RU2189217C1 - Method for producing aqueous antiseptic solution for locally treating abscesses and phlegmons - Google Patents

Abstract

FIELD: medical engineering. SUBSTANCE: method involves subjecting 0.0005-0.02% aqueous sodium chloride solution with 0.0004-0.0006% potassium iodate being added in mixture with flowing distilled water to electrochemical activation in an installation for performing electrochemical synthesis of activated detergent disinfection and sterilization solutions. Direct current with triple current shape asymmetry and frequency of 10 to 50 Hz is used. Installation operation time, current density and water pressure are set so that acidous cathode anolyte is produced at pH=2.3-2.5 or neutral cathode anolyte is produced at pH=7.5-8.0 having redox potential equal to 1100-1200 mV and oxidant concentration C_ox=25-150 mg/l. EFFECT: normalized processes in wound surface.

Description

 The invention relates to medicine, in particular to purulent surgery in the treatment of abscesses and phlegmon, as well as burn purulent wounds.

At present, hydrogen peroxide H ₂ O ₂ , hydrogen peroxide, is widely used as an aqueous antiseptic solution. A 3% aqueous solution of hydrogen peroxide is approved for use in medical practice and has positive bactericidal properties.

 A known method of producing an antiseptic - hydrogen peroxide, described in the textbook "Pharmaceutical Chemistry", the authors G. Melentyev et al., M., Medicine, 1993, p. 105, 106 (1).

 This method of producing hydrogen peroxide is as follows.

 An acidic solution of ammonium sulfate is taken and electrolyzed in a bath without a separation diaphragm, which would create anode and cathode spaces. The electrolysis of a solution of ammonium sulfate produces weak solutions of hydrogen peroxide. Then, more concentrated solutions are prepared by vacuum distillation.

 At the same time, a solution of hydrogen peroxide has a negative effect, namely, that it “burns” young sprout tissue of the wound surface. This is a serious contraindication for the treatment of wounds in the healing phase.

 In addition, the technology for producing hydrogen peroxide is quite complicated.

 Currently, in world medical practice there are no aqueous solutions of antiseptics that are equal in bactericidal to a solution of hydrogen peroxide, but do not have the negative properties noted above.

 The aim of the invention is to develop a method for producing an aqueous solution of an antiseptic having positive bactericidal properties similar to the properties of hydrogen peroxide, but not having the negative properties of the latter.

 According to the applicant, such an antiseptic is anolyte - an aqueous solution of peroxides, that is, atoms that are part of solutions of salts subjected to electrolysis, including hydrogen, but the concentration of peroxides is 100 or more times lower than in a 3% peroxide solution hydrogen.

 Obtaining this antiseptic is achieved by activating salt solutions during electrolysis in STEL-type plants.

 The solution of anolyte neutral cathodic and anolyte acid cathodic does not have the negative properties inherent in a 3% aqueous solution of hydrogen peroxide, but rather normalizes reparative processes in the wound surface.

 The goal is to increase the bactericidalness of the aqueous solution of the proposed antiseptic to an oxidation-reduction potential (REDOX) of not less than 1100-1200 mV and, on this basis, the possibility of effective treatment of abscesses and phlegmon with this antiseptic solution, which has a significantly high therapeutic effect, namely by introducing a solution of new halogens, for example, iodine compounds, which can reduce the total concentration of oxidizing agents in an antiseptic solution to 25-150 mg / l, but this increases the bactericidal effect A solution of antiseptic for a long period of action (up to 10-12 days).

 The specified goal is achieved as follows.

An aqueous solution of sodium chloride salt at a concentration of 0.0005-0.02% with the addition of potassium iodate to it at a concentration of 0.0004-0.0006% in a mixture with running distilled water is subjected to electrochemical activation in an installation for the electrochemical synthesis of activated detergent disinfectants and sterilizers solutions of the STEL type, using direct current having triple asymmetry of the current shape with a frequency of 10 to 50 Hz, and the installation time, current density and water pressure are set in such a way as to obtain an anolyte cathode at pH 2 , 3-2,5 or neutral anolyte cathode at pH 7.5-8.0 with a redox potential of 1100-1200 mV and a concentration of oxidants C _ox = 25-150 mg / L.

 As a close analogue of the proposed method for producing an aqueous solution of an antiseptic for the local treatment of abscesses and phlegmon, you can take the method of producing hydrogen peroxide according to the source of scientific and technical information (1).

The proposed method is as follows. A salt solution is prepared, for example, sodium chloride of 1.0-2.0% concentration, before electrolysis, into which a microdose of potassium iodate (KIO ₃ ) is introduced at a concentration of 0.04-0.06%. And in the final solution, after dilution in distilled water in a STEL installation, the concentration of sodium chloride salt will be brought to 0.0005-0.02%, and the concentration of potassium iodate salt will be brought to 0.0004-0.0006%. And this mixture of two salt solutions is subjected to electrolysis in the STEL installation, in the anode space of which the solutions are acidified to the specified parameters.

In this case, direct current is used, having a triple asymmetry of the current shape with a frequency of 10 to 50 Hz, and the installation operation time, current density and water pressure are set in such a way as to obtain an acid cathode anolyte at pH 2.3-2.5 or neutral anolyte cathodic at a pH of 7.5-8.0 with a redox potential of 1100-1200 mV and a concentration of oxidizing agents With _ox = 25-150 mg / L.

 The STEL installation is an installation for the electrochemical synthesis of activated detergents, disinfectants and sterilizing solutions STEL-104-120-01 (models 40-01) manufactured by AOOT NPO Ekran, is listed in 0028-96 dated 03.03.96 g Domestic and foreign disinfectants authorized by the RF Ministry of Health in Russia.

 To implement the proposed method, the STEL installation uses an improved power supply (RF certificate 14329 for a utility model), which provides new DC parameters, namely, triple current asymmetry with a frequency of 10 to 50 Hz.

и ион (IO₃)^-.Used in the proposed method, potassium iodate (KIO ₃ ) during dissociation in water forms two ions - a potassium ion

and ion (IO ₃ ) ^- .

At the same time, ion (IO ₃ ) is a very strong oxidizing agent, and during electrolysis in the STEL installation it does not break down into constituent elements, and retains high bactericidal properties of the solution, and increases the period of active concentration of oxidizing agents up to 10 days or more. And this is twice the recommended period in accordance with the Methodological Instructions of the Ministry of Health of the Russian Federation MU-17-12 of 02/14/1997 on the use of the neutral anolyte ANK produced in the STEL installation. See the literature by Ya. I. Turyan, "Redox reactions (see continuation of p. 3a on p. 4) and potentials in analytical chemistry", M., Chemistry. 1989, p. 195. 231-232 (2), as well as N.S. Akhmetov "General and inorganic chemistry", M., Higher School, 1998 p. 320 (3).

Potassium iodate (KIO ₃ ) is much more stable than chlorates and bromates, see source (2), p. 336.

Эта система сохраняет свой высокий окислительный потенциал, равный +1,653 В длительно, см. источник (3), с. 195.Therefore, the redox system represents

This system retains its high oxidation potential, equal to +1.653 V for a long time, see source (3), p. 195.

 Therefore, solutions containing a system with potassium iodate retain their oxidizing properties for a very long time (10 or more days).

 Thus, the use of potassium iodate and new DC parameters for the electrochemical activation of aqueous salt solutions provides high physicochemical parameters and also high bactericidal properties of the aqueous antiseptic solution obtained by the proposed method.

 Obtained by the flow method according to the proposed method, the solution at the outlet of the STEL installation is a neutral cathode anolyte (ANK) or an acid cathode anolyte (ACK), depending on the specified pressure parameters.

Then, the pH of the resulting solution is measured, REDOX potential and the concentration of oxidizing agents is determined by the iodometric method. With stable indicators (ACC) - pH 2.3-2.5 and (ANC) - pH 7.5-8.0. REDOX equal to 1100-1200 mV and the concentration of oxidizing agents With _ox , equal to 25-150 mg / l, the solution is poured into sterile glassware and transferred to dressings for local treatment of abscesses and phlegmon. The shelf life of the properties and effects of this antiseptic is at least 10 days.

 The proposed method for producing an aqueous solution of an antiseptic — a neutral cathodic anolyte (ANK) and an acidic cathodic anolyte (AKK) for the treatment of abscesses and phlegmon, can significantly expand the zone of bactericidal action of the AKK solution to the maximum possible zone of bacteria; see pic 29 - area of theoretically possible survival of microorganisms (Japanese version) from an article by V.I. Prilutsky "The limits of survival of isolated cells in electrochemically active (ECA) environments", collection, 2nd international symposium "Electrochemical activation in medicine, agriculture, industry", Moscow, October 28-29, 1999 (attached).

 In the proposed method, the concentration of sodium chloride is reduced in relation to the analog by 1000 times to 0.005%, as well as to the passport mode of the STEL installation by 10 times, which can significantly reduce the consumption of expensive chemically pure drugs in comparison with the analog, therefore, get cheaper , but significantly more effective antiseptic. In the proposed method, along with sodium chloride, potassium and calcium salts can be used.

 Prepared by the proposed method, the antiseptic solution has sterility for 10 days, has a 100% bactericidal effect on gram-positive and gram-negative microorganisms in all exposures according to the time of collection in various dilutions in a cubic centimeter. See the act of March 27, 1999 on conducting research work on the therapeutic effect of a neutral cathode anolyte solution (ANC) in the city clinical hospital for emergency medical care (GKBSMP-1) in Omsk (attached on 2 sheets).

 Obtained by the proposed method, the antiseptic solution was used in the treatment of phlegmon in the city clinical hospital 11 (GKB-11) in Omsk and confirmed high efficiency. The Act of August 31, 1999 on the clinical trials of activated solutions in the treatment of phlegmon of the maxillofacial region is attached on 2 sheets.

 In September 2000, repeated control tests of the proposed antiseptic solution were conducted in the city clinical emergency hospital (GKBSMP-1) also with positive results (attached on 3 sheets).

 The following are 3 examples.

 Example 1

 Patient K., born in 1934. He entered the Department of Oral and Maxillofacial Surgery (FMH) of the City Clinical Hospital on June 19, 1999, medical history 2244. The diagnosis is clinical: odontogenic phlegmon of the parotid chewing region on the right.

 Treatment: autopsy phlegmon; first, traditional treatment was carried out locally; the patient's condition worsened, during bacteriological examination of the wound discharge, Staphylococcus Proteus, Staphylococcus aureus, Candida st. , which according to the conclusion of the laboratory were insensitive to antibiotics. The threat of development of mediostenitis has increased. It was decided and obtained the patient’s consent (July 1, 1999) for the use of a solution of acid cathodic anolyte (ACC) in the treatment of wounds. The wound was locally treated according to the following procedure: after treatment with a 3% solution of hydrogen peroxide, the wound was washed with anolyte solution (ACC) jet from a syringe and abundantly moistened with a gauze swab, then a perforated catheter was placed on the bottom of the wound for irrigation with anolyte solution (ACC) through 1.5 -2 hours for 5-10 ml. Dressings were performed in the morning and evening. The catheter was replaced with a new one in a day.

 A bacteriological study conducted prior to the use of anolyte (ACC) on June 28, 1999 showed that St. was isolated from the wound. Proteus antibiotic insensitive. A day later, with a second examination of the discharge from the wound, St. Proteus, moderately sensitive to antibiotics.

 When using anolyte solution (ACC) in complex treatment, the patient's condition began to improve markedly, this was confirmed by clinical, biochemical analyzes. The color of the tissue of the wound surface changed from earthy to pink, islands of granulation appeared. On the fourth day after the replacement of traditional antiseptics with anolyte solution (ACC) during bacteriological examination of pathogenic microflora, which was separated from the wound, was not found. After the necrotomy, the wound was cleaned, covered with granulations. on the fifteenth day after the start of use of anolyte (ACC), a skin flap was plasticized and second-delayed sutures were applied to the skin.

 Example 2

Patient S., born in 1932, medical history 2908, was admitted to the department of ChLH GKB-11 on August 2, 1999. Clinical diagnosis: odontogenic phlegmon of the bottom of the oral cavity. Antibiotic Intolerance !!
Treatment: autopsy phlegmon, tooth extraction.

 An incision in the chin 18 cm long, revision and drainage of all cellular spaces of the bottom of the oral cavity. Locally, immediately after autopsy and hemostasis, the wound is washed with a 3% solution of hydrogen peroxide, then with anolyte solution (ACC). Perforated catheters for irrigation of the wound with anolyte solution (ACC) after 1.5-2 hours, 5-10 ml each, were inserted at the bottom of the wound from two strons. 100 ml oral baths per procedure were performed with anolyte solution (ACC) every 1.5-2 hours. The patient took orally a solution of catholyte (K), obtained on the STEL device, 100 ml three times a day. In a bacteriological study, after opening the phlegmon in the wound discharge, Staphylococcus aureus, moderately sensitive to antibiotics, was isolated. In an in vitro suspension study, after treatment of the isolated culture with anolyte solution (AKA), 100% bactericidal activity was obtained. On the third day after opening the phlegmon, no pathogenic microflora was detected in the discharge from the wound. The wound was actively granulating, the patient's condition was closer to satisfactory.

 On the tenth day after opening the phlegmon, a plastic skin flap was performed and second-delayed sutures were applied. The patient was treated without the use of antibiotics.

 Example 3

 Freestyle T., born 1977. Received on July 13, 1999.

 Clinical diagnosis: odontogenic phlegmon of the submandibular region and pterygo-mandibular space on the left.

 Treatment: opening the phlegmon with an external incision in the submandibular region on the left up to 10 cm long. In the complex treatment of phlegmon for local wound treatment and periodic wound irrigation, anolyte solution (ACC) was used. On the third day after opening the phlegmon in pathogenic flora separated from the wound was not found. For oral baths, the patient used anolyte solution (ACC). When using the anolyte solution on the second, especially on the third day, the swelling of the soft tissues of the face on the left decreased. On the ninth day after opening, a plastic flap was performed and secondary-delayed sutures were applied.

The neutral cathodic anolyte solution (ANC) was used for external treatment of purulent wounds of burn patients in the city clinical emergency hospital 1 (GKBSMP-1) in March 1999 and showed positive results, namely:
- ANC solution is sterile for 10 days from the date of its preparation;
- has a 100% bactericidal effect on gram-positive and gram-negative microorganisms in all exposures according to the time of sampling and various dilutions in one cubic centimeter;
- the simplicity of the method of preparation of the solution, environmental safety, low cost of preparing one liter of solution.

Literature
1. "Method for the prevention and treatment of initial dental caries" according to the description of the invention to the patent of the Russian Federation 2163114, IPC class A 61 K 6/00, 6/02, published in BI 5 02/20/2001

Claims (1)

A method of obtaining an aqueous solution of an antiseptic for local treatment of abscesses and phlegmon, including acidification of a salt solution during electrolysis with its direct current, characterized in that the aqueous solution of sodium chloride salt at a concentration of 0.0005-0.02% with the addition of potassium iodate to it in a concentration 0.0004-0.0006% in a mixture with running distilled water is subjected to electrochemical activation in an installation for the electrochemical synthesis of activated detergent disinfectant and sterilizing solutions of the STEL type, using a constant a current having a triple asymmetry of the current shape with a frequency of 10 to 50 Hz, and the installation time, current density and water pressure are set so as to obtain an acid cathodic anolyte at pH 2.3-2.5 or a neutral cathodic anolyte at pH 7 , 5-8.0, with a redox potential of 1100-1200 mV and a concentration of oxidizing agents With _ox = 25-150 mg / L.