RU2340215C2 - DIETARY SUPPLEMENT BASED ON CYSTS OF Artemia salina BRINE SHRIMPS AND PRODUCT FOR INTERNAL OR EXTERNAL USE BASED ON IT - Google Patents

Abstract

FIELD: food industry.

SUBSTANCE: dietary supplement is a lipid-vitamin complex produced from the extract of crushed Artemia salina brine shrimp cysts. Acid number of the dietary supplement amounts to 69, iodine number - 44.7, peroxide number - less than 0.002, relation of polyunsaturated fat acids to essential fatty acids - 4:1; the dietary supplement contains polyunsaturated fatty acids, vitamins, sterols, squalene, growth factors and other liposoluble biologically active compounds in the specified ratio. The dietary supplement is introduced into the product for internal or external use in the amount of 0.01-30.0 wt %..

EFFECT: invention allows manufacturing of a product with increased biological activity and decreased content of allergenic substances.

5 cl, 13 ex

Description

The invention relates to food, pharmaceutical or cosmetic dietary supplements and can be used in the food industry, cosmetics and medicine.

Despite the achievements of modern science, the problem of the deficiency of complex additives balanced in vitamins, polyunsaturated fatty acids (PUFAs) and amino acids remains relevant. The value of cysts or individuals of Artemia is determined by its chemical composition, which is characterized by a sufficiently high content of proteins, fats, essential amino acids and fatty acids, vitamins, sterols, carotenoids and other biologically active compounds. Artemia is common throughout the globe. In Russia, it lives in salt water bodies of the coast of the southern seas, lakes of the Caucasus, Kyrgyzstan, Western and Eastern Siberia. The water area of the studied reservoirs of Western Siberia alone is 2.1 thousand km ² , of which 1.1 thousand km ² in the Altai Territory.

It is known to use whole eggs of the gill leg of Artemia salmon (Artemia salina (L) as a dry powder as a feed additive for birds, as well as a feed additive, which use whole eggs of the gill leg of Artemia salmon (Artemia (L) in the form of dry powder (up to 10-15 wt.%) With the addition of grain or other feed (up to 100 wt.%) Used for feeding laying hens (RF Patent No. 2158519, IPC A23K 1/16, 1/00; publ. 10.11.2000, )

However, the whole Artemia eggs are covered with a chitinous membrane and can be absorbed only by the body of birds, which grind this product in the corresponding section of the digestive tract. Using whole Artemia eggs as a dietary supplement in a person’s diet or as a component of a medicinal, cosmetic or balneotherapy drug is inefficient due to the poor digestibility of whole Artemia cysts in this form.

Known food dietary supplement, which is a purified concentrate of omega-3 polyunsaturated fatty acids, obtained from the extract of crustaceans Artemia salina (US Patent No. 5340594, IPC A23D 9/00, publ. 23.08.1994).

The disadvantage of this biologically active additive is the absence of many useful components in it that are contained in the lipid-vitamin complex of Artemia cysts (polyunsaturated fatty acids omega-6, vitamins, squalene and others), which limits its biological activity and scope.

A dry form of a dietary supplement based on Artemia salina cyst is known, which is crushed to an ultrafine state with a particle size of not more than 20 μm and a moisture content of not more than 5.0 wt.%. The dry form of the treatment-and-prophylactic preparation is a tablet made in the form of a monolithic disk-shaped solid body from Artemia cyst powder, or a cylindrical hollow detachable capsule filled with Artemia cyst powder, or a gas-tight bag filled with Artemia cyst powder in vacuum (Certificate of Useful RF model No. 45270, IPC A61K 35/56, publ. 10.05.2005).

Known use in the composition of solid rennet cheese food biological active additives based on decapsulated cysts of brine shrimp Artemia salina in an amount of 0.25-1.0 wt.% (Patent application of the Russian Federation No. 2003112517, IPC A23C 19/068, publ. 27.11.2004 )

The closest analogue (prototype) is also known, which is a biologically active additive for medical and cosmetic preparations, which has an antiproliferative effect and is an aqueous or alcoholic extract of eggs (cysts) of crustaceans Artemia salina (US Patent No. 6342254, IPC АКК 35/78, 35 / 12, published on January 29, 2002). The tool is proposed to be used in medicine and cosmetics for the treatment and prevention of skin diseases.

The closest analogue (prototype) of known products is the composition of creams, ointments, emulsions based on the extract of crustaceans Artemia salina, used in cosmetics for the prevention and treatment of skin and hair (French Patent No. 2817748, IPC A61K 35/56, 7/48, publ. June 14, 2002).

The disadvantage of the above analogues, including the prototype, is that the liquid extract of eggs (cysts) or adult crustaceans Artemia salina contains, in addition to the lipid-vitamin complex, a large number of proteins, peptides and amino acids, which when used in medicine, the food industry or cosmetics can cause allergic reactions of the body, which affect 20 to 40% of children and up to 15% of the adult population of Russia and other countries. For example, an amino acid such as arginine is not recommended for people suffering from a chronic herpes virus disease. Arginine enhances the recurrence of this disease. The above factors narrow the scope of these highly allergenic dietary supplements in the food industry, as well as in medicine and cosmetics.

The technical result of the invention is the creation of a dietary supplement based on Artemia salmon cysts for external or internal use with a reduced content of allergenic substances, as well as products based on it.

The indicated technical result is achieved by the creation of a biologically active supplement, which is a lipid-vitamin complex from the extract of crushed cysts of Artemia salina for external or internal use.

The lipid-vitamin complex has an acid number of 69, an iodine number of 44.7, a peroxide value of less than 0.002%, the ratio of polyunsaturated fatty acids to essential fatty acids 4: 1 and contains the following composition of components (wt.%):

vitamin A (retinol) 0.35 vitamin E (tocopherol) 0.2 cholesterol 3.2 squalene 2.0 myristic acid (C14: 0) 0.6 pamit oleic acid (C16: 1) 3,5 palmitic acid (C16: 0) 6.0 margaric acid (C17: 0) 11.0 stearic acid (C18: 0) 4.0 oleic acid (C18: 1) 6.0 Vitamin F: linoleic acid (C18: 2) 6.5 alpha linolenic acid (C18: 3) 45.0 gamma linolenic acid (C18: 3) 7.0 arachidonic acid (C20: 4) 0.8 eicosaenoic acid (C20: 1) 0.3 eicosatrienic acid (C20: 3) 0.4 eicosapentaenoic acid (C20: 5) 2,3 docosahexaenoic acid (C22: 6) 0.5 other fatty acids
sterols, carotenoids,
cholesterol derivatives the rest is up to 100%

The specified technical result is also achieved by the fact that the product for external or internal use, comprising a biologically active additive and a filler, according to the invention as a biologically active additive contains a lipid-vitamin fraction of the extract of crushed cysts of Artemia salina with the following content of components (wt.%):

Lipid-vitamin complex

from extract crushed crustacean cysts Artemia salina 0.01-30.0 Filler for outdoor or domestic use the rest is up to 100%

As a filler for internal use, it contains sugar, or vegetable oil, or salt, or chalk and starch in a ratio of 2: 1. As a filler for external use, it contains a gel base. The above composition of the lipid – vitamin complex from cysts extract Artemia salina confirms the high biological activity of this drug.

Thus, the lipid – vitamin complex from cysts extract Artemia salina has a minimum content of proteins, peptides, and amino acids, which ensures low allergenicity to this product.

Example 1. Carbon dioxide technology for producing a lipid-vitamin complex from Artemia cyst extract

The eggs (cysts) of crustaceans Artemia salina are obtained from lakes in Western Siberia, repeatedly washed in fresh water from impurities, salts and low-quality eggs. At the stage of washing, the whole Artemia crustacean cysts can be additionally treated with detergents. The wet product is dried in an air stream with a moisture content of not more than 5 wt.%, At a temperature not exceeding 50 ° C. Cysts are ground in a vortex mill, for example, developed by the company Vortex Technologies, Novosibirsk (Russian Federation patent No. 2057588, MKI V02C 19/06, publ. 04/10/96), which is a gas-dynamic mill using compressed gas energy for accelerating the crushed particles of the product in a vortex flow to an ultrafine state with a particle size of not more than 20 microns and a moisture content of not more than 5.0 wt.%. As a grinder can also serve as a ball or disk mill or other technical means that provide the appropriate dispersed composition of the product without compromising its useful properties.

Next, the powder (10.0 kg) is loaded into the extractor and extracted with liquefied carbon dioxide for 2 hours at a temperature of 26 ° C and a pressure of 7.0 MPa with the release of carbon dioxide extract. The product yield is 300 g. Carbon dioxide extract is obtained in the form of a thick, oily liquid of dark brown color with a specific odor. The main physical and chemical parameters at a temperature of 23-27 ° C: density 855.3 kg / m ³ ; refractive index 1.4706; specific gravity 0.9965; % ash - 2.4 / 3.4; acid number 69; iodine number 44.7; the peroxide value is less than 0.002%, the ratio of polyunsaturated fatty acids (PUFAs) to essential fatty acids (EFAs) is 4: 1.

The determination of fatty acids and fat-soluble vitamins in the resulting lipid-vitamin complex was carried out by high performance liquid chromatography. The lipid-vitamin complex includes the following component composition (wt.%):

vitamin A (retinol) 0.35 vitamin E (tocopherol) 0.2 cholesterol 3.2 squalene 2.0 organic acids: myristic (C14: 0) 0.6 pamit oleic (C16: 1) 3,5 palmitic (C16: 0) 6.0 margarine (C17: 0) 11.0 stearic (C18: 0) 4.0 oleic (C18: 1) 6.0 Vitamin F: linoleic (C18: 2) 6.5 alpha-linolenic (C18: 3) 45.0 gamma-linolenic (C18: 3) 7.0 arachidonic (C20: 4) 0.8 eicosaenoic (C20: 1) 0.3 eicosatriene (C20: 3) 0.4 eicosapentaenoic (C20: 5) 2,3 docosahexaenoic (C22: 6) 0.5 other fatty acids, sterols, arotinoids, derivatives
cholesterol the rest is up to 100%

Of particular importance are fatty acids, which are part of lipids and determine their properties. The most valuable components are vitamins A, E, F, cholesterol, squalene, PUFA, most of which are free, essential PUFAs are linoleic, linolenic (including gamma-linolenic), arachidonic. The ratio of PUFA omega-3: omega-6 = 1: 2.

Thus, the composition and ratio of lipids, and, above all, fatty acids, characterize the resulting complex as a valuable biological product.

Further, the resulting product can be used as a dietary supplement or component of a cosmetic product.

A food or pharmaceutical product in dry form is prepared in a powder mixer. The mixer is a container with a spherical bottom having an axis of rotation inclined to the horizontal. The mixing efficiency is ensured by inert bodies of a spherical shape, significantly smaller than the bottom of the tank of radius of curvature, and significantly larger specific gravity than the specific gravity of the mixed components of the material. Inert bodies prevent aggregation of particles and provide mixing with particles of any dispersion in a small gap between the surface of inert bodies and the bottom of the tank. The number of inert bodies ensures their location in only one layer to avoid their active collision and, as a result, to avoid overheating of the mixed materials.

Obtaining a pharmaceutical preparation

The components of the pharmaceutical preparation are introduced into the mixer in accordance with Examples 2-4. The mixture is then passed through a tablet press. The following are examples of 2-4 pharmaceutical preparations.

Example 2, wt.%:

lipid-vitamin complex from extract of crushed cysts crustacean Artemia salina 1,0 a mixture of chalk with starch (2: 1) the rest is up to 100%

Example 3, wt.%:

lipid-vitamin complex from extract of crushed cysts crustacean Artemia salina 5,0 a mixture of chalk with starch (2: 1) the rest is up to 100%

Example 4, wt.%:

lipid-vitamin complex from extract of crushed cysts crustacean Artemia salina 10.0 a mixture of chalk with starch (2: 1) the rest is up to 100%

Getting a food supplement

The components of the food product are introduced into the mixer in accordance with Examples 5-7. Next, the resulting product is Packed.

Example 5, wt.%:

lipid-vitamin complex from extract of crushed cysts crustacean Artemia salina 0.1 sugar the rest is up to 100%

Example 6, wt.%:

lipid-vitamin complex from extract of crushed cysts crustacean Artemia salina 0.5 sugar the rest is up to 100%

Next, the resulting product is Packed.

Example 7, wt.%:

lipid-vitamin complex from extract of crushed cysts crustacean Artemia salina 1,0 salt the rest is up to 100%

Obtaining a food product in liquid form

The components of the food product are introduced into the homogenizer in accordance with Examples 8-10. Next, the resulting product is poured into containers.

Example 8, wt.%:

Lipid Vitamin Fraction crushed cyst extract crustacean Artemia salina 0.01 vegetable oil the rest is up to 100%

Example 9, wt.%:

Lipid Vitamin Fraction crushed cyst extract crustacean Artemia salina 0.25 vegetable oil the rest is up to 100%

Example 10, wt.%:

Lipid Vitamin Fraction crushed cyst extract crustacean Artemia salina 0.5 vegetable oil the rest is up to 100%

Getting a cosmetic

In the manufacture of the drug, simple technological equipment is used, equipped with a reactor with a stirrer.

To prepare the cream, a gel base is loaded into a stirred reactor at a temperature of + (18-20) ° C and a dietary supplement is added with stirring according to the following formulation (examples 11-13, wt.%).

The components are mixed for 20-30 minutes and the pH of the mixture is measured. If the pH of the mixture is below 7.2 (the gel has an acidic environment), then a phosphate-salt additive (Na ₂ HPO ₄ × 12H ₂ O) is added to the composition in an amount sufficient to maintain a pH of 7.2. Then the mixture is homogenized again in the reactor for 20-30 minutes, the finished cosmetic product is packaged in a container and stored at a temperature of + 7 ° C. The cold method of preparing the cream allows you to maximize the activity of its constituents.

Example 11, wt.%:

lipid-vitamin fraction crushed cyst extract crustacean Artemia salina 5,0 gel base the rest is up to 100%

Example 12, wt.%:

lipid-vitamin fraction crushed cyst extract crustacean Artemia salina 17.0 gel base the rest is up to 100%

Example 13, wt.%:

lipid-vitamin fraction crushed cyst extract crustacean Artemia salina 30,0 gel base the rest is up to 100%

Thus, the obtained lipid – vitamin complex from extract of Artemia salina cysts has a minimal content of proteins, peptides, and amino acids, which ensures low allergenicity to this product.

Claims (5)

1. A dietary supplement based on cysts of Artemia salina, characterized in that it is a lipid-vitamin complex from the extract of crushed cysts of Artemia salina, has an acid number of 69, an iodine number of 44.7, a peroxide value of less than 0.002%, the ratio of polyunsaturated fatty acids to essential fatty acids 4: 1 and includes the following composition of components, wt.%: vitamin A (retinol) 0.35 vitamin E (tocopherol) 0.2 cholesterol 3.2 squalene 2.0 myristic acid (C14: 0) 0.6 pamit oleic acid (C16: 1) 3,5 palmitic acid (C16: 0) 6.0 margaric acid (C 17: 0) 11.0 stearic acid (C 18: 0) 4.0 oleic acid (C18: 1) 6.0 Vitamin F: linoleic acid (C18: 2) 6.5 alpha linolenic acid (C18: 3) 45.0 gamma linolenic acid (C18: 3) 7.0 arachidonic acid (C20: 4) 0.8 eicosaenoic acid (C20: 1) 0.3 eicosatrienic acid (C20: 3) 0.4 eicosapentaenoic acid (C20: 5) 2,3 docosahexaenoic acid (C22: 6) 0.5 other fatty acids, sterols, carotenoids, cholesterol derivatives the rest is up to 100% 2. Product for external or internal use, including a biologically active additive and a filler, characterized in that as a biologically active additive it contains a lipid-vitamin complex from the extract of crushed cysts of Artemia Salina according to claim 1 with the following content of components, wt.% : lipid-vitamin complex from the extract crushed cysts of crustacean Artemia salina 0.01-30.0 filler for outdoor or internal use the rest is up to 100% 3. The product according to claim 2, characterized in that as a filler for internal use it contains sugar, or vegetable oil, or salt. 4. The product according to claim 2, characterized in that as a filler for internal use, it contains chalk and starch in a ratio of 2: 1. 5. The product according to claim 2, characterized in that as a filler for external use, it contains a gel base.