RU2402343C1 - Method for preparing mistletoe tincture - Google Patents

Abstract

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmacy. An aqueous-alcoholic tincture exhibiting hypolipidemic and hypotensive activity is prepared by infusion and filtration. The raw materials are mistletoe shoot, leaves, berries collected in autumn in the ratio 1:1:1 extracted in 60% alcohol in the ratio of raw materials to extractant 1:2 for 4 days followed by triple heating to 60°C and cooling, filtration to prepare the first fraction, the oil cake is soaked once again in 90% alcohol in the ratio 1:2, infused for 10 days to produce the second fraction, then the dried mistletoe leaves collected in January are placed in distilled water in the ratio of raw materials to extractant 1:10 with 10% ascorbic acid added, kept for 24 o'clock, filtered to produce aqueous tincture, the fractions are mixed together and added with aqueous mistletoe tincture to alcohol concentration 60%.

EFFECT: invention allows improving hypolipidemic and hypotensive activity of the product.

1 ex

Description

The invention relates to pharmacy and relates to a method for producing funds from plant materials with hypolipidemic, antihypertensive activity.

A known method of obtaining an extract from white mistletoe leaves by soaking them with water in a ratio of 1: 2 with a temperature of 95-100 ° C and insisting for 40-60 hours, after which they fill in with a 50% aqueous-alcohol mixture in a ratio of 1: 5 and insist at periodic stirring for 25-30 days at a temperature of 28-35 ° C. Then the vegetable raw materials are squeezed, the pressed vegetable raw materials are poured with tap water in a ratio of 1:10 and boiled for 25-35 minutes, insisted for 8-10 hours, the broth is separated from the raw materials, filtered and mixed with the extract (USSR Author's Certificate No. 940730).

This method of extracting takes a fairly long time.

A known method of obtaining tincture of white mistletoe from dried raw materials for 4 Homeopathic Pharmacopoeia Schwab in a ratio of 1:10.

(Homeopathic medicines, a guide to the description and manufacture, Wilmar Schwabe, edited by V.I. Rybak, Moscow, 1967).

1. Dried fruits and leaves of white mistletoe grind.

2. Tincture is prepared at a ratio of 1:10 by percolation, for this 1 kg of white mistletoe fruits and leaves is poured with 5 times the amount of extractant (5 L) at a 50% concentration - ethyl alcohol and left to infuse for 24-48 hours, then the extractant is drained completely and measure the volume of extraction. The raw material is extracted twice more with fresh extractant so that the combined extracts are 10 liters. Each stage of infusion lasts 24-48 hours.

3. The combined extracts are settled at a temperature of 10 ° C for two days and filtered.

It turns out tincture 1:10.

A known method of obtaining a water-alcohol tincture with hypolipidemic and antihypertensive activity (RU 2171113 C1 07.27.2001)

This method of obtaining tinctures is taken by us as a prototype. The technical task of the invention is to increase the pharmacological action of tincture of mistletoe white.

The technical problem is solved by the proposed method.

For the manufacture of tinctures, raw white mistletoe, a parasitic dioecious shrub growing on a pear fruit tree are used. The raw materials are young twigs, leaves and berries. Raw materials collected in the fall, in a ratio of 1: 1: 1, are soaked with 60% alcohol at a ratio of raw materials-extractant 1: 2, insisted in a dark place for 4 days, then heated three times to 60 ° C, and after each heating, there is rapid cooling.

Then the obtained extract is filtered and the first fraction is obtained.

The cake is weighed and re-filled with 90% alcohol at a ratio of 1: 2 and insisted for 10 days. Then the extraction is filtered and a second fraction is obtained.

The dried leaves of the white mistletoe, collected in the month of January, are crushed and poured with freshly prepared distilled water in the ratio of raw material-extractant 1:10, and freshly prepared 10% solution of ascorbic acid is added to the solution. Leave for 24 hours, filter through a filter.

The first two fractions are mixed with each other and add water mistletoe infusion to an alcohol concentration of 60%

An example of a specific implementation.

100 g of young twigs, 100 g of leaves and 100 g of white mistletoe berries collected in the fall are mixed with each other, pour 600 ml of 60% alcohol and infused for 4 days, then heated for the first time to 60 ° C, then cooled and repeated twice.

The resulting extract is filtered and the first fraction is obtained.

Cake is poured into 600 ml of 90% alcohol and insisted for 10 days.

The resulting extract is filtered.

100 g of dry leaves collected in January, pour 1000 ml with distilled water, 10% ascorbic acid solution is added to the solution, insist 24 hours.

The fractions are mixed with each other and adjusted by aqueous extraction to an alcohol concentration of 60%.

White mistletoe, twigs and leaves, contains viscotoxin consisting of amino acids and sugars, viscerol, alpha-viscol, beta-viscol, oleanolic, ursolic acid, alkaloid-like substance C ₈ H ₁₁ N, choline, acetylcholine, propionylcholine, amines, tyramine, alcohols , fatty oil, carotene, resins, vitamin C, resinous substances (up to 9.73%) (Zavrazhnov V.I., Kitaeva R.I., Khmelev KF Medicinal plants: Therapeutic and preventive use. Voronezh: Publishing House Voronezh State University, 1993 .-- 480 p. (431-434 p.).

On the basis of the Verbena homeopathic laboratory, tests were conducted to determine the pharmacological activity of the obtained tincture according to the proposed method and the prototype method.

To determine the lipid-lowering activity, outbred rats weighing 180.0-200.0 g were taken, which were injected intragastrically (through a probe) with tincture of mistletoe white (control animals were given tincture obtained by the prototype method). After 2-day administration of doses of tincture with a volume of 5 mg / kg, the animals of the experimental and control groups were sacrificed and their blood was taken to determine the level of cholesterol.

The results obtained showed that the proposed tincture of the drug in doses of 5 mg / kg reduced the concentration of cholesterol in rat blood serum by 18% relative to the prototype.

The test for antihypertensive activity was carried out on cats, as a comparison preparations used homeopathic tincture obtained by the proposed method and the method of the prototype. It was found that tincture of mistletoe at a dose of 50 mg / kg 2 minutes after administration reduces blood pressure by 29.0 and 23.3%, and after 5 minutes by 18.0 and 14.7%, respectively.

Thus, the proposed method for obtaining a homeopathic unstable mistletoe white allows you to get a tincture with increased lipid-lowering and hypotensive activity and use it in homeopathy for the manufacture of homeopathic medicines.

Claims (1)

A method of obtaining a water-alcohol tincture with hypolipidemic and hypotensive activity, the method of insisting and filtering, characterized in that the raw materials are young twigs, leaves, white mistletoe berries collected in autumn, in a ratio of 1: 1: 1, extraction is carried out with 60% alcohol with the ratio of raw materials-extractant 1: 2 for 4 days, three times heating to 60 ° C and cooling, filtering, get the first fraction, cake is re-soaked with 90% alcohol at a ratio of 1: 2, insist for 10 days and get the second fraction, then dried up The white mistletoe leaves, collected in the month of January, are poured with distilled water in a feed-extract ratio of 1:10, a 10% solution of ascorbic acid is added, left for 24 hours, filtered, an aqueous infusion is obtained, the fractions are mixed together and an aqueous infusion is added mistletoe to an alcohol concentration of 60%.