JP2001170644A - Electrolytically produced cleaning water - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide cleaning water being electrolytically produced water having excellent sterilizing function and being suited to sterilize or wash an organism and skin. SOLUTION: This electrolytically produced water is used as the cleaning water for cleaning the organism and skin, and it is produced by electrolyzing diluted salt water as water to be electrolyzed, and the salt concentration of the electrolytically produced water is in a range of 0.60-0.90 wt.%, and its hemolytic property is very low and also a damage to a cell is low.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an electrolytically purified water used for cleaning or disinfecting a living body or skin.

[0002]

2. Description of the Related Art Electrolytic water is an excellent sterilizing water because it has a high bactericidal effect in acidic water (electrolytically generated acidic water) and neutral water, and is also used as alkaline water (electrolytically generated alkaline water). Since it has a high washing action, it is used in a wide range of fields as excellent washing water. For example, Japanese Unexamined Patent Publication No. Hei 7-118158 proposes to employ electrolytically generated acidic water as water for treating skin diseases of livestock.

[0003]

By the way, in the electrolytically produced water, it is considered that the living body, the surface of the skin, and the affected area of the skin are sterilized by utilizing the high sterilizing action of the electrolytically produced acidic water. Prior to these sterilizations, the surface of living bodies and skin,
It is conceivable to clean the affected area of skin damage. However, since the electrolyzed acidic water generally has hemolytic properties that destroy the red blood cells of animals, if there is damage to the skin, it will come into contact with the blood in the damaged area to dissolve the blood, There is a risk of damaging the cells (having cytotoxicity), and in this regard, the electrolytically generated acidic water is not necessarily suitable as sterilizing water for sterilizing living bodies and skin. . In addition, the electrolytically generated alkaline water may damage cells similarly to the electrolytically generated acidic water, and in this regard, the electrolytically generated alkaline water is not necessarily used as washing water for cleaning living bodies and skin. Not suitable.

Accordingly, an object of the present invention is to provide electrolytically produced water (electrolytically produced acidic water and electrolytically produced alkaline water) suitable as cleaning water for sterilizing and cleaning living bodies and skin. is there.

[0005]

SUMMARY OF THE INVENTION The present invention relates to an electrolytically purified water used for washing or sterilizing a living body or skin, wherein the electrolytically purified water is a dilute salt water used as an electrolyzed water. With a salt concentration of 0.60% by weight
０．９0.90% by weight.

The electrolytically produced purified water according to the present invention is produced by electrolyzing water to be electrolyzed having a salt concentration of 0.60% by weight to 0.90% by weight, or has a salt concentration of 0%.
The concentration of electrolyzed water generated by electrolyzing water to be electrolyzed in the range of 0.60% by weight to 0.60% by weight is adjusted to 0.60% by weight or more.
It has been adjusted to the range of 0.90% by weight.

[0007] The electrolytically produced purified water according to the present invention comprises:
Either acidic water produced in the anode compartment by diaphragm electrolysis and having a bactericidal action, or alkaline water produced in the cathode compartment by diaphragm electrolysis and having a washing action.

[0008] As the water to be electrolyzed for producing the electrolytically purified water according to the present invention, it is preferable to employ a saline solution.

[0009]

[Effects and Effects of the Invention] Electrolyzed water using dilute salt water as the electrolyzed water has an excellent bactericidal action in the case of electrolyzed acidic water, and the salt concentration is regulated in an appropriate range. In addition, the purified water has no or very low hemolysis, and has little damage to cells, and is suitable for cleaning living bodies and skin. In addition, electrolytically generated alkaline water has an excellent cleaning action, and by defining its salt concentration in an appropriate range, reduces damage to cells and is suitable for cleaning living bodies and skin. It becomes purified water.

The appropriate salinity of these purified waters is:
The electrolytically produced water (electrolytically produced acidic water, electrolytically produced alkaline water) having a salt concentration of 0.60% by weight to 0.90% by weight has a salt concentration of 0.60% by weight to 0.90% by weight.
Electrolysis of a diaphragm using 0% by weight of diluted salt water as the water to be electrolyzed, and the salt concentration is 0% to 0.60% by weight.
The electrolyzed water in the range of the electrolyzed acidic water generated by the diaphragm electrolysis, the salt concentration of the electrolyzed alkaline water is 0.6.
It is produced by adjusting the range of 0% to 0.90% by weight.

[0011]

EXAMPLES (Experiment 1) In this experiment, a hemolysis test was performed on human blood with electrolyzed acidic water, which is an example of electrolyzed purified water according to the present invention. , And a positive control were prepared as described below and performed based on the following test procedures. (Test solution) The test solution used in the present hemolytic experiment includes various types of dilute saline having different salt concentrations as electrolyzed water. Electrolyzed acidic water having various salt concentrations, and salt obtained by dissolving salt in distilled water. Various saline solutions having different salt concentrations with adjusted concentrations were employed.

The electrolyzed acidic water is electrolyzed in the anode compartment of the diaphragm electrolyzer and has a sterilizing effect. (Used blood) Blood of a healthy person was collected to prepare defibrinated blood, and the following items were confirmed and provided to a hemolytic experiment. That is, 0.2 ml of the prepared blood is added to 10 ml of physiological saline, centrifuged at 70 × g for 5 minutes to collect the supernatant, and the absorbance at 576 nm of the supernatant is measured. This ensures that the blood has not undergone hemolysis (absorbance 0.01
Below). In addition, a positive control (complete hemolysis) is required for the calculation of the hemolysis rate in the hemolysis experiment, and a solution in which 0.2 ml of defibrinated blood was added to 10 ml of distilled water to cause complete hemolysis was used as a positive control. . (Test operation) After adding defibrinated blood to the test solution 10 in the test tube at a ratio of 0.2, the test tube was sealed with a stopper and mixed by inverting only once, at 37 ° C for 1 hour. 2 hours and 4
After incubation for an hour, the mixture was centrifuged at 750 × g for 5 minutes to collect the supernatant. The absorbance at 540 nm or 576 nm of the maximum absorption of oxygenated hemoglobin in the separated supernatant was measured. Separately, the absorbance of the blank test solution and the positive control was measured, and the hemolysis rate was calculated based on the following equation (1). Table 1 below shows the relationship between the calculated hemolysis rate and the salt concentration.

[0013]

(Equation 1)

[0014]

[Table 1]

(Discussion) As is apparent from Table 1, the higher the salt concentration of each test solution, the lower the hemolysis, and the lower the salt concentration, the higher the hemolysis gradually. From the viewpoint of hemolysis, a test solution having a higher salt concentration is more suitable as washing water, but water having an excessive salt concentration is unsuitable as purified water because of its irritation. From this, the upper limit of the salt concentration is 0.90% by weight.
And the lower limit is 0.60% by weight.

Accordingly, the clean water having a bactericidal action and functioning as an excellent germicidal water has a salt concentration of 0.60% by weight or less.
An electrolytically produced acidic water in the range of 0.90% by weight,
The electrolyzed acidic water is suitable as cleansing water for sterilizing living bodies and skin. (Experiment 2) In this experiment, an experiment was performed to determine the degree of damage to the cells of the electrolytically produced acidic water, which is an example of the electrolytically produced purified water according to the present invention, and the electrolytically produced alkaline water. The test solution used for the experiment was prepared by using distilled water, a saline solution having a concentration of 0.9% by weight, tap water, distilled water, and a salt concentration of 0.1% by weight to 0.9% at pH 2.5.
% Of electrolyzed acidic water and electrolyzed alkaline water having a salt concentration of 0.1% to 0.9% by weight of sodium chloride at pH 11.5.

The cells used for the test are fibroblasts, and 5 ml of the test solution is injected into a petri dish from which a certain amount of fibroblasts have been collected, and incubated at 37 ° C. for 5 minutes. After washing to remove Hanks,
Further, 5 ml of the test solution was injected into the mixture, and the mixture was incubated at 37 ° C. for 5 minutes, and the resulting mixture was washed with 5 ml of Hanks to remove Hanks, and the cell viability was measured. The obtained results are shown in Tables 2 and 3 below.

[0018]

[Table 2]

[0019]

[Table 3]

(Discussion) As apparent from Tables 2 and 3, in the comparative experiment group using saline, tap water and distilled water as test solutions, the higher the salt concentration, the higher the cell survival rate. In addition, the higher the salt concentration of the electrolytically generated acidic water and the electrolytically generated alkaline water, the higher the cell survival rate. From the viewpoint of cell viability, a test solution having a higher salt concentration is more suitable as purified water, but water having an excessive salt concentration is unsuitable as purified water because of its irritation. From this, the upper limit of the salt concentration is 0.90% by weight, and the lower limit is 0.60% by weight.

Therefore, the electrolytically generated acidic water having a sterilizing action and functioning as excellent sterilizing water and the electrolytically generating alkaline water having a cleaning action and functioning as excellent cleaning water have a salt concentration of 0.60. When the content is in the range of from 0.9% by weight to 0.90% by weight, damage to cells is small, and thus it is suitable as purified water for sterilizing or washing a living body or skin.

Claims (5)

[Claims] Claims: 1. An electrolytically purified water used for washing or disinfecting a living body or skin, wherein the electrolytically purified water is electrolytically produced using dilute salt water as an electrolyzed water,
An electrolytically produced purified water having a salt concentration in a range of 0.60% by weight to 0.90% by weight. 2. The electro-generated cleaning water according to claim 1, which is produced by electrolyzing water to be electrolyzed having a salt concentration in a range of 0.60% by weight to 0.90% by weight. Characterized by electrolytically produced purified water. 3. The electrolytically produced purified water according to claim 1, wherein the salt concentration of the electrolytically produced water produced by electrolyzing the water to be electrolyzed having a salt concentration in the range of 0% by weight to 0.60% by weight. To 0.6
An electrolytically produced purified water characterized by being adjusted to a range of 0% by weight to 0.90% by weight. 4. The cleaning water produced by electrolysis according to claim 1, 2 or 3, which is an acidic water produced in an anode compartment by diaphragm electrolysis and has a bactericidal action. To produce electrolytically purified water. 5. The cleaning water produced by electrolysis according to claim 1, 2 or 3, which is an alkaline water produced in a cathode chamber by diaphragm electrolysis and has a cleaning action. To produce electrolytically purified water.