RU2371175C2 - Method of work efficiency level increase for laboratory animals in experiment - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention concerns experimental pharmacology. Method involves administration of 6-diisopropylaminomethyl-2,3-dihydroquercetin C₂₂H₂₇NO₇ to laboratory animals.

EFFECT: enhanced work efficiency in Porsalt test.

1 tbl, 1 ex

Description

The invention relates to medicine, namely to experimental pharmacology.

It is known the use in experiment and medical practice of a preparation of bemitil, which has an actoprotective effect [1]. Today, this connection is obsolete.

The purpose of the invention is the expansion of the arsenal of tools to improve performance in laboratory animals in the experiment.

This goal is achieved by the use of NK-2 (6-diisopropylaminomethyl-2,3-dihydroquercetin, C ₂₂ H ₂₇ NO ₇ ) as a tool with the properties of an actoprotector - a tool that improves performance.

In experiments on white non-inbred male rats weighing 180-220 g, the effect of NK-2 (6-diisopropylaminomethyl-2,3-dihydroquercetin, C ₂₂ H ₂₇ NO ₇ ) was studied to increase the level of performance in an artificial instrumental setting: in the Porsolt test [2 ].

NK-2 was administered as a solution intraperitoneally at a dose of 20 mg / kg of animal weight in the morning hours 40 minutes before the experiment for five days of testing.

The control group was animals that received saline in the same volume.

When using NK-2 (6-diisopropylaminomethyl-2,3-dihydroquercetin, C ₂₂ H ₂₇ NO ₇ ) in experimental animals tested in the Porsolt test, the following changes in the registered health parameter were revealed in comparison with the control group. Table 1 presents the average values for all days of testing.

The immobility rate in the experimental group receiving NK-2 significantly (p <0.05) decreases compared to the control group. Thus, NK-2 improves performance in the Porsolt test, which indicates the presence of an actoprotective effect in the test substance.

Example

An experiment was conducted to study changes in performance in rats treated with NK-2 at a dose of 20 mg / kg in the Porsolt test. The value of the immobility index in the experimental group, which was administered the test compound, significantly (p <0.05) decreased 1.32 times compared with the control group (table 1). Thus, NK-2 improves performance in the Porsolt test, which indicates the presence of an actoprotective effect in the studied drug.

The used compound 6-diisopropylaminomethyl-2,3-dihydroquercetin, C ₂₂ H ₂₇ NO ₇ is new, and therefore there are no bibliographic data on its synthesis. Based on this, we give the structural formula of 6-diisopropylaminomethyl-2,3-dihydroquercetin, proven by known methods, we give physicochemical constants with a description of the method of preparation.

SYNTHESIS INFORMATION

chemical compound

6-diisopropylaminomethyl-2,3-dihydroquercetin, C ₂₂ H ₂₇ NO ₇

This compound was synthesized in the scientific laboratory of the Department of Organic Chemistry, Department of Chemistry, Moscow State Pedagogical University.

The structure of the compound was proved by NMR spectroscopy on ¹ H and ¹³ C nuclei. It was confirmed by elemental analysis. Characterized by melting point.

Synthesis (production method): A mixture of 0.49 g (16.0 mmol) of formaldehyde (as paraform) and 1.62 g (16.0 mmol) of diisopropylaminomethyl in 40 ml of ethanol was stirred, while heating to 70 ° C, until complete homogenization, boiled for 30 minutes, cooled. The resulting solution was added with stirring to a solution of 5.58 g (16.0 mmol) of 2,3-dihydroquercetin dihydrate in 80 ml of ethanol, stirred for 2 hours at 20 ° C. The precipitate was filtered off, washed with ethanol (2 × 30 ml), ether (2 × 30 ml) and dried in vacuum to constant weight. Yield 3.60 g (54%).

Physico-chemical characteristics: so pl. 245-248 ° C, ¹ H NMR spectrum, b, ppm (J, Hz): 1.15 dd

(12 CH ₃ -CH), 3.24 m (2H, CH-CH ₃ ), 3.86 s (2H, CH ₂ ), 4, 42d (1H, C ³ , J (HH) 11.00), 4, 78 d (1H, C ₂ H, J (HH) 11.00), 5.28 s (1H, C ₇ OH), 5.43 s (1H, C ₈ H), 6.75 s (24, C ₅ ' ₆ 'H), 6.91 s (1H, C ₂ ' H), 8.04 s (1H, C ₄ 'OH), 8.19 s (1H, C ₃ ' OH), 11.15 s (1H, C ₃ OH), 12.10 s (1H, C ₅ OH). NMR ¹³ C, b, ppm: 18.85 ( CH ₃ -CH), 20.54 ( CH ₃ -CH), 42.15 (N-CH ₂ -C ₅ ), 46.25 (CH2- N- CH ), 50.13 (CH ₂ -N- CH ), 71.84 (C ³ ), 83.31 (C ² ), 97.04 (C ₈ ), 98.02 (C ₆ ), 99 47 (C ¹⁰ ), 115.58 (C ⁵ '), 115.78 (C ² '), 119.80 (C ₆ '), 128.95 (C ¹ '), 145.49 (C ³ ' ), 146.22 (C ₄ '), 160.89 (C ₉ ), 162.10 (C ₅ ), 175.68 (C ₇ ), 195.89 (C ₄ ).

Found,%: C 63.28; H 6.50; N, 3.33. C ₂₂ H ₂₇ NO ₇ . Calculated,%; C 63.31; H 6.47; N, 3.36.

Table 1 Groups Immobility Time (sec) Control 51.64 ± 8.13 SNK-2 39.21 ± 4.88 *

Information sources

1. Actoprotectors. http://doping.net.ru/bulanovl8.htm

2. Porsolt R. D., McArthur R. A., Lenegre A. Psychotropic screening procedures: In: Methods in Behavioral Pharmacology, Ed. F. van Haaren, Elsevier, New York, 1993, P.23-51.

Claims (1)

A method of increasing the working capacity of laboratory animals in an experiment, including the use of a chemical compound, characterized in that 6-diisopropylaminomethyl-2,3-dihydroquercetin, C ₂₂ H ₂₇ NO ₇ , is introduced.