RU2422506C1 - Method to increase digestibility of nutrients in fodders for horses - Google Patents

Abstract

FIELD: veterinary science. ^ SUBSTANCE: method includes preparation of a fodder additive and addition of "Sakhabaktisubtil" preparation made of strains Bacillus subtilis TNP-3 and Bacillus subtilis TNP-5, in amount of 20 ml (100 billon CFU). The fodder additive containing the preparation "Sakhabaktisubtil" is added to the food ration 4 times every 15 days within 45 days to mares at the last months of pregnancy. ^ EFFECT: invention makes it possible to increase digestibility of fodder nutrients, to improve energy exchange and to increase final foal accretion. ^ 5 tbl

Description

The invention relates to agriculture, in particular to feeding horses of herd keeping.

There is a method of increasing the digestibility of feed and increasing the average daily weight gain of mares by introducing into the diet of the enzyme preparation celloviridin G 20 x and mineral additives (Buryakov N., Vasilieva Yu.Zh. Kombikorma, 2004, No. 2. - P. 56). However, the enzyme preparation contains only cellulase, which mainly decomposes fiber. To increase the digestibility of feed in the diet of pigs and birds add ready-made enzyme preparations (Kostamakhin NM Chief livestock specialist. 2006. No. 8. - S.20-22). However, the enzymes are mainly imported. Premixes with enzymes are produced by industrial technology in feed mills.

Known bacterial strain Bacillus subtilis TNP-3-DEPT used to disinfect litter and manure (RF Patent for the invention No. 2105810, publ. 27.02. 1998, bull. No. 6).

A method for the prevention of diarrheal diseases of newborn calves and piglets by using a suspension of bacterial strains of Bacillus subtilis TNP-3-DEP and Bacillus subtilis TNP-5-DEP (RF patent "Method for the prevention of diarrheal diseases of newborn calves and piglets", No. 22118924, A61P 1/100 , published on December 20, 2003, bull. No. 35). However, there is no information on the use of the drug "Sahabactisubtil", consisting of strains of bacteria Bacillus subtilis TNP-3-DEPT, and Bacillus subtilis TNP-5-DEPT as a microbial preparation with enzymatic activity to increase the digestibility of feed.

The basis of the invention is the task of developing a method of increasing the digestibility of feed of horses of herd content by using a feed additive containing a microbial preparation with enzymatic activity.

The technical result of the invention is a method of increasing the digestibility of feed of horses of herd content by introducing into the diet a feed additive with strains of microorganisms producing enzymes.

The bacterial strains of Bacillus subtilis TNP-3-DEP and Bacillus subtilis TNP-5-DEP are used, which have a complex of enzymatic activities (proteolytic, gelatinase, amylolytic, cellulolytic, β-gluconase, fructosyltransferase, xylanase pathogen), as well as and opportunistic microorganisms and the ability to normalize intestinal microbiocenosis, stimulate the body's immunobiological reactivity.

The technical result of the invention is achieved as follows: in the feed additive, consisting of gland flour, germinated wheat, beer pellets, Kempendiai salt, the preparation "Sahabactisubtil" is added and used in a single dose of 100 billion CFU every 15 days (4 times) for 45 days for mares. The composition of one dose of the feed additive: talic flour - 220 g, germinated wheat - 540 g, beer pellet - 210 g, Kempendiai salt - 30 g, Sahabactisubtil - 20 ml at the rate of 5 billion CFU / ml.

A feed supplement with the preparation "Sahabactisubtil" increases the digestibility of the nutrients of the diet and the efficiency of energy metabolism, as a result it helps to obtain a high business yield for foals.

The drug "Sahabactisubtil" is made from strains of bacteria Bacillus subtilis TNP-3-DEPT and Bacillus subtilis TNP-5-DEPT, deposited in the collection of microorganisms of the All-Russian State Institute of Control, Standardization and Certification of Veterinary Medicines (VGNKI). Certificates of deposit issued on October 17, 1994 for No. 902/9 and February 6, 2001 for 180/15 and 181/15). The bacterial strains of Bacillus subtilis TNP-3-DEPT and Bacillus subtilis TNP-5-DEPT are distinguished by the ability to produce enzymes and have antagonistic activity against pathogenic microorganisms.

Strains can be obtained in the laboratory for the development of microbial drugs GNU Yakutsk Research Institute of Agriculture RAAS and VGNKI.

The manufacture, control and testing of the drug "Sahabactisubtil" is carried out in accordance with the technical specifications TU 9384-003-00670203-06 and "Instructions for use of Sahabactisubtil for the prevention and treatment of dysbacteriosis of the gastrointestinal tract of newborn calves and pigs," approved. Russian Agricultural Supervision Ministry of Agriculture of the Russian Federation dated November 14, 2006

The bacterial strains of Bacillus subtilis TNP-3-DEPT and Bacillus subtilis TNP-5-DEP are grown in meat and peptone agar pH 7.0-7.2 for 5 days at 37 ° C, then suspended in different proportions in physiological saline solution of sodium chloride at a concentration 5 billion CFU / ml.

Take 20 ml of a suspension of the microbial preparation "Sahabactisubtil" containing 5 billion CFU / ml, and mix with a single dose of feed additive.

20 heads of foal mares were selected during the last months of foal. The experimental group (10 goals) was given a feed supplement with the microbial preparation Sahabactisubtil 20 ml (100 billion CFU) 4 times with an interval of 15 days. The control group (10 goals) receives the main diet, which consists of hay - 10 kg, oats - 2 kg, Kempendiai salt - 30 g.

In feed and feces, the initial moisture is determined by drying the samples at a temperature of 65 ° C in an oven.

The exchange energy of feed is determined by the regression equation given by the employees of the All-Union Research Institute of Horse Breeding (Kosharov A.N., Ugadchikov S.T., Memedeikin V.G., 1983).

The content of gross energy in the diets is calculated according to the equations developed by VIZH and VNIIIFBIP:

CE = 24.24 cP + 38.87 cJ + 18.39 cCl + 17.14 cBEV;

PE = 23.93pP + 32.66 pJ + 18.50 pCl + 17.00 pBEV;

OE = 19.46 pP + 35.43 pZh + 15.95 pCl + 15.95 pBEV, where cp, szh, sKl, sBEV are “raw” substances, g, pP, pZh, pCl, pBEV are digestible nutrients, d (Nadalyak E.A. et al. 1986; Grigoriev N.G., 1941; Shcheglov V.V., 1991).

The energy nutritional value of the feed is expressed in energy feed units (ECE) according to the formula: ECE _L = OE _L / 10, where OE _L is the amount of exchange energy of feed for horses, MJ (Petukhova E.A. et al., 1990).

Experiments on the digestibility of nutrients of feeds are carried out according to the generally accepted method of direct experiments (Ovsyannikov A.I., 1976) with accurate consideration of the amount of feed and feces eaten. Based on the weight and chemical composition of the given feed, its residues and feces, the amount of nutrients consumed with the feed and excreted in the feces is determined. The difference determines the amount of digested substances.

The ratio of the digested portion to the total amount of nutrients consumed with the feed, expressed as a percentage, is the coefficient of the apparent digestibility of the feed nutrients. In order to study the effect of the drug "Sahabaktisubtil" with a feed additive on the digestibility of nutrients in diets, a physiological experiment is carried out on 6 mares (three mares from each group). Duration of experience: preparatory period - 4 days, accounting 6 days. Sampling of feed and scraps for analysis is carried out according to the generally accepted method daily for each animal. Selected samples are immediately weighed and dried in ovens.

The content of dry matter, crude protein, digestible protein, crude fat, crude fiber, BeV, macro - and microelements and vitamins in the diet within the standards for feeding mares (table 1).

Table 1 Diet for feeding mares of the Yakut breed (per 1 head per day) Indicator control experienced Consumed by: Hay, g 8570 8530 Oats, g 1710 860 Feed supplement - 900 The diet contained: exchange energy, MJ 96.36 102.66 dry matter, g 10280 10290 crude protein, g 1000 1030 digestible protein, g 700 730 crude fat, g 180 180 crude fiber, g 3430 3420 Bev, g 4290 4390 calcium g 107.45 109.46 phosphorus, g 18.54 22.11 magnesium g 89.0 88.87 copper mg 71 79.29 zinc mg 269.0 304.0 cobalt, mg 5,0 7.71 manganese, mg 260,0 309.43 iodine, mg 3.25 4.15 carotene, mg - 1.05 vitamin E mg 675.4 716.09 B ₁ mg 25.0 31.6 B ₂ mg 128.0 130.0 B ₃ mg 250,5 261.71 ₄ mg 846 843.0

The introduction of the drug "Sahabactisubtil" with a feed additive into the diet of an experimental group of animals increases the consumption of dry matter by 10 g, or 0.09%, organic matter by 120 g, or 1.35%, and BEV by 100 g more, or 2.33% (table 2).

table 2 The amount of nutrients consumed by the Yakut breed mares, g per head per day Indicator Group control experienced Dry matter 10280 10290 Organic matter 8900 9020 Crude protein 1000 1030 Crude fiber 3430 3420 Crude fat 180 180 Bev 4290 4390

Based on the data on the amount of consumed and excreted nutrients, the amount of digested nutrients is calculated (table 3).

Table 3 The average daily amount of nutrients digested by mares of the Yakut breed, g per head per day Indicator Group control experienced Dry matter 6439 ± 0.02 6970 ± 0.46 Organic matter 5839 6130 Crude protein 700 ± 0.01 730 ± 0.02 Crude fiber 1800 ± 0.01 1930 ± 0.23 Crude fat 110 ± 0.005 120 ± 0.003 Bev 3230 ± 0.005 3350 ± 0.07

The data obtained indicate that the animals of the experimental group digest more dry matter by 531 g (8.25%), organic matter by 291 g (4.98%), crude protein by 30 g (4.28%), crude fiber - 130 g (7.22%), crude fat - 10 g (9.09%) and BEV - 120 g (3.71%).

An important indicator of animal use of nutrients in consumed feed is digestibility ratios, which are the ratio of digested nutrients to consumed, expressed as a percentage (table 4).

Table 4 The digestibility rate of nutrients in the mares of the Yakut breed,% Indicator Group control experienced Dry matter 62.64 ± 0.19 * 67.73 ± 4.46 * Organic matter 65.61 67.96 Crude protein 70.00 ± 0.5 ** 70.87 ± 1.79 ** Crude fiber 52.48 ± 0.29 56.43 ± 6.87 Crude fat 61.11 ± 3.0 66.66 ± 1.74 Bev 75.29 ± 0.2 76.31 ± 1.60 * (P <0.95); ** (P> 0.99).

A comparative analysis of digestibility coefficients shows that in the mares of the experimental groups, the digestibility of dry matter is 8.12% (P <0.95), organic matter - 3.58, raw protein - 1.24 (P> 0.99) , crude fiber - by 7.53, crude fat - by 9.08% and BEV - by 1.20%.

Therefore, the animals of the experimental group, receiving the drug "Sahabactisubtil" with a feed additive based on local raw materials, better digest nutrients compared to analogues of the control group.

Changes in the body can also be judged by the balance of energy (table 5).

Table 5 Distribution and use of energy in mares of the Yakut breed, on average per day, MJ Indicator Group control experienced RE accepted with feed 167.86 170.11 Allocated energy with feces 61.62 56.07 % of renewable energy 36.71 32.96 Overcooked energy 106.24 114.04 % of EV 63.29 67.04 Urine and Methane Energy 9.88 11.38 % of PE 9.30 9.98 Exchange energy 96.36 102.66 % of PE 90.70 90.02 % of renewable energy 57.4 60.35

The consumption of gross energy by experimental foals of both groups is practically the same.

The obtained energy of the mare of the experimental group is maximally digested: the digestible energy index of them is 114.04 MJ per day, which is 7.80 MJ / day or 7.34% more than that of the mares of the control group.

The level of exchange energy in the experimental group is 102.66 MJ. The same indicator in animals of the control group is less by 6.30 MJ, or by 6.54%.

Consequently, feeding Sagabactisubtil with a feed additive to foal mares improves energy use and energy exchange efficiency.

The inclusion of the drug "Sahabactisubtil" with a feed additive in the hay-oat diet of the mares during stationary feeding had a positive effect on the business yield of the foals compared with the control group of animals. The business yield of foals in the experimental group was 70%, and in the control - 60%.

Thus, the introduction of a fodder supplement with the Sahabactisubtil preparation into the diet of mares increases the digestibility of the main nutrients of the feed, enhances energy metabolism and helps increase the business yield of foals.

Claims (1)

A method of increasing the digestibility of nutrients of feed for horses by adding a feed additive with an enzyme preparation to the diet, characterized in that the preparation “Sahabactisubtil” from the bacterial strains Bacillus subtilis TNP-3-DEPT and Bacillus subtilis TNP-5-DEPT is used as an enzyme preparation which is added to the feed supplement at a dose of 20 ml (100 billion CFU) every 15 days (4 times) for 45 days to mares of the last months of foal.