 C, 1 hour - for 1 hour under the same conditions; No. 1 and No. 2 are samples of the composition of the present invention.

As can be seen from the data in table 6, the exposure of the HCV-containing material in the medium containing the composition of the present invention for 24 hours at + 4 C, led to a decrease in the infectious activity of the virus for cell cultures of different origin by 2.8-3.0 lg ТЦЦ50. A slightly lower activity of water was manifested during exposure for 1 hour (a decrease in HCV titers by 2.3 lg TCC50). Thus, the data presented in table 1, indicate that the medium Needle, prepared on the composition of the present invention, is characterized by virucidal activity.

6. Antiviral properties of the composition of the present invention in relation to infection caused by HCV in cell cultures of anti-TB drugs.

Research data on the antiviral properties of water are presented in table. No. 7, 8.9.

Table 7 Viability of HCV infected PTP cell cultures cultured on medium containing the composition of the present invention The introduction of the medium containing the composition of the present invention immediately after infection of HCV cells. Experience Options % surviving HCV infected cells in repeated experiments on day 3 after infection but b at Experience No. 1 one hundred one hundred one hundred Experience No. 2 one hundred 75 one hundred Experience No. 3 fifty fifty fifty Cell control one hundred one hundred one hundred

Experience No. 1 - the use of the composition of the present invention under No. 1

Experience No. 2 - the use of the composition of the present invention under No. 2

Experience No. 3 - the use of standard distilled water; a, 6, c - repeated studies.

It was found that the growth of HCV-infected PTP cell cultures on a medium containing the composition of the present invention added immediately after infection of the cells leads, as a rule, to 100% cell survival. At this time, in control experiments by this day, 50% of HCV infected cells of the monolayer were killed. These data support the antiviral properties of this composition.

Table 8 Viability of HCV infected PTP cell cultures cultured on medium containing the composition of the present invention The introduction of the medium containing the composition of the present invention, 24 hours before infection of HCV cells. Experience Options % surviving HCV infected cells in repeated experiments on day 3 after infection but b at Experience No. 1 one hundred one hundred one hundred Experience No. 2 one hundred one hundred one hundred Experience No. 3 fifty fifty fifty Cell control one hundred one hundred one hundred

Experience No. 1 - the use of the composition of the present invention under No. 1

Experience No. 2 - the use of the composition of the present invention under No. 2

Experience No. 3 - the use of standard distilled water; a, b, c - repeated studies.

From the data of table 8 it is seen that the growth of HCV infected cultures of PTP cells on a medium containing the composition of the present invention, added 24 hours before infection of the cells, also leads to 100% cell survival. At this time, in control experiments by this day, 50% of HCV infected cells of the monolayer were killed. These data also support the antiviral properties of this composition.

Table 9 Antiviral activity of the composition of the present invention on a model of HCV infection in anti-TB drugs The time of adding medium containing a composition with a stabilized redox potential Hepatitis C virus titers (lg ТЦЦ50 / ml for SPEV cell cultures) in medium samples taken from PTP cell cultures on the 3rd day after infection Experience No. 1 Experience No. 2 Experience No. 3 aa bb AA BB AA bb lg HCV titer reduction At the time of infection 1,5 11.5 11.5 00.8 55.0 55,2 3,5-4,4 24 hours before infection 44.3 44.3 44.3 33.3 77.0 66.9 2.7-3.7 Note: Designations are the same as in tables 7 and 8.

In order to directly evaluate the antiviral effect of the composition of the present invention in anti-HCV-infected PTP cell cultures, on the 3rd day after infection, medium samples were taken and titrated on PTP cell cultures. The data of table 9 represent the results of titration of samples of the culture medium.

It has been shown that a composition with a stabilized redox potential has antiviral properties. In particular, it was found that this composition has the greatest antiviral activity in the case of cell treatment, both at the time of infection and 24 hours after infection of the HCV cell cultures with the composition of the present invention. In these cases, virus titers in cell cultures treated immediately after infection with the indicated composition decreased by 3.5-4.4 lg TCD50. The treatment of cells with the composition of the present invention 24 hours before infection of the cells, as a rule, also led to a significant antiviral effect of the specified composition (reduction of HCV titers by 2.7-3.7 lg TCD50).

In this way:

1. The composition with a stabilized redox potential of the present invention does not possess cytotoxic properties for Vero-E6, SPEV, PTP cell cultures for 4 or more days of cultivation.

2. The composition with stabilized redox potential of the present invention has virucidal activity against hepatitis C virus when exposed to HCV-containing fluid and Eagle’s medium prepared on the specified composition for 1 hour and 24 hours, HCV titers for cell cultures are reduced by 2.3-3.0 lg TSD50.

3. The composition with the stabilized redox potential of the present invention has antiviral properties and is able to reduce the production of HCV by infected cells by an average of 3.0-4.0 lg when the specified composition is added immediately after HCV adsorption on the cells or 24 hours before infection hepatitis C virus cells

Industrial applicability

A composition with stabilized redox potential is proposed, which is an electrochemically activated aqueous solution and / or a water-containing raw material, characterized by a positive redox potential, including a solution of sodium chloride and potassium iodate salts, in which the redox potential is stabilized by acetic acid or lactic acid, or acetic acid salt, or lactic acid salt of alkali or alkaline earth metals, or mixtures thereof. The proposed composition can find application in medicine, veterinary medicine, pharmaceutical industry, cosmetic industry, balneology, food industry, agriculture, fish farming and other fields of technology.

Claims (3)

1. The composition with a stabilized redox potential, with antimicrobial, antifungal, wound healing, virucidal and antiviral activity, which is an electrochemically activated aqueous solution of salts of sodium chloride and potassium iodate, characterized by a positive redox potential, characterized in that the redox potential stabilized with acetic acid, or lactic acid, or acetic acid salt, or lactic acid with oly alkali or alkaline earth metals, or their mixture in an amount of not less than 0.0001 wt.%. 2. The composition according to claim 1, in which sodium acetate, or potassium acetic acid, or sodium lactic acid, or potassium lactic acid, or mixtures thereof are used as alkali metal salts. 3. The composition according to claim 1, in which the salts of alkaline earth metals are calcium acetate or magnesium acetate, or calcium lactate, or magnesium lactate, or mixtures thereof.