RU2268036C1 - Disinfectant - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: the present innovation deals with disinfecting sanitary-technical equipment, means for taking care of patients, articles of medicinal indication and, also, for pre-sterilization purification and disinfection of medicinal equipment. The suggested disinfectant contains the mixture of quaternary salts of ammonium and tertiary amines at the ratio ranged 10:1 to 1:10, correspondingly. The preparation is active in area of pH being 4-9. Concentration of working solutions - 0.05-0.1% for gram-positive and gram-negative microorganisms and 0.5-1% for microbacteria of tuberculosis and viruses including viruses of hepatitis and HIV-infection. The innovation provides increased activity referring to gram-positive and gram-negative microorganisms, tuberculosis microorganisms among them, being of high virucidal activity.

EFFECT: higher efficiency of disinfection.

1 cl, 2 ex, 2 tbl

Description

The invention relates to the field of disinfection and can be used at health facilities, food, dairy, beer and soft drinks and other industries, as well as in medical, child care facilities, public utilities and catering establishments. The invention can be used for disinfection of premises, sanitary equipment, patient care items, medical devices, tableware and laboratory glassware, as well as for pre-sterilization cleaning and disinfection of medical devices.

Known cleaning compositions containing salts of Quaternary ammonium and pine oil with a high content of terpene alcohols (for example, US 5591708, 7.01.1997; US 5983819, 11.16.1999). Quaternary ammonium salts exhibit disinfecting properties, promote the dissolution of pine oil, and provide a detergent effect. Known compositions have a bactericidal effect and can be used to clean hard surfaces.

A disadvantage of the known compositions is the low disinfecting ability, which does not meet strict sanitary and medical requirements. Therefore, they cannot be used in medicine and the food industry.

Disinfectant formulations containing aldehydes as an active component are known. In particular, the disinfectant described in EP 66759, AO I No. 35/02, 12/15/1982, contains glutaraldehyde or other C2-C6 dialdehyde, water, alkali metal carbonate and bicarbonate, sodium hydrogen phosphate and sodium dihydrogen phosphate. Similar products with various inorganic salts as buffer solutions are described in RU 2183468, 06/20/2002 and RU 2033664, 06/09/1995. Such disinfectants are not active enough, have an unpleasant odor, and are toxic.

A disinfectant is known (P O 95818 C1, A 61 K 9/01, 10/30/1988) containing 60% quaternary ammonium salt (alkyldimethylbenzylammonium chloride), 10% essential oil and 30% betaine. The mixture is diluted in a ratio of 1: 5 with 80% ethanol. As essential oils, anise, lemon, pine, thyme, mint, lavender oils are used. The active components of these oils are oxygen-containing monoterpene compounds, in particular monoterpene alcohols C ₁₀ H ₁₆ OH (geraniol, linalool, terpineol, etc.). The disadvantages of this composition are low activity (working solution 2%) and insolubility in water. In addition, all of these oils, with the exception of pine, are expensive and inaccessible.

A disinfectant is known (US 4293809, 06/23/1988) containing a mixture of two quaternary ammonium salts (0.05-3.0% alkyldimethylbenzylammonium bromide) and 0.5-7.0% glutaraldehyde. The tool additionally contains 0.1-3.0% lower alcohol, 0.05-2.0% anti-corrosion additives (NaNO ₂ , KC O or K ₂ S ₂ O ₅ ), 0.1-3.0% surfactant (polyhydroxy compounds ), 0-0.015% complexone (salt or ether EDTU). The disadvantage of this composition is the lack of activity and increased toxicity due to the presence of glutaraldehyde in it.

A disinfectant is known (US 2666009, 09.09.1950 and US 2666010, 09.09.1950) based on quaternary ammonium salts containing pine oil or tert-octylphenylmonoethylene glycol ether or tert-octylphenyl diethylene glycol ether or fatty amine as a synergistic additive (promoter). . According to this work, the greatest sanergic effect is achieved in the presence of pine oil, the smallest in the presence of a fatty amine.

A disinfectant is known that contains 1-15% pine oil with at least 70% alcohol, 5-20% quaternary ammonium salt (acyldimethylbenzylammonium chloride), 1-15% isobornyl acetate, 10-20% surfactant (ethoxylated alcohols or alkyl phenols) and 5 -10% lower alcohol (ethanol and isopropanol), water - the rest (application RU 2000113057, 04/20/2002, A6II, 2/16, abstract). This solution is closest to the claimed invention. Active components (agents) are a quaternary ammonium salt (N-alkyl-N, N-dimethyl, N-benzylammonium chloride), pine oil and isobornyl acetate. The disadvantage of this tool is the need to use it in sufficiently high concentrations to achieve reliable disinfection, as well as the use of pine oil. This component is obtained synthetically from sulfate turpentine. For this reason, pine oil contains sulfur compounds that give it an unpleasant odor. In addition, due to unpleasant odor and strong fixation, this disinfectant is not suitable for pre-sterilization cleaning and disinfection of medical devices.

The aim of the present invention is the development of a disinfectant with increased activity against gram-positive and gram-negative microorganisms, including against mycobacterium tuberculosis and high virucidal activity.

Another objective of the invention is the provision of pre-sterilization cleaning and disinfection of medical devices.

The objectives of the invention are achieved by the fact that instead of terpene compounds (pine oil, isobornyl acetate, etc.), a tertiary amine is introduced into the disinfectant, i.e. the active substance (ADV) of the product is a mixture of a quaternary ammonium salt and a tertiary amine.

Example 1

The preparation of the disinfectant of the present invention is produced by mixing the components in the required proportions. The process is carried out in a reactor with a stirrer and reflux condenser.

A calculated amount of an aqueous solution of a salt or a mixture of quaternary ammonium salts is charged to the reactor, and then a calculated amount of a tertiary amine or a mixture of tertiary amines is added. The contents of the reactor are mixed, add acid (for example, hydrochloric, citric or phosphoric) to the desired pH of the solution. Then, the remaining auxiliary components are introduced: nonionic surfactant (ethoxylated fatty alcohols or phenols), lower alcohol (ethanol, isopropanol or a mixture thereof), complex (usually a salt or ether of EDTA, a salt of nitrilotrimethylphosphonic acid or a salt of hydroxyethylene diphosphonic acid), an anticorrosive additive (NaNO ₂ KClO. K ₂ S ₂ O ₅ ), dye, perfume and water to the required concentration of ADV in the disinfectant.

The auxiliary components of the disinfectant practically do not affect its bactericidal activity, but significantly change its specific properties, such as surface tension, foaming, wetting, antistatic and corrosion properties, washing ability, smell and color.

As a salt or mixture of quaternary ammonium salts, well-known bactericides are used, for example, N-alkyl-N, N-dimethyl-N-benzylammonium chloride, N-alkyl-N, N, N-trimethylammonium chloride, N-alkyl-N , N-dimethyl-N-ethylbenzylammonium chloride, N, N-dialkyl-N, N-dimethylammonium chloride and others, where is an alkyl hydrocarbon with 7-18 carbon atoms.

As tertiary amines or a mixture of tertiary amines of the present invention, amines having limited solubility in water are used, in particular such as:

N-alkyl-N, N-dimethylamine,

N-alkyl-N, N-diethylamine,

N-alkyl-N-methyl-N-ethylamine,

N-alkyl-N, N-dihydroxyethylamine, where

an alkyl hydrocarbon or a mixture of hydrocarbons with 7-18 carbon atoms, as well as amines of the type:

N-alkylbenzyl-N, N-dimethylamine,

N-alkylbenzyl-N, N-diethylamine,

N-alkylbenzyl-N-methyl-N-ethylamine,

N-alkylbenzyl-N, N-dihydroxyethylamine,

where alkyl is a hydrocarbon or a mixture of hydrocarbons with 2-12 carbon atoms. Many of these amines are intermediates in the synthesis of the above quaternary ammonium salts. If the amine is not sufficiently soluble in water, it is partially or completely converted to salt by treatment with hydrochloric, citric or phosphoric acid (Example 1) or a nonionic surfactant solubilizer is introduced. The type of acid used practically does not affect the bactericidal action of the disinfectant, for this reason the amine (amine mixture) is preferably used in the form of hydrochlorides.

Examples of disinfectant compositions of the present invention are given in table 1.

Example 2

The disinfecting activity of the agents was determined according to standard methods (Sat. "Test methods for disinfectants to assess their safety and effectiveness." M., Ministry of Health, 1998). For experiments used strains of Pseudomonas aeruginosa Esherichia coli M-17 and Staphylococeus aureus p-209 or No. 4. To assess the activity of disinfectants against mycobacterium tuberculosis, the strain M. smegmatis was used. The results of the study are given in table.2. Here are the results of determining the bactericidal activity of agents relative to E. Coly (MBK) in comparison with the bactericidal activity of the closest agent (MBK etc.), disclosed in the application RU 2000P3057, 05.25.2000 (prototype).

The above results (table 1, 2) indicate that the compositions of disinfectants based on a mixture of quaternary ammonium salts and tertiary amines of the present invention have significant advantages over the known.

a) The bactericidal activity of the proposed disinfectants for ADV is 2.5-10 times higher than the known ones (Table 2, column MBKpr / MBK).

b) Disinfectants exhibit the greatest bactericidal activity, in which the alkylbenzyl group is one of the substituents in the tertiary amine (Tables 1, 2, examples 13-16).

c) The proposed disinfectants of the present invention have a high bactericidal activity in both acidic and alkaline pH ranges (Tables 1, 2, Examples 2, 6), determined by the basicity of the amine.

However, due to the limited solubility of tertiary amines in water (working solutions become turbid), a neutral or slightly acidic pH range is preferred (4-8). The type of acid used to neutralize the amine (Tables 1, 2, Examples 2, 7, 8) practically does not affect the bactericidal activity of the disinfectant.

g) The bactericidal activity of the proposed disinfectants of the present invention is manifested in the range of the mass ratio of the Quaternary ammonium salt - tertiary amine from 10: 1 to 1:10 (Tables 1, 2, examples 1-5). The highest activity for a mixture of N-alkyl-N, N-dimethyl-N-benzylammonium chloride - N-alkyl-N, N-dimethylamine hydrochloride is observed at a mass ratio of the Quaternary ammonium salt - tertiary amine from 4: 1 to 1: 1 (Table. 1, 2, examples 2, 3).

Table 2. Disinfectant No.
according to table 1 E.Coly Staph.aureus M. smegmatis MBK Ave. The concentration of working solutions of disinfectants MBK 0.05% 0.1% 0.2% 0.05% 0.1% 0.2% 0.5% 1,0% 2.0% I. II. III. IV. V. VI. VII. Viii. IX. X. Xi. one. + + - + + - + + - 2,5 2. + - - + - - + - - 5,0 3. + - - + - - + - - 5,0 four. + - - + + - + + - 5,0 5. + + - + + - + + - 2,5 6. + - - + + - + - - 5,0 7. + - - + - - + - - 5,0 8. + - - + - - + - - 5,0 9. - - - - - - - - - 10.0 10. + - - + - - + - - 5,0 eleven. + - - + - - + - - 5,0 12. + - - + - - - - - 5,0 13. - - - - - - - - - 10.0 fourteen. - - - - - - - - - 10.0 fifteen. + - - + - - + - - 5,0 16. - - - - - - - - - 10.0

To impart specific properties to the disinfectant, as mentioned above, various additives can be introduced into it.

As an example, we give the full formulation of the medical disinfectant of the present invention:

N-alkyl-N, N-dimethyl-N-benzylammonium chloride (Catamine AB) 11.0-16.0% N-alkyl-N, N-dimethylamine in the form of hydrochloride 2.0-4.0% Neonol AF9-12 (ethoxylated alkalphenol) 0.5-2.0% Isopropanol 0.05-3.0% EDTA, sodium salt 0.01-0.4% Sodium nitrite 0.01-0.08% Water, up to 100%

The disinfectant has a high bactericidal activity (concentration of a working solution of 0.1% for the drug), including in relation to mycobacterium tuberculosis and viruses, including HIV viruses (concentration of a working solution of 0.8% for the drug). Disinfectant can be used for disinfection of premises of sanitary equipment, patient care items, medical devices, tableware and laboratory glassware, as well as for pre-sterilization cleaning and disinfection of medical devices.

Claims (2)

1. A disinfectant containing a quaternary ammonium salt or a mixture of quaternary ammonium salts, a nonionic surfactant, a lower alcohol complexon, an anti-corrosion additive, a dye and perfume, characterized in that the agent additionally contains a tertiary amine, or a mixture of tertiary amines, or their salts with acids when the weight ratio in the medium of the salt (mixture of salts) of Quaternary ammonium is the tertiary amine (mixture of tertiary amines) or their salts with acids, preferably from 10: 1 to 1:10. 2. The disinfectant according to claim 1, characterized in that as a tertiary amine (a mixture of tertiary amines) is used a) N-alkyl-N, N-dimethylamine; N-alkyl-N, N-diethylamine; N-alkyl-N-methyl-N-ethylamine; N-alkyl-N, N-dihydroxyethylamine, where an alkyl hydrocarbon or a mixture of hydrocarbons with 7-18 carbon atoms. b) N-alkylbenzyl-N, N-dimethylamine; N-alkylbenzyl-N, N-diethylamine; N-alkylbenzyl-N-methyl-N-ethylamine; N-alkylbenzyl-N, N-dihydroxyethylamine; where alkyl is a hydrocarbon or a mixture of hydrocarbons with 2-18 carbon atoms.