RU2297214C2 - Method for preventing and treating gastrointestinal diseases in animals and poultry - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: the innovation deals with peroral intake of enterosorbent as hydrolysis lignin in the form of the powder at fraction composition, weight portion of particles' size being below 0.5 mm - not less than 90%, 0.5-2 mm - not more than 5%, or hydrolysis lignin as aqueous suspension. Enterosorbent should be introduced once daily, not less, during 14 d running before feeding or together with the feedstuff. The innovation is simple in implementation, ecologically safe and highly efficient.

EFFECT: higher efficiency of prophylaxis and therapy.

6 cl, 3 ex

Description

The invention relates to the field of veterinary medicine and can be used as a highly effective and environmentally safe method for the prevention and treatment of gastrointestinal diseases with exo- and endogenous intoxications of various etiologies in agricultural and domestic animals and birds using veterinary enterosorbent based on lignin-containing raw materials.

Known complex feed microadditive for chickens (RU 2152157, A 61 K 1/16, 07.27.1998). It is a pharmacological agent and contains the following components: iron sulfate, cobalt sulfate, zinc sulfate, copper sulfate, sodium selenate, potassium bromide, rubidium nitrate, potassium iodide, cadmium nitrate, antimony trichloride, mercuric acid, nitric acid and nitric acid certain ratios. Feeding supplements with the main diet of chickens strengthens the immunobiological system of birds, which reduces the mortality of chickens.

The disadvantages of the known funds include the fact that it is a multicomponent composition containing, in particular, extremely dangerous and highly dangerous toxic chemicals (nitric acid mercury, antimony trichloride), which implies:

- the need for uniform distribution and high dosage accuracy when feeding the bird, which is difficult to achieve in industrial poultry farming;

- the danger of uncontrolled accumulation in the body of a bird of toxic substances for humans;

- mandatory compliance with certain safety measures and the use of personal protective equipment during the manufacturing process of the product;

- Observance of precautions during storage, transportation and use of this microadditive in order to prevent the harmful effects of toxic substances on the environment and human health.

Known therapeutic agent (RU 2081634, A 61 K 31/78, 11/25/1994). It is a premix tool for the prevention and normalization of deviations in the metabolism of animals, as well as for the treatment of deep metabolic disorders. As an active principle, premixes include a water-soluble polyelectrolyte complex of a cationic polyelectrolyte and an anionic detergent and a filler from a series including wheat bran, ground wheat or barley, irradiated fodder yeast, zeolite. In addition, mineral salts of micronutrients, vitamins and phosphorus-containing top dressings are introduced into the premix. The resulting premixes are introduced into the composition of the feed. Feeding to calves compound feeds with premixes in daily doses of 1-5 kg / 100 kg of live weight provides the prevention of metabolic disorders, as well as the therapeutic effect in severe metabolic disorders. Premix does not have antimicrobial activity, and therefore cannot be recommended for the treatment of gastrointestinal diseases associated with infection or intoxication.

The disadvantages of the known funds can be attributed to the fact that it is a multicomponent composition, which significantly increases its cost and, therefore, limits the range of consumers.

Known means and method of treating the prevention of pig dysentery (RU 2165756, A 61 K 31/00, 06/15/1999). The essence of the invention lies in the fact that the treatment and prevention of dysentery by oral administration of a medicinal product, which includes compounds of an organic and inorganic structure (10% water-soluble ronidazole and highly dispersed silicon dioxide). The drug is used once a day for three days in doses: Ronidazole 2.5-5.0 mg / kg body weight, highly dispersed silicon dioxide 10-20 mg / kg body weight. During treatment, the dose of the drug is dissolved in 100 ml of drinking water and is evaporated individually from a rubber bottle, and during prevention, the drug is fed in a group way.

The disadvantages of the known medicinal product of non-natural origin include:

- the possibility of an additional negative physiological effect during its use in the pathological condition of the gastrointestinal tract (GIT);

- the need for accurate dosage of the drug;

- the possibility of unwanted shifts in the composition of the microflora of the gastrointestinal tract;

- the possibility of drug resistance.

The tasks to which the invention is directed are to create a biocompatible, harmless and environmentally safe method for the prevention and treatment of natural enterosorbent (based on lignin-containing raw materials) of gastrointestinal diseases in animals and birds, which allows:

- apply it in the treatment of simple and associated infections, especially of unclear etiology, proceeding with severe clinical manifestations;

- apply it for atypically manifest forms of diseases, the diagnosis of which is difficult;

- quickly and effectively remove the effects of intoxication in the early stages of a gastrointestinal disease, which avoids dehydration of the body and subsequent complications;

- apply it in combination with the recommended treatment regimen with specific antiviral and antibacterial drugs (antibiotics and sulfonamides);

- apply it in order to prevent the accumulation of conditionally pathogenic microflora, which normally does not cause clinical manifestations of the disease;

- apply it prophylactically in order to neutralize anti-food substances often found in compound feed (secondary metabolites of microscopic fungi and molds, mycotoxins), which cause mass poisoning in industrial poultry and livestock farming;

- positively affect intestinal motility,

- to avoid the development of resistance of microorganisms to this factor of therapeutic effect;

- eliminate the irritating and traumatic effect of enterosorbent particles on the mucous membrane of the stomach and intestines due to the soft structure;

- minimize the risk of side effects.

The tasks are solved as follows.

A method for the prevention and treatment of gastrointestinal diseases in animals and birds involves the oral administration of an enterosorbent and is characterized in that hydrolyzed lignin in the form of a powder with a fractional composition is used as an enterosorbent; the mass fraction of particles less than 0.5 mm in size is at least 90%, 0, 5-2 mm - not more than 5%, or hydrolysis lignin in the form of an aqueous suspension. The introduction of enterosorbent is carried out at least once a day for no more than 14 consecutive days before feeding or mixed with food.

Enterosorbent in the form of a 10% suspension is prophylactically prodused once a day 50-60 minutes before morning feeding for 3-5 days to calves from the first day of life at a dose of 1 ml / kg of body weight.

Enterosorbent in the form of a 10% suspension is prophylactically prodused once a day 50-60 minutes before morning feeding for 3-5 days to piglets from 12 days of life at a dose of 2 ml / kg of body weight.

Enterosorbent is prophylactically administered to animals mixed with food for 7-14 days at a dose of 0.3-0.5 g / kg of animal weight or in an amount of 1% of the daily feed intake.

Enterosorbent is treated as a solution in the form of a 10% aqueous suspension for 2-5 days 2-4 times a day 50-60 minutes before feeding in a single dose - to calves - 200-300 ml / animal, to pigs - 50 ml / animal.

Introducing enterosorbent to the bird for 7-14 days in a mixture with feed in an amount of 1-2% of the daily feed intake.

Enterosorbent (veterinary polyphepan) is a natural product, which is a purified and activated lignin from coniferous wood, obtained by acid hydrolysis. It contains 34-37% lignin, 60% hydrocellulose and 1-3% minerals.

Veterinary polyphepan is a pH-neutral ground wood residue after its acid hydrolysis (hydrolysis lignin), purified from impurities and activated by alkaline treatment at elevated temperature, followed by washing from alkali and grinding.

Target Product Feature

Trade name of the drug: Veterinary Polyphepan

International nonproprietary name: hydrolysis lignin

Dosage form: powder for oral administration

Enterosorbent: Veterinary polyphepan is used for the prevention and treatment of gastrointestinal diseases, with exo- and endogenous intoxications of various etiologies in agricultural and domestic animals and birds.

The drug has the following physico-chemical characteristics:

Appearance: Amorphous loose powder of dark brown color without extraneous impurities.

Authenticity: The appearance of a pink-violet color when mixed with an alcoholic solution of phloroglucin, turning into purple.

PH of aqueous extract: From 6.0 to 7.0 potentiometrically.

Mass fraction of moisture: Not less than 60% and not more than 70.0%.

Mass fraction of ash (in terms of absolutely dry matter): Sulphated ash should not exceed 3% of dry weight.

Fractional composition: Mass fraction of particles in size:

less than 0.5 mm - not less than 90%,

0.5-2 mm - not more than 5%,

over 2 mm - not allowed.

Adsorption activity: Not less than 0.02 g of methylene blue per 1 gram of the drug, calculated on dry weight.

Contraindications: There are no absolute contraindications.

Veterinary polyphepan, having a large specific surface area (up to 15-20 m ² / g) and having a significant set of functional groups (methoxyl, carboxyl, hydroxyl), which determine the possibility of chemisorption and complexation, exhibits high sorption activity and nonspecific detoxification effect.

In the gastrointestinal tract, the drug absorbs, firmly retains and removes pathogenic microorganisms from the body (E. coli, Salmonella, Proteus, Staphylococcus, Yeast microflora, Pseudomonas aeruginosa), toxins secreted by them, as well as salts of heavy metals, nitrites, nitrates, an excess of some products metabolism causing the development of endogenous toxicosis. The drug has a positive effect on intestinal motility and has an antioxidant effect.

The drug does not replace or exclude the use of specific antiviral, antibacterial drugs and other means of complex therapy. In order to avoid a decrease in the pharmacotherapeutic effect due to absorption by enterosorbent in such cases, it is given 1 hour before the use of other drugs.

Clinical and production tests of the drug were carried out, showing its high therapeutic and prophylactic effectiveness, the ability to bind and excrete heavy metals, radionuclides, cholesterol, bilirubin, lipids, bile acids, urea, bacteria, toxins. So, production tests of enterosorbent on sucking piglets of 5-35 days of age showed that the use of the drug allows reaching the livestock safety up to 93%, while in the control group the death rate of piglets was 30%. Positive results are shown as a result of the use of enterosorbent in complex therapy in the treatment of dogs with viral enteritis. The treatment efficiency in the experimental group was 95.2%, in the control group - 68.3%. The use of enterosorbent in a group method with compound feed in the conditions of a poultry farm in young chickens with the aim of preventing diseases caused by mycotoxins contained in compound feed made it possible to achieve a 98.5% livestock safety (90% in the control group).

We have conducted studies of enterosorbent as a detoxifying and sorbing agent for the prevention and treatment of gastrointestinal diseases in agricultural and domestic animals and birds.

The studied animals and birds were orally administered enterosorbent - hydrolytic lignin in the form of a powder with a fractional composition, the mass fraction of particles with a size of less than 0.5 mm - not less than 90%, 0.5-2 mm - not more than 5%, or hydrolyzed lignin in the form of an aqueous suspension . Enterosorbent was administered at least once a day for no more than 14 consecutive days before feeding or mixed with food.

EXAMPLE 1

For the purpose of prophylaxis in farms unsuccessful for acute gastrointestinal diseases, the drug in the form of a 10% suspension was drunk once a day 60 minutes before morning feeding for 3-5 days: calves from the first day of life at a dose of 1 ml / kg of weight, piglets - from the 12th day of life at a dose of 2 ml / kg of body weight.

Older youngsters, the drug was prescribed in a mixture with food for at least 7 days (but not more than 14) at a dose of 0.3-0.5 g / kg of animal weight or in an amount of 1% of the daily feed intake.

Clinical observation of experimental animals and smear test results showed that as a result of the use of enterosorbent, metabolism is normalized, good digestion function is maintained, which contributes to the high productivity of animals.

EXAMPLE 2

For colibacteriosis and salmonellosis, 20 calves and 20 piglets, enterosorbent was used as a 10% aqueous suspension, which was fed 2-5 days 2-4 times a day 60 minutes before feeding, in a single dose: calves - 200-300 ml / animal piglets - 50 ml / animal.

Control tests showed that in most animals (70%), recovery occurred on the third day of treatment, and in individuals, the day after the end of the course of treatment.

EXAMPLE 3

With colibacteriosis and salmonellosis, 20 individuals of birds, enterosorbent was used for 7-14 days in a mixture with food in the amount of 1-2% of the daily feed intake.

Control analyzes showed that in most animals (80%), recovery occurred on the seventh day of treatment, in the remaining individuals, 12-14 days after the start of treatment.

The invention will find application in veterinary medicine for the prevention and treatment of metabolic disorders in animals and birds as a highly effective, environmentally friendly and cost-effective means.

Claims (7)

1. A method for the prevention and treatment of gastrointestinal diseases in animals and birds, including the oral administration of an enterosorbent, characterized in that hydrolysis lignin in the form of a powder with a fractional composition is used as an enterosorbent: mass fraction of particles less than 0.5 mm in size is not less than 90 %, 0.5-2 mm - not more than 5%, or hydrolysis lignin in the form of an aqueous suspension, and the introduction of enterosorbent is carried out at least once a day, not more than 14 days in a row. 2. The method according to claim 1, characterized in that prophylactically calves are fed hydrolyzed lignin in the form of a 10% suspension once a day for 50-60 minutes before morning feeding for 3-5 days from the first day of life at a dose of 1 ml / kg weight. 3. The method according to claim 1, characterized in that prophylactically the pigs are fed hydrolytic lignin in the form of a 10% suspension once a day for 50-60 minutes before morning feeding for 3-5 days from 12 days of life in a dose of 2 ml / kg weight. 4. The method according to claim 1, characterized in that prophylactically, animals are injected with hydrolytic lignin mixed with food for 7-14 days at a dose of 0.3-0.5 g / kg of animal weight. 5. The method according to claim 1, characterized in that the hydrolytic lignin is fed to the calves in the form of a 10% aqueous suspension for 2-5 days 2-4 times a day for 50-60 minutes before feeding in a single dose of 200-300 ml / animal. 6. The method according to claim 1, characterized in that the pigs are treated with hydrolysis lignin in the form of a 10% aqueous suspension for 2-5 days 2-4 times a day for 50-60 minutes before feeding in a single dose of 50 ml / animal. 7. The method according to claim 1, characterized in that the enterosorbent is administered medicinally to the bird for 7-14 days in a mixture with food.